Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 52842347

GI number: 52842347

Start: 2377025

End: 2378293

Strand: Direct

Name: proP [H]

Synonym: lpg2133

Alternate gene names: 52842347

Gene position: 2377025-2378293 (Clockwise)

Preceding gene: 52842344

Following gene: 52842352

Centisome position: 69.96

GC content: 38.53

Gene sequence:

>1269_bases
TTGATTTATTGTATGGAAAATAAGAAGAAAAGGGTCGTTGTCGCAACCGCCGGAACTTTTCTGGAATGGGCTGAGTTTAC
TTATTACGCCTATATCGCCAATATTATCTCAGGATCGTTTTTTCCAAAATTGGGAAATCATCTTGGTTTGATAGCAACCT
TTTCTGTTTTCGCGCTCAGTTACTTTTTTCGTCCGTTAGGAGCCCTCTTTTTTGGATATCTTGGAGACAAGATGGGGCGT
CGAGTTGCCCTGCAATCTTCTATTATGCTGATGGGGTTATCCTCTCTGTTCATTGGTTGTCTTCCTGCATACAATAGTAT
TGGTATCTTGGCTCCAATTCTCTTGCTCATCTTTCGTTGTGTACAAGGCTTTGCCGTATCTGGTGAATTTAATGGTAGCG
CTATCTATCTGATTGAACACGACTCAGAAAAACCATGCCAGGCCGGTAGCTGGACTGGATTTGCTTCCGCATTGGGTATG
ATGTTTGGCGGATTGATGTCTACTTTAATTTATTTACCTCACATGCCAGAGTGGGCATGGCGTATCCCTTTTCTGTTAGG
AACCTTATCCTGCTTTTGTGCGATGTATTTTCGTAAAAATTTGAGGGAATCACCACCCTACCTCACAATAAGTAAAGAAA
AACCATCAAATCCACTGAAAATTTTATTTGACGATTATAAAAAACAATTAATTAAATCGGTATTATTAGTAGCGGCATTA
GGTATTTATATCTACATTATGAATGTTTATTATGCTTCTCATTTGGCAAAATACACCACTTTGTCAGATGCCCAAACCAA
ATTCGTTGTTACCCTAGGGCAAGGATTGGTTGTCCTATTCATTGTTTTAACAGGAATGTATGCGGATAGAACTGACGAAA
GACATATTATAAAAATCGGTTTATGGGGGTATTTTATTGCAGCTCCTATAGCTTACCTGGCCCCTCAAACCAACTCTTTT
GCATTAATTCTCTTATCACAAATTCCCTATGCAATCTGCAATGCTCTGGTCGGTACACCAATTTTTAAGCTATTGAATGA
TTTTTTTCCAACTCAGATTCGATACAGTGGTGTTTCTATTGCCTGGGGAGTAAGCATGGCTATTTTTGGAGGAACAGCGC
CATTGGTCGCCAATTATTTACAATATTCATTCAAATTAGCAACTGCACCAATATTCTATATACTTTTATCGGCCAGTGTT
GCCTTGCTCGTTTTACATGATAAAAAGAATTACAAGATAGCCCCGATAAAATTAAGATCAGATAATTGA

Upstream 100 bases:

>100_bases
TGACACTATAGTAACTCTATGAATCTGTTATATCATCGAATTACATAGAATAATGAGATCAATTTAAGGTAAAATCCCTC
TACATCACTTTAATATTCTA

Downstream 100 bases:

>100_bases
ATAAAATTAGCCTTTGCAAGCACATGTCTGTGCAAGCATCCAGGCAATTGGTGACGATTACTTCTTAATAGGTTCAATTA
TTCGATTCATCATGGTACTT

Product: proline/glycine betaine transporter-like protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYLGDKMGR
RVALQSSIMLMGLSSLFIGCLPAYNSIGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSEKPCQAGSWTGFASALGM
MFGGLMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLRESPPYLTISKEKPSNPLKILFDDYKKQLIKSVLLVAAL
GIYIYIMNVYYASHLAKYTTLSDAQTKFVVTLGQGLVVLFIVLTGMYADRTDERHIIKIGLWGYFIAAPIAYLAPQTNSF
ALILLSQIPYAICNALVGTPIFKLLNDFFPTQIRYSGVSIAWGVSMAIFGGTAPLVANYLQYSFKLATAPIFYILLSASV
ALLVLHDKKNYKIAPIKLRSDN

Sequences:

>Translated_422_residues
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYLGDKMGR
RVALQSSIMLMGLSSLFIGCLPAYNSIGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSEKPCQAGSWTGFASALGM
MFGGLMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLRESPPYLTISKEKPSNPLKILFDDYKKQLIKSVLLVAAL
GIYIYIMNVYYASHLAKYTTLSDAQTKFVVTLGQGLVVLFIVLTGMYADRTDERHIIKIGLWGYFIAAPIAYLAPQTNSF
ALILLSQIPYAICNALVGTPIFKLLNDFFPTQIRYSGVSIAWGVSMAIFGGTAPLVANYLQYSFKLATAPIFYILLSASV
ALLVLHDKKNYKIAPIKLRSDN
>Mature_422_residues
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHLGLIATFSVFALSYFFRPLGALFFGYLGDKMGR
RVALQSSIMLMGLSSLFIGCLPAYNSIGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSEKPCQAGSWTGFASALGM
MFGGLMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLRESPPYLTISKEKPSNPLKILFDDYKKQLIKSVLLVAAL
GIYIYIMNVYYASHLAKYTTLSDAQTKFVVTLGQGLVVLFIVLTGMYADRTDERHIIKIGLWGYFIAAPIAYLAPQTNSF
ALILLSQIPYAICNALVGTPIFKLLNDFFPTQIRYSGVSIAWGVSMAIFGGTAPLVANYLQYSFKLATAPIFYILLSASV
ALLVLHDKKNYKIAPIKLRSDN

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=30.3030303030303, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788292, Length=427, Percent_Identity=27.1662763466042, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=29.5508274231678, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789941, Length=450, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082231, Length=209, Percent_Identity=28.7081339712919, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082404, Length=368, Percent_Identity=24.1847826086957, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46952; Mature: 46952

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHLGLIATFSVFALS
CEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHH
YFFRPLGALFFGYLGDKMGRRVALQSSIMLMGLSSLFIGCLPAYNSIGILAPILLLIFRC
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VQGFAVSGEFNGSAIYLIEHDSEKPCQAGSWTGFASALGMMFGGLMSTLIYLPHMPEWAW
HCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RIPFLLGTLSCFCAMYFRKNLRESPPYLTISKEKPSNPLKILFDDYKKQLIKSVLLVAAL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIYIYIMNVYYASHLAKYTTLSDAQTKFVVTLGQGLVVLFIVLTGMYADRTDERHIIKIG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEE
LWGYFIAAPIAYLAPQTNSFALILLSQIPYAICNALVGTPIFKLLNDFFPTQIRYSGVSI
HHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCEEE
AWGVSMAIFGGTAPLVANYLQYSFKLATAPIFYILLSASVALLVLHDKKNYKIAPIKLRS
EHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEECC
DN
CC
>Mature Secondary Structure
MIYCMENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHLGLIATFSVFALS
CEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHH
YFFRPLGALFFGYLGDKMGRRVALQSSIMLMGLSSLFIGCLPAYNSIGILAPILLLIFRC
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VQGFAVSGEFNGSAIYLIEHDSEKPCQAGSWTGFASALGMMFGGLMSTLIYLPHMPEWAW
HCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
RIPFLLGTLSCFCAMYFRKNLRESPPYLTISKEKPSNPLKILFDDYKKQLIKSVLLVAAL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIYIYIMNVYYASHLAKYTTLSDAQTKFVVTLGQGLVVLFIVLTGMYADRTDERHIIKIG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEE
LWGYFIAAPIAYLAPQTNSFALILLSQIPYAICNALVGTPIFKLLNDFFPTQIRYSGVSI
HHHHHHHHHHHHHCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCEEE
AWGVSMAIFGGTAPLVANYLQYSFKLATAPIFYILLSASVALLVLHDKKNYKIAPIKLRS
EHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEECC
DN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA