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The map label for this gene is pilT

Identifier: 52842230

GI number: 52842230

Start: 2253222

End: 2254256

Strand: Reverse

Name: pilT

Synonym: lpg2013

Alternate gene names: 52842230

Gene position: 2254256-2253222 (Counterclockwise)

Preceding gene: 52842236

Following gene: 52842228

Centisome position: 66.35

GC content: 38.36

Gene sequence:

>1035_bases
ATGGATATCGCGGAATTATTGGCATTTTCAGTTAAAAACAAATCATCAGATTTGCATATTTCAGCTGGCATGCCTCCTAT
GATTCGTGTTGATGGTGATTTACGCAAAATTAATTTACCTCCATTAGAGCATAAAGACGTCATTAAGATTATTTATGACA
TAATGAATGACAGGCAAAGAAAAGAATATGAAGAATTTTTTGAAACAGATTTTTCCTTTGAAATAGCCAATCTGGCACGG
TTCAGGGTTAATGCTTTCAATCAATCGCGCGGTGCCGCTGCTGTTTTCAGAACTATTCCTTCTGAAATTCTTAGCATGGA
AGATTTAGGATTGCCCCCAATTTTTAAAGAAATGGCTAGTTTTTCCAGGGGTCTGGTTCTGGTTACTGGCCCAACTGGAT
CAGGAAAAAGTACCACGCTTGCAGCAGTCATCGATTATATTAATTCAAGTCGTTATGAGCATATTTTGACAGTTGAAGAT
CCCATTGAGTTTGTTCATCAAAGCAAAAAATGTCTGGTCAACCAACGCGAAGTACACAAAGACACATTGAGCTTTAATGC
CGCTTTGAGATCAGCGTTACGTGAAGATCCGGATATTATATTGGTGGGTGAATTACGCGATTTGGAAACTATACGCTTGG
CGATGACTGCAGCAGAAACCGGGCATTTGGTTTTTGGAACATTGCATACCAATTCTGCAACAAAAACAATAAACAGGATA
ATTGATGTATTTCCCGCTGAAGAAAAATCGATGGTACGCTCCATGCTTTCTGAATCATTGCAAGCTGTCGTTGCTCAAAG
TTTATTAAAAAAGAATAAAGGTGGTCGGGTTGCGGCGCTTGAAATTATGATGTGTACGGGGGCTATTCGTAATTTAATTC
GTGAGGATAAGATAGCGCAAATGTATTCGTCAATTCAAACTGGTCAAGCCAAAGGAATGCAAACATTGGATCAACATTTG
ACTGAATTGGTTAATAAAAATATTATTTCGAAACACACTGCTCATGAGGCTGCCCTGAACAAGTCATTATTTTAA

Upstream 100 bases:

>100_bases
AAGTGTAATGGTACTACAAAAAGATATCCATGATTGCTGGTAATCATTTCATGAGTTAATCTACAATATATAATGAAAAA
ATTGGCGGGAATTATAGAGT

Downstream 100 bases:

>100_bases
ATACACATGACTTGTGAAAAAGACCCAAGGTCGAGACAAAAAATGTTTTTAATGAGGAAGTTTATATAAATTAAATGACT
AAATTTAAAACATTATTTAA

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Sequences:

>Translated_344_residues
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF
>Mature_344_residues
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=318, Percent_Identity=45.9119496855346, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1786296, Length=287, Percent_Identity=33.7979094076655, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789723, Length=283, Percent_Identity=34.6289752650177, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38387; Mature: 38387

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
KEYEEFFETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMAS
HHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVEDPIEFVHQSKKCLVNQREVHK
HCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
DTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
MYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTAHEAALNKSLF
HHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
KEYEEFFETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMAS
HHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVEDPIEFVHQSKKCLVNQREVHK
HCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
DTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
MYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTAHEAALNKSLF
HHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]