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The map label for this gene is fliM

Identifier: 52842019

GI number: 52842019

Start: 1998302

End: 1999369

Strand: Reverse

Name: fliM

Synonym: lpg1792

Alternate gene names: 52842019

Gene position: 1999369-1998302 (Counterclockwise)

Preceding gene: 52842020

Following gene: 52842018

Centisome position: 58.84

GC content: 38.01

Gene sequence:

>1068_bases
TTGCTTATGACTGAGAAAGATGTGTTATCACAAGAAGAGATCGATGCATTACTGGATTCAGTGGATGAGTCAATTGATGA
TGAAACGAATGATGAAGAATTGAGTTCAAGAGATCAGCCAGTTCGTAAAAAACCAGAAGATACTACCAGCCAGGAATTAA
ACTCTAATTTTGATTCTGACAAAAAAGTAGCGGAAGAAGGAGTCAAAACACTTAATTTTACCGGACAAGAGCGTATTGTT
AAAGGACAGCTTCCGGTATTAGACAAAATTTATGACAGGGCAGTTCGTCTGTTTGCAGCAGATATTTATCATTTAACGGC
TAGAGATTTTGAAATTAAACAAGATCCGCTACTTATTACAAAGCATAAGGAGTTTATGAGGAGTTTACCTAATCCTAGCC
TGATAGGTATCTATAAATTCAAGCCTCTTCGTGGTAAAGGAATTATTTTGTTTGACAGTACCTTTGTGTATGACTTGGTC
GATTATTATTTTGGCGGAAATAGCCAGTTTGGTGCTCAAAAGGATAAAACAGATTTTACTGCTACTGAACTTCGTGTTAT
GGAGGTCGTAACTAAAAAACTGGTAACTAATTTAATTCATGCCTGTGAACCAATAATTCAACTGGATGTAACAAAATTCA
ATGACGAAACAAATCCCCAATTAGTTAATATTGCCGAGCCAGAAGAAATGTTGCTTGTGGCGCGGTTCGTCTTGAATTTT
GGTAAGGAAACCGGGGCTTTTTACTTTATTTTGCCTTACTCCATGCTTGAACCTATCAAACAGCAATTGGAGCTAGGGGC
ATCAAGACCTGATGATGAAATTGATCCTAACTGGATTAATTCTCTGAAAGAAGAACTCATGGACGTTGAATTGACGGTAA
GTGCTTCTATGGCGGAAACAGTCAGTACTCTGGGTCAGGTAATGGCTTGGAAAGTGGGAGATTTTGTCCCTTTGGAAATA
AACGAGGAGGTAACTCTGGATATAGAAGGGACACCAAGCTTTACTGCAACCTTAGGCAGTGCCAATGAAAAGCGAGCTTT
GAAAGTTATTAAAATAATACGTTATTAG

Upstream 100 bases:

>100_bases
AACCATTCTATAGTTGTTACTGATTGATTGAGCTAATTTTAAACTAATCAACACTTCAATTTTAAAATAACTTTGAAAAT
CTGGTTGAGATGCGAATGGA

Downstream 100 bases:

>100_bases
GGGATAAGGATGACTCAAGTGAACGAAACCAGCACAGAGCAATTAAATCAAGGGAATCCACTGAATCCAGATCCTGGCAA
TGAGAAATTGGAATTAATTT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLMTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPEDTTSQELNSNFDSDKKVAEEGVKTLNFTGQERIV
KGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLV
DYYFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFVLNF
GKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEI
NEEVTLDIEGTPSFTATLGSANEKRALKVIKIIRY

Sequences:

>Translated_355_residues
MLMTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPEDTTSQELNSNFDSDKKVAEEGVKTLNFTGQERIV
KGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLV
DYYFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFVLNF
GKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEI
NEEVTLDIEGTPSFTATLGSANEKRALKVIKIIRY
>Mature_355_residues
MLMTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPEDTTSQELNSNFDSDKKVAEEGVKTLNFTGQERIV
KGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLV
DYYFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFVLNF
GKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEI
NEEVTLDIEGTPSFTATLGSANEKRALKVIKIIRY

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=350, Percent_Identity=28.5714285714286, Blast_Score=147, Evalue=9e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 40250; Mature: 40250

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPEDTTSQELNSNFDSD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCH
KKVAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLIT
HHHHHHHHHEECCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEE
KHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDYYFGGNSQFGAQKDKTDFT
CCHHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCH
ATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFVLNF
HHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHC
GKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAET
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEEHHHHHHH
VSTLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTATLGSANEKRALKVIKIIRY
HHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLMTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPEDTTSQELNSNFDSD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCH
KKVAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYHLTARDFEIKQDPLLIT
HHHHHHHHHEECCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEE
KHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDYYFGGNSQFGAQKDKTDFT
CCHHHHHHCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCH
ATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQLVNIAEPEEMLLVARFVLNF
HHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHC
GKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNWINSLKEELMDVELTVSASMAET
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEEEHHHHHHH
VSTLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTATLGSANEKRALKVIKIIRY
HHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]