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The map label for this gene is pmbA

Identifier: 52841998

GI number: 52841998

Start: 1976586

End: 1978001

Strand: Reverse

Name: pmbA

Synonym: lpg1771

Alternate gene names: 52841998

Gene position: 1978001-1976586 (Counterclockwise)

Preceding gene: 52842004

Following gene: 52841993

Centisome position: 58.21

GC content: 40.32

Gene sequence:

>1416_bases
TTGCGAAGACATGTTATTGTATCCTTATGTACATCATTCAACACCACGGAATCTCATATGCAAATTAAACCGCAAAATAA
CAATAGTGAAGTATATAAGTCAACAACAGATTTAAATCGAGTAATAAAAGATGTTTTAGAACTTGCCAAAAAAGAAGGTG
CCACTGATGCAGCAGTAGCAGTAAATTATGATCGAGGTTTTTCAGTCGATGTGAGAATGGGGGAAGTGGAGACAGTCGCT
TTTAGCGAAGATAAAGGAGTTGGTTTAACCGTATACATAGGTCAGCGCAAAGGGGGAGCAAGCAGTACAGATACCTCTCC
TGAAGCATTACAATCTTTGGTTAAAGCAGCCTGTGAGATAGCGAAGGTTAGTGCCGAAGATCCTTGCTTTGGATTAGCTG
ATAAGGAATTGATGACAAAGAATCATCCAGATCTTGATTTGTATCATTCCTGGGACATTACCCCACAACACGCTATAGAT
TTGGCTTTAAAATGCGAATCACATGCCTTGTCTTTAGATAAACGGATTACTAATTCAGATGGTGTTAATGTTTCTTCCTA
TGAATCTCATCACGGTTATGCCAATACCCATGGTGGAGAGGGGTTTATTCATAGTACTCGACATAGCGTAAGTTGCTCGG
TAATCGCCAAAGAAGGTGATGAAATGCAAAGAGACTACGATTATACGACTGCCAGAAATGCAGAAAATTTAGTCTCAGCT
GATTTGATAGCAGCTAAAGCTGTTGAAAGGGCATTAAGTCGTCTGGGATCAAAACAAATTAAAACACAAATGACTCCGGT
TATTTTTTCTTCAAGAGTTTCCAGTGGATTATTGGGTAGCTTTGTGAGCGCTATTAGTGGTTCAAATTTATATAGAAAAA
ATTCCTTTTTGCTTGATTCCATTGGACAACAATTGTTTCCGGAGTTTGTTCATATTTACGAACAGCCACATTTAAAAGGT
GCTTTGGGGAGCGCCCCTTTTGATGGGGAAGGTGTGCCTACGCGACCCAATGTATTAGTTGAGAAGGGGCGCTTGCTTCA
ATATGTTCTTGGCAGTTATTCGGCGCGAAAAATGGGTTTAAGGACAACAGCAAATAGTGATGGCGTTCATAATTTAACCA
TTGATCCTACTTGCGGGGATCTTGGTGACCTTTTGAAAATGATGCGGAAAGGGTTGCTGGTTACAGAACTAATGGGTCAG
GGTGTGAATGGTATTACCGGAGATTATTCACGTGGGGCAAGTGGATACTGGGTTGAGGATGGGGTCATACAATATCCGGT
TGATCAGATTACTATCGCAGGAAATCTTAAAGAAATGTACCGGATGATATTGGCAGTAGGCAGTGACATTAATCCGAATA
TTTCCATGCGCTGTGGCTCTATTTTAATAGAAAATATGATGGTTGCAGGGAAATAA

Upstream 100 bases:

>100_bases
GCCAATCACTGCCAGCGTAGGAAGTCATAGGGATGACAATGGCGAGTGCTAAAACAAGCAAGAGCCATAAATTCAATTTG
TTTGCAGGATAAACATTGGT

Downstream 100 bases:

>100_bases
AATGCCCCAAACCATCGAGGTTAACCACTTGCTCTGTAATCTTGATGATAGTTCTCTGGAACATTTTTAGCGATATACCT
GGCTGCTTGCAGCCAGCTTT

Product: peptide maturation protein PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRRHVIVSLCTSFNTTESHMQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETVA
FSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAID
LALKCESHALSLDKRITNSDGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSA
DLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLKG
ALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQ
GVNGITGDYSRGASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK

Sequences:

>Translated_471_residues
MRRHVIVSLCTSFNTTESHMQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETVA
FSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAID
LALKCESHALSLDKRITNSDGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSA
DLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLKG
ALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQ
GVNGITGDYSRGASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK
>Mature_471_residues
MRRHVIVSLCTSFNTTESHMQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETVA
FSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAID
LALKCESHALSLDKRITNSDGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSA
DLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLKG
ALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQ
GVNGITGDYSRGASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=48.0984340044743, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789640, Length=397, Percent_Identity=23.9294710327456, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 51182; Mature: 51182

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHVIVSLCTSFNTTESHMQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVA
CCCEEEEEEHHCCCCCCCEEEEECCCCCCHHEECHHHHHHHHHHHHHHHHHCCCCCEEEE
VNYDRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEI
EECCCCCEEEEEECCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
AKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNSD
HHCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCHHHHHEEEEECCCHHHHHHHHCCCCC
GVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSA
CCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCHHHHHCCCCCHHCCHHHHHHH
DLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDS
HHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHH
IGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCHHHHCCC
RTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVED
EECCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEECC
GVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK
CEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRHVIVSLCTSFNTTESHMQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVA
CCCEEEEEEHHCCCCCCCEEEEECCCCCCHHEECHHHHHHHHHHHHHHHHHCCCCCEEEE
VNYDRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEI
EECCCCCEEEEEECCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
AKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNSD
HHCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCCHHHHHEEEEECCCHHHHHHHHCCCCC
GVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSA
CCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCHHHHHCCCCCHHCCHHHHHHH
DLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDS
HHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHH
IGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCHHHHCCC
RTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVED
EECCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEECC
GVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK
CEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]