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The map label for this gene is mpl

Identifier: 52841980

GI number: 52841980

Start: 1955401

End: 1956768

Strand: Reverse

Name: mpl

Synonym: lpg1753

Alternate gene names: 52841980

Gene position: 1956768-1955401 (Counterclockwise)

Preceding gene: 52841981

Following gene: 52841979

Centisome position: 57.59

GC content: 40.94

Gene sequence:

>1368_bases
ATGATGACACATTTGCATATACTAGGAGTAAGTGGAACCTTTATGAGTGCCTTGGCTTTGCTCGCCCGCGACGCAGGATA
TAGGGTGACAGGCAGCGATGCCAATTGCTATCCGCCTATCAGTGATTTGCTTGCTGCCAAAGGTATTTCATGGACTGAAG
GCTACGATGATTCCACTTTAGCATTGCAATCTGACATGGTTATTGTCGGTAATGCTATTAAGCGGGGAATGCCGGTTCTT
GAAGCAGTTATGGAGTCGGGAAAACCATATATGTCAGGCCCTCAATGGCTTGCCGAAAATATTTTATCAAAATATCAGGT
GATTGCGGTAGCTGGAACTCATGGCAAAACGACTACCACTTCAATGATTGCCTGGATTTTGGATCAAGCTGGAATGAATC
CGGGATTTCTCATTGGGGGCGTCTCTAGTGATTTCAATACCAGCGCCTGCCTTGGCAAAGGGAGATGGTTTGTCATAGAG
GCAGATGAGTACGATAGTGCTTTTTTTGATAAACGACCTAAATTTATGCATTATCGACCAAATATTGCAGTATTAAACAA
TTTGGAATTTGATCATGCCGATATTTATCCGGACTTGGCAGCTATTCAACAACAATTTCATTATTTATTAAGAACAATTC
CCAAAAGTGGTGTGGCTATCAAACCTCGAGATGATAAGGCTCTTAATGAAGTTCTATCACGAGGTCAATTTTCGCGTATT
GAAACACTGGCACTATCTGGAGAAGCCGAATGGGTTGCCGAACTGATTGACGATAGTGGTTCACAATTTAAAATACTCCA
TAATGGGGGAGTGGTTGCTGAAGTATCTTGGCCGTTGATAGGGCAATTCAATGTAGAAAATGGTTTGGCTGCAATTGCTG
CTACCTGGAATGCTGGAGTAAATCCTGAGGTTGCAGCCAAGGCTTTAGAACAGTTTTCTCCAGTGAAACGAAGGCTTGAA
GTTCGTTCCAATCGACATGGAATAACCGTTTATGATGATTTTGCCCACCATCCAACCGCAATTACTAAAACTATTGATGC
TTTAAAAAGAAGTGGACGACATCAACGTATTTTTGCTGTTATGGAGTTTGCTTCATATACTATGAGAACGGGTGTACATG
CCGCAGAAATGGCTCATGCTTTAGCTCCTGTTGATGGTGCCTACGTCCTGGAGCCTCAGGATTTTAATTTAAAAGATTTT
GTTAGCTCCTGGACTTGTCCATATAAAATTTGCAAAAACAATGGAGAGATAGTTAAAGAAATTATCAAAACAGTAAGAGA
AGGCGATGCTGTATTGGTAATGAGTAATAGAGGCTTTAATGGAATTCATCAACAATTGATCGATTCTATAGATAAACTTT
TTATGTAA

Upstream 100 bases:

>100_bases
ATTTATAAATTTGCTTTAGGCACTACACCGGTAAAATCAGTTTTAGCTGCTTTTGGTTTAGTCGCTGTTAACATTTTAAC
TATTTCTTTTTGGCGATGAC

Downstream 100 bases:

>100_bases
CGATTCATTTTTATTCTGAAATGTCTTGAGAACATATCATGATATTGAGAAACAGGTGAAATAAAAGTCATGTCCCATTA
TTTGCAAAGCCCCAACACAA

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVL
EAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRI
ETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVSWPLIGQFNVENGLAAIAATWNAGVNPEVAAKALEQFSPVKRRLE
VRSNRHGITVYDDFAHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAAEMAHALAPVDGAYVLEPQDFNLKDF
VSSWTCPYKICKNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM

Sequences:

>Translated_455_residues
MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVL
EAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRI
ETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVSWPLIGQFNVENGLAAIAATWNAGVNPEVAAKALEQFSPVKRRLE
VRSNRHGITVYDDFAHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAAEMAHALAPVDGAYVLEPQDFNLKDF
VSSWTCPYKICKNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM
>Mature_455_residues
MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVL
EAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRI
ETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVSWPLIGQFNVENGLAAIAATWNAGVNPEVAAKALEQFSPVKRRLE
VRSNRHGITVYDDFAHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAAEMAHALAPVDGAYVLEPQDFNLKDF
VSSWTCPYKICKNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=49.2239467849224, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786279, Length=405, Percent_Identity=29.8765432098765, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50072; Mature: 50072

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTL
CCEEEEEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEE
ALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTT
EEECCEEEECCHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHH
SMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPKFMHYRP
HHHHHHHHHCCCCCCEEEECCCCCCCCHHEECCCEEEEEECCCCCCHHHCCCCCCEEECC
NIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRI
CEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCHHH
ETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVSWPLIGQFNVENGLAAIAATWNAGV
HEEEECCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCEEEEEECCCCCEEEEEECCCCC
NPEVAAKALEQFSPVKRRLEVRSNRHGITVYDDFAHHPTAITKTIDALKRSGRHQRIFAV
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHH
MEFASYTMRTGVHAAEMAHALAPVDGAYVLEPQDFNLKDFVSSWTCPYKICKNNGEIVKE
HHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCHHHHCCCCHHHHH
IIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTL
CCEEEEEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEE
ALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTT
EEECCEEEECCHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHH
SMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPKFMHYRP
HHHHHHHHHCCCCCCEEEECCCCCCCCHHEECCCEEEEEECCCCCCHHHCCCCCCEEECC
NIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRI
CEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCHHH
ETLALSGEAEWVAELIDDSGSQFKILHNGGVVAEVSWPLIGQFNVENGLAAIAATWNAGV
HEEEECCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCEEEEEECCCCCEEEEEECCCCC
NPEVAAKALEQFSPVKRRLEVRSNRHGITVYDDFAHHPTAITKTIDALKRSGRHQRIFAV
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHH
MEFASYTMRTGVHAAEMAHALAPVDGAYVLEPQDFNLKDFVSSWTCPYKICKNNGEIVKE
HHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCHHHHCCCCHHHHH
IIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]