Click here to switch to the map view.

The map label for this gene is thiDE

Identifier: 52841798

GI number: 52841798

Start: 1734250

End: 1735737

Strand: Direct

Name: thiDE

Synonym: lpg1568

Alternate gene names: 52841798

Gene position: 1734250-1735737 (Clockwise)

Preceding gene: 52841797

Following gene: 52841799

Centisome position: 51.04

GC content: 38.31

Gene sequence:

>1488_bases
TTGGCATCAGGTAAACAATTTATGAAAAAACCGATTGTTTGGACAATTGCTGGTGTTGACTCATCCGGATTAGCTGGTGT
CCATGCTGACATGGAAACTTTTAGTCGCCTGAATGTCAGAGCTTGTTCAGTCATCACCGCAGTAACTGCACAAAATGCTC
ACTCAATTATAGCAGTAGAAGAGATATCCAGAGATCAGGTTGCTGCTCAGTGCCGTGCCTTGGAATTAAATTTAAAACCG
GATGCCATTAAAATTGGTATGTTATGTTCTACACCAATCTGTGAAGAAATAGCTTATTTCTTAAAGGGTTATGAAGGATT
TGTTGTTCTTGATCCGATAATTACCTCCTCCTCGGGAACTAACCTGTTTTTTCCAGATTTACAACAGCATAAAAATAATT
TAATTCAATTGTTTCCGTACATTACTATTATTACTCCTAATCGGATAGAAGCAGAAATTATTTTAAATCGGAGCATTTAT
TCTTATCAGGATATAATCAATGCTGCTTCAGATCTGTTATCACTTGGAGCAAAGCAGGTATTACTGAAAGGAGGACATGT
AAAAGATAACTCATTCAGTCAAGATTATTGGACTGATGGAAAAGAGTCGTTTTGGATTGCAAATCGTCGTTTTCCTGAAA
CAAATTACCGTGGGACAGGCTGTGTTCTATCTTCAGCACTCACAGCTTGTCTTGCTCTAGGCTACTCAATAAAAGATGCT
ATTGTTATTGCTAAAATGTATGTCAATCGTGGCATACGCCAATCAATAGAAATTGATAAAGACGCCTCGCAACTTTATCA
TGATGGTTGGCCAGAAGATGAGGCCGATCTCCCCTATTTATCACCAACACCGTTCATTAAACCAGTTCCTTCATTTAAAA
AATATTCTATGGGTTTTTATCCCATTGTGGATAGTAGCCACTGGTTAGAAATGCTTTTGCCATTGGGAATTAAGTGCATT
CAGTTACGAATAAAAGAAGCCTCTCAAGAGCGGTTGGAAGAAGAGATAAAACGGAGTGTCCATTTGGCAAATCAGTATAA
TGCTGCATTATTTATTAATGATCACTGGGAACTTGCTATTCATTATGGTGCTGCAGGAGTTCACCTTGGCCAAGAGGATC
TGGAAAAAGCTGATGTCGATAGAATTAATCGATCAGGTTTATTCTTAGGTATAAGTACGCATTGCTATTACGAAGTTGCT
CGGGCTCATGCTCTTAACCCTTCCTACGTCGCTTGCGGCCCCATATATGAAACAACCAGCAAAATAATGCCATTTCAAGC
ACAAGGAATTGCACGACTTGAACGTTGGCGAAAAACGCTTCGTTATCCATTAGTTGCCATTGGAGGTATAACTTTAAAGA
ACCTTTCAGATGTCTTAAAAACAAAAATTGATGGTGTGTCAGTGATCTCTGCCATTACAAAAGCATCAGCACCTTTAGTT
GCAGCCAAACAATTTTTAACTCAAATGAATGAATCACATAATGAATAA

Upstream 100 bases:

>100_bases
TCCGCCATGCTGTTATTGCAGGTCATCAGGCATTTACAGGAGGAATGATGTCTGAGAGAAATGTAGCTCACCCAAGTACT
CCATTAATCGATACTCCATT

Downstream 100 bases:

>100_bases
ATCAAATTTGGCAATGAACAAATTAACCCGCTATTCTCAACAAATAAAGATGGAAGAAATTGGATTAGACGGACAGGAGA
AACTTAAGAATTCTCGAGTA

Product: phosphomethylpyrimidine kinase ThiD/thiamin-phosphate pyrophosphorylase fused protein ThiE

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MASGKQFMKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSIIAVEEISRDQVAAQCRALELNLKP
DAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKNNLIQLFPYITIITPNRIEAEIILNRSIY
SYQDIINAASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDA
IVIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPLGIKCI
QLRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEVA
RAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLV
AAKQFLTQMNESHNE

Sequences:

>Translated_495_residues
MASGKQFMKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSIIAVEEISRDQVAAQCRALELNLKP
DAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKNNLIQLFPYITIITPNRIEAEIILNRSIY
SYQDIINAASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDA
IVIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPLGIKCI
QLRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEVA
RAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLV
AAKQFLTQMNESHNE
>Mature_494_residues
ASGKQFMKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSIIAVEEISRDQVAAQCRALELNLKPD
AIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKNNLIQLFPYITIITPNRIEAEIILNRSIYS
YQDIINAASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDAI
VIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPLGIKCIQ
LRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEVAR
AHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLVA
AKQFLTQMNESHNE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=204, Percent_Identity=44.6078431372549, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=35.2272727272727, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=29.2592592592593, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=270, Percent_Identity=30.3703703703704, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324517, Length=269, Percent_Identity=31.9702602230483, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 55082; Mature: 54951

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGKQFMKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSIIAVE
CCCCHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEHH
EISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGT
HHCHHHHHHHHHEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCEEECCCCC
NLFFPDLQQHKNNLIQLFPYITIITPNRIEAEIILNRSIYSYQDIINAASDLLSLGAKQV
EEECCCHHHHHCCCEEEECEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCHHHH
LLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDA
HHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
IVIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFY
HHHHHHHHCCCCHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEE
PIVDSSHWLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAI
EEECCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEE
HYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEVARAHALNPSYVACGPIYETTS
EECCCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCEEECCCHHHHH
KIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLV
HCCCCCHHHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCHH
AAKQFLTQMNESHNE
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASGKQFMKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSIIAVE
CCCHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEHH
EISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGT
HHCHHHHHHHHHEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCEEECCCCC
NLFFPDLQQHKNNLIQLFPYITIITPNRIEAEIILNRSIYSYQDIINAASDLLSLGAKQV
EEECCCHHHHHCCCEEEECEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCHHHH
LLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDA
HHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
IVIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFY
HHHHHHHHCCCCHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEE
PIVDSSHWLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAI
EEECCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEE
HYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEVARAHALNPSYVACGPIYETTS
EECCCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCEEECCCHHHHH
KIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLV
HCCCCCHHHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCHH
AAKQFLTQMNESHNE
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]