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The map label for this gene is folD

Identifier: 52841528

GI number: 52841528

Start: 1425272

End: 1426126

Strand: Reverse

Name: folD

Synonym: lpg1297

Alternate gene names: 52841528

Gene position: 1426126-1425272 (Counterclockwise)

Preceding gene: 52841529

Following gene: 52841527

Centisome position: 41.97

GC content: 40.35

Gene sequence:

>855_bases
ATGCCTGCATCACTAATCGATGGAAGAGAAATCTCTGCCCTTCGCAGAGCTGAACTAAAGCAAAGAGTACTATACCATGT
GGAGCAAGGGCAAAGGGCGCCTGGACTTGCTGTTGTTTTAATTGGCAATGACCCTGCATCAGTGATTTATGTCAGTAATA
AAAGAAAAGCTTGTGAAGAAGTAGGGATCACCTCTCATTCTTACGATTTGCCTGCCGAAACAACTCAGGAAAAATTAATT
GAACTGATCAATGAGTTAAATCAATCGGATAAAATTGATGGTATTTTAATCCAATTACCTTTGCCCAAACATATTAACGA
AAGAACTATAATAGAATACATAAAACCCGAGAAAGACGTGGATGGATTTCATCCTTATAATTTAGGCCGACTTGCTCAAC
GAAACCCGTTTTTAAGACCATGCACTCCCTTGGGGATTATGAATTTATTACATCACTATGAGCTCAATGTAAAAAGAAAA
CATGCTGTAGTGATTGGGGCATCCAATATTGTTGGAAGGCCAATGAGTCTGGAGTTACTATTAGCTGGTGCTACCGTAAC
AATATGCCACAAGTTTACCCAACAATTACAGAAGTTTGTTGAGATTGCTGATTTTCTAATAGTAGCCACTGGAAAGATGG
ACGTCATTGCCACTGACTGGCTCAGAGAGCATCAAGTAGTGATTGATGTAGGAATGCATCGTTTGCCCGATGGCAGTATC
AGGGGTGATATTGATTTTAAGAAGGCAGTCGAAAAAGTAGCCTGGATCACACCTGTTCCAGGGGGAGTTGGCCCAATGAC
TATTGTCACTTTGCTTGAAAATACTATGATGTCAGCAGCAAGATTAAGAGAATGA

Upstream 100 bases:

>100_bases
AACTGCAAGAATAAGTGTATTTTCCTTGTTGCATATGAATCATATTGCATTAGAATAACCCGTAATTCATAATGTACTCC
ATAAAAATTGCGAGAAGAAA

Downstream 100 bases:

>100_bases
AGTTATTTATCCAGTTCATCCCAATCAAATTCACCACTTAGCTCATCAAAATCGTCTTTGAACTCTTCTTTTAGACGCTT
ACGTTCAAGCCTATCCTCAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPASLIDGREISALRRAELKQRVLYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLI
ELINELNQSDKIDGILIQLPLPKHINERTIIEYIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE

Sequences:

>Translated_284_residues
MPASLIDGREISALRRAELKQRVLYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLI
ELINELNQSDKIDGILIQLPLPKHINERTIIEYIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE
>Mature_283_residues
PASLIDGREISALRRAELKQRVLYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLIE
LINELNQSDKIDGILIQLPLPKHINERTIIEYIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRKH
AVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSIR
GDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=40.8163265306122, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=40, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=40.6779661016949, Blast_Score=206, Evalue=1e-53,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=24.5, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=57.3476702508961, Blast_Score=337, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17568735, Length=296, Percent_Identity=39.1891891891892, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=256, Percent_Identity=43.75, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321643, Length=303, Percent_Identity=37.6237623762376, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=321, Percent_Identity=26.1682242990654, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=42.8571428571429, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=42.8571428571429, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=38.5665529010239, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31680; Mature: 31549

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASLIDGREISALRRAELKQRVLYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHH
VGITSHSYDLPAETTQEKLIELINELNQSDKIDGILIQLPLPKHINERTIIEYIKPEKDV
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCC
DGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRKHAVVIGASNIVGRPMSLELL
CCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECHHHHCCCCEEEEE
LAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
EECCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHCEEEEEECCCCCCCCCC
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE
CCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PASLIDGREISALRRAELKQRVLYHVEQGQRAPGLAVVLIGNDPASVIYVSNKRKACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHH
VGITSHSYDLPAETTQEKLIELINELNQSDKIDGILIQLPLPKHINERTIIEYIKPEKDV
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCC
DGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRKHAVVIGASNIVGRPMSLELL
CCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEECHHHHCCCCEEEEE
LAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIATDWLREHQVVIDVGMHRLPDGSI
EECCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHCEEEEEECCCCCCCCCC
RGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTMMSAARLRE
CCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA