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The map label for this gene is gloB [H]

Identifier: 52841526

GI number: 52841526

Start: 1423168

End: 1423932

Strand: Reverse

Name: gloB [H]

Synonym: lpg1295

Alternate gene names: 52841526

Gene position: 1423932-1423168 (Counterclockwise)

Preceding gene: 52841527

Following gene: 52841525

Centisome position: 41.91

GC content: 37.12

Gene sequence:

>765_bases
ATGACTATCCTGCCTATTTCGGCTTTTTCAGATAATTATATCTGGACCTTTATTGATAAAATAGCTGGGGTATTAGATTG
TGTTGATCCAGGAGAAGCAGCACCAATAATACGCTTTGCCCAATCCAACCAGCTGACACTAAGAACGATATTATTAACCC
ACCACCACTATGATCATATAGGAGGTGTTGACTCGCTAATCAAGCAATGGCCATCTTGTAAGGTCTATGGCCCCATAGAT
GAACGAATCAATAATGTAACCCACCCAATTAAACAAGGTCAATCTGTTCAAGTGGGCTCTCTTCACTTTCATATTTTATT
TAATCCAGGGCATACTTCTACCCATATCAGTTATTATGAACCACAACAAGGATGGTTATTTTGTGGAGATACTCTATTTT
CTGCTGGTTGCGGTAGAGTGTTTGATGGAACTATAGAGGAATTGCATGAATCCCTGCTCTTATTTAAAAAACTCCCGCGA
AATACTAAAGTTTTTTGTGCTCATGAATACACCTTCCAGAATTTAAAATTTGCACATACCGTTGAGCCCTGCAATTCATC
AGTCATCAATTACATGCAACAAATTCTGAAACAACCATCATCTTGTACTCTCCCGTCTAATATAGACTTGGAATTATCAA
TTAATCCTTTTTTACGCACTGATAAAGAGCAAGTAAAACAATATGCTCTCTCGCATGGTGCGAATTCTTCAGATTCTCTG
GACGTTTTTAAAGTATTGAGAAATCAAAAAAACTCATTCAAATAA

Upstream 100 bases:

>100_bases
AATCAAGTTAGTTTTAGAGACATTAACAACTTATTTTAACCCTGGCTCATCTTCTTATATGATTGAATCAGGTTCAAAAT
TATGTCACGAGTTATAAATT

Downstream 100 bases:

>100_bases
TATAACAAAAAGATTAAATAAAAACCCATTTGCTCCCAATTAAACCAAAAAGGGTTTAATCCTTCATTAATTTTTAGTAA
ACTGATTAGCCAATTATAAT

Product: hydroxyacylglutathione hydrolase GloB

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPID
ERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
NTKVFCAHEYTFQNLKFAHTVEPCNSSVINYMQQILKQPSSCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
DVFKVLRNQKNSFK

Sequences:

>Translated_254_residues
MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPID
ERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
NTKVFCAHEYTFQNLKFAHTVEPCNSSVINYMQQILKQPSSCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
DVFKVLRNQKNSFK
>Mature_253_residues
TILPISAFSDNYIWTFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPIDE
RINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRN
TKVFCAHEYTFQNLKFAHTVEPCNSSVINYMQQILKQPSSCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSLD
VFKVLRNQKNSFK

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=262, Percent_Identity=37.4045801526718, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI94538320, Length=262, Percent_Identity=37.4045801526718, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI14150041, Length=261, Percent_Identity=32.9501915708812, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=39.2070484581498, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI21703352, Length=227, Percent_Identity=39.2070484581498, Blast_Score=147, Evalue=6e-36,
Organism=Homo sapiens, GI46361987, Length=206, Percent_Identity=34.9514563106796, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1786406, Length=223, Percent_Identity=42.152466367713, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787158, Length=160, Percent_Identity=30, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=226, Percent_Identity=34.5132743362832, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6320478, Length=264, Percent_Identity=29.5454545454545, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324614, Length=249, Percent_Identity=28.5140562248996, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=263, Percent_Identity=39.1634980988593, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24667703, Length=263, Percent_Identity=39.1634980988593, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24667711, Length=263, Percent_Identity=39.1634980988593, Blast_Score=181, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28736; Mature: 28605

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHI
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCHHC
GGVDSLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYE
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEEEC
PQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRNTKVFCAHEYTFQNLKFAHT
CCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHCCCEEEEE
VEPCNSSVINYMQQILKQPSSCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCHH
DVFKVLRNQKNSFK
HHHHHHHHHCCCCC
>Mature Secondary Structure 
TILPISAFSDNYIWTFIDKIAGVLDCVDPGEAAPIIRFAQSNQLTLRTILLTHHHYDHI
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCHHC
GGVDSLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLHFHILFNPGHTSTHISYYE
CCHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCEEEEEC
PQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPRNTKVFCAHEYTFQNLKFAHT
CCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHCCCEEEEE
VEPCNSSVINYMQQILKQPSSCTLPSNIDLELSINPFLRTDKEQVKQYALSHGANSSDSL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCCCHH
DVFKVLRNQKNSFK
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA