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The map label for this gene is vsr

Identifier: 52841467

GI number: 52841467

Start: 1364344

End: 1364772

Strand: Reverse

Name: vsr

Synonym: lpg1235

Alternate gene names: 52841467

Gene position: 1364772-1364344 (Counterclockwise)

Preceding gene: 52841469

Following gene: 52841466

Centisome position: 40.17

GC content: 33.1

Gene sequence:

>429_bases
ATGGAGGGCAATTTTTTGGATATGATTAGTAAAAAACAACGTAGTCACATAATGAGCTCAATTAAAGGGAAAAATACTAA
ACCTGAAATAATTGTGCGTTCTTTTCTACACCGCCAGGGTCTTCGATTTAGATTGCATAGCAAGAACCTTCCTGGTCAGC
CTGATCTTGTGTTTAGAAAATACAACGCGGTTGTTTTTGTTAATGGGTGTTTTTGGCATGGTCATAACGAACCATTATGT
AAAAATTCTCATATCCCTAAAACAAATTTTGAATATTGGTGGAATAAGATTGAACGAAATAAGAGTCGAGATAAACAAAA
TATTGAGATATTAAAACAACTCGGTTGGCGTGTTTTTATGATTTGGGAATGCGAATTAAAAAATCATTGCTTTTTGTTGA
AACTATTAGCTTCTATAAAAAGCGAGTAA

Upstream 100 bases:

>100_bases
GTAAAGGTTACAGTTAATTTGGATCAAATATAATTTTTTTGAGGAGGTATCAATTTAAATCCTCACATTAATAAGCTTTT
TATATCTTATACTTTTGGAT

Downstream 100 bases:

>100_bases
TTCTATGTTGTGAAAATATTTTTTCCTCAGGATAAAAAACTATTTCCTAAAATTAAAAATAAGGATGGAAATTTATTTGA
GCTAATATTCCAATCTACAG

Product: patch repair protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MEGNFLDMISKKQRSHIMSSIKGKNTKPEIIVRSFLHRQGLRFRLHSKNLPGQPDLVFRKYNAVVFVNGCFWHGHNEPLC
KNSHIPKTNFEYWWNKIERNKSRDKQNIEILKQLGWRVFMIWECELKNHCFLLKLLASIKSE

Sequences:

>Translated_142_residues
MEGNFLDMISKKQRSHIMSSIKGKNTKPEIIVRSFLHRQGLRFRLHSKNLPGQPDLVFRKYNAVVFVNGCFWHGHNEPLC
KNSHIPKTNFEYWWNKIERNKSRDKQNIEILKQLGWRVFMIWECELKNHCFLLKLLASIKSE
>Mature_142_residues
MEGNFLDMISKKQRSHIMSSIKGKNTKPEIIVRSFLHRQGLRFRLHSKNLPGQPDLVFRKYNAVVFVNGCFWHGHNEPLC
KNSHIPKTNFEYWWNKIERNKSRDKQNIEILKQLGWRVFMIWECELKNHCFLLKLLASIKSE

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=122, Percent_Identity=41.8032786885246, Blast_Score=111, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16995; Mature: 16995

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGNFLDMISKKQRSHIMSSIKGKNTKPEIIVRSFLHRQGLRFRLHSKNLPGQPDLVFRK
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEE
YNAVVFVNGCFWHGHNEPLCKNSHIPKTNFEYWWNKIERNKSRDKQNIEILKQLGWRVFM
CCEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEE
IWECELKNHCFLLKLLASIKSE
EEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEGNFLDMISKKQRSHIMSSIKGKNTKPEIIVRSFLHRQGLRFRLHSKNLPGQPDLVFRK
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEE
YNAVVFVNGCFWHGHNEPLCKNSHIPKTNFEYWWNKIERNKSRDKQNIEILKQLGWRVFM
CCEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEE
IWECELKNHCFLLKLLASIKSE
EEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]