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The map label for this gene is eco47IIR [H]

Identifier: 52841469

GI number: 52841469

Start: 1366153

End: 1366905

Strand: Reverse

Name: eco47IIR [H]

Synonym: lpg1237

Alternate gene names: 52841469

Gene position: 1366905-1366153 (Counterclockwise)

Preceding gene: 52841470

Following gene: 52841467

Centisome position: 40.23

GC content: 32.01

Gene sequence:

>753_bases
TTGATATATGATGCTCATTTTATAATCCTCCAAGCCAGAAAAATGCCTTACGTTAGCTTTATTGATGATAATACTTTTGA
AAATATCGTTTCAGATACTATTAATGTTGCAAGTAATGCCATGTCTAATTCAAAAAAAAAATTTGAAAAGAATGTAATTG
ATCCTTTCTTAACTATTTTTGAAATTTCGGGATTTCGTATTGATTCAACAGAATGGTTGGAACATGAAAAATGGCGTCAA
GCACAAAAGTCTTTATCCAATAGTATCGGGATTTTTCATCAAAAACTATTAGGTAGTATAAATGGCTGGGAGTGTTTACC
AACAGGGCACATCGTAGACATAGTTAATCATGAAAAGCGAATAATTGCAGAAATTAAAAACAAGTACAATACAATTAAAG
GATCTGCCAAAGCAAAACTGTATCATGATCTTGACGATCTCGTGATGCAAAAAAAACAAGAATACAAAGAATACACTGCC
TATTATGTAGAAATTATTCCTAAAAAACCAGAAAGGTATAATCAACCCTTCACTCCATCAGATTCTCGTAAAGGAGCTAA
ATGTGCAGCAAATGAAAAAATCAGAGTCATTGATGGTTATAGCTTTTATAGCCTAGCATCAGGAATTCAGAATGCACTTG
AAGCCATTTATCAAGCATTACCTTTAGTGATTCAAAAAATTCTGCCTGATATAAATCTTGCTGAATTGCATAGTGTTATG
AAATATTTTAATCAGGCTTTCGGGAGCAAATAA

Upstream 100 bases:

>100_bases
GCCAATACCAAGCCATTTACTACATCTATCACTATGTACAATCTGAACTGAAAATTAATTCGTGCTAAAAATTCAACCAA
ATTTTGATTCAATTCGGTAA

Downstream 100 bases:

>100_bases
TTAGATCTTTCCTATCAAACCTCTGGGTAAGCATAGCAATAACACACCTGCCAATATGGTAGCCCAAGTTTACAGGCACA
GCATTACCGATTTGTTTATA

Product: type II restriction enzyme (Eco47II, Sau96I)

Products: NA

Alternate protein names: R.Eco47II; Endonuclease Eco47II; Type II restriction enzyme Eco47II [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIYDAHFIILQARKMPYVSFIDDNTFENIVSDTINVASNAMSNSKKKFEKNVIDPFLTIFEISGFRIDSTEWLEHEKWRQ
AQKSLSNSIGIFHQKLLGSINGWECLPTGHIVDIVNHEKRIIAEIKNKYNTIKGSAKAKLYHDLDDLVMQKKQEYKEYTA
YYVEIIPKKPERYNQPFTPSDSRKGAKCAANEKIRVIDGYSFYSLASGIQNALEAIYQALPLVIQKILPDINLAELHSVM
KYFNQAFGSK

Sequences:

>Translated_250_residues
MIYDAHFIILQARKMPYVSFIDDNTFENIVSDTINVASNAMSNSKKKFEKNVIDPFLTIFEISGFRIDSTEWLEHEKWRQ
AQKSLSNSIGIFHQKLLGSINGWECLPTGHIVDIVNHEKRIIAEIKNKYNTIKGSAKAKLYHDLDDLVMQKKQEYKEYTA
YYVEIIPKKPERYNQPFTPSDSRKGAKCAANEKIRVIDGYSFYSLASGIQNALEAIYQALPLVIQKILPDINLAELHSVM
KYFNQAFGSK
>Mature_250_residues
MIYDAHFIILQARKMPYVSFIDDNTFENIVSDTINVASNAMSNSKKKFEKNVIDPFLTIFEISGFRIDSTEWLEHEKWRQ
AQKSLSNSIGIFHQKLLGSINGWECLPTGHIVDIVNHEKRIIAEIKNKYNTIKGSAKAKLYHDLDDLVMQKKQEYKEYTA
YYVEIIPKKPERYNQPFTPSDSRKGAKCAANEKIRVIDGYSFYSLASGIQNALEAIYQALPLVIQKILPDINLAELHSVM
KYFNQAFGSK

Specific function: Recognizes the double-stranded sequence GGNCC and cleaves after G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019057 [H]

Pfam domain/function: PF09553 RE_Eco47II [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 28751; Mature: 28751

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYDAHFIILQARKMPYVSFIDDNTFENIVSDTINVASNAMSNSKKKFEKNVIDPFLTIF
CEECCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EISGFRIDSTEWLEHEKWRQAQKSLSNSIGIFHQKLLGSINGWECLPTGHIVDIVNHEKR
HHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEHHHHHHHH
IIAEIKNKYNTIKGSAKAKLYHDLDDLVMQKKQEYKEYTAYYVEIIPKKPERYNQPFTPS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCCCCCC
DSRKGAKCAANEKIRVIDGYSFYSLASGIQNALEAIYQALPLVIQKILPDINLAELHSVM
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KYFNQAFGSK
HHHHHHHCCC
>Mature Secondary Structure
MIYDAHFIILQARKMPYVSFIDDNTFENIVSDTINVASNAMSNSKKKFEKNVIDPFLTIF
CEECCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EISGFRIDSTEWLEHEKWRQAQKSLSNSIGIFHQKLLGSINGWECLPTGHIVDIVNHEKR
HHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEHHHHHHHH
IIAEIKNKYNTIKGSAKAKLYHDLDDLVMQKKQEYKEYTAYYVEIIPKKPERYNQPFTPS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCCCCCCC
DSRKGAKCAANEKIRVIDGYSFYSLASGIQNALEAIYQALPLVIQKILPDINLAELHSVM
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KYFNQAFGSK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607524 [H]