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The map label for this gene is citH [H]

Identifier: 52841397

GI number: 52841397

Start: 1282693

End: 1283979

Strand: Direct

Name: citH [H]

Synonym: lpg1163

Alternate gene names: 52841397

Gene position: 1282693-1283979 (Clockwise)

Preceding gene: 52841395

Following gene: 52841398

Centisome position: 37.75

GC content: 38.62

Gene sequence:

>1287_bases
ATGAATACATCAAATACCTCCTTTAAAACATTGGGAGCAATATGTATTGGTAATGCTATGGAATGGTATGATTTCATGAT
TTATAACTTCATGCTGCTTTTTATTGCCCAAGCTTTTTTTCCCAATCATAGTCCTGTGACTTCCCTCTTGGCTACAATGG
CAAGCTCTGGAGTGGCTCTGGTAGTCCGGCCTCTTGGTGGTTTTATTTTAGCAGTTTGGGCAGATAAGTATGGGCATCAT
AAAGCATTAATGTTCGTTTTTTATTTGATGGCTGCCGCAACCATGCTTATTACTTTTACACCATCCTACTCCACAATAGG
TCTGGTTGCTACTTGTGTCATTGTATTTGCGAGATTGTTGCAAGGACTTGCTACTGGTGGCGAGTTTGGCATATCCAGCG
CTCTATTAGTTAGCTTATCGCCAAGCGATAAAAAGGGGTTTTACACTTCCTTACAAATGGTAGGCCAGCTTTTGGCAATA
TTAATGGGATCTTCAGCGTGCCTTATTTTAACTTTCTATTGTTCCAATCAAACCCTTTATCAATTTGCCTGGAGAATACC
TTTTGCTTTAGGACTGTTGATTCTACCTTTAGGATGGATTTTAAGACGTCGATTGCAACGGGAATTTACCTGGAAGAAAG
AGCAACAGGACACAGGAAAGTTGTTTATCTTGGTAAAGCAACAAAAACGTTCTCTGTTGATAGCTTTTAGTCTTGTAGCC
GGGTGTACAGGCTCGATTTATACCTTATTCAGCTATATGCCTACCTATTGTAAATTGTACTTGAATTTATCGCTGACAGA
GGCGTACTTAGGCACTGCATCTGGAATATTAGTCAGTATTATTACTATTCCCTTTTTTGGAGCATTGTCGGATAGAATAG
GCAAAAAAATAGTTATGTTATGTGCTTTGGCTTGTTATTTTGGATTAATTTATCCTTTACTTTTCTTACTCAACCAATAC
CCTGGTGTAACTATTCTCATTTTGGTTGAAAACATTTTAGGTTTGTGTATCGGCGCTTATTTTGGAGTGTTGACCATTAC
GGTTAGCTCCTTATTTCCACCATCAGTACGTTCTACTTGTCTAGCCGTCAGCTATAATTCAGCTGTTCTGTTGTTTGGTG
GTTTTGCTCCATTTATTATAACGGCGTTGATTGAATACACTAAAAACCCTATGGTTTTTACTTATTATCAAATGACGACG
GTCTTTATCAGTTTGCTAGCAGTCCTTGCTTATCAGGAGGAAAGGAAGCTATCTGAGAATCAGCCAGAACATCTCGTTGC
AATTTAA

Upstream 100 bases:

>100_bases
AGTAAAATTAATATTGATCTTAGTTTCTTATAAAGCATGGTAATTTTTTTTATATTCTACTAGTATGTGCTTTGCTCTAA
ATTTACCCTCGTAAGGAAAC

Downstream 100 bases:

>100_bases
AAGGTAGGAATATGACAACTTTAGATTGGTTAAAACAACTCATTTCTTTTGATACTACTTCGCGGAACTCTAATTTATCA
TTGATTGAATATTTGGCGAA

Product: MFS transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVFYLMAAATMLITFTPSYSTIGLVATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILTFYCSNQTLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSIITIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLAYQEERKLSENQPEHLVAI

Sequences:

>Translated_428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVFYLMAAATMLITFTPSYSTIGLVATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILTFYCSNQTLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSIITIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLAYQEERKLSENQPEHLVAI
>Mature_428_residues
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPLGGFILAVWADKYGHH
KALMFVFYLMAAATMLITFTPSYSTIGLVATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILTFYCSNQTLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSIITIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLAYQEERKLSENQPEHLVAI

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=29.0993071593533, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=28.6713286713287, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=29.0993071593533, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=26.5588914549654, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87082404, Length=367, Percent_Identity=24.5231607629428, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47285; Mature: 47285

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPLGGFILAVWADKYGHHKALMFVFYLMAAATMLITFTPSYSTIGLVATCVIVFARLL
EEEHHCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSACLILTFYCSNQTLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHH
QFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSIITIPFFGALSDRIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
LAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLAYQEERKLSEN
EEEEECCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC
>Mature Secondary Structure
MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPLGGFILAVWADKYGHHKALMFVFYLMAAATMLITFTPSYSTIGLVATCVIVFARLL
EEEHHCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSACLILTFYCSNQTLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHH
QFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSIITIPFFGALSDRIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
LAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLAYQEERKLSEN
EEEEECCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]