Click here to switch to the map view.

The map label for this gene is potB

Identifier: 52841374

GI number: 52841374

Start: 1255029

End: 1255853

Strand: Reverse

Name: potB

Synonym: lpg1140

Alternate gene names: 52841374

Gene position: 1255853-1255029 (Counterclockwise)

Preceding gene: 52841375

Following gene: 52841373

Centisome position: 36.96

GC content: 37.94

Gene sequence:

>825_bases
ATGAAGGCTAAATCATCTGCACTGTATTTATTGTATTTTTGGCTAATTATTTTTGGCTTCATTCCTTTGTTAATGGTGCT
GGTTGCCAGCTTTTTATCCCAAGACAGTATTCATCTTGTTACCTTGCCATTGACATTGGAAAATTATTTTGACTTGTTTA
CTCCTGTTTTTGCAAAAATCCTTTTCCGATCCTTGCTGATAGCCTGTGTCACCACCCTTCTTTGCCTGCTCATCGCCTAT
CCCTTTAGTTATCTTATGATTAAATCAAAACACCAATCTATTTTATTGCTGTTGATTATCATACCTTTCTGGACAAGCTC
ATTAATAAGAACTTATGCTTTGATTGCTATCCTTAAATACAAAGGCGTGATAAATGCCCTCTTGCTGAAATTGCATTTGA
TTGATGTTCCCTTGTCACTACTCTATACCAATTTTGCGGTCGTTATTGGCTTGGTTTACAATCTTTTCCCTTTCATGGTC
CTACCCATTTTTACTAATATGGAACGGTTCGATTTCAGGTTAATTGAGGCAGCTAAAGATTTAGGGGCAAACAAGTGGTC
TATATTTACACGAGTATTTTTGCCTAACACAGCCCCTGGAGTCTTATCAGGTTGTTTGCTGGTCTTGTTACCAGCAATGA
CTTTATTTTACATTCCTAATGTATTAGGTGGCGCTCGTTCCATTTTATTGGGAAATTTAATCCAAAATCAATTCCTTGTC
CTGAGCGATTGGCCTCAGGGAGCCGCTACCAGTGTGCTATTAACTTCCGTTCTGCTTATTTTAATGTTCATTTTTCGGCG
ACAGAACAATGAGGTCATTCGATGA

Upstream 100 bases:

>100_bases
GCTTCGGAGTTTTTTGATGAAGACGATGATAAGCTGGAATACACTCTCCATGAAACCGTTTGGGTACAATGGTTGCCTGG
CTGGGAGGTTTTATTACCTT

Downstream 100 bases:

>100_bases
AAACAATGACACAGCGCCTGTTTCTTTCTTTAGTTTATGCTTTTCTTTATATCCCCATTGCAATACTGGTGCTCTATTCA
GTAAATGATGCCAAATTTTC

Product: spermidine/putrescine ABC transporter permease protein PotB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAY
PFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR

Sequences:

>Translated_274_residues
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAY
PFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR
>Mature_274_residues
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAY
PFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=46.3320463320463, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=41.5254237288136, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787713, Length=271, Percent_Identity=28.4132841328413, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31015; Mature: 31015

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHH
LFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMVLPIFTNMERFDFRLIEAAKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
CCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHH
LFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMVLPIFTNMERFDFRLIEAAKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
CCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]