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The map label for this gene is lipA

Identifier: 52840982

GI number: 52840982

Start: 814524

End: 815513

Strand: Direct

Name: lipA

Synonym: lpg0745

Alternate gene names: 52840982

Gene position: 814524-815513 (Clockwise)

Preceding gene: 52840981

Following gene: 52840985

Centisome position: 23.97

GC content: 42.02

Gene sequence:

>990_bases
ATGGGCAAATTGATCGATATTCCAATCGTTGTGGAAAGTGGACAAAAATATAAGACATCTCAGGGTGTGACAGCTATTAA
AGATGGAATAAAATCATCAGGTCAAGATCATGAGCGATTGCCAAAACCGAAGTGGCTTCGTATAGTCAATCATACAACTC
CAGCCTATAGCCAAGTTAAAGAGCAAGTGCAAAAACATCGTTTGGCAACAGTTTGTGAAGAAGCGAAATGCCCCAACATC
TCAGAATGTTGGTCTCATGGAACTGCAACTATTATGTTAATGGGAGCAGTATGTACCCGGGCTTGCCGATTTTGTTCTGT
GGATACAGGAAATCCGCATGGTTGGCTTGATGCAGAAGAACCTGAGAATACCGCAGAAACTGTGGCCTTAATGAATCTGG
ATTATGTGGTTTTAACCTCAGTTAACCGTGATGATTTACCTGATGGGGGGGCGAATCATTATGCCAAAACCATTAGAGCA
ATCAAAAAACGATCTCCCCGAACCAAAGTCGAAGCGCTAACCCCCGATTTTCAAGGAAGTGAAAGAGATGTTGCTGTATT
GTTAGATAGTGGCGTTGATGTGTTTGCGCAAAATGTAGAAACGGTTGAGCGCCTGACACACCCGGTAAGAGATAATCGAG
CGGGTTATCAGCAAACCTTGAATGTACTGGCTTTTGCTAAGAAATATCGACCGGATGTTCTGACCAAAACCAGTTTGATG
TTGGGTTTGGGTGAAACCGATGAGGAAATTATACAGACTATGGATGACCTTCGAACTCATCACGTGGATATTTTGACTTT
GGGACAATATTTGCAACCAACTAAAAATCATTTACCTATAGCACGTTACGTAACTCCTGAAACTTTTTCTGAATTAAGGC
AAATTGGTCTAAAAAAGGGCTTTTTTGAAGTGGCTTCAGGCCCATTAGTTCGATCAAGCTATAGGGCTGACCGAGTGTTC
AAACGGGATAATTTAGGGCTTGATGTTTGA

Upstream 100 bases:

>100_bases
TGGTAACAGATGATTTTCCTCTGATAACGATTTTTAAATGAACTTGATCTTATCTTTGTGATTTGGGATCATCATAACTC
TTTGTATTTCGGATGGCGTC

Downstream 100 bases:

>100_bases
AGTTAGTGAACAGCTTGTGTTTTTCGAATTGGCTATAAATAATTTTATTATTAGTTAAAGAAATTAGATTTTTTATTAAA
CCATTAAACCGTTCATGCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNI
SECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
LGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVF
KRDNLGLDV

Sequences:

>Translated_329_residues
MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNI
SECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
LGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVF
KRDNLGLDV
>Mature_328_residues
GKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNIS
ECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAI
KKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLML
GLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFK
RDNLGLDV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=53.4013605442177, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=54.5816733067729, Blast_Score=271, Evalue=7e-73,
Organism=Escherichia coli, GI1786846, Length=316, Percent_Identity=48.1012658227848, Blast_Score=287, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=295, Percent_Identity=50.8474576271186, Blast_Score=293, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6324770, Length=310, Percent_Identity=49.6774193548387, Blast_Score=306, Evalue=3e-84,
Organism=Drosophila melanogaster, GI221513272, Length=290, Percent_Identity=54.1379310344828, Blast_Score=318, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_LEGPA (Q5X703)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123140.1
- ProteinModelPortal:   Q5X703
- STRING:   Q5X703
- GeneID:   3117566
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0810
- LegioList:   lpp0810
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   KTKIPMG
- ProtClustDB:   PRK05481
- BioCyc:   LPNE297246:LPP0810-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36742; Mature: 36611

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVK
CCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHEEEECCCCHHHHHHH
EQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEE
HHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAIKKRSPRTKVEALTPDFQGS
CCCHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCC
ERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKG
EECCCCHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHH
FFEVASGPLVRSSYRADRVFKRDNLGLDV
HHHHHCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVK
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCHHEEEECCCCHHHHHHH
EQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEE
HHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAIKKRSPRTKVEALTPDFQGS
CCCHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCCCC
ERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLM
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKG
EECCCCHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHH
FFEVASGPLVRSSYRADRVFKRDNLGLDV
HHHHHCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA