Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 52840981

GI number: 52840981

Start: 812822

End: 814372

Strand: Direct

Name: pleD [H]

Synonym: lpg0744

Alternate gene names: 52840981

Gene position: 812822-814372 (Clockwise)

Preceding gene: 52840980

Following gene: 52840982

Centisome position: 23.92

GC content: 34.88

Gene sequence:

>1551_bases
ATGATTGATCAAGAATTAAAGAAAAAAATTGTTAACACAATTAGCCAATATGAGCACAAATGTGACAAGATGCAAAGTCA
AATTAATACGCTCAGAAGTGTGATTAATCAATTGATTATTAATCCGATTGGAATTAATTATGAGTTAGATGATCGCCTTT
CGCAATTAAGAAATTATTTAGATACTGAATTTGAACCTGCTGTCATAGAAAAAAAAGTGAAACGTTTGGCAGAAATACTT
TCCAAAATGCAAAGGAAAAAAGCGGAAAATTCTCGTTTAGTCAATGGACTAATCAAACAGGGAGTGGAATCCATTGGTCG
TATTGCCAATAAATCTCAGGATAAGAGGGCAGTTACAAAATTACAAAAAATGCTGGATACCGAGATGGAAAGCCATGCGA
TTTTGATTCGTTTTAATCAAGTCTTAACTCAATGTATTTCTTCTGTGATTAAGGAGATGGAGAAGTTACCACAAATTACA
AAAGTCGATACCCCCCAAAAAAGTTATCAGTCTGAGATTAGTTTAAAAGTAAATGACAGCTTGCAACAATTACTTAACCA
TTTATCTATCCCTCAGGACTTGGATTCCAAGAGGGGAGTCATAAAAACAAATTTGGAACATGCACTCACTGATGAAGAGC
TTAGCACTATTATTGACAATTTGACGGAATTAGTCGTTGAGTCATTTAATGTTGAGCAGAATCGATTCAAAGGGTTTCTT
CAACAGTTAACCGATCAGCTCAATGATTTTGAAATCTATTTAAGAGTATCAGGAGATGCTCGCAAAAAGGCAAGTGAAGA
CAGTCGTTTATTAGAGTGTGGGATTCAGGATAATCTTGATCAAATCAAGAATCATTTGGATACCTCTAAAACGATTGAGG
AATTATCCTCAAAGGTGAGCCAAAATATAAAAATGATTGGTGACAGGTTAAAAGAATATCGACACAATGAAAAAATTCGA
GAGGAAGAATATGCAAAGCAAGTATCCGAGTTGCAAGCTAGATTACAGGAATCAGAGCAAAATGCGGAAGAAATAAAAAA
TTTGCTTGCTTTTCAGAAATACAGAATTAACCACGACAGCTTAACAGGCTTGCCAAATCGGGAATCTTATGATGAGCATA
TCCTGGATTCATTCCAGCGCTGGAAACGAAGTTCAAGCGCATTGTCTCTTGCTGTGTGTGACATCGATCATTTTAAACGT
ATTAATGATAATTTTGGCCATCTTGCAGGAGATAAGGTTCTTAAAAAGGTAGCTATGATTTTTAAATCATCAATCAGAAC
GGTTGATTTTATCGCTCGAATAGGTGGTGAGGAATTTGTGTTTATTTTTGAACAAACCAGCAGCAAGGAAGCTTTTGGAA
TTTTGGAGAAATTACGTAAATTAGTTGAAGATTGTTTGTTTTATTATCGAGACAAAAAAGTCGATGTTACTGTTTCTTTT
GGGCTAACTACGGTAACCGAAGAAGATGATATTGAATCTTTATTTATGCGGGCAGACAATGCCATGTATAAAGCTAAAAA
TGCAGGAAAGAACAGGGTAGAAATATTATAG

Upstream 100 bases:

>100_bases
AAACCAAAAACCCTCCTTTTTGTGATGGATCGCATGCAAAGATTTTGCTTGAAGTGGTTAAAAAAAAGGCAAAACAAATG
AACAGGAAAGGAAGTCTTAA

Downstream 100 bases:

>100_bases
TGGAATATATTTCCAACAATTTTTAATTATACTATTTTCGGAGTAAATCCATGGTAACAGATGATTTTCCTCTGATAACG
ATTTTTAAATGAACTTGATC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQIT
KVDTPQKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYDEHILDSFQRWKRSSSALSLAVCDIDHFKR
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYRDKKVDVTVSF
GLTTVTEEDDIESLFMRADNAMYKAKNAGKNRVEIL

Sequences:

>Translated_516_residues
MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQIT
KVDTPQKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYDEHILDSFQRWKRSSSALSLAVCDIDHFKR
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYRDKKVDVTVSF
GLTTVTEEDDIESLFMRADNAMYKAKNAGKNRVEIL
>Mature_516_residues
MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQIT
KVDTPQKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYDEHILDSFQRWKRSSSALSLAVCDIDHFKR
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYRDKKVDVTVSF
GLTTVTEEDDIESLFMRADNAMYKAKNAGKNRVEIL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=36.0248447204969, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=36, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=32.7272727272727, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=37.1951219512195, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=31.0734463276836, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=34.9693251533742, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=36.9230769230769, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=30.379746835443, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787541, Length=183, Percent_Identity=31.1475409836066, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=36.1963190184049, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=157, Percent_Identity=34.3949044585987, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=203, Percent_Identity=32.0197044334975, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=247, Percent_Identity=26.7206477732794, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59648; Mature: 59648

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIGINYELDDRLSQLRNYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQITKVDTPQKSYQSEISLKVNDS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCEEEECHH
LQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVS
HHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHH
QNIKMIGDRLKEYRHNEKIREEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
LTGLPNRESYDEHILDSFQRWKRSSSALSLAVCDIDHFKRINDNFGHLAGDKVLKKVAMI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHH
FKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYRDKKVDVTVSF
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GLTTVTEEDDIESLFMRADNAMYKAKNAGKNRVEIL
CEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIGINYELDDRLSQLRNYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQITKVDTPQKSYQSEISLKVNDS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCEEEECHH
LQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVS
HHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHH
QNIKMIGDRLKEYRHNEKIREEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
LTGLPNRESYDEHILDSFQRWKRSSSALSLAVCDIDHFKRINDNFGHLAGDKVLKKVAMI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHH
FKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYRDKKVDVTVSF
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GLTTVTEEDDIESLFMRADNAMYKAKNAGKNRVEIL
CEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA