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The map label for this gene is nadE [H]

Identifier: 52840971

GI number: 52840971

Start: 801369

End: 802979

Strand: Reverse

Name: nadE [H]

Synonym: lpg0734

Alternate gene names: 52840971

Gene position: 802979-801369 (Counterclockwise)

Preceding gene: 52840973

Following gene: 52840969

Centisome position: 23.63

GC content: 39.54

Gene sequence:

>1611_bases
ATGAATGCACCCTTGACAATACTGATGGCTCAAATTAATCCAACAGTTGGAGCTATTGACGCGAATACAAAAAAAATTAT
TGATGTCATCCAAAACCACCAGGCAAACCATGATGTGATTATTTTTCCAGAATTAACTCTATCCGGTTATCCGGCGGAAG
ATTTACTCTTTCGAAAAGAATATCATGATAAAATCATGCTCAACCTCCAGGATATTCAGGATACTACCAAGGATTGTTAT
GTCATTGTCGGACATCCAATGATACACATTGGGGATTGCTACAATGGCTTCAGCATCTTTTATCAAGGAGAAAAAATCAG
AGCATATCACAAGCAAAAATTGCCAAATTATGGAGTTTTTGATGAAGCAAGATATTTTACTCCTGGTAAAAAAGATCCTT
GCGTCTTATCAATTAAAAACCATAAATTAGGGTTTTGCATATGTGAAGATTTATGGCAAAAGGGTCCTGTAGATGATTTA
CTTGACAGTGGCATTTCCATTTTAATCAGTTTAAACGCCTCCCCCTTTGATTATCGTAAATACCAGCTGAGAGAAGAGCT
TTTAAGATCTTATGCTAAACGGGGGGTGTACATTATTTATGTCAATCAAATTGGTGGTCAGGACGATTTGCTTTTTGATG
GGCAATCTTTAGCCATGGATAATCAAGGTATTATCCGTGCTCTGGCTCCTGCCTTTGAAGAAAGCTTATGTACTGTCAAT
ATAGACGGTAACAAAATAGACGGATCAAGCACCCCTTACCTCGACAAAGAAGCGCTTATTTATAAGGCACTGGTTTGTGG
TACCAGAGATTACGTCAGAAAAAACAATTTCCCCGGCGTACTGCTTGGGCTCTCCGGAGGCATTGACTCTGCCTTAACTT
TAGCAATAGCTGTCGATGCGCTCGGAGCAGAGCAAGTCCATGCTGTGTTGATGCCCTCACGATATACCGCGTCAATCAGC
AATGAAGATGCACTGGAACAATTAAGCAACCTTAAAGTTGCACATTCCATTATACCGATTGAACCCATGTTTCAATCTAT
GATTGAATCGCTTCATCCTGTTTTTAAAGACTTGCCACCAGATACCACGGAAGAAAATATTCAGGCAAGAATACGTGGGA
TGCTGCTCATGGCTTTGTCAAATAAAACAGGGAAAATGGTTCTTACTACCAGTAACAAGAGTGAAACAGCTGTAGGTTAT
GCCACTTTATATGGCGACATGGCCGGCGGATTTGCCGTATTAAAAGATGTACTGAAAACTCAAGTGTATGAATTAGTGAG
GTATAGAAACAAGATTTCATTGGTTATCCCAGAGCGAGTCATTACCAGACCCCCTTCTGCTGAATTAAAACCAAACCAGA
CAGATCAGGATAGTTTACCCGAATACGGTGAACTGGATGCCATTATCATGGCTTACATGGAACAGAATTTAAGTCCCTCT
GAAATCATTGCCAAAGGATTTAAACCTCAAGTGGTCAACAAAATCATTCAATTGATTAAACGTAATGAATACAAGAGACG
TCAGGCTGCACCAGGAATAAAGATAAGTAATCTGGCCTTTGGTAAAGACTGGAGATATCCTATCACTAATGGTTTTAATC
AATGCATTTGA

Upstream 100 bases:

>100_bases
AAACTTCTCTAATTAACCTGACTACCATCCTCTTCATCTGTAGCCATATCAATGCCCTCTAAGCTACAATTCCTATATAC
AAATTAATTGACAGATTTTC

Downstream 100 bases:

>100_bases
ATGCAATAACTGCCTTGAAATAAAATTCAAGGCAGTTATTGCATTTATTAATTAAATAGATCTTATAGAGAGACTATCAT
TTGGTTTTGAGTGTTGACCT

Product: glutamine dependent NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MNAPLTILMAQINPTVGAIDANTKKIIDVIQNHQANHDVIIFPELTLSGYPAEDLLFRKEYHDKIMLNLQDIQDTTKDCY
VIVGHPMIHIGDCYNGFSIFYQGEKIRAYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCICEDLWQKGPVDDL
LDSGISILISLNASPFDYRKYQLREELLRSYAKRGVYIIYVNQIGGQDDLLFDGQSLAMDNQGIIRALAPAFEESLCTVN
IDGNKIDGSSTPYLDKEALIYKALVCGTRDYVRKNNFPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASIS
NEDALEQLSNLKVAHSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSNKSETAVGY
ATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELKPNQTDQDSLPEYGELDAIIMAYMEQNLSPS
EIIAKGFKPQVVNKIIQLIKRNEYKRRQAAPGIKISNLAFGKDWRYPITNGFNQCI

Sequences:

>Translated_536_residues
MNAPLTILMAQINPTVGAIDANTKKIIDVIQNHQANHDVIIFPELTLSGYPAEDLLFRKEYHDKIMLNLQDIQDTTKDCY
VIVGHPMIHIGDCYNGFSIFYQGEKIRAYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCICEDLWQKGPVDDL
LDSGISILISLNASPFDYRKYQLREELLRSYAKRGVYIIYVNQIGGQDDLLFDGQSLAMDNQGIIRALAPAFEESLCTVN
IDGNKIDGSSTPYLDKEALIYKALVCGTRDYVRKNNFPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASIS
NEDALEQLSNLKVAHSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSNKSETAVGY
ATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELKPNQTDQDSLPEYGELDAIIMAYMEQNLSPS
EIIAKGFKPQVVNKIIQLIKRNEYKRRQAAPGIKISNLAFGKDWRYPITNGFNQCI
>Mature_536_residues
MNAPLTILMAQINPTVGAIDANTKKIIDVIQNHQANHDVIIFPELTLSGYPAEDLLFRKEYHDKIMLNLQDIQDTTKDCY
VIVGHPMIHIGDCYNGFSIFYQGEKIRAYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCICEDLWQKGPVDDL
LDSGISILISLNASPFDYRKYQLREELLRSYAKRGVYIIYVNQIGGQDDLLFDGQSLAMDNQGIIRALAPAFEESLCTVN
IDGNKIDGSSTPYLDKEALIYKALVCGTRDYVRKNNFPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASIS
NEDALEQLSNLKVAHSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSNKSETAVGY
ATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELKPNQTDQDSLPEYGELDAIIMAYMEQNLSPS
EIIAKGFKPQVVNKIIQLIKRNEYKRRQAAPGIKISNLAFGKDWRYPITNGFNQCI

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59910; Mature: 59910

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLTILMAQINPTVGAIDANTKKIIDVIQNHQANHDVIIFPELTLSGYPAEDLLFRKE
CCCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCCCEEEEECCEECCCCHHHHHHHHH
YHDKIMLNLQDIQDTTKDCYVIVGHPMIHIGDCYNGFSIFYQGEKIRAYHKQKLPNYGVF
HCCEEEEEEHHHHCCCCCEEEEECCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCCC
DEARYFTPGKKDPCVLSIKNHKLGFCICEDLWQKGPVDDLLDSGISILISLNASPFDYRK
CCCCCCCCCCCCCEEEEECCCCCEEEEHHHHHCCCCHHHHHHCCEEEEEEECCCCCHHHH
YQLREELLRSYAKRGVYIIYVNQIGGQDDLLFDGQSLAMDNQGIIRALAPAFEESLCTVN
HHHHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCHHEECCCCHHHHHHHHHHCCEEEEE
IDGNKIDGSSTPYLDKEALIYKALVCGTRDYVRKNNFPGVLLGLSGGIDSALTLAIAVDA
ECCCEECCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LGAEQVHAVLMPSRYTASISNEDALEQLSNLKVAHSIIPIEPMFQSMIESLHPVFKDLPP
CCHHHEEEEECCCCEEECCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
DTTEENIQARIRGMLLMALSNKTGKMVLTTSNKSETAVGYATLYGDMAGGFAVLKDVLKT
CCCHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCEEEEEEEHHHHCCHHHHHHHHHHH
QVYELVRYRNKISLVIPERVITRPPSAELKPNQTDQDSLPEYGELDAIIMAYMEQNLSPS
HHHHHHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
EIIAKGFKPQVVNKIIQLIKRNEYKRRQAAPGIKISNLAFGKDWRYPITNGFNQCI
HHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEECEECCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MNAPLTILMAQINPTVGAIDANTKKIIDVIQNHQANHDVIIFPELTLSGYPAEDLLFRKE
CCCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCCCEEEEECCEECCCCHHHHHHHHH
YHDKIMLNLQDIQDTTKDCYVIVGHPMIHIGDCYNGFSIFYQGEKIRAYHKQKLPNYGVF
HCCEEEEEEHHHHCCCCCEEEEECCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCCC
DEARYFTPGKKDPCVLSIKNHKLGFCICEDLWQKGPVDDLLDSGISILISLNASPFDYRK
CCCCCCCCCCCCCEEEEECCCCCEEEEHHHHHCCCCHHHHHHCCEEEEEEECCCCCHHHH
YQLREELLRSYAKRGVYIIYVNQIGGQDDLLFDGQSLAMDNQGIIRALAPAFEESLCTVN
HHHHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCHHEECCCCHHHHHHHHHHCCEEEEE
IDGNKIDGSSTPYLDKEALIYKALVCGTRDYVRKNNFPGVLLGLSGGIDSALTLAIAVDA
ECCCEECCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
LGAEQVHAVLMPSRYTASISNEDALEQLSNLKVAHSIIPIEPMFQSMIESLHPVFKDLPP
CCHHHEEEEECCCCEEECCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
DTTEENIQARIRGMLLMALSNKTGKMVLTTSNKSETAVGYATLYGDMAGGFAVLKDVLKT
CCCHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCEEEEEEEHHHHCCHHHHHHHHHHH
QVYELVRYRNKISLVIPERVITRPPSAELKPNQTDQDSLPEYGELDAIIMAYMEQNLSPS
HHHHHHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
EIIAKGFKPQVVNKIIQLIKRNEYKRRQAAPGIKISNLAFGKDWRYPITNGFNQCI
HHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEECEECCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]