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The map label for this gene is purH

Identifier: 52840705

GI number: 52840705

Start: 501696

End: 503285

Strand: Reverse

Name: purH

Synonym: lpg0460

Alternate gene names: 52840705

Gene position: 503285-501696 (Counterclockwise)

Preceding gene: 52840706

Following gene: 52840704

Centisome position: 14.81

GC content: 39.56

Gene sequence:

>1590_bases
ATGGCGAAAAAACAAATTTACTCTTCTTTCAAGCCACGACGAGCACTACTGAGTGTTTCCGATAAAAGAGGAATAAGAGA
ACTCGGTCAAGTCTTACATGACCAAGGAGTGGAATTGATAGCCACTGGAAACACAGCTGCCATATTAAGAGAACATAAAT
TACCCGTTACCGATGTCAGTGAGTGCACAGGATTTCCCGAAATAATGGATGGAAGAGTCAAAACCCTTCATCCAGCCATT
CATGCTGGTTTATTAGCCCGCGGAGAAGAAGACAGCCCAGTTTTAAGACAACATGGAATAAAACCTATTGATTTACTGAT
TGTCAATTTATACCCCTTTGAACAAGTCATTAACCACCCGGACTGCGATTTTAATAAGGCCATTGAAAACATTGACATTG
GTGGACCAGCAATGGTGCGAGCGGCAGCTAAAAATCATGCCCATACTTATGTCATAGTTGATCCAAATGATTACTCAAAA
TTAATTCATTATTTACAAGATCAGAAAGCACCTTCCCATTGGAATTTTGCGTTAGCTAAAAAAGCATTTGCCCATACTGC
AGCCTACGATGCCGCAATTGCAAATTATTTAACTACTTTGGATAATGATTATGTCCCAACTGGATTTCCCGACATACTAA
CCTGCCAATTTAATAAAATTACTGATCTTCGTTATGGTGAAAACCCCCATCAGCAAGCTATTTTTTATGCTGATAAAAAT
TCACATCCAGGCTCTCTAAGTACAGCAACCTTATTACAGGGAAAACAATTATCCTATAATAATATTCTTGATGCCGATGC
AGCTCTCGATTGTGTGAAATCATTTTCCAATGAAAAGTCGGTTTGCGTCATTGTAAAACATACCAACCCCTGTGGCATTG
CGCTATCCGACACATCACTTGATGCTTACTTAAAAGCCTTTCAAAGTGATCCGATATCCGCTTATGGTGGAATTATTGCT
TTCAATGGAACTCTGGATAGCGATACGGCAAAAGCAATATTAGAAAAACAATTTGTTGAAGTGATTATTGCACCTGATGC
CAATGAAGAAGCAAAAAAAATTCTGGCAACCAAAGAAAACACTCGTGTTCTTCTAACCGGTTTTTGGCAACAAGGTAATA
ATTTTAGATTAAGTATGAAGAAAGTTGATGGTGGTCTATTAGTACAAGAACACGATTCTCTTTCTCTGGAATCATGTGAG
CTACAAACCGTTACCCAGATAAAACCCACTGATGAACAACTACAAAATTTAATGTTCGCTTGGTTAGCAGCCAAGCATGT
CAAATCTAATGCCATTGTATACGCTAATGATTTGACCACTATAGGGATTGGCGGAGGACAAACCAGTCGAGTAATGAGTG
CTCGTATTGGTTTGTGGCAAGCAGAGCAAATGGGGTTTGATCCCAAAGGCGCTGTTATGGCCTCAGACGCATTTATCCCT
TTCCCTGATACCATTGAAATAGCAGCGAAGGCTGGTATATCCGCAATTATTCAACCAGGGGGCTCTATCAGAGATGAAAA
AATCATTTCTTGCGCCGACCAACACAACATAGCGATGATTTTTACAGGAGTGCGACATTTTAAACATTAA

Upstream 100 bases:

>100_bases
ATGATTCCGCATTCACCCATATTGCAACTGAATATTGTGAGGGATGGTCGTTGCTGGTTTTTACAAGCAAATAATAACTA
TATCACTAAGGTATATGTAA

Downstream 100 bases:

>100_bases
TGTCAAGCATGTATTAATTCTAAGCTTGACTAACTTGAACAAAGAGTCGTATATAACGTATCATCTTCTTTTTAATTGAG
GCTTTTTGATTAGCATAAAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAKKQIYSSFKPRRALLSVSDKRGIRELGQVLHDQGVELIATGNTAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAI
HAGLLARGEEDSPVLRQHGIKPIDLLIVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSK
LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
SHPGSLSTATLLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIA
FNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILATKENTRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHDSLSLESCE
LQTVTQIKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLTTIGIGGGQTSRVMSARIGLWQAEQMGFDPKGAVMASDAFIP
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH

Sequences:

>Translated_529_residues
MAKKQIYSSFKPRRALLSVSDKRGIRELGQVLHDQGVELIATGNTAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAI
HAGLLARGEEDSPVLRQHGIKPIDLLIVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSK
LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
SHPGSLSTATLLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIA
FNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILATKENTRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHDSLSLESCE
LQTVTQIKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLTTIGIGGGQTSRVMSARIGLWQAEQMGFDPKGAVMASDAFIP
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH
>Mature_528_residues
AKKQIYSSFKPRRALLSVSDKRGIRELGQVLHDQGVELIATGNTAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIH
AGLLARGEEDSPVLRQHGIKPIDLLIVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSKL
IHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKNS
HPGSLSTATLLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAF
NGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILATKENTRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHDSLSLESCEL
QTVTQIKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLTTIGIGGGQTSRVMSARIGLWQAEQMGFDPKGAVMASDAFIPF
PDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=475, Percent_Identity=34.7368421052632, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=53.3333333333333, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71985564, Length=606, Percent_Identity=30.03300330033, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI71985556, Length=321, Percent_Identity=23.3644859813084, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71985574, Length=304, Percent_Identity=24.6710526315789, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323056, Length=472, Percent_Identity=33.2627118644068, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=32.8482328482328, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24649832, Length=484, Percent_Identity=34.7107438016529, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57948; Mature: 57816

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQIYSSFKPRRALLSVSDKRGIRELGQVLHDQGVELIATGNTAAILREHKLPVTDVS
CCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHHCCCCCCCHH
ECTGFPEIMDGRVKTLHPAIHAGLLARGEEDSPVLRQHGIKPIDLLIVNLYPFEQVINHP
HHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEECCHHHHHCCC
DCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSKLIHYLQDQKAPSHWNFALAK
CCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHH
KAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCC
SHPGSLSTATLLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSL
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCH
DAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILATKEN
HHHHHHHCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCC
TRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHDSLSLESCELQTVTQIKPTDEQLQNLMFA
CEEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHH
WLAAKHVKSNAIVYANDLTTIGIGGGQTSRVMSARIGLWQAEQMGFDPKGAVMASDAFIP
HHHHHHHCCCEEEEECCCEEEEECCCCHHHHHHHHHCCEEHHHCCCCCCCCEEECCCCCC
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH
CCCHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
AKKQIYSSFKPRRALLSVSDKRGIRELGQVLHDQGVELIATGNTAAILREHKLPVTDVS
CHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEHHHCCCCCCCHH
ECTGFPEIMDGRVKTLHPAIHAGLLARGEEDSPVLRQHGIKPIDLLIVNLYPFEQVINHP
HHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEECCHHHHHCCC
DCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSKLIHYLQDQKAPSHWNFALAK
CCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHH
KAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCC
SHPGSLSTATLLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSL
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCH
DAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILATKEN
HHHHHHHCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCC
TRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHDSLSLESCELQTVTQIKPTDEQLQNLMFA
CEEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHH
WLAAKHVKSNAIVYANDLTTIGIGGGQTSRVMSARIGLWQAEQMGFDPKGAVMASDAFIP
HHHHHHHCCCEEEEECCCEEEEECCCCHHHHHHHHHCCEEHHHCCCCCCCCEEECCCCCC
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH
CCCHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA