Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 52840477

GI number: 52840477

Start: 254259

End: 255779

Strand: Reverse

Name: ndhF [H]

Synonym: lpg0222

Alternate gene names: 52840477

Gene position: 255779-254259 (Counterclockwise)

Preceding gene: 52840483

Following gene: 52840476

Centisome position: 7.53

GC content: 38.86

Gene sequence:

>1521_bases
GTGGATAAATTTTTAAATGGGTTATCGATTTCACTCATGCTATCGCCCTTGATAGCGATGCTATTCTATATCTTGTTAGG
AAAAACAAGAAATATTCAAGCAACTTATTACGGTACCACAGTTATAGGTTTTGGTTTTGGATTGAGTCTCTTGGCACTAA
TTGTTTCTGCAAATACTGTAAACCAAAACAGTTATTTTTTATTTCATGCCAGCACACTAAGCCTCTTGCTAAGCACATTA
ATATTATTCATCAGCTTTATTATCCACCGTTTTTCCCTACGATATATGCATGGTGACAAGTGCTACCGACGTTATTTTTT
CAAACTTTCAGCCATAACTTTAACAACTGTTGTGATGGCTTTAGCAGACAATCTTTTATTGTTCTGGCTTGCCTGGTCAG
CTAGTAACTTGCTCTTGATAAACCTCATGATTCATAAGTCACAATGGAAAGCAGCCTACAACTCAGGGATACTGACTTTA
AAAACACTATTACCAGGAAGCTTGTTGCTTATCACGGCATTTTGGATTCTTTTTACGTTAAACAATACTCTGTCAATTGA
AACAATAACCAGCGAAGTGAGCAATAAGTCATCCCTTGCTATTGCCGTGGCACTTGGGTTTATTATCTTAGCAGCGATGG
CACAATCAGCCATATGGCCTTTTCATCGCTGGCTAATGAGCTCATTAAACTCTCCAACACCGGTATCCGCTCTAATGCAT
GCTGGATTAGTTAATGGAGGGGGCTTTCTGATTGTTAAATTTGCTCCGCTGTTCGTAACGCAAGAAAATTTATTGACCAT
TTTATTCATTTTCGGCGCACTGACTGCTTTATTAGGCACCCTCTGGAAACTGTTGCAGTGGAATATCAAAAGTATGCTTG
CCAGTTCCACCATGGCACAAATGGGTTTTATGATGATGCAATGTGGCTTAGGACTTTTCCCTGCCGCTATCGCCCATCTA
TGCTGGCACGGCTTGTTTAAATCCTATCTTTTTCTCAATTCAGGCTCTGCCGTCCAACAAAAGAAATATCAAAATAAATC
ACGTCCTGAAAAATTGACAAACTTATTGACTGCATTTTTTGGAGGACTTAGTGGAATGTATGGCTTTGCCCTTATCACAG
GGAAACCCATCTTATCTCTGCATGCCTCCACCTTTCTTTTAGGTTTTGCATTCATCACAGGAACACAACTCACCCTGGGC
TTGATTGCATATGAGAAGCATTTCAAAAAAGTAATTTTAGCCTTGGCTTTAGTTTTCTCAATTGGAATTTTTTATGGGGG
AAGTATTCATCTCATCGAGTCACTCTTACCTAATCTAACAGGATTTCCTCAATACCAATTAAGCAGCTTACAAGTCTTAA
CTCTGATTATCTTCTTTTCCCTTTGGCTTTTATTCAGCATGGGGTTATTCAAAAAAATCAGAGAGACCAGACTTTGGGAT
TGGTTTTACATGTACATGTTTAACGCAAGTACGCCTCACCCCAAAACTATTACGGCAAATCGCCACACTTATTACTATTA
A

Upstream 100 bases:

>100_bases
AGTTTTTCTAATAGTTTAAATAATTATTTTTAATTTTACTTATATTAACATATCTAATATATTTCCACAATAATAACAGA
ATTTCTAATGGAGAATTAAT

Downstream 100 bases:

>100_bases
GAAAAGGAGTCAACATGAGTAGCCACATCACACTAATCAAAACTAACACAAGCAATGAAAATCAAATGACCCAATGCAAC
TACCCAGTCGATAATGATGT

Product: NADH dehydrogenase subunit 5

Products: NA

Alternate protein names: NADH dehydrogenase subunit 5 [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MDKFLNGLSISLMLSPLIAMLFYILLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTVNQNSYFLFHASTLSLLLSTL
ILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSLAIAVALGFIILAAMAQSAIWPFHRWLMSSLNSPTPVSALMH
AGLVNGGGFLIVKFAPLFVTQENLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHL
CWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFFGGLSGMYGFALITGKPILSLHASTFLLGFAFITGTQLTLG
LIAYEKHFKKVILALALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSSLQVLTLIIFFSLWLLFSMGLFKKIRETRLWD
WFYMYMFNASTPHPKTITANRHTYYY

Sequences:

>Translated_506_residues
MDKFLNGLSISLMLSPLIAMLFYILLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTVNQNSYFLFHASTLSLLLSTL
ILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSLAIAVALGFIILAAMAQSAIWPFHRWLMSSLNSPTPVSALMH
AGLVNGGGFLIVKFAPLFVTQENLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHL
CWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFFGGLSGMYGFALITGKPILSLHASTFLLGFAFITGTQLTLG
LIAYEKHFKKVILALALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSSLQVLTLIIFFSLWLLFSMGLFKKIRETRLWD
WFYMYMFNASTPHPKTITANRHTYYY
>Mature_506_residues
MDKFLNGLSISLMLSPLIAMLFYILLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTVNQNSYFLFHASTLSLLLSTL
ILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSLAIAVALGFIILAAMAQSAIWPFHRWLMSSLNSPTPVSALMH
AGLVNGGGFLIVKFAPLFVTQENLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHL
CWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFFGGLSGMYGFALITGKPILSLHASTFLLGFAFITGTQLTLG
LIAYEKHFKKVILALALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSSLQVLTLIIFFSLWLLFSMGLFKKIRETRLWD
WFYMYMFNASTPHPKTITANRHTYYY

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=287, Percent_Identity=29.2682926829268, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1788614, Length=282, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788831, Length=350, Percent_Identity=26.2857142857143, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788827, Length=229, Percent_Identity=27.9475982532751, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56634; Mature: 56634

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFLNGLSISLMLSPLIAMLFYILLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTV
CCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
NQNSYFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTLKTLLPGSLLLITAFWILFTL
HHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHH
NNTLSIETITSEVSNKSSLAIAVALGFIILAAMAQSAIWPFHRWLMSSLNSPTPVSALMH
CCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGLVNGGGFLIVKFAPLFVTQENLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQ
CCCCCCCCEEEEEEEHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHH
GGLSGMYGFALITGKPILSLHASTFLLGFAFITGTQLTLGLIAYEKHFKKVILALALVFS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGIFYGGSIHLIESLLPNLTGFPQYQLSSLQVLTLIIFFSLWLLFSMGLFKKIRETRLWD
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WFYMYMFNASTPHPKTITANRHTYYY
HHHHHHCCCCCCCCCEEECCCCEECC
>Mature Secondary Structure
MDKFLNGLSISLMLSPLIAMLFYILLGKTRNIQATYYGTTVIGFGFGLSLLALIVSANTV
CCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
NQNSYFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCYRRYFFKLSAITLTTVVMA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTLKTLLPGSLLLITAFWILFTL
HHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHH
NNTLSIETITSEVSNKSSLAIAVALGFIILAAMAQSAIWPFHRWLMSSLNSPTPVSALMH
CCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGLVNGGGFLIVKFAPLFVTQENLLTILFIFGALTALLGTLWKLLQWNIKSMLASSTMAQ
CCCCCCCCEEEEEEEHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNSGSAVQQKKYQNKSRPEKLTNLLTAFF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHH
GGLSGMYGFALITGKPILSLHASTFLLGFAFITGTQLTLGLIAYEKHFKKVILALALVFS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGIFYGGSIHLIESLLPNLTGFPQYQLSSLQVLTLIIFFSLWLLFSMGLFKKIRETRLWD
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WFYMYMFNASTPHPKTITANRHTYYY
HHHHHHCCCCCCCCCEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]