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The map label for this gene is yafV [C]

Identifier: 52840263

GI number: 52840263

Start: 10226

End: 11083

Strand: Reverse

Name: yafV [C]

Synonym: lpg0007

Alternate gene names: 52840263

Gene position: 11083-10226 (Counterclockwise)

Preceding gene: 52840264

Following gene: 52840262

Centisome position: 0.33

GC content: 42.66

Gene sequence:

>858_bases
TTGGGAACTCTAATGAAAGAAAACAAACTAACGGTCGCACTGGTTCAGGAACAATGGTATGAAAATCCTAAAGAACATCA
AGATAAACTGGCTTCTGGAATATTTTCAGCTACCCAGCAAGGGGCAAAACTCGTTTGTTTACAAGAGCTTACCTTAAGCC
CTTATTTCTGTACACGTAGTGACGTCGATCCCGCCCCTTATATGGAGGACATCGCCACTGGTCCCACTGCTCAATTTGTT
AGCCAAATGGCAAAGTCGAATCAAATTCATATTACCGCATCGCTTTTTGAAAAAGCTGGTTACAATACCGCAGTTGCTTT
TAATCCCCAAGGAGAATTGATTGCTGTTACCAGAAAGCAACATATCCCCAGTGGTGAAAAATATCATGAAAACTTTTACT
TCAAGCCGGGCAACTCCAATTATCCTGTCCATACCATTGCTGGTCACTACTTGGGATTACCAACATGCTATGACCAATGG
TTCCCGGAATTATCAAGGATTTATGGATTAAAAGGAGCTGAAATTTTGGTCTATCCCACAGCGATTGGAGGGGAACCGAC
TGCGCCAGGATTTGATAGCCAACCCATGTGGCAAAAAGTTATGGTAGCACAAGGAATTATGAGTAACACATTCATTATTG
CGGTCAACCGGATTGGGTGTGAAGATGGTTTAAGTTTTTACGGAAGCAGCTTTATCAGTACCCCTATGGGCGAAATTTTA
GTTCAGGCACCGCGTAATGAACCTGCCGTATTGGTTGCTGAACTGGATTTTAGCCAACGTGAATTGTGGGGGCGATTATT
TCCATTTCCCCAACAACGAGAGCCTGAAACTTATCATGAATTAGTCAAATCCCGTTAA

Upstream 100 bases:

>100_bases
TTTTTGATAACATAGCCAGATTTTTAATCTGGGTTGATGATTTAACTTAAGCAAGAGTAAGCCATTCGAATTCGAGATAA
AAATTACTTAACATCATTTA

Downstream 100 bases:

>100_bases
TCCTCTTTGGAACGTTAGAATGAACAATAATACTCTATCAGATGACAATCTTTACAACATAGAAAATTGGGGCGAGGGAT
ACTTCCGAATTAACTCCAAG

Product: hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MGTLMKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDPAPYMEDIATGPTAQFV
SQMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQW
FPELSRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEIL
VQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR

Sequences:

>Translated_285_residues
MGTLMKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDPAPYMEDIATGPTAQFV
SQMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQW
FPELSRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEIL
VQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR
>Mature_284_residues
GTLMKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVS
QMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQWF
PELSRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEILV
QAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=245, Percent_Identity=28.1632653061224, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI9910460, Length=281, Percent_Identity=28.8256227758007, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI297632350, Length=288, Percent_Identity=28.125, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI297632348, Length=288, Percent_Identity=28.125, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI5031947, Length=288, Percent_Identity=28.125, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=266, Percent_Identity=30.4511278195489, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=27.8523489932886, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358471, Length=264, Percent_Identity=29.1666666666667, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355835, Length=285, Percent_Identity=24.9122807017544, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17933642, Length=207, Percent_Identity=29.4685990338164, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31882; Mature: 31750

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLMKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRS
CCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCC
DVDPAPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQ
CCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEHHHHHHCCCCEEEEECCCCCEEEEEEHH
HIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQWFPELSRIYGLKGAEILVYPT
CCCCCCHHCCCCEECCCCCCCCEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEE
AIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEIL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHE
VQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR
EECCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
GTLMKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRS
CCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCC
DVDPAPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQ
CCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEHHHHHHCCCCEEEEECCCCCEEEEEEHH
HIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQWFPELSRIYGLKGAEILVYPT
CCCCCCHHCCCCEECCCCCCCCEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEE
AIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEIL
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHE
VQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR
EECCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]