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The map label for this gene is aceF

Identifier: 161723253

GI number: 161723253

Start: 1662037

End: 1663671

Strand: Reverse

Name: aceF

Synonym: lpg1503

Alternate gene names: 161723253

Gene position: 1663671-1662037 (Counterclockwise)

Preceding gene: 52841734

Following gene: 52841732

Centisome position: 48.96

GC content: 37.06

Gene sequence:

>1635_bases
ATGACAAAAGAAAGTGAAATTAAAATTCCTGATATCGGTGGCGCTAATCAAGTTGATGTCATTGAGATTTTAGTGAAAGA
GGGTGATCAGATCGAAGTAGATACACCATTGATTACGCTTGAGTCTGAGAAGGCAAGCATGGACATCCCTTCTCCAATAT
CTGGAACGGTAACGCAAATTCTCGTTAAAGTCGGTGACAAAGTATCCGAAGGTGATCTCATTGTCAAAGCCAAGTCAGAT
ACAACGACAAATATTTCCAGTTCTCAAGAACAAAAGACTGAACCAGAAAAACAGAATTTGCAAACCAGAGCAGAAGAGCA
ATCTGTTGATACAAAGGCAACTGCATCTACACCTAGTTCCAAAGATATTGAAATATCTATCCCGGATATTGGTGGTGCAA
ATGACGTTGATGTAATTGATATTTTAGTCAAACCAGGGATGGAAGTAGAAAAGGATCAGGCATTAATTACATTAGAGGGT
GATAAAGCAACGATGGATATTCCTTCGCCCTATGCAGGTAAAGTCATCGAAATGAAAATTAAATTAGGGGATAAAGTATC
TCAAGGTACTCCTATTCTAACTCTAAAAACCCTAGGAAAATCTGAGACACCTGAAATCGAAAAAAGCCAGATTAAAAATA
TCTCAGAACAATCTATAAAGGAAATAGAGAAGCCATATGAAGAACTAAAATCCGAAGCAATATCAATTAATAACCTTGAG
ATAGCTGAATCAAAAAGTATCTTAATATCTGCAGGTCCAGCCGTAAGAAGGTTGGCAAGAGAGTTTGGAGTCGATTTATC
TTTGGTTCAGGGAAGTGGGAGAAAATCTCGTATTACAAAAGAAGATCTGCAAAATTATATCAAAGTACGTTTAAGTGAAA
AAACTACGTCTGGTGGCTTCAGCTTACCGTCAAATCCAGCCATCGATTTCAGTAAATTTGGTTCAATTGAAACAAAGCCT
TTAAATAAAATTAAAAAACTTACTGGAACAAACGTACATCGCTCCTGGATAACCATTCCACATGTCACTCAATTTGACGA
AGCAGATATTACAGACTTGGAAGCGTTTAGAAAATCAGAATCAGAGAGCGCTAAAAATCAAGATTACAAATTAACCCTGT
TAGCATTTGTTTGCAGCGTAGTTTGTAAGGCTCTCCATGCTTACCCGCAATTTAATGCGTCACTAGATACCAGTGGTGAA
AATTTAATTTATAAAAAATATTATAATATAGGTATAGCAGTAGATACACCTAACGGTCTGGTGGTTCCTGTAATAAAGAA
TGTTGATAAATTGAGTGTGATTGATATTGCAAAGGAAATGTCCAGACTCAGTACGAAAGCTAGAGAAAAGGGATTAACAC
CTATTGATATGTCAGGCGGTTGTTTTACAATTTCCAGCCTTGGTGGTATAGGCGGAACGGCATTTACTCCTATTGTAAAT
AGCCCGGAAGTTGCAATTTTGGGCTTATCAAGATCAATAATAAAACCCATATACGATAATAAAGAGTTTAAACCCAGATT
GATGTTGCCTATTTCACTTTCTTACGATCATAGAGTAATCGACGGCGCTGAGGCAGCCCGGTTTACTCGCTTTCTTTGTG
ATTGTTTAGGTGATATTAGAAGAGTTTTACTCTGA

Upstream 100 bases:

>100_bases
TTACAATATTAATCAAGACAAATTGGATCCAATGACCCATTAGTTAAACAATTAATCAGAACAGAAGATAGTTCCGGTTT
AACTGAGTGCTGAGGAAAAT

Downstream 100 bases:

>100_bases
CATATAAAGGAATAGAGCTATTGGTATTTCCTTGTTCTGAATGAAATGCCAATAACCCCAGAAAACATATTTTTTGATGA
GGCTTCTTATGGCTAATAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSD
TTTNISSSQEQKTEPEKQNLQTRAEEQSVDTKATASTPSSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTLGKSETPEIEKSQIKNISEQSIKEIEKPYEELKSEAISINNLE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKP
LNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDTSGE
NLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL

Sequences:

>Translated_544_residues
MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSD
TTTNISSSQEQKTEPEKQNLQTRAEEQSVDTKATASTPSSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTLGKSETPEIEKSQIKNISEQSIKEIEKPYEELKSEAISINNLE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKP
LNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDTSGE
NLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL
>Mature_543_residues
TKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDT
TTNISSSQEQKTEPEKQNLQTRAEEQSVDTKATASTPSSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEGD
KATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTLGKSETPEIEKSQIKNISEQSIKEIEKPYEELKSEAISINNLEI
AESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKPL
NKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDTSGEN
LIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVNS
PEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=445, Percent_Identity=31.9101123595506, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=35.3982300884956, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI31711992, Length=295, Percent_Identity=29.8305084745763, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI203098816, Length=310, Percent_Identity=27.741935483871, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI203098753, Length=310, Percent_Identity=27.741935483871, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=34.6938775510204, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1786305, Length=548, Percent_Identity=45.0729927007299, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=29.1764705882353, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=416, Percent_Identity=30.5288461538462, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=228, Percent_Identity=34.6491228070175, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=26.9141531322506, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=28.2467532467532, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=400, Percent_Identity=30.5, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=343, Percent_Identity=27.6967930029155, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=32.6139088729017, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24645909, Length=211, Percent_Identity=37.4407582938389, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24582497, Length=234, Percent_Identity=29.0598290598291, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20129315, Length=234, Percent_Identity=29.0598290598291, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 59314; Mature: 59182

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQI
CCCCCCEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHH
LVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKTEPEKQNLQTRAEEQSVDTKATASTPSS
HHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
KDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEGDKATMDIPSPYAGKVIEMKI
CCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEE
KLGDKVSQGTPILTLKTLGKSETPEIEKSQIKNISEQSIKEIEKPYEELKSEAISINNLE
EECCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCEE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGF
EECCCEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCC
SLPSNPAIDFSKFGSIETKPLNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSE
CCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
SESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDTSGENLIYKKYYNIGIAVDTPNGL
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCE
VVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCEECCCCC
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIR
CCCEEEEECCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHH
RVLL
HHHC
>Mature Secondary Structure 
TKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEKASMDIPSPISGTVTQI
CCCCCEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHH
LVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKTEPEKQNLQTRAEEQSVDTKATASTPSS
HHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCC
KDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEGDKATMDIPSPYAGKVIEMKI
CCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEE
KLGDKVSQGTPILTLKTLGKSETPEIEKSQIKNISEQSIKEIEKPYEELKSEAISINNLE
EECCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCEE
IAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKSRITKEDLQNYIKVRLSEKTTSGGF
EECCCEEEEECCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHEEEECCCCCCCCC
SLPSNPAIDFSKFGSIETKPLNKIKKLTGTNVHRSWITIPHVTQFDEADITDLEAFRKSE
CCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
SESAKNQDYKLTLLAFVCSVVCKALHAYPQFNASLDTSGENLIYKKYYNIGIAVDTPNGL
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCE
VVPVIKNVDKLSVIDIAKEMSRLSTKAREKGLTPIDMSGGCFTISSLGGIGGTAFTPIVN
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCEECCCCC
SPEVAILGLSRSIIKPIYDNKEFKPRLMLPISLSYDHRVIDGAEAARFTRFLCDCLGDIR
CCCEEEEECCHHHHHHHHCCCCCCCEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHH
RVLL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]