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The map label for this gene is lpdA

Identifier: 52841732

GI number: 52841732

Start: 1660524

End: 1661963

Strand: Reverse

Name: lpdA

Synonym: lpg1502

Alternate gene names: 52841732

Gene position: 1661963-1660524 (Counterclockwise)

Preceding gene: 161723253

Following gene: 52841731

Centisome position: 48.91

GC content: 40.28

Gene sequence:

>1440_bases
TTGATGAGGCTTCTTATGGCTAATAAAATTAAGACAGATGTAGTGGTATTAGGTAGTGGACCAGGCGGATACACAGCCGC
TTTCAGGGCTGCCGACTTGGATAAAAAGGTGGTTTTGGTCGAGCGTTTTGACTCACTAGGTGGCGTTTGTTTAAATGTTG
GTTGCATTCCCTCAAAAGCATTATTGCATATTGCTAAAGTAGTAGATGAAGCCCATGAAATGTCAGAGCAAGGTGTAGCA
TTTGGTAAACCTAAATTCGACAATAAAAAATTGGTTGCCTGGAAAAACTCTGTCGTTGCAAAGTTGACCGGGGGACTAAA
AGCTCTTGCCAAACAAAGAAAAGTAGAAGTAATTACTGGGACAGGTAAGTTTTCAGGTACCCATCAAATTCTTGTTGAAA
CGAAAGAAGGGACTGTAGAAATTGAATTTGATAATGCCATTATTGCAGTAGGCTCAGAATCCATTAAATTACCATTTATA
CCAGAAGATAAACGAATATTTAGCTCTACAGGGGCATTAGAACTCGCTGATATCAAAGGTGACTTACTTGTATTGGGTGG
TGGTATTATTGGCTTGGAAATGGCAACTGTCTACTCCTCGCTGGGAGTTAATGTAACAGTTGTTGAATTTATGGACCAAT
TAATTCCGAATGCTGATGCCGACTTGGTCAATATACTGCAAAAACGAATGACTAAAAAGGGTGTCAAATTTCTATTAAAA
ACGAAAGTAACTGCAGTTGAAGCCAAAAAAGATGGTATTTATGTTTCAATGGAAGGAGAACATGCAACTGATAAGCCTCT
TTGCTTTCAGCAAGTACTTGTTTCAGTAGGCAGGAAACCTAACGGTGGAGCAATCAACGCTGAAAAAGCAGGGATCAAAG
TAGATGAACGTGGCTTTATTCCAGTTGATAATCAATTAAGAACGAATGTTCCTCATATTTTTGCTATTGGCGATGTCAAT
GGGCAACCAATGCTCGCACATAAAGCAATCCCTGAAGGGAAAGTTGCAGCAGAAGTCATTGCAGGGAAAAAACATTATTT
TGAGCCAAAATGTATACCTAGTGTTGCTTATACAGATCCAGAATTAGCTTGGGCTGGTCTAACAGAAAAAGAAGCAAAAG
AGAAAGGAATAAACTATGAGAAAGCCTCCTTTCCATGGGCTGCTAGCGGTCGTGCACTCAGCATGGGGCGAGAAGAGGGA
ATGACCAAATTACTATTCTGCCCAGAAACTAACCGTATCCTGGGAGCAGGTATTGTTGGGGTTAATGCCGGCGATTTAAT
TGCAGAAACTGCCTTAGCCATTGAAATGTGTTGTGATGTTGAAGACATCGCTCTTACTATTCATCCTCACCCGACTCTAT
CAGAAACCATAGCTCAGTCAGCAGAAGCATTTGAAGGCACTATAACGGATCTGTATTTGCCTAAAAAGAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GGTGATATTAGAAGAGTTTTACTCTGACATATAAAGGAATAGAGCTATTGGTATTTCCTTGTTCTGAATGAAATGCCAAT
AACCCCAGAAAACATATTTT

Downstream 100 bases:

>100_bases
TAATTACAGGTAGCCAATTATACCTCATTGTATTTCCTTTGCTCATAAAAGCAAAGGGATGCTTATTATATGTATATAAT
TATATATAGACCTCTTGCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MMRLLMANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGVCLNVGCIPSKALLHIAKVVDEAHEMSEQGVA
FGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFI
PEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLVNILQKRMTKKGVKFLLK
TKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVSVGRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVN
GQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEG
MTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK

Sequences:

>Translated_479_residues
MMRLLMANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGVCLNVGCIPSKALLHIAKVVDEAHEMSEQGVA
FGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFI
PEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLVNILQKRMTKKGVKFLLK
TKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVSVGRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVN
GQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEG
MTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK
>Mature_479_residues
MMRLLMANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGVCLNVGCIPSKALLHIAKVVDEAHEMSEQGVA
FGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFI
PEDKRIFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLVNILQKRMTKKGVKFLLK
TKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVSVGRKPNGGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVN
GQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEG
MTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=41.6309012875536, Blast_Score=342, Evalue=6e-94,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=27.0562770562771, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=430, Percent_Identity=28.1395348837209, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI33519428, Length=430, Percent_Identity=28.1395348837209, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI33519426, Length=430, Percent_Identity=28.1395348837209, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI148277065, Length=432, Percent_Identity=28.0092592592593, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI148277071, Length=430, Percent_Identity=28.1395348837209, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=27.437641723356, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI22035672, Length=463, Percent_Identity=26.9978401727862, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI291045268, Length=429, Percent_Identity=25.8741258741259, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=62.7368421052632, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=28.9183222958057, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI87082354, Length=433, Percent_Identity=27.2517321016166, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=26.7281105990783, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789065, Length=262, Percent_Identity=24.0458015267176, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=41.8655097613883, Blast_Score=334, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=443, Percent_Identity=26.6365688487585, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=443, Percent_Identity=26.6365688487585, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=443, Percent_Identity=25.5079006772009, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=223, Percent_Identity=27.3542600896861, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=42.5847457627119, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=29.7872340425532, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=27.3109243697479, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=42.8571428571429, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24640553, Length=472, Percent_Identity=29.8728813559322, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24640549, Length=472, Percent_Identity=29.8728813559322, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24640551, Length=472, Percent_Identity=29.8728813559322, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17737741, Length=486, Percent_Identity=27.5720164609054, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51391; Mature: 51391

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLLMANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGVCLNVGCIPSKA
CEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEEEHHCCCCCEEEEECCCCHHH
LLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVEVITG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEE
TGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKRIFSSTGALELADIKG
CCCCCCCEEEEEEECCCEEEEEECCEEEEECCCCEEECCCCCCCHHHCCCCCEEEEECCC
DLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLVNILQKRMTKKGVKFLLK
CEEEEECCEEEHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVSVGRKPNGGAINAEKAGIKVDERGFI
HHEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCEEECCCCCC
PVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVAYTDP
CCCCCHHCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCCC
ELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEGMTKLLFCPETNRILGAGIVG
CCEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCHHCCCEEEEECCCCCCEEEEEEEE
VNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK
CCCCHHHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MMRLLMANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGVCLNVGCIPSKA
CEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEEEHHCCCCCEEEEECCCCHHH
LLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLKALAKQRKVEVITG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEE
TGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKRIFSSTGALELADIKG
CCCCCCCEEEEEEECCCEEEEEECCEEEEECCCCEEECCCCCCCHHHCCCCCEEEEECCC
DLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLVNILQKRMTKKGVKFLLK
CEEEEECCEEEHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVSVGRKPNGGAINAEKAGIKVDERGFI
HHEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCEEECCCCCC
PVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEVIAGKKHYFEPKCIPSVAYTDP
CCCCCHHCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCCC
ELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGREEGMTKLLFCPETNRILGAGIVG
CCEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCHHCCCEEEEECCCCCCEEEEEEEE
VNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSETIAQSAEAFEGTITDLYLPKKKKK
CCCCHHHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]