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The map label for this gene is trmE

Identifier: 39998554

GI number: 39998554

Start: 3810131

End: 3811501

Strand: Reverse

Name: trmE

Synonym: GSU3465

Alternate gene names: 39998554

Gene position: 3811501-3810131 (Counterclockwise)

Preceding gene: 39998555

Following gene: 39998553

Centisome position: 99.93

GC content: 58.72

Gene sequence:

>1371_bases
ATGTACGTGGAAGATACCATAGCCGCCATCAGCACGGCGGCAGGGGAGGGTGGGGTCGGGATCGTCCGCGTTAGCGGACC
GGACGCCCCCTCGATTGCCCGCCGCGTTTTCAGGCGCGGTTCCAACGGTGACTTCGAAAGTCACCGTTTTTATTATGGGT
CGGTTGTTGATGCTGTCACTGGCGAGGCCGTGGACGAGGCAATGGCGGTCCTCATGGTACGCCCACGGTCCTACACCCGT
GAAGACGTCCTGGAGATCCAGTGCCACGGGGGATATCTCGTCACCCGACGCGTCCTGGAACTCGTGCTTGCCGCCGGGGC
GCGTCTCGCAGAACCCGGTGAGTTTACCCGTCGAGCCTTTCTCAATGGCCGCATCGATCTGGTTCAGGCAGAGGCTGTCA
TCGACGTGATCAGGGCCAAGACCGACGCTGCCCTTGCCTTGGCTCAGCACCAGCGCCAGGGTCGGCTATCCCAACGGCTC
GACACGGTTATGGCCGAGTTGCGTCAGGCTCTAGCACTGGTAGAAGCGTTCATCGACTTTCCCGAAGACGACATCGACCC
AGCTGCTCAGGATGCATTAACGGTCCATGTGAGGAAAGCCGCCGAAACGGTTGGAGAACTAATTGCCGGATTTGACGAGG
GACGGGTCCTGCGAGAAGGGGTGGCTGTCCTTATCGCAGGCAAACCGAACGTGGGAAAGTCTAGTCTTCTGAATACCCTT
CTTCAGGAAAAGCGGGCCATCGTCACCTCGGTACCCGGCACCACCCGAGACATTATCGAGGAGGTGGTCAATATCCGCGG
TCTGCCGCTCCGGATGATCGATACCGCCGGTATTCGCGACACTGAAGACATTGTGGAAAAAGAAGGGGTCCGCCTCACCC
TTGAAAAGATCCCCGAGGCGGATCTGGTACTGCTGGTGATCGACGGTTCGCGACCTCTTGATGAGGACGACCGGATGATC
CTTTCTGCCCTTGCCGGCAAGCGACTCATTCTCGTGGAGAACAAATGCGATCTGCCGCGGGCCGTGCAGATTCCCGATGA
ACTTGTCCTGATGCCTAGGGTCACGGTTTCCACCTCGCGAGGCGACGGCATTGATGAGCTGAAGGAGTCAATTTTCCAGA
CCTTTATTCACGGAGCTGCTATTGACAGTCGTGAGTATGTTGCTGTCTCCCGGGTTCGTCACCGCGATCTGCTGTCCCGT
TCAACGATGCACTTGACCGCGTTCGAGCAGGGACTTGCGTCGGGGTTCACTCTTGAGTTGCTGGCTGTTGAGTTGCGCGA
CGCTCTGGCCGCTGTCGGTGAGGTCACGGGTGAGACAACCCCGGACGACATCCTGGACGTCATTTTTGACCGATTCTGCA
TTGGCAAGTGA

Upstream 100 bases:

>100_bases
GGGCCTTGTGCTTTACTGGCTCGTCAACAACGTGCTTACCATCATTCAGCAGTACTACATCAACCGCAGCATCAGTACGG
CGGAGGCCAAGTAGCGGACC

Downstream 100 bases:

>100_bases
CCCGAAACGCCACGTGAGGGGCATGGCTGACGCGGGTTCCCCGTGCCACACGGTGTAGGGGTCAGCTCAAAATTGGTCCG
ATGTCGGCATCATGTTTCAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MYVEDTIAAISTAAGEGGVGIVRVSGPDAPSIARRVFRRGSNGDFESHRFYYGSVVDAVTGEAVDEAMAVLMVRPRSYTR
EDVLEIQCHGGYLVTRRVLELVLAAGARLAEPGEFTRRAFLNGRIDLVQAEAVIDVIRAKTDAALALAQHQRQGRLSQRL
DTVMAELRQALALVEAFIDFPEDDIDPAAQDALTVHVRKAAETVGELIAGFDEGRVLREGVAVLIAGKPNVGKSSLLNTL
LQEKRAIVTSVPGTTRDIIEEVVNIRGLPLRMIDTAGIRDTEDIVEKEGVRLTLEKIPEADLVLLVIDGSRPLDEDDRMI
LSALAGKRLILVENKCDLPRAVQIPDELVLMPRVTVSTSRGDGIDELKESIFQTFIHGAAIDSREYVAVSRVRHRDLLSR
STMHLTAFEQGLASGFTLELLAVELRDALAAVGEVTGETTPDDILDVIFDRFCIGK

Sequences:

>Translated_456_residues
MYVEDTIAAISTAAGEGGVGIVRVSGPDAPSIARRVFRRGSNGDFESHRFYYGSVVDAVTGEAVDEAMAVLMVRPRSYTR
EDVLEIQCHGGYLVTRRVLELVLAAGARLAEPGEFTRRAFLNGRIDLVQAEAVIDVIRAKTDAALALAQHQRQGRLSQRL
DTVMAELRQALALVEAFIDFPEDDIDPAAQDALTVHVRKAAETVGELIAGFDEGRVLREGVAVLIAGKPNVGKSSLLNTL
LQEKRAIVTSVPGTTRDIIEEVVNIRGLPLRMIDTAGIRDTEDIVEKEGVRLTLEKIPEADLVLLVIDGSRPLDEDDRMI
LSALAGKRLILVENKCDLPRAVQIPDELVLMPRVTVSTSRGDGIDELKESIFQTFIHGAAIDSREYVAVSRVRHRDLLSR
STMHLTAFEQGLASGFTLELLAVELRDALAAVGEVTGETTPDDILDVIFDRFCIGK
>Mature_456_residues
MYVEDTIAAISTAAGEGGVGIVRVSGPDAPSIARRVFRRGSNGDFESHRFYYGSVVDAVTGEAVDEAMAVLMVRPRSYTR
EDVLEIQCHGGYLVTRRVLELVLAAGARLAEPGEFTRRAFLNGRIDLVQAEAVIDVIRAKTDAALALAQHQRQGRLSQRL
DTVMAELRQALALVEAFIDFPEDDIDPAAQDALTVHVRKAAETVGELIAGFDEGRVLREGVAVLIAGKPNVGKSSLLNTL
LQEKRAIVTSVPGTTRDIIEEVVNIRGLPLRMIDTAGIRDTEDIVEKEGVRLTLEKIPEADLVLLVIDGSRPLDEDDRMI
LSALAGKRLILVENKCDLPRAVQIPDELVLMPRVTVSTSRGDGIDELKESIFQTFIHGAAIDSREYVAVSRVRHRDLLSR
STMHLTAFEQGLASGFTLELLAVELRDALAAVGEVTGETTPDDILDVIFDRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=34.4827586206897, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI193082993, Length=453, Percent_Identity=34.4370860927152, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=32.741617357002, Blast_Score=208, Evalue=8e-54,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=37.5, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082120, Length=170, Percent_Identity=36.4705882352941, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=29.7180043383948, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=28.8888888888889, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=32.1353065539112, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_GEOSL (Q746Q3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954505.1
- HSSP:   Q9X1F8
- ProteinModelPortal:   Q746Q3
- SMR:   Q746Q3
- GeneID:   2686588
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3465
- NMPDR:   fig|243231.1.peg.3443
- TIGR:   GSU3465
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   GSUL243231:GSU_3465-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49709; Mature: 49709

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 85-85 BINDING 124-124 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVEDTIAAISTAAGEGGVGIVRVSGPDAPSIARRVFRRGSNGDFESHRFYYGSVVDAVT
CCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHH
GEAVDEAMAVLMVRPRSYTREDVLEIQCHGGYLVTRRVLELVLAAGARLAEPGEFTRRAF
HHHHHHHHHHHEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNGRIDLVQAEAVIDVIRAKTDAALALAQHQRQGRLSQRLDTVMAELRQALALVEAFIDF
HCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDDIDPAAQDALTVHVRKAAETVGELIAGFDEGRVLREGVAVLIAGKPNVGKSSLLNTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCHHHHHHHH
LQEKRAIVTSVPGTTRDIIEEVVNIRGLPLRMIDTAGIRDTEDIVEKEGVRLTLEKIPEA
HHHHHHEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCEEEHHHCCCC
DLVLLVIDGSRPLDEDDRMILSALAGKRLILVENKCDLPRAVQIPDELVLMPRVTVSTSR
CEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCEEEECCEEEECCC
GDGIDELKESIFQTFIHGAAIDSREYVAVSRVRHRDLLSRSTMHLTAFEQGLASGFTLEL
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHH
LAVELRDALAAVGEVTGETTPDDILDVIFDRFCIGK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYVEDTIAAISTAAGEGGVGIVRVSGPDAPSIARRVFRRGSNGDFESHRFYYGSVVDAVT
CCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHH
GEAVDEAMAVLMVRPRSYTREDVLEIQCHGGYLVTRRVLELVLAAGARLAEPGEFTRRAF
HHHHHHHHHHHEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNGRIDLVQAEAVIDVIRAKTDAALALAQHQRQGRLSQRLDTVMAELRQALALVEAFIDF
HCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDDIDPAAQDALTVHVRKAAETVGELIAGFDEGRVLREGVAVLIAGKPNVGKSSLLNTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCHHHHHHHH
LQEKRAIVTSVPGTTRDIIEEVVNIRGLPLRMIDTAGIRDTEDIVEKEGVRLTLEKIPEA
HHHHHHEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCEEEHHHCCCC
DLVLLVIDGSRPLDEDDRMILSALAGKRLILVENKCDLPRAVQIPDELVLMPRVTVSTSR
CEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCEEEECCEEEECCC
GDGIDELKESIFQTFIHGAAIDSREYVAVSRVRHRDLLSRSTMHLTAFEQGLASGFTLEL
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHH
LAVELRDALAAVGEVTGETTPDDILDVIFDRFCIGK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA