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The map label for this gene is gidA

Identifier: 39998553

GI number: 39998553

Start: 3808041

End: 3809924

Strand: Reverse

Name: gidA

Synonym: GSU3464

Alternate gene names: 39998553

Gene position: 3809924-3808041 (Counterclockwise)

Preceding gene: 39998554

Following gene: 39998552

Centisome position: 99.89

GC content: 61.62

Gene sequence:

>1884_bases
ATGAATCTCATTGATTACGAAAAGCAGTACGACGTCATCGTGGCGGGAGCCGGCCATGCGGGCTGCGAGGCGGCCCTGGC
CGCGGCGCGGATGGGATGCGAGACCCTGCTTCTCACCATCAACCTGGATGCCATCGCCCTCATGTCGTGCAATCCGGCCA
TCGGCGGACTCGCCAAGGGGCACTTGGTGAAGGAGATCGATGCGCTGGGCGGCGAGATGGGAAAGAACATCGATGCCACC
GGCATCCAGTACCGCATTCTCAATACCCGCAAGGGGCCTGCGGTACGGGCCTCCCGCGCCCAGGCTGACAAGCAGCTCTA
TCGCCTCCGGATGAAGCACGTAATGGAGGAACAGGAACATCTCTCGCTCAAGCAGGGTGAGGTCACGGGCCTCGTGGTTG
AAGATGGTCGGGTGCGGGGAGTGGTGACCAAGGTCGGGGTTCGCTTTCTGGGTAAAACGGTTATTCTCACCACCGGGACC
TTCATGCGCGGACTGATCCACGTGGGTCTCACCAACTACCCGGGCGGCAGGGCGGGAGACCTGCCTTCGGTGGGGCTGTC
TGACCAGTTGCGCGATCTCGGCTTCACGGTCGGCCGCCTCAAGACGGGAACCCCGGCGCGCCTGGACGGGAACACCATCG
ATTTTTCCCGGTTGGAACCCCAGTACGGAGATGATCCGCCAGTGCCGTTTTCCTTTTCTACCGAACGGATCGATCGGCCC
CAGCTTCCCTGCTACATTGCTTACACCAACGAACGTACCCACGAAATCATCCGGAGCGGTCTTGACCGTTCACCTCTCTA
TTCGGGCGTTATTGAAGGGGTTGGTCCCCGGTATTGCCCGTCCATCGAGGACAAGGTCATGCGGTTCCCGGACAAGGACC
GCCACCAGAGTTTTCTTGAGCCAGAGGGGCGCGATACGGTGGAATACTATCCGTCGGGCCTCTCCACCTCTCTTCCCATC
GATATCCAGTATCGCCTCTACCGCTCCATCGAGGGGCTGGAAAACGTTGAGATCATGCGGCCGGCCTACGCCATCGAGTA
CGACTACGTGGATCCGATTCAACTCCATACGTCACTTGAAACAAAGCTCATCCGCAACCTCTATCACGCCGGCCAGATCA
ACGGCACATCCGGCTACGAGGAGGCGGCGGGGCAGGGGCTCATGGCGGGCATCAACGCGGCGTTGCGAGTGCAGGGTGAG
GAGCCCCTGGTGCTCGGCCGCGACGAGGCCTACATCGGGGTCATGATCGACGATCTGGTAACGTTGGGGACCCGCGAACC
CTACCGGATGTTCACGTCGCGGGCCGAATACCGGTTGCTGCTGCGGGAGGACAACGCCGACCTGCGCCTTCGGGAACGTG
GCCACGCTGTTGGTCTCGTTCGGGACGAGGAGTACCGGCTGTTCCTGGAAAAACGTGAGCGGATCGGTGCGGAGCTGGAA
CGGTTGCGCACCGCGAAACTCCTGCCGTCCGAGGCGGATCCGTCGTTCCTCGAAACCTACGGCATGACCGATCTGCGCAA
TGCTTTGACGTTTGAGCAGCTCTTGCGGCGGCCCGACATCACCTATGAAGAGCTTTCGCAGATTGACCCGGTTGCGGGTA
TGGTTCCGCCGTCGGTGAAGGAACAGGTGGAGATCCAGATCAAATATCAGGGCTACATCGAGCGGCAGCTGGACCAGGTG
GCGCGGGCGCGCAAGCTGGAAGGGACCCGTATCCCCGACGACCTGGACTACACCGTCATCCCCGGTCTCTCCGCCGAGGT
GAGGGAAAAGCTCCTTCGCTTTCTCCCCGATACCTTGGGGCAGGCCTCACGCATCCAGGGGGTCACCCCTGCCGCCGTCG
GTATCCTTTCCGTGGCCATCAAATCCAGGAGCGCGTCGTCGTGA

Upstream 100 bases:

>100_bases
CGTTTGCTGTAACATTGCGAATGTAGTGATAAAAAAGCTGGGATACAGTATCCTTGAAGCTCTGATGTGAAACGAAGGGG
AAAGGAGGCGGCGGGACGTC

Downstream 100 bases:

>100_bases
ACGGCCGGGCCTGCCGGATACTGCAGGAAGGTGCTGCCGAGCTGGGTGTCGAGATCAGTGACGAACTGGTCACGTTATTT
TCCCTCTTTGCCGACGAGCT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MNLIDYEKQYDVIVAGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGLAKGHLVKEIDALGGEMGKNIDAT
GIQYRILNTRKGPAVRASRAQADKQLYRLRMKHVMEEQEHLSLKQGEVTGLVVEDGRVRGVVTKVGVRFLGKTVILTTGT
FMRGLIHVGLTNYPGGRAGDLPSVGLSDQLRDLGFTVGRLKTGTPARLDGNTIDFSRLEPQYGDDPPVPFSFSTERIDRP
QLPCYIAYTNERTHEIIRSGLDRSPLYSGVIEGVGPRYCPSIEDKVMRFPDKDRHQSFLEPEGRDTVEYYPSGLSTSLPI
DIQYRLYRSIEGLENVEIMRPAYAIEYDYVDPIQLHTSLETKLIRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVQGE
EPLVLGRDEAYIGVMIDDLVTLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVRDEEYRLFLEKRERIGAELE
RLRTAKLLPSEADPSFLETYGMTDLRNALTFEQLLRRPDITYEELSQIDPVAGMVPPSVKEQVEIQIKYQGYIERQLDQV
ARARKLEGTRIPDDLDYTVIPGLSAEVREKLLRFLPDTLGQASRIQGVTPAAVGILSVAIKSRSASS

Sequences:

>Translated_627_residues
MNLIDYEKQYDVIVAGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGLAKGHLVKEIDALGGEMGKNIDAT
GIQYRILNTRKGPAVRASRAQADKQLYRLRMKHVMEEQEHLSLKQGEVTGLVVEDGRVRGVVTKVGVRFLGKTVILTTGT
FMRGLIHVGLTNYPGGRAGDLPSVGLSDQLRDLGFTVGRLKTGTPARLDGNTIDFSRLEPQYGDDPPVPFSFSTERIDRP
QLPCYIAYTNERTHEIIRSGLDRSPLYSGVIEGVGPRYCPSIEDKVMRFPDKDRHQSFLEPEGRDTVEYYPSGLSTSLPI
DIQYRLYRSIEGLENVEIMRPAYAIEYDYVDPIQLHTSLETKLIRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVQGE
EPLVLGRDEAYIGVMIDDLVTLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVRDEEYRLFLEKRERIGAELE
RLRTAKLLPSEADPSFLETYGMTDLRNALTFEQLLRRPDITYEELSQIDPVAGMVPPSVKEQVEIQIKYQGYIERQLDQV
ARARKLEGTRIPDDLDYTVIPGLSAEVREKLLRFLPDTLGQASRIQGVTPAAVGILSVAIKSRSASS
>Mature_627_residues
MNLIDYEKQYDVIVAGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGLAKGHLVKEIDALGGEMGKNIDAT
GIQYRILNTRKGPAVRASRAQADKQLYRLRMKHVMEEQEHLSLKQGEVTGLVVEDGRVRGVVTKVGVRFLGKTVILTTGT
FMRGLIHVGLTNYPGGRAGDLPSVGLSDQLRDLGFTVGRLKTGTPARLDGNTIDFSRLEPQYGDDPPVPFSFSTERIDRP
QLPCYIAYTNERTHEIIRSGLDRSPLYSGVIEGVGPRYCPSIEDKVMRFPDKDRHQSFLEPEGRDTVEYYPSGLSTSLPI
DIQYRLYRSIEGLENVEIMRPAYAIEYDYVDPIQLHTSLETKLIRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVQGE
EPLVLGRDEAYIGVMIDDLVTLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVRDEEYRLFLEKRERIGAELE
RLRTAKLLPSEADPSFLETYGMTDLRNALTFEQLLRRPDITYEELSQIDPVAGMVPPSVKEQVEIQIKYQGYIERQLDQV
ARARKLEGTRIPDDLDYTVIPGLSAEVREKLLRFLPDTLGQASRIQGVTPAAVGILSVAIKSRSASS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=629, Percent_Identity=46.1049284578696, Blast_Score=504, Evalue=1e-142,
Organism=Homo sapiens, GI19882217, Length=654, Percent_Identity=44.3425076452599, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI183227703, Length=669, Percent_Identity=43.3482810164424, Blast_Score=484, Evalue=1e-137,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=56.9354838709677, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=633, Percent_Identity=41.0742496050553, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321202, Length=633, Percent_Identity=44.5497630331754, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24658174, Length=628, Percent_Identity=43.6305732484076, Blast_Score=473, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_GEOSL (Q746Q4)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954504.1
- ProteinModelPortal:   Q746Q4
- SMR:   Q746Q4
- GeneID:   2686579
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3464
- NMPDR:   fig|243231.1.peg.3442
- TIGR:   GSU3464
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   GSUL243231:GSU_3464-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69729; Mature: 69729

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 128-128 BINDING 183-183 BINDING 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIDYEKQYDVIVAGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGLAKG
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCCCCHHH
HLVKEIDALGGEMGKNIDATGIQYRILNTRKGPAVRASRAQADKQLYRLRMKHVMEEQEH
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLKQGEVTGLVVEDGRVRGVVTKVGVRFLGKTVILTTGTFMRGLIHVGLTNYPGGRAGD
CCCCCCCEEEEEEECCCEEHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCC
LPSVGLSDQLRDLGFTVGRLKTGTPARLDGNTIDFSRLEPQYGDDPPVPFSFSTERIDRP
CCCCCCHHHHHHHCCCHHCCCCCCCCEECCCCCCHHHCCCCCCCCCCCCCCCCHHHCCCC
QLPCYIAYTNERTHEIIRSGLDRSPLYSGVIEGVGPRYCPSIEDKVMRFPDKDRHQSFLE
CCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHCC
PEGRDTVEYYPSGLSTSLPIDIQYRLYRSIEGLENVEIMRPAYAIEYDYVDPIQLHTSLE
CCCCCCHHCCCCCCCCCCCEEEHHHHHHHHCCCCCCEEECCEEEEEECCCCCHHHHHHHH
TKLIRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVQGEEPLVLGRDEAYIGVMIDDLV
HHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCEEEECCCCEEEEEHHHHH
TLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVRDEEYRLFLEKRERIGAELE
HHCCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHH
RLRTAKLLPSEADPSFLETYGMTDLRNALTFEQLLRRPDITYEELSQIDPVAGMVPPSVK
HHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCC
EQVEIQIKYQGYIERQLDQVARARKLEGTRIPDDLDYTVIPGLSAEVREKLLRFLPDTLG
CEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
QASRIQGVTPAAVGILSVAIKSRSASS
CHHHCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLIDYEKQYDVIVAGAGHAGCEAALAAARMGCETLLLTINLDAIALMSCNPAIGGLAKG
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCCCCHHH
HLVKEIDALGGEMGKNIDATGIQYRILNTRKGPAVRASRAQADKQLYRLRMKHVMEEQEH
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLKQGEVTGLVVEDGRVRGVVTKVGVRFLGKTVILTTGTFMRGLIHVGLTNYPGGRAGD
CCCCCCCEEEEEEECCCEEHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCC
LPSVGLSDQLRDLGFTVGRLKTGTPARLDGNTIDFSRLEPQYGDDPPVPFSFSTERIDRP
CCCCCCHHHHHHHCCCHHCCCCCCCCEECCCCCCHHHCCCCCCCCCCCCCCCCHHHCCCC
QLPCYIAYTNERTHEIIRSGLDRSPLYSGVIEGVGPRYCPSIEDKVMRFPDKDRHQSFLE
CCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHCC
PEGRDTVEYYPSGLSTSLPIDIQYRLYRSIEGLENVEIMRPAYAIEYDYVDPIQLHTSLE
CCCCCCHHCCCCCCCCCCCEEEHHHHHHHHCCCCCCEEECCEEEEEECCCCCHHHHHHHH
TKLIRNLYHAGQINGTSGYEEAAGQGLMAGINAALRVQGEEPLVLGRDEAYIGVMIDDLV
HHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCEEEECCCCEEEEEHHHHH
TLGTREPYRMFTSRAEYRLLLREDNADLRLRERGHAVGLVRDEEYRLFLEKRERIGAELE
HHCCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHH
RLRTAKLLPSEADPSFLETYGMTDLRNALTFEQLLRRPDITYEELSQIDPVAGMVPPSVK
HHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCC
EQVEIQIKYQGYIERQLDQVARARKLEGTRIPDDLDYTVIPGLSAEVREKLLRFLPDTLG
CEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
QASRIQGVTPAAVGILSVAIKSRSASS
CHHHCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA