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The map label for this gene is def-2 [H]

Identifier: 39998545

GI number: 39998545

Start: 3803386

End: 3803895

Strand: Reverse

Name: def-2 [H]

Synonym: GSU3456

Alternate gene names: 39998545

Gene position: 3803895-3803386 (Counterclockwise)

Preceding gene: 39998546

Following gene: 308513345

Centisome position: 99.73

GC content: 62.35

Gene sequence:

>510_bases
ATGCCAGCACAGACCATACTGCAGTATCCCCATCCCGTCCTGAAGAAGGTATGTCATACTGTCACGGCCATCGACGAGGC
CATCAGGGGGCTGATCGACGACCTGATCGAGACCATGCGTGAAGGTCCCGGCTCCGTGGGCGTTGCCGCCCCCCAGATCG
GCGTCACCTTGCGGGTCTGCGTCATTGACGTCTCGGGCAGCCGCCACGGCAAGGACAACAACCACGGGCTGCTCCTCATG
GTCAACCCGGAGATCGTTGACCGGTCGGGCAATGCGGTCATGCGCGAAGGATGCATGAGCGTCCCCGACTACACGGGAGA
CGTGGAGCGCTCCACCGAGGTCCGGGTCCGCTTCCTGGACGGCGCGGATGGCTCGGAGCGGGAGATCACGGCCTCCGGCT
TCGAGGCCGTCGCAATCCAGCACGAGATGGACCACTTGGACGGCATCCTGTTCCTCGACCGGATTGTCTCGATCAAAACG
GGCCTCTTCCGGCGGAAAAATTATAAGTAA

Upstream 100 bases:

>100_bases
ATCTCGGTGGACCAGGTGTCGGCCATGCTCGAAGAAATCAGGAAGGAGCTGAACGTGGAGATCTCGGTGCGCTCAATCAC
ACCGGTTTCCCTCTGACGCC

Downstream 100 bases:

>100_bases
CGGCCAGCCAGGCTACGGACCGCCATGGCGGCTGAACGCGCAGCAGCGTCGGCATTCCGCCGGTCCGGGGGTGATCTGCG
CCGCAGGAGGCGGGGAACCG

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MPAQTILQYPHPVLKKVCHTVTAIDEAIRGLIDDLIETMREGPGSVGVAAPQIGVTLRVCVIDVSGSRHGKDNNHGLLLM
VNPEIVDRSGNAVMREGCMSVPDYTGDVERSTEVRVRFLDGADGSEREITASGFEAVAIQHEMDHLDGILFLDRIVSIKT
GLFRRKNYK

Sequences:

>Translated_169_residues
MPAQTILQYPHPVLKKVCHTVTAIDEAIRGLIDDLIETMREGPGSVGVAAPQIGVTLRVCVIDVSGSRHGKDNNHGLLLM
VNPEIVDRSGNAVMREGCMSVPDYTGDVERSTEVRVRFLDGADGSEREITASGFEAVAIQHEMDHLDGILFLDRIVSIKT
GLFRRKNYK
>Mature_168_residues
PAQTILQYPHPVLKKVCHTVTAIDEAIRGLIDDLIETMREGPGSVGVAAPQIGVTLRVCVIDVSGSRHGKDNNHGLLLMV
NPEIVDRSGNAVMREGCMSVPDYTGDVERSTEVRVRFLDGADGSEREITASGFEAVAIQHEMDHLDGILFLDRIVSIKTG
LFRRKNYK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=37.8698224852071, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=32.8571428571429, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18539; Mature: 18407

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQTILQYPHPVLKKVCHTVTAIDEAIRGLIDDLIETMREGPGSVGVAAPQIGVTLRVC
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
VIDVSGSRHGKDNNHGLLLMVNPEIVDRSGNAVMREGCMSVPDYTGDVERSTEVRVRFLD
EEEECCCCCCCCCCCEEEEEECCHHCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
GADGSEREITASGFEAVAIQHEMDHLDGILFLDRIVSIKTGLFRRKNYK
CCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAQTILQYPHPVLKKVCHTVTAIDEAIRGLIDDLIETMREGPGSVGVAAPQIGVTLRVC
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
VIDVSGSRHGKDNNHGLLLMVNPEIVDRSGNAVMREGCMSVPDYTGDVERSTEVRVRFLD
EEEECCCCCCCCCCCEEEEEECCHHCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
GADGSEREITASGFEAVAIQHEMDHLDGILFLDRIVSIKTGLFRRKNYK
CCCCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA