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The map label for this gene is gatB [H]

Identifier: 39998469

GI number: 39998469

Start: 3715702

End: 3717141

Strand: Reverse

Name: gatB [H]

Synonym: GSU3380

Alternate gene names: 39998469

Gene position: 3717141-3715702 (Counterclockwise)

Preceding gene: 39998470

Following gene: 39998468

Centisome position: 97.46

GC content: 63.12

Gene sequence:

>1440_bases
ATGAACTATCAGGCCGTCATTGGTTTAGAAGTCCACGTCCAGCTGAAGACCGACACCAAGATCTTCTGCGGCTGCTCCAC
AACGTTCGGTGCTTCGCCCAACTCCCAGACCTGCCCTGTCTGCCTCGGCATGCCGGGGGTCCTGCCGGTGCTCAACAAGA
AGGTGGTGGAGTTCGCCATCAGGGCCGGCCTGGCCACCAACTGCCGGATCGCGCCCCGCTCGGTCTTCGCCCGGAAGAAC
TATTTCTACCCGGACTTGCCCAAGGGTTACCAGATCAGCCAGTACGAGCTCCCCATCTGCCAGAATGGACACCTGGACAT
CGAGGTGGATGGTCAGGTGAAGCGGATCGGCATTACCCGCATCCACATGGAGGAGGATGCCGGCAAGCTGGTGCACGCCG
ACGTCCCCGGCCTGGGGAGCGGTTCCGGCGTGGACCTGAATCGCGCCTGCACGCCGCTGCTGGAGATCGTCTCGGAACCG
GACATCCGCAGTGCCGACGAGGCTGTGGCCTATCTCAGGAAGCTCCACCAGATCGTGGTCTACCTGGGTATCTGCGATGG
CAACATGGAAGAGGGGAGCTTCCGCTGCGATGCCAACGTGTCGGTCATGCCCGTGGGCTCCACGACCTTCGGCACCCGTA
CCGAGACCAAAAACGTAAACTCGTTCCGCTTCGTGAAGCAGGCCATCGAGCACGAGATAGAGCGGCAGATCGAGCTGATC
GAAGAGGGAGGGAAGGTGGTGCAGGAGACCCGCCTCTTCGATCCGAATACCGGTGAAACTCGCTCCATGCGCGGCAAGGA
GGAGGCCCACGACTACCGCTACTTCCCCGACCCCGACCTGGTGCCGCTGGTCATCTCAAACGACTGGGTCGAGGATACGC
GCCTCTCCCTCCCGGAACTCCCCGATGCGCGGCGGAGCCGCTACCGCTCCGAGCTGGGGCTTTCCGACTACGACGCCGAG
GTCCTCACCGCTACCCGGGAGATGGCCGAGTACTTCGAAAACTGCCTTGCCGCGGGCGCCCCGGCCAAGGGGGCGGCCAA
CTGGGTGATGGGAGAGGTGACTCGCGCCCTGAACGAGGCTGGCAAGGACATCGCCGAATGCCCGGTTGCTCCGGCCCGTC
TCACGGCGCTGCTGCAACTGATCGAGAAGGGCACCATCTCCGGCAAGATCGCCAAGACCGTCTTCGATGAGATGTGGCAA
AGCGACAAGGCCCCGGAGGCCATCGTGGAGGAGAAGGGGCTCGTCCAGGTCTCCGACACCGGGGCCATCGAGAAGATCAT
CGACGAGATCATGGCCGCCAACATGGGCCAGGTTGAGGAGTTCCGCGGCGGCAAGGAGAAGGTCTTCGGCTTCTTCGTGG
GCCAGGTGATGCGGGCCTCCAAGGGGAAGGCCAACCCGGCGGTGGTGAATGAGCTGCTGATGAAGAAGCTGAAAGGCTAA

Upstream 100 bases:

>100_bases
CCCTTCGGGGAGGAGACCATCCTGCGGGCCGCCCATGCCTTTGAACAGGCAACGGAATGGCATCGGCACACAGCCCAACT
CTAGCTCTCCGGAGATTTCG

Downstream 100 bases:

>100_bases
ATACGACGAGGTTCCCATGTCCTTCAGAACCATAGAGTGGCGCGACGACAAGGTTGTCATGATCGACCAGACCCGCCTGC
CCGGCGAGGAGGTCTACTGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNYQAVIGLEVHVQLKTDTKIFCGCSTTFGASPNSQTCPVCLGMPGVLPVLNKKVVEFAIRAGLATNCRIAPRSVFARKN
YFYPDLPKGYQISQYELPICQNGHLDIEVDGQVKRIGITRIHMEEDAGKLVHADVPGLGSGSGVDLNRACTPLLEIVSEP
DIRSADEAVAYLRKLHQIVVYLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEHEIERQIELI
EEGGKVVQETRLFDPNTGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVEDTRLSLPELPDARRSRYRSELGLSDYDAE
VLTATREMAEYFENCLAAGAPAKGAANWVMGEVTRALNEAGKDIAECPVAPARLTALLQLIEKGTISGKIAKTVFDEMWQ
SDKAPEAIVEEKGLVQVSDTGAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLMKKLKG

Sequences:

>Translated_479_residues
MNYQAVIGLEVHVQLKTDTKIFCGCSTTFGASPNSQTCPVCLGMPGVLPVLNKKVVEFAIRAGLATNCRIAPRSVFARKN
YFYPDLPKGYQISQYELPICQNGHLDIEVDGQVKRIGITRIHMEEDAGKLVHADVPGLGSGSGVDLNRACTPLLEIVSEP
DIRSADEAVAYLRKLHQIVVYLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEHEIERQIELI
EEGGKVVQETRLFDPNTGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVEDTRLSLPELPDARRSRYRSELGLSDYDAE
VLTATREMAEYFENCLAAGAPAKGAANWVMGEVTRALNEAGKDIAECPVAPARLTALLQLIEKGTISGKIAKTVFDEMWQ
SDKAPEAIVEEKGLVQVSDTGAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLMKKLKG
>Mature_479_residues
MNYQAVIGLEVHVQLKTDTKIFCGCSTTFGASPNSQTCPVCLGMPGVLPVLNKKVVEFAIRAGLATNCRIAPRSVFARKN
YFYPDLPKGYQISQYELPICQNGHLDIEVDGQVKRIGITRIHMEEDAGKLVHADVPGLGSGSGVDLNRACTPLLEIVSEP
DIRSADEAVAYLRKLHQIVVYLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEHEIERQIELI
EEGGKVVQETRLFDPNTGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVEDTRLSLPELPDARRSRYRSELGLSDYDAE
VLTATREMAEYFENCLAAGAPAKGAANWVMGEVTRALNEAGKDIAECPVAPARLTALLQLIEKGTISGKIAKTVFDEMWQ
SDKAPEAIVEEKGLVQVSDTGAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLMKKLKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=38.8329979879276, Blast_Score=332, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI115534094, Length=472, Percent_Identity=31.7796610169492, Blast_Score=228, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=28.9370078740157, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=33.8056680161943, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52669; Mature: 52669

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQAVIGLEVHVQLKTDTKIFCGCSTTFGASPNSQTCPVCLGMPGVLPVLNKKVVEFAI
CCCEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
RAGLATNCRIAPRSVFARKNYFYPDLPKGYQISQYELPICQNGHLDIEVDGQVKRIGITR
HHCCCCCCEECHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCEEEEEEEE
IHMEEDAGKLVHADVPGLGSGSGVDLNRACTPLLEIVSEPDIRSADEAVAYLRKLHQIVV
EEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHE
YLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEHEIERQIELI
EEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEGGKVVQETRLFDPNTGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVEDTRLSLPEL
HHCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCHHHCCCCCC
PDARRSRYRSELGLSDYDAEVLTATREMAEYFENCLAAGAPAKGAANWVMGEVTRALNEA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GKDIAECPVAPARLTALLQLIEKGTISGKIAKTVFDEMWQSDKAPEAIVEEKGLVQVSDT
CCHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCEEECCC
GAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLMKKLKG
CHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYQAVIGLEVHVQLKTDTKIFCGCSTTFGASPNSQTCPVCLGMPGVLPVLNKKVVEFAI
CCCEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
RAGLATNCRIAPRSVFARKNYFYPDLPKGYQISQYELPICQNGHLDIEVDGQVKRIGITR
HHCCCCCCEECHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCEEEEEEEE
IHMEEDAGKLVHADVPGLGSGSGVDLNRACTPLLEIVSEPDIRSADEAVAYLRKLHQIVV
EEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHE
YLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEHEIERQIELI
EEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEGGKVVQETRLFDPNTGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVEDTRLSLPEL
HHCCHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCHHHCCCCCC
PDARRSRYRSELGLSDYDAEVLTATREMAEYFENCLAAGAPAKGAANWVMGEVTRALNEA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GKDIAECPVAPARLTALLQLIEKGTISGKIAKTVFDEMWQSDKAPEAIVEEKGLVQVSDT
CCHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCEEECCC
GAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLMKKLKG
CHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA