Click here to switch to the map view.

The map label for this gene is gatA

Identifier: 39998470

GI number: 39998470

Start: 3717158

End: 3718615

Strand: Reverse

Name: gatA

Synonym: GSU3381

Alternate gene names: 39998470

Gene position: 3718615-3717158 (Counterclockwise)

Preceding gene: 39998471

Following gene: 39998469

Centisome position: 97.5

GC content: 66.26

Gene sequence:

>1458_bases
ATGGAGCTGTTTGAGCTGACCCTGCACGAACTGCATGACAAGCTGGCGAAACGGGAGGTTTCCTCCGTGGAGGCGACCCG
GGCGCTGCTCGCCCGCATCGAGGCCACCGACTCCCGGGTGAATGCCTATATTACCGTTACTCCCGACGAGGCCCTTGCTG
CCGCCGAAGCGGCTGACCGGCGCATTGCCGCCGGCGGGCTGACCCCCCTCACCGGCGTCCCGGTTGCACTCAAGGACATC
TTCGTCACCCGCGGGATTCGCACTACCTGCGGCTCGAAGATCCTCGGGAATTTCATTCCCCCCTATGACGGAACGGTCGT
GGCGAAGCTCCGGGAGGCGGGGGCGGTCATCGTGGGCAAACTCAATCAGGACGAGTTCGCCATGGGCTCATCCGGTGAAT
CCAGCGCCTTCGGCGCCACCAAAAACCCCTGGAACCTGGCGTGCATCCCCGGCGGTTCGTCCAGCGGTTCGGCCGCGGCC
ATCGCGGCACGCTCGGCAACGGCCACGCTCGGCACCGACACCGGTGGCTCCATCCGGCAGCCCGCCTCCCACTGCGGCTG
CGTGGGGCTGCGCCCCACCTACGGCCGGGTCTCCCGCTACGGGGTCATCGCCTATGCCTCGTCCCTGGACCAGGTGGGTC
CCGTGACCCGCGATGTGACCGACTGCGCCCTCATGCTGGGAGCCGTGGCCGGTCACGACCCCCTCGACTCCACCAGCATC
GATCTGCCGGTGCCCGATTACGCCGCAGCCCTTACCGGCCAGGTGAAGGGGCTCAGGCTGGGACTGCCGAAGGAATACTA
CCTGGAAGGGCTTGACCCGGACGTGAAGCGGGCACTGGATGCCGCCATCGAGACGTACCGGGGGCTCGGCGCCGAGTTTG
TGGAGGTTTCGCTCCCTCACACCGACTATGCCGTGGCCACCTATTACCTCATCGCTACCGCCGAGGCCAGCTCCAACCTG
GCCCGCTACGACGGGGTCCGCTTCGGGCACCGGGCGGCCGGGGCCGCGAACCTGATCGACATGTTCCGTCGCAGCCGCGC
CGAGGGGTTCGGCGCCGAGGTGAAGCGCCGGATCATGATCGGCACCTACGCCCTTTCCTCGGGATATTACGACGCTTACT
ACCTGAAGGCGCAGAAGGTCCGCACCCTGATCATGCAGGACTTCATGAAAGCCTTCGAGCAGGTCGATGCCCTGCTCACG
CCGGTGGCGCCGACCCCGGCCTTCAAGATCGGCGAGAAGGTGGATGATCCGCTTCAGATGTACCTGTCGGATATCTTCAC
CATCCCTGTGAACCTTGCCGGCACCTGCGCCATTTCCGTTCCCGCCGGCATGAGCGCCGCCGGTCTCCCCATCGGCCTGC
AGCTCATCGGTCGTCCCTTCGGGGAGGAGACCATCCTGCGGGCCGCCCATGCCTTTGAACAGGCAACGGAATGGCATCGG
CACACAGCCCAACTCTAG

Upstream 100 bases:

>100_bases
CGGCGCCACCATGTACCCCATTGAAGTGGCCCCTTACCGGCTGAAGAAGAAATGACCCGGCCCGGCCAATGCCGGGCCCA
CTGACTGGAGACAGAGCGAC

Downstream 100 bases:

>100_bases
CTCTCCGGAGATTTCGATGAACTATCAGGCCGTCATTGGTTTAGAAGTCCACGTCCAGCTGAAGACCGACACCAAGATCT
TCTGCGGCTGCTCCACAACG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MELFELTLHELHDKLAKREVSSVEATRALLARIEATDSRVNAYITVTPDEALAAAEAADRRIAAGGLTPLTGVPVALKDI
FVTRGIRTTCGSKILGNFIPPYDGTVVAKLREAGAVIVGKLNQDEFAMGSSGESSAFGATKNPWNLACIPGGSSSGSAAA
IAARSATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLGAVAGHDPLDSTSI
DLPVPDYAAALTGQVKGLRLGLPKEYYLEGLDPDVKRALDAAIETYRGLGAEFVEVSLPHTDYAVATYYLIATAEASSNL
ARYDGVRFGHRAAGAANLIDMFRRSRAEGFGAEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFMKAFEQVDALLT
PVAPTPAFKIGEKVDDPLQMYLSDIFTIPVNLAGTCAISVPAGMSAAGLPIGLQLIGRPFGEETILRAAHAFEQATEWHR
HTAQL

Sequences:

>Translated_485_residues
MELFELTLHELHDKLAKREVSSVEATRALLARIEATDSRVNAYITVTPDEALAAAEAADRRIAAGGLTPLTGVPVALKDI
FVTRGIRTTCGSKILGNFIPPYDGTVVAKLREAGAVIVGKLNQDEFAMGSSGESSAFGATKNPWNLACIPGGSSSGSAAA
IAARSATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLGAVAGHDPLDSTSI
DLPVPDYAAALTGQVKGLRLGLPKEYYLEGLDPDVKRALDAAIETYRGLGAEFVEVSLPHTDYAVATYYLIATAEASSNL
ARYDGVRFGHRAAGAANLIDMFRRSRAEGFGAEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFMKAFEQVDALLT
PVAPTPAFKIGEKVDDPLQMYLSDIFTIPVNLAGTCAISVPAGMSAAGLPIGLQLIGRPFGEETILRAAHAFEQATEWHR
HTAQL
>Mature_485_residues
MELFELTLHELHDKLAKREVSSVEATRALLARIEATDSRVNAYITVTPDEALAAAEAADRRIAAGGLTPLTGVPVALKDI
FVTRGIRTTCGSKILGNFIPPYDGTVVAKLREAGAVIVGKLNQDEFAMGSSGESSAFGATKNPWNLACIPGGSSSGSAAA
IAARSATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLGAVAGHDPLDSTSI
DLPVPDYAAALTGQVKGLRLGLPKEYYLEGLDPDVKRALDAAIETYRGLGAEFVEVSLPHTDYAVATYYLIATAEASSNL
ARYDGVRFGHRAAGAANLIDMFRRSRAEGFGAEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFMKAFEQVDALLT
PVAPTPAFKIGEKVDDPLQMYLSDIFTIPVNLAGTCAISVPAGMSAAGLPIGLQLIGRPFGEETILRAAHAFEQATEWHR
HTAQL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=40.4426559356137, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI195972892, Length=500, Percent_Identity=25.2, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=250, Percent_Identity=33.2, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=36.8191721132898, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=24.4979919678715, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=321, Percent_Identity=27.4143302180685, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=515, Percent_Identity=22.9126213592233, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=308, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=308, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=513, Percent_Identity=22.0272904483431, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=459, Percent_Identity=34.2047930283224, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=473, Percent_Identity=29.1754756871036, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=232, Percent_Identity=33.6206896551724, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648113, Length=464, Percent_Identity=41.3793103448276, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=26.1569416498994, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=26.1569416498994, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=26.1569416498994, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=26.1569416498994, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=25.2446183953033, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=27.3809523809524, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648437, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550774, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=23.3739837398374, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=224, Percent_Identity=29.9107142857143, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648439, Length=224, Percent_Identity=29.9107142857143, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_GEOSL (Q746Y7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954421.1
- ProteinModelPortal:   Q746Y7
- SMR:   Q746Y7
- GeneID:   2688205
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3381
- NMPDR:   fig|243231.1.peg.3359
- TIGR:   GSU3381
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   GSUL243231:GSU_3381-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51467; Mature: 51467

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 153-153 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFELTLHELHDKLAKREVSSVEATRALLARIEATDSRVNAYITVTPDEALAAAEAADR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCC
RIAAGGLTPLTGVPVALKDIFVTRGIRTTCGSKILGNFIPPYDGTVVAKLREAGAVIVGK
EEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEE
LNQDEFAMGSSGESSAFGATKNPWNLACIPGGSSSGSAAAIAARSATATLGTDTGGSIRQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCC
PASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLGAVAGHDPLDSTSI
CHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
DLPVPDYAAALTGQVKGLRLGLPKEYYLEGLDPDVKRALDAAIETYRGLGAEFVEVSLPH
CCCCCHHHHHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TDYAVATYYLIATAEASSNLARYDGVRFGHRAAGAANLIDMFRRSRAEGFGAEVKRRIMI
CCHHHEEEEEEEECCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCHHHHHEEEE
GTYALSSGYYDAYYLKAQKVRTLIMQDFMKAFEQVDALLTPVAPTPAFKIGEKVDDPLQM
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHHH
YLSDIFTIPVNLAGTCAISVPAGMSAAGLPIGLQLIGRPFGEETILRAAHAFEQATEWHR
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHH
HTAQL
HHCCC
>Mature Secondary Structure
MELFELTLHELHDKLAKREVSSVEATRALLARIEATDSRVNAYITVTPDEALAAAEAADR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCC
RIAAGGLTPLTGVPVALKDIFVTRGIRTTCGSKILGNFIPPYDGTVVAKLREAGAVIVGK
EEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEE
LNQDEFAMGSSGESSAFGATKNPWNLACIPGGSSSGSAAAIAARSATATLGTDTGGSIRQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCC
PASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLGAVAGHDPLDSTSI
CHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
DLPVPDYAAALTGQVKGLRLGLPKEYYLEGLDPDVKRALDAAIETYRGLGAEFVEVSLPH
CCCCCHHHHHHHCCCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TDYAVATYYLIATAEASSNLARYDGVRFGHRAAGAANLIDMFRRSRAEGFGAEVKRRIMI
CCHHHEEEEEEEECCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCHHHHHEEEE
GTYALSSGYYDAYYLKAQKVRTLIMQDFMKAFEQVDALLTPVAPTPAFKIGEKVDDPLQM
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHHH
YLSDIFTIPVNLAGTCAISVPAGMSAAGLPIGLQLIGRPFGEETILRAAHAFEQATEWHR
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHH
HTAQL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA