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The map label for this gene is pleD [H]

Identifier: 39998465

GI number: 39998465

Start: 3710034

End: 3710960

Strand: Direct

Name: pleD [H]

Synonym: GSU3376

Alternate gene names: 39998465

Gene position: 3710034-3710960 (Clockwise)

Preceding gene: 39998464

Following gene: 39998474

Centisome position: 97.27

GC content: 61.49

Gene sequence:

>927_bases
ATGGCGATGACAGCCCTCGTAATCGACGATTCGGAAGTCCTGCGCGAGGAAATAGTGCGCACCCTCAAGGAGGCCCGGCT
ATTCGAGACGTATCTGGAAGCCCGGGACGGCCTCGAAGGATTCAAGACGCTCCTCAACAACAAGGTGGACCTGGTGCTCT
GCGACCTGGAGATGCCGCGCATGGACGGGTTCCGTTTCCTGTCCATGACCCGGTCCCGGGGTGAGCTCCAGGACCTGCCG
ATCATCCTGCTGACCAGCCGGGAAGACCGGGACACGAAAATTCGCGGACTTGAGCAGGGGGCATCCGATTACGTCACCAA
ACCCTTCGACTCGGGGGAACTGGTTGCCCGGGTCCGGGTGCAGATGAAGGTAAAGGGTCTCCAGGACGAACTGAAGCGCT
CCAACGAGCTGCTGCGCAAATTGTCCATCACCGACCCCCTCACCCACCTGCATAACCGTCGTCACCTGATGGAAATGGTG
GACAAGGAGTTCCAGCGGGCCTCCCGCAAGGGGGGCGCGCTTTCCCTAGTCATTCTCGACATCGACTATTTCAAGAAAAT
CAACGACACCTACGGTCATCAGGAGGGAGACAAGGTGCTGGTGGCTCTGGCAGACATCGTCCGCCTGCGGCTTCGCAGCT
ACGATGTTGCCGCCCGCTACGGGGGCGAAGAGTTCGTCCTGCTGCTGCCGGAAACCCCTCTGCAGGAGGCCCAGGCCATT
GCCGAGCGGCTGCGGCTGGAGGTGCAGGATCACGTCTTCGACGGGAGCCTCCACGGACAGGTCATCACCATCAGCCTGGG
CGTGGCTACCTATCCATCACCCCGCATCGAGGGGACCGACTCCCTCTTCAGGCAGGCCGACGAGGCCCTCTACCGGGCCA
AGCAGAACGGTCGCAACAGGGTGGAGCTCATGTCAGGCTCGACATAA

Upstream 100 bases:

>100_bases
CCGCCATACTCTCGCAGTTCCTCAACACGATATTCCCTCGGCCGTGACCGGGGCGGGACGTTCCCACGGATTACCCATCC
GCGCGGAGAAGCATGAAACC

Downstream 100 bases:

>100_bases
CAGATGGAGTCCGCACTCGCCGCAACGGGGGCGGGTGGTGCAGTGCCGCTTGCAATGCTCCACGATGAGGGCGTGATATT
CGTTGAACAGCGTAGTGTCT

Product: GGDEF/response regulator receiver domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAMTALVIDDSEVLREEIVRTLKEARLFETYLEARDGLEGFKTLLNNKVDLVLCDLEMPRMDGFRFLSMTRSRGELQDLP
IILLTSREDRDTKIRGLEQGASDYVTKPFDSGELVARVRVQMKVKGLQDELKRSNELLRKLSITDPLTHLHNRRHLMEMV
DKEFQRASRKGGALSLVILDIDYFKKINDTYGHQEGDKVLVALADIVRLRLRSYDVAARYGGEEFVLLLPETPLQEAQAI
AERLRLEVQDHVFDGSLHGQVITISLGVATYPSPRIEGTDSLFRQADEALYRAKQNGRNRVELMSGST

Sequences:

>Translated_308_residues
MAMTALVIDDSEVLREEIVRTLKEARLFETYLEARDGLEGFKTLLNNKVDLVLCDLEMPRMDGFRFLSMTRSRGELQDLP
IILLTSREDRDTKIRGLEQGASDYVTKPFDSGELVARVRVQMKVKGLQDELKRSNELLRKLSITDPLTHLHNRRHLMEMV
DKEFQRASRKGGALSLVILDIDYFKKINDTYGHQEGDKVLVALADIVRLRLRSYDVAARYGGEEFVLLLPETPLQEAQAI
AERLRLEVQDHVFDGSLHGQVITISLGVATYPSPRIEGTDSLFRQADEALYRAKQNGRNRVELMSGST
>Mature_307_residues
AMTALVIDDSEVLREEIVRTLKEARLFETYLEARDGLEGFKTLLNNKVDLVLCDLEMPRMDGFRFLSMTRSRGELQDLPI
ILLTSREDRDTKIRGLEQGASDYVTKPFDSGELVARVRVQMKVKGLQDELKRSNELLRKLSITDPLTHLHNRRHLMEMVD
KEFQRASRKGGALSLVILDIDYFKKINDTYGHQEGDKVLVALADIVRLRLRSYDVAARYGGEEFVLLLPETPLQEAQAIA
ERLRLEVQDHVFDGSLHGQVITISLGVATYPSPRIEGTDSLFRQADEALYRAKQNGRNRVELMSGST

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=42.4418604651163, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=40.4907975460123, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=38.4615384615385, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=38.9937106918239, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=39.2857142857143, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=33.5227272727273, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=36.8983957219251, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=39.1566265060241, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=34.2245989304813, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=35.4285714285714, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=32.0754716981132, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87082012, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=35.7723577235772, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=37.1681415929204, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788191, Length=109, Percent_Identity=34.8623853211009, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=175, Percent_Identity=27.4285714285714, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=121, Percent_Identity=33.0578512396694, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787056, Length=165, Percent_Identity=32.1212121212121, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788956, Length=176, Percent_Identity=32.9545454545455, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=34.9593495934959, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=133, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787229, Length=81, Percent_Identity=45.679012345679, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=32.7731092436975, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787375, Length=121, Percent_Identity=33.8842975206612, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=29.2307692307692, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35077; Mature: 34946

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTALVIDDSEVLREEIVRTLKEARLFETYLEARDGLEGFKTLLNNKVDLVLCDLEMPR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCC
MDGFRFLSMTRSRGELQDLPIILLTSREDRDTKIRGLEQGASDYVTKPFDSGELVARVRV
CCCHHHHHHHHCCCCHHCCCEEEEECCCCCCHHHHHHHCCCHHCCCCCCCCCHHHHHHHH
QMKVKGLQDELKRSNELLRKLSITDPLTHLHNRRHLMEMVDKEFQRASRKGGALSLVILD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IDYFKKINDTYGHQEGDKVLVALADIVRLRLRSYDVAARYGGEEFVLLLPETPLQEAQAI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AERLRLEVQDHVFDGSLHGQVITISLGVATYPSPRIEGTDSLFRQADEALYRAKQNGRNR
HHHHHHHHHHHEECCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
VELMSGST
EEEECCCC
>Mature Secondary Structure 
AMTALVIDDSEVLREEIVRTLKEARLFETYLEARDGLEGFKTLLNNKVDLVLCDLEMPR
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCC
MDGFRFLSMTRSRGELQDLPIILLTSREDRDTKIRGLEQGASDYVTKPFDSGELVARVRV
CCCHHHHHHHHCCCCHHCCCEEEEECCCCCCHHHHHHHCCCHHCCCCCCCCCHHHHHHHH
QMKVKGLQDELKRSNELLRKLSITDPLTHLHNRRHLMEMVDKEFQRASRKGGALSLVILD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IDYFKKINDTYGHQEGDKVLVALADIVRLRLRSYDVAARYGGEEFVLLLPETPLQEAQAI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AERLRLEVQDHVFDGSLHGQVITISLGVATYPSPRIEGTDSLFRQADEALYRAKQNGRNR
HHHHHHHHHHHEECCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCE
VELMSGST
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA