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The map label for this gene is pckA

Identifier: 39998474

GI number: 39998474

Start: 3721688

End: 3723544

Strand: Direct

Name: pckA

Synonym: GSU3385

Alternate gene names: 39998474

Gene position: 3721688-3723544 (Clockwise)

Preceding gene: 39998465

Following gene: 39998477

Centisome position: 97.58

GC content: 61.39

Gene sequence:

>1857_bases
ATGAGTTATGAACTTCCGAACAACGAAAAGCTTCTGAAGTGGGTCGAAGAGGTGGCGATCCTCTGCGAGCCGGACCGCAT
CCACTGGTGCGACGGTTCCCAGGAGGAGTACGACGAACTCTGCGAACTGCTGGTGCAAAACGGCACTTTCAGGAAGCTTA
ATCCGGAAAAGCGTCCCAATAGCTACCTGGCGTGGTCTGATCCCATCGACGTGGCGCGGGTCGAGGACCGTACGTTTATC
TGCAGCCTCTCCAAGCAGGATGCGGGCCCCACGAACAACTGGATGAACCCCAAGGAGATGAAGGAGAAGCTTCTGGGCCT
CTTTAAAGGGTGCATGAAGGGACGGACCATGTATGTCATCCCCTTCAGCATGGGTCCCCTCGGCTCCCCCATTGCCAAGA
TCGGCGTTGAAATAACCGACTCCCCCTACGTCGTAGTCAACATGCGAATCATGACCAGAATGGGGAAAAAGGTCCTTGAT
ATCCTCGGCGAACAAGGCGAATTCGTGCCGTGCCTCCACTCGGTGGGAGCCCCCCTGGAGCAGGGCCAGAAGGACAGCAC
CTGGCCCTGCAACAAGGAAAAGTACATCGTTCACTTCCCGGAGGAGCGCTCCATCTGGTCCTTCGGTAGCGGCTACGGCG
GCAACGCCCTTCTCGGCAAAAAGTGCCTGGCTCTCCGGATTGCCTCCAAGCAGGGCAAGGACGAGGGATGGCTGGCCGAG
CACATGCTGATTCTCGGCGTCGAGTCCCCCACCGGTGATAAAACTTACGTCGGCGCGGCCTTCCCCAGCGCCTGCGGCAA
AACCAACTTCGCCATGCTGATCCCGCCCAAACCGCTGCAGGATAAGGGGTGGAAAGTAACCACCATCGGCGACGACATCG
CCTGGATCAAGCCGGGACCCGACGGCCAGGTGTACGCCATCAACCCCGAATACGGCTACTTCGGGGTCGCCCCCGGCACC
AACGTCAAGACCAACCCCAATGCCATGGCCTCTTGCTCCGCCAACACCATCTTCACCAACGTGGCCCTTACCCCCGATGA
CGATGTCTGGTGGGAGGGGATGACTGACGAGCCCCCCGCCCAACTCACCGACTGGCAGGGGAACCCCTGGACCCCCGACT
GCGGTCGTCCGGCAGCCCATCCCAATGCCCGCTTCACCTCGCCGGCCAGCCAGTGCCCCTCCATCGACCCGGACTGGGAG
AACCCCAAAGGGGTGCCCATGAGCGCCTTCATCTTCGGCGGGCGCCGCAAAGACACCGTGCCGCTGGTCTATCAGGCATT
CAACTGGAGCAATGGCGTCTACCTGGCCGCGACGCTCGGCTCTGAAACCACCGCCGCTGCCGTGGGCGCCGTGGGCAACG
TCCGCCGCGATCCTTTTGCCATGCTGCCGTTCTGCGGCTACCATATGGCCGATTACTTCGGCCACTGGCTGCAATTCGGC
CGTTCCATCCCGCGTCCGCCGCGGATCTTCAGCGTCAACTGGTTCCGCAAGGACGCCAACGGCAAGTTCATCTGGCCCGG
CTACGGGGACAACATGAGGGTCCTCAAGTGGATCGTGGAGCGGGTACAGGGCAAAGCAGGCGCCGTTGAATCACCTCTGG
GCTGGATGCCCCGCTACGAGGATATGGACTGGGACGGTCTGGACATGCCGCGCGAAAAGTTCAACGAGCTGACCTCGGTG
GACCGCGACGTCTGGCAGAACGAGCTCATCTCCCACGAAGAGCTGTTCGTCAAGCTCTTTGACCGGCTCCCCAAGGAGTT
CCTCCACATGCGCGAACTGATCCTCTCCAGCCTCTGGCGCTCGCCCGAGCACTGGGAGCAGATCGGCGACGTTCATCCGG
AAGGGTGGAACGAGTAG

Upstream 100 bases:

>100_bases
AGGGCAGGTGATAACGCCGTTCTCGTCGGTTCACTCACGAATAACGAACAAAGGTGTTCGTGGGACTTAAGTACACATTT
AGACAGGGGAGGGAAGAGGA

Downstream 100 bases:

>100_bases
CGGCACCACTCCGCTTTCGGGCACACGAAAAAGGAGGAGCCGGGATCATCCGGCTCCTCCTTTTTTCGTCTTTCAGCCTC
ACAGGCGACCAGCAACGACG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MSYELPNNEKLLKWVEEVAILCEPDRIHWCDGSQEEYDELCELLVQNGTFRKLNPEKRPNSYLAWSDPIDVARVEDRTFI
CSLSKQDAGPTNNWMNPKEMKEKLLGLFKGCMKGRTMYVIPFSMGPLGSPIAKIGVEITDSPYVVVNMRIMTRMGKKVLD
ILGEQGEFVPCLHSVGAPLEQGQKDSTWPCNKEKYIVHFPEERSIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAE
HMLILGVESPTGDKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTIGDDIAWIKPGPDGQVYAINPEYGYFGVAPGT
NVKTNPNAMASCSANTIFTNVALTPDDDVWWEGMTDEPPAQLTDWQGNPWTPDCGRPAAHPNARFTSPASQCPSIDPDWE
NPKGVPMSAFIFGGRRKDTVPLVYQAFNWSNGVYLAATLGSETTAAAVGAVGNVRRDPFAMLPFCGYHMADYFGHWLQFG
RSIPRPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAGAVESPLGWMPRYEDMDWDGLDMPREKFNELTSV
DRDVWQNELISHEELFVKLFDRLPKEFLHMRELILSSLWRSPEHWEQIGDVHPEGWNE

Sequences:

>Translated_618_residues
MSYELPNNEKLLKWVEEVAILCEPDRIHWCDGSQEEYDELCELLVQNGTFRKLNPEKRPNSYLAWSDPIDVARVEDRTFI
CSLSKQDAGPTNNWMNPKEMKEKLLGLFKGCMKGRTMYVIPFSMGPLGSPIAKIGVEITDSPYVVVNMRIMTRMGKKVLD
ILGEQGEFVPCLHSVGAPLEQGQKDSTWPCNKEKYIVHFPEERSIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAE
HMLILGVESPTGDKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTIGDDIAWIKPGPDGQVYAINPEYGYFGVAPGT
NVKTNPNAMASCSANTIFTNVALTPDDDVWWEGMTDEPPAQLTDWQGNPWTPDCGRPAAHPNARFTSPASQCPSIDPDWE
NPKGVPMSAFIFGGRRKDTVPLVYQAFNWSNGVYLAATLGSETTAAAVGAVGNVRRDPFAMLPFCGYHMADYFGHWLQFG
RSIPRPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAGAVESPLGWMPRYEDMDWDGLDMPREKFNELTSV
DRDVWQNELISHEELFVKLFDRLPKEFLHMRELILSSLWRSPEHWEQIGDVHPEGWNE
>Mature_617_residues
SYELPNNEKLLKWVEEVAILCEPDRIHWCDGSQEEYDELCELLVQNGTFRKLNPEKRPNSYLAWSDPIDVARVEDRTFIC
SLSKQDAGPTNNWMNPKEMKEKLLGLFKGCMKGRTMYVIPFSMGPLGSPIAKIGVEITDSPYVVVNMRIMTRMGKKVLDI
LGEQGEFVPCLHSVGAPLEQGQKDSTWPCNKEKYIVHFPEERSIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAEH
MLILGVESPTGDKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTIGDDIAWIKPGPDGQVYAINPEYGYFGVAPGTN
VKTNPNAMASCSANTIFTNVALTPDDDVWWEGMTDEPPAQLTDWQGNPWTPDCGRPAAHPNARFTSPASQCPSIDPDWEN
PKGVPMSAFIFGGRRKDTVPLVYQAFNWSNGVYLAATLGSETTAAAVGAVGNVRRDPFAMLPFCGYHMADYFGHWLQFGR
SIPRPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAGAVESPLGWMPRYEDMDWDGLDMPREKFNELTSVD
RDVWQNELISHEELFVKLFDRLPKEFLHMRELILSSLWRSPEHWEQIGDVHPEGWNE

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=596, Percent_Identity=52.3489932885906, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI187281517, Length=575, Percent_Identity=52.1739130434783, Blast_Score=594, Evalue=1e-170,
Organism=Homo sapiens, GI66346723, Length=376, Percent_Identity=56.3829787234042, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17555492, Length=585, Percent_Identity=51.2820512820513, Blast_Score=581, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71989645, Length=576, Percent_Identity=50.5208333333333, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989658, Length=576, Percent_Identity=50.5208333333333, Blast_Score=560, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564411, Length=538, Percent_Identity=53.1598513011152, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989653, Length=562, Percent_Identity=51.067615658363, Blast_Score=552, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32564407, Length=493, Percent_Identity=54.158215010142, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71989649, Length=486, Percent_Identity=52.2633744855967, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI32564409, Length=339, Percent_Identity=50.7374631268437, Blast_Score=345, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI17540956, Length=515, Percent_Identity=26.6019417475728, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24655085, Length=568, Percent_Identity=52.6408450704225, Blast_Score=593, Evalue=1e-169,
Organism=Drosophila melanogaster, GI24655082, Length=577, Percent_Identity=51.2998266897747, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI161077226, Length=546, Percent_Identity=52.014652014652, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24655088, Length=465, Percent_Identity=55.6989247311828, Blast_Score=523, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_GEOSL (Q746Y3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954425.1
- ProteinModelPortal:   Q746Y3
- GeneID:   2688197
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3385
- NMPDR:   fig|243231.1.peg.3363
- TIGR:   GSU3385
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   GSUL243231:GSU_3385-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 69614; Mature: 69483

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 265-265 BINDING 73-73 BINDING 214-214 BINDING 221-221 BINDING 263-263 BINDING 384-384 BINDING 415-415

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYELPNNEKLLKWVEEVAILCEPDRIHWCDGSQEEYDELCELLVQNGTFRKLNPEKRPN
CCCCCCCCHHHHHHHHHHHHEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
SYLAWSDPIDVARVEDRTFICSLSKQDAGPTNNWMNPKEMKEKLLGLFKGCMKGRTMYVI
CEEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
PFSMGPLGSPIAKIGVEITDSPYVVVNMRIMTRMGKKVLDILGEQGEFVPCLHSVGAPLE
EECCCCCCCCHHHHCCEECCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCHH
QGQKDSTWPCNKEKYIVHFPEERSIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAE
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCHHEE
HMLILGVESPTGDKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTIGDDIAWIKPGP
EEEEEEECCCCCCCEEEECCCCHHCCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCCC
DGQVYAINPEYGYFGVAPGTNVKTNPNAMASCSANTIFTNVALTPDDDVWWEGMTDEPPA
CCCEEEECCCCCEEECCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCC
QLTDWQGNPWTPDCGRPAAHPNARFTSPASQCPSIDPDWENPKGVPMSAFIFGGRRKDTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
PLVYQAFNWSNGVYLAATLGSETTAAAVGAVGNVRRDPFAMLPFCGYHMADYFGHWLQFG
EEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHC
RSIPRPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAGAVESPLGWMPRYE
CCCCCCCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DMDWDGLDMPREKFNELTSVDRDVWQNELISHEELFVKLFDRLPKEFLHMRELILSSLWR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPEHWEQIGDVHPEGWNE
CHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SYELPNNEKLLKWVEEVAILCEPDRIHWCDGSQEEYDELCELLVQNGTFRKLNPEKRPN
CCCCCCCHHHHHHHHHHHHEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
SYLAWSDPIDVARVEDRTFICSLSKQDAGPTNNWMNPKEMKEKLLGLFKGCMKGRTMYVI
CEEEECCCCCEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
PFSMGPLGSPIAKIGVEITDSPYVVVNMRIMTRMGKKVLDILGEQGEFVPCLHSVGAPLE
EECCCCCCCCHHHHCCEECCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCHH
QGQKDSTWPCNKEKYIVHFPEERSIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAE
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCHHEE
HMLILGVESPTGDKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTIGDDIAWIKPGP
EEEEEEECCCCCCCEEEECCCCHHCCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCCC
DGQVYAINPEYGYFGVAPGTNVKTNPNAMASCSANTIFTNVALTPDDDVWWEGMTDEPPA
CCCEEEECCCCCEEECCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCC
QLTDWQGNPWTPDCGRPAAHPNARFTSPASQCPSIDPDWENPKGVPMSAFIFGGRRKDTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
PLVYQAFNWSNGVYLAATLGSETTAAAVGAVGNVRRDPFAMLPFCGYHMADYFGHWLQFG
EEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHC
RSIPRPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAGAVESPLGWMPRYE
CCCCCCCEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DMDWDGLDMPREKFNELTSVDRDVWQNELISHEELFVKLFDRLPKEFLHMRELILSSLWR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPEHWEQIGDVHPEGWNE
CHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA