| Definition | Geobacter sulfurreducens PCA chromosome, complete genome. |
|---|---|
| Accession | NC_002939 |
| Length | 3,814,139 |
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The map label for this gene is anfA [H]
Identifier: 39998452
GI number: 39998452
Start: 3694820
End: 3697111
Strand: Direct
Name: anfA [H]
Synonym: GSU3363
Alternate gene names: 39998452
Gene position: 3694820-3697111 (Clockwise)
Preceding gene: 39998449
Following gene: 39998453
Centisome position: 96.87
GC content: 58.73
Gene sequence:
>2292_bases ATGGAGCCGATAATTACCGACAAGGAAAAGTGCCGCAAGTGCTACTGCTGCGTCAGGAGCTGCCCGGTCAAGGCAATCAA GGTTGAAAAACGCTACACCGAAATTATCTTTGACCGCTGCATCGGCTGCGGCAACTGTCTGAGCAATTGCCCCCAACGGG CAAAGATGGTGGCCGACAAAGTGGGTGTTACCGAGAGTCTTCTCACCTCGGAAGACAAGGTAATCGCCGTGCTCGGATCA TCATTTCCAGCCTTTTTTCACAATGTTGCACCGGGCCAGCTTGTGGCCGGCCTCAAGCGGATCGGCTTCCGTGAAGTCCA CGAAGGGGCCTACGGTGCTGAACTGATCGCTGACGATTACGCCCGTATTACCGGCGATAATGACCGGACCTACATTTCAT CCCACTGCCCGGCCATCGTTGACCTGATCGAGCGACACTACCCGAAACTGCTCAGAAACCTGGTGCCCGTCGTTTCTCCC ATGATCGCCATGGGCCGCTATCTCAAGGAAGTTCTGGGCCCCAAGACCAAGGTGGTCTACATCAGCTCCTGCATTGCCGC AAAGTTCGAAACCCAGATGACCGAAACGCGGGGAGCCATCGACATTGTTCTCACCTATCGGGAACTGGAGGGCATCTTCC GCAGCCGGCAGATTTCCCTCCCGGCCCTCGATGCGCTTCCCTTCGACGGGCTTGCGCCCACGGTGGGACGACTTTTCCCC ATCAACGAGGGAACCTTCCGGTCGTTCTCCATGTCGGCGGACCCCCTTGACACTGAAATAGTCTCCGCCTGCGGCGAAGT AAACGTCATGGGCATCATCAGAGACTTGGCTGCAGGCAGGATCGCCCCCCGCTTTGCCGATCTGCGCTTCTGCTATGACG GGTGCATCGGCGGTCCCGGCCGCAATAGCGAACTGACCGAATTCTACCGCCGCAACCTGATCATTAACCATTACAAGCAG GACATCCCCTACGAGACGGCTCCGCACTATCTCTCCTCCCGTGAGCAGACCGGCCTGGACCGCTCTTTTGCCAGCAAGCA CGCGCGCCTCGAGTCGCCAAAAACAAATGATATCAAGAAAATACTGCAAGCCACCAGCAAGTACTCGGTCAAGGACGAAC TCAATTGCCGCGCCTGCGGCTATCGCACCTGCCGCGAGTATGCCGTGGCGGTCTACCAGGGCTTGGCCGAGATCGAGATG TGCCTTCCCTACAACCTCCAGCAACTGGAAGAGGATCGTGGTCGCCTGATCCAGAAGTACGAGCTCGCCCGTCGAGAACT CGACCGCGAGTATACTGACGAGTTCATCGTCGGCAACGACCGCAAGACCTTGGAAGTACTTGATCTCATCAAGCAAGTGG GGCCGACGCCGACGACGGTTCTGATCCGGGGCGAGTCGGGAACCGGCAAGGAACTCACCGCCCGAGCCATTCATCGCTTC AGCAAGCGCAACGATAAGCCCCTGGTGACCGTGAACTGCACCACTATCACCGACTCCCTTCTGGAGAGCGAACTCTTCGG ACACAAGCGGGGGGCCTTCACCGGTGCCATCGCCGAGAAAAAGGGGCTGTTCGAGGCCGCCGACGGCGGCACCATTTTCC TGGATGAAATCGGCGATATCACGCCGAAATTGCAGGCAGAGCTCCTGCGTGTCCTCGACATGGGCGAGGTGCGTCCCGTT GGGGGTACGACCGCCCGCAAGGTCGACGTACGCCTCATTGCCGCAACCAACCGGAACCTCGAAGAAGGAGTACGGGAGGG CTGGTTCCGCGAGGATCTGTATTATCGCCTCAACGTTTTCACCATTACCATGCCGCCTCTGCGCAACCGGGTGGAGTCGA TTCCGATCCTGGCGCACCACTTCATGGAAAAAGCCAGCACCAAACTGAACAAACGGCTCTCAGCCATCGAAGAGCGGGCC GTCATCGCCCTCACCAAGTACCCTTGGCCCGGCAATATCCGCGAGATGCAGAACGTCATCGAACGGGCTGCGGTTCTTGC CCACGACGATGTTATCCACCTGGAGAACCTCCCCCTGGCCCTTTCGGAAAATCTGGCAGGAAGCCCGACCGCCGACCTGG ATATCCGGGCTTCCTTCCGCGCGGAGCGCGAAAGGCATGTGGTCAAGCTCGAGAAGAAGCTGATCCAGCGCTATCTGGCC GAGGCAAACGGCAATGTGAGCCGTGCCGCGCGGCTCGCCAACATTCCCCGCCGAACCTTTTACCGCCTGCTCGACAAGTA TCGCCTCAAAGACAAAGAGGTGCGGGATATGCCGCAGGGGGACAGACAATAG
Upstream 100 bases:
>100_bases CCAACCCACTGCGCCACATCTGGCACAGCGTATACTTTATGTGTCACTTATTGCACACCACCCCCTCAATAGTGTATGGT TCACATGTACAGGAGGCGAC
Downstream 100 bases:
>100_bases TGTGCACTTTTGGCCAAGCCTGTGCCAACCGTGACACAAGGATGTATACGAAGATTTTTATTAGATAATTTTTATATTTA ACTTAATAATGATTTCGACA
Product: sigma-54 dependent transcriptional regulator, Fis family
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 763; Mature: 763
Protein sequence:
>763_residues MEPIITDKEKCRKCYCCVRSCPVKAIKVEKRYTEIIFDRCIGCGNCLSNCPQRAKMVADKVGVTESLLTSEDKVIAVLGS SFPAFFHNVAPGQLVAGLKRIGFREVHEGAYGAELIADDYARITGDNDRTYISSHCPAIVDLIERHYPKLLRNLVPVVSP MIAMGRYLKEVLGPKTKVVYISSCIAAKFETQMTETRGAIDIVLTYRELEGIFRSRQISLPALDALPFDGLAPTVGRLFP INEGTFRSFSMSADPLDTEIVSACGEVNVMGIIRDLAAGRIAPRFADLRFCYDGCIGGPGRNSELTEFYRRNLIINHYKQ DIPYETAPHYLSSREQTGLDRSFASKHARLESPKTNDIKKILQATSKYSVKDELNCRACGYRTCREYAVAVYQGLAEIEM CLPYNLQQLEEDRGRLIQKYELARRELDREYTDEFIVGNDRKTLEVLDLIKQVGPTPTTVLIRGESGTGKELTARAIHRF SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAIAEKKGLFEAADGGTIFLDEIGDITPKLQAELLRVLDMGEVRPV GGTTARKVDVRLIAATNRNLEEGVREGWFREDLYYRLNVFTITMPPLRNRVESIPILAHHFMEKASTKLNKRLSAIEERA VIALTKYPWPGNIREMQNVIERAAVLAHDDVIHLENLPLALSENLAGSPTADLDIRASFRAERERHVVKLEKKLIQRYLA EANGNVSRAARLANIPRRTFYRLLDKYRLKDKEVRDMPQGDRQ
Sequences:
>Translated_763_residues MEPIITDKEKCRKCYCCVRSCPVKAIKVEKRYTEIIFDRCIGCGNCLSNCPQRAKMVADKVGVTESLLTSEDKVIAVLGS SFPAFFHNVAPGQLVAGLKRIGFREVHEGAYGAELIADDYARITGDNDRTYISSHCPAIVDLIERHYPKLLRNLVPVVSP MIAMGRYLKEVLGPKTKVVYISSCIAAKFETQMTETRGAIDIVLTYRELEGIFRSRQISLPALDALPFDGLAPTVGRLFP INEGTFRSFSMSADPLDTEIVSACGEVNVMGIIRDLAAGRIAPRFADLRFCYDGCIGGPGRNSELTEFYRRNLIINHYKQ DIPYETAPHYLSSREQTGLDRSFASKHARLESPKTNDIKKILQATSKYSVKDELNCRACGYRTCREYAVAVYQGLAEIEM CLPYNLQQLEEDRGRLIQKYELARRELDREYTDEFIVGNDRKTLEVLDLIKQVGPTPTTVLIRGESGTGKELTARAIHRF SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAIAEKKGLFEAADGGTIFLDEIGDITPKLQAELLRVLDMGEVRPV GGTTARKVDVRLIAATNRNLEEGVREGWFREDLYYRLNVFTITMPPLRNRVESIPILAHHFMEKASTKLNKRLSAIEERA VIALTKYPWPGNIREMQNVIERAAVLAHDDVIHLENLPLALSENLAGSPTADLDIRASFRAERERHVVKLEKKLIQRYLA EANGNVSRAARLANIPRRTFYRLLDKYRLKDKEVRDMPQGDRQ >Mature_763_residues MEPIITDKEKCRKCYCCVRSCPVKAIKVEKRYTEIIFDRCIGCGNCLSNCPQRAKMVADKVGVTESLLTSEDKVIAVLGS SFPAFFHNVAPGQLVAGLKRIGFREVHEGAYGAELIADDYARITGDNDRTYISSHCPAIVDLIERHYPKLLRNLVPVVSP MIAMGRYLKEVLGPKTKVVYISSCIAAKFETQMTETRGAIDIVLTYRELEGIFRSRQISLPALDALPFDGLAPTVGRLFP INEGTFRSFSMSADPLDTEIVSACGEVNVMGIIRDLAAGRIAPRFADLRFCYDGCIGGPGRNSELTEFYRRNLIINHYKQ DIPYETAPHYLSSREQTGLDRSFASKHARLESPKTNDIKKILQATSKYSVKDELNCRACGYRTCREYAVAVYQGLAEIEM CLPYNLQQLEEDRGRLIQKYELARRELDREYTDEFIVGNDRKTLEVLDLIKQVGPTPTTVLIRGESGTGKELTARAIHRF SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAIAEKKGLFEAADGGTIFLDEIGDITPKLQAELLRVLDMGEVRPV GGTTARKVDVRLIAATNRNLEEGVREGWFREDLYYRLNVFTITMPPLRNRVESIPILAHHFMEKASTKLNKRLSAIEERA VIALTKYPWPGNIREMQNVIERAAVLAHDDVIHLENLPLALSENLAGSPTADLDIRASFRAERERHVVKLEKKLIQRYLA EANGNVSRAARLANIPRRTFYRLLDKYRLKDKEVRDMPQGDRQ
Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]
COG id: COG3604
COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 sigma-54 factor interaction domain [H]
Homologues:
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=46.6237942122186, Blast_Score=263, Evalue=3e-71, Organism=Escherichia coli, GI1788550, Length=360, Percent_Identity=40.2777777777778, Blast_Score=261, Evalue=1e-70, Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=54.1125541125541, Blast_Score=252, Evalue=5e-68, Organism=Escherichia coli, GI1788905, Length=323, Percent_Identity=41.7956656346749, Blast_Score=247, Evalue=2e-66, Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=42.2360248447205, Blast_Score=241, Evalue=1e-64, Organism=Escherichia coli, GI1790299, Length=378, Percent_Identity=39.4179894179894, Blast_Score=240, Evalue=3e-64, Organism=Escherichia coli, GI1789087, Length=323, Percent_Identity=43.343653250774, Blast_Score=237, Evalue=2e-63, Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=41.2140575079872, Blast_Score=219, Evalue=4e-58, Organism=Escherichia coli, GI87081872, Length=334, Percent_Identity=40.1197604790419, Blast_Score=209, Evalue=4e-55, Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=39.3586005830904, Blast_Score=202, Evalue=8e-53, Organism=Escherichia coli, GI1787583, Length=228, Percent_Identity=42.5438596491228, Blast_Score=181, Evalue=2e-46, Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=39.4736842105263, Blast_Score=142, Evalue=1e-34, Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=31.0679611650485, Blast_Score=134, Evalue=3e-32,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR003018 - InterPro: IPR020441 - InterPro: IPR009057 - InterPro: IPR002197 - InterPro: IPR002078 [H]
Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]
EC number: NA
Molecular weight: Translated: 85962; Mature: 85962
Theoretical pI: Translated: 8.45; Mature: 8.45
Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4 ; PS00198 4FE4S_FERREDOXIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.5 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 2.5 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEPIITDKEKCRKCYCCVRSCPVKAIKVEKRYTEIIFDRCIGCGNCLSNCPQRAKMVADK CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHH VGVTESLLTSEDKVIAVLGSSFPAFFHNVAPGQLVAGLKRIGFREVHEGAYGAELIADDY HCCHHHHHCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHCHHHHHCCCCCHHHHHCCH ARITGDNDRTYISSHCPAIVDLIERHYPKLLRNLVPVVSPMIAMGRYLKEVLGPKTKVVY HHCCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE ISSCIAAKFETQMTETRGAIDIVLTYRELEGIFRSRQISLPALDALPFDGLAPTVGRLFP EHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCEEE INEGTFRSFSMSADPLDTEIVSACGEVNVMGIIRDLAAGRIAPRFADLRFCYDGCIGGPG CCCCCCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC RNSELTEFYRRNLIINHYKQDIPYETAPHYLSSREQTGLDRSFASKHARLESPKTNDIKK CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHCCCCHHHHHHHHHCCCCCHHHHHH ILQATSKYSVKDELNCRACGYRTCREYAVAVYQGLAEIEMCLPYNLQQLEEDRGRLIQKY HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH ELARRELDREYTDEFIVGNDRKTLEVLDLIKQVGPTPTTVLIRGESGTGKELTARAIHRF HHHHHHHCHHHCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAIAEKKGLFEAADGGTIFLDEIGDI HCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCEEEECCCCCC TPKLQAELLRVLDMGEVRPVGGTTARKVDVRLIAATNRNLEEGVREGWFREDLYYRLNVF CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHCCEEEEEEE TITMPPLRNRVESIPILAHHFMEKASTKLNKRLSAIEERAVIALTKYPWPGNIREMQNVI EEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHH ERAAVLAHDDVIHLENLPLALSENLAGSPTADLDIRASFRAERERHVVKLEKKLIQRYLA HHHHHHHCCCEEEECCCCCHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH EANGNVSRAARLANIPRRTFYRLLDKYRLKDKEVRDMPQGDRQ HCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCCC >Mature Secondary Structure MEPIITDKEKCRKCYCCVRSCPVKAIKVEKRYTEIIFDRCIGCGNCLSNCPQRAKMVADK CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHH VGVTESLLTSEDKVIAVLGSSFPAFFHNVAPGQLVAGLKRIGFREVHEGAYGAELIADDY HCCHHHHHCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHCHHHHHCCCCCHHHHHCCH ARITGDNDRTYISSHCPAIVDLIERHYPKLLRNLVPVVSPMIAMGRYLKEVLGPKTKVVY HHCCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE ISSCIAAKFETQMTETRGAIDIVLTYRELEGIFRSRQISLPALDALPFDGLAPTVGRLFP EHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCEEE INEGTFRSFSMSADPLDTEIVSACGEVNVMGIIRDLAAGRIAPRFADLRFCYDGCIGGPG CCCCCCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC RNSELTEFYRRNLIINHYKQDIPYETAPHYLSSREQTGLDRSFASKHARLESPKTNDIKK CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHCCCCHHHHHHHHHCCCCCHHHHHH ILQATSKYSVKDELNCRACGYRTCREYAVAVYQGLAEIEMCLPYNLQQLEEDRGRLIQKY HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH ELARRELDREYTDEFIVGNDRKTLEVLDLIKQVGPTPTTVLIRGESGTGKELTARAIHRF HHHHHHHCHHHCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAIAEKKGLFEAADGGTIFLDEIGDI HCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCEEEECCCCCC TPKLQAELLRVLDMGEVRPVGGTTARKVDVRLIAATNRNLEEGVREGWFREDLYYRLNVF CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHCCEEEEEEE TITMPPLRNRVESIPILAHHFMEKASTKLNKRLSAIEERAVIALTKYPWPGNIREMQNVI EEECCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHH ERAAVLAHDDVIHLENLPLALSENLAGSPTADLDIRASFRAERERHVVKLEKKLIQRYLA HHHHHHHCCCEEEECCCCCHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH EANGNVSRAARLANIPRRTFYRLLDKYRLKDKEVRDMPQGDRQ HCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2722750 [H]