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The map label for this gene is purA

Identifier: 39998397

GI number: 39998397

Start: 3632453

End: 3633754

Strand: Direct

Name: purA

Synonym: GSU3308

Alternate gene names: 39998397

Gene position: 3632453-3633754 (Clockwise)

Preceding gene: 39998396

Following gene: 39998400

Centisome position: 95.24

GC content: 60.83

Gene sequence:

>1302_bases
ATGGCTAACGTGGTTGTGGTTGGTGCCCAGTGGGGCGACGAAGGCAAGGGCAAGGTCGTTGACATCTATACCGAGCATGC
GGACGACGTGGTCCGTTACCAGGGGGGAAACAACGCCGGGCACACCCTGGTCGTTGGGGAAGAAAAGGTGGTCCTGCACC
TGATCCCCTCGGGCATTCTCCACGAGGGTAAACGGTGCATCATCGGCAACGGCGTGGTTCTCGACCCCGAGGTCTTCATC
CAGGAGATCACCCGCCTCAAGGACAAGGGCCGCCTCAAGGACGACCGCGCGCTCCTGGTCAGCGAATCGATCCACATCAT
CATGCCCTACCACAAACGGATCGACATCGCCCGCGAAGCCAAGAGCGGCGAAAAGAAAATCGGTACCACCGGCCGGGGGA
TCGGCCCCACCTATGAGGACAAAATCGGCCGGCGCGGTATCCGGCTCATGGACCTCCTCGATCGCGACGTGTTTGCGCGC
AAGTTGAAGGAAAACCTCGAAGAGAAGAACGTCATCCTCGAAAAACTCCTGGGCGACAAGCCCTTTACCTTTGAGGAGAT
TTACGAGCAGTACTGCGGCTACGCCGACATCCTCAGAAACTACGTGGCCGACACAAGCCTCATCCTTTACAACGACAGCA
AGGCCGGCAAGAAACTCCTCTTCGAGGGAGCCCAGGGGACACTCCTCGACGTGGACCACGGCACTTACCCCTTTGTCACA
TCCTCATCCACCTGTGCAGGCGGTGCCTGTACCGGCACCGGCGTGAGCCCCCGGGACATCCACGAGATCATCGGCATATC
CAAGGCCTATGTCACGCGGGTCGGCAGCGGTCCGTTCCCCACTGAACTCCTTGACGCCGATGGCGAGAAACTGCGGCAGG
TTGGCCACGAATTCGGCGCAACCACCGGGCGGCCGCGCCGCTGCGGCTGGTTCGACGCCATGGTGGTCCGCTACGCGGTA
CGGGTCAATGGCCTCACCGGCGTGGCACTGACAAAGCTCGATGTCCTGAACGACTTCGAGACCATCAAAGTCTGCACCGG
CTACACCTTCGAAGGCAAACCCTTGGCCGACCTCCCCGCCAACCTCGCCGTGTTCGAAAAGTGCGAACCGGTCTACGAGG
AGCTGCCGGGCTGGATGAGCGACATCAGCGGTGCGCGCACCTTCGAAGAGCTGCCCGAGAAGGCGAAAAGCTACGTGAAG
CGCCTCGAGCAACTGATCGGCTGCCCCATCGTCCTCGTCTCCATCGGGCCGCGCCGGGACCAGACCATAATCATCAGCAA
CCCGTTTCAGGATAAAGTGTAA

Upstream 100 bases:

>100_bases
CCTCGTTCGTGTTGCAGACGGACAGGGACAACGGATAAAGAAGGCAGAGGTGTTCAGCGAACGGTTTTCGCTGGACGCCG
GACCTGACAAGGAGTCTTAA

Downstream 100 bases:

>100_bases
AAATTACTTGACCGACTGCAAAGACTCTGCTAAAACTTGTCTTCTCTTTGCGAGCCGCTAGCTCAGATGGTAGAGCACCT
GACTTTTAATCAGGTGGTCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVGEEKVVLHLIPSGILHEGKRCIIGNGVVLDPEVFI
QEITRLKDKGRLKDDRALLVSESIHIIMPYHKRIDIAREAKSGEKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDRDVFAR
KLKENLEEKNVILEKLLGDKPFTFEEIYEQYCGYADILRNYVADTSLILYNDSKAGKKLLFEGAQGTLLDVDHGTYPFVT
SSSTCAGGACTGTGVSPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEKLRQVGHEFGATTGRPRRCGWFDAMVVRYAV
RVNGLTGVALTKLDVLNDFETIKVCTGYTFEGKPLADLPANLAVFEKCEPVYEELPGWMSDISGARTFEELPEKAKSYVK
RLEQLIGCPIVLVSIGPRRDQTIIISNPFQDKV

Sequences:

>Translated_433_residues
MANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVGEEKVVLHLIPSGILHEGKRCIIGNGVVLDPEVFI
QEITRLKDKGRLKDDRALLVSESIHIIMPYHKRIDIAREAKSGEKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDRDVFAR
KLKENLEEKNVILEKLLGDKPFTFEEIYEQYCGYADILRNYVADTSLILYNDSKAGKKLLFEGAQGTLLDVDHGTYPFVT
SSSTCAGGACTGTGVSPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEKLRQVGHEFGATTGRPRRCGWFDAMVVRYAV
RVNGLTGVALTKLDVLNDFETIKVCTGYTFEGKPLADLPANLAVFEKCEPVYEELPGWMSDISGARTFEELPEKAKSYVK
RLEQLIGCPIVLVSIGPRRDQTIIISNPFQDKV
>Mature_432_residues
ANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVGEEKVVLHLIPSGILHEGKRCIIGNGVVLDPEVFIQ
EITRLKDKGRLKDDRALLVSESIHIIMPYHKRIDIAREAKSGEKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDRDVFARK
LKENLEEKNVILEKLLGDKPFTFEEIYEQYCGYADILRNYVADTSLILYNDSKAGKKLLFEGAQGTLLDVDHGTYPFVTS
SSTCAGGACTGTGVSPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEKLRQVGHEFGATTGRPRRCGWFDAMVVRYAVR
VNGLTGVALTKLDVLNDFETIKVCTGYTFEGKPLADLPANLAVFEKCEPVYEELPGWMSDISGARTFEELPEKAKSYVKR
LEQLIGCPIVLVSIGPRRDQTIIISNPFQDKV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=43.7647058823529, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=43.6619718309859, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=41.9191919191919, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=51.6355140186916, Blast_Score=440, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=43.953488372093, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=43.953488372093, Blast_Score=350, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=44.367816091954, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.9928741092637, Blast_Score=335, Evalue=2e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_GEOSL (Q747F9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954348.1
- ProteinModelPortal:   Q747F9
- SMR:   Q747F9
- GeneID:   2686657
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3308
- NMPDR:   fig|243231.1.peg.3286
- TIGR:   GSU3308
- HOGENOM:   HBG658237
- OMA:   KRGRLQQ
- ProtClustDB:   PRK01117
- BioCyc:   GSUL243231:GSU_3308-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47786; Mature: 47655

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVGEEKVVLHLIPSGIL
CCCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEECCCEEEEEECCCHHH
HEGKRCIIGNGVVLDPEVFIQEITRLKDKGRLKDDRALLVSESIHIIMPYHKRIDIAREA
HCCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHHHHH
KSGEKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDRDVFARKLKENLEEKNVILEKLLGDK
CCCCHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFTFEEIYEQYCGYADILRNYVADTSLILYNDSKAGKKLLFEGAQGTLLDVDHGTYPFVT
CCCHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCHHEEECCCCCEEECCCCCCCEEE
SSSTCAGGACTGTGVSPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEKLRQVGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHCC
TTGRPRRCGWFDAMVVRYAVRVNGLTGVALTKLDVLNDFETIKVCTGYTFEGKPLADLPA
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCCC
NLAVFEKCEPVYEELPGWMSDISGARTFEELPEKAKSYVKRLEQLIGCPIVLVSIGPRRD
CHHHHHHCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
QTIIISNPFQDKV
CEEEEECCCCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVGEEKVVLHLIPSGIL
CCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEECCCEEEEEECCCHHH
HEGKRCIIGNGVVLDPEVFIQEITRLKDKGRLKDDRALLVSESIHIIMPYHKRIDIAREA
HCCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHHHHH
KSGEKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDRDVFARKLKENLEEKNVILEKLLGDK
CCCCHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFTFEEIYEQYCGYADILRNYVADTSLILYNDSKAGKKLLFEGAQGTLLDVDHGTYPFVT
CCCHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCHHEEECCCCCEEECCCCCCCEEE
SSSTCAGGACTGTGVSPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEKLRQVGHEFGA
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHCC
TTGRPRRCGWFDAMVVRYAVRVNGLTGVALTKLDVLNDFETIKVCTGYTFEGKPLADLPA
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHCCCC
NLAVFEKCEPVYEELPGWMSDISGARTFEELPEKAKSYVKRLEQLIGCPIVLVSIGPRRD
CHHHHHHCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
QTIIISNPFQDKV
CEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA