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The map label for this gene is hisZ

Identifier: 39998396

GI number: 39998396

Start: 3631136

End: 3632452

Strand: Direct

Name: hisZ

Synonym: GSU3307

Alternate gene names: 39998396

Gene position: 3631136-3632452 (Clockwise)

Preceding gene: 39998395

Following gene: 39998397

Centisome position: 95.2

GC content: 60.97

Gene sequence:

>1317_bases
GTGACTCCGATAACCCCCATAGAAGCCCCCCTTCCCAAGGGGGTTACAGACTTTCTCCCTGAAAAAGCCGACAAGATCGG
CTACATCGAGGGCAAAATCCGCAAAGTCTTCGAACTCTGGGGGTTCCGGCGGATCATCACGCCGCTCCTGGAGTTTGAAG
ACGTCATTGCCGCCGGACTCGGCGACGACCTGAAGGCAAAAACCTTTCGCTTCGATGACCGCCAAAGCGGCAAACTGATT
GCCGTTCCTTCCGACATCACTCCCCAGATTGCCCGCATCGTAGCAACTCGGCTGAGAGGCTACCCCCTGCCCCACCGCAT
CTGCTACAGCGGTAGGGTGTTGCGTCACGCGGAACTCCAGTCGGGCCGGAGCCGCGAGATCTTCCAGTCGGGCGTAGAAC
TCATCGGCCTCGACTCACCCGAAGCGGACGCCGAAATGGTGACCATGGCGGTGGAAGCCCTCAAGGGACTCGGGTTCAGA
GACTTCAAGATCGACCTGGGACACGTGGGATTCATTCGGGGAATCATGACGGCATCCGGGCTCGAAGTGGCGGTACGCAA
CCGGCTTCAGGAGGCCATCGGCAAGAAGGACGTGTCTGCGGTCAGGTCGATCCTGGCGGAGTCGCCCCTCTCTGATGCGG
CAAAGGATGAACTGGCCGCCCTTCCCCGCCTGTTCGGCGGCCGCGAGGTGCTGGACGAAGCCGGACGCGTGGCGACCAAC
GACACGTCGCGCCGGGCACTGGACAACATCTCGCAGGTTCTGGACTTACTCGATATTCATGGAGTTTCAGATCATTTGAC
CATTGATCTGGGCGAAGTTCGCGGGCTTGACTACCATACCGGCCTCACCTTCGAAGGCTTCGTGACCGGCATGGGCGAGG
CCGTCTGCAGCGGCGGCCGCTACGACACGCTCACGGCACGCTACGGTTTTCCGGCGCCGGCCACGGGCTTCACCTTCAAC
GTTCTGGCGCTCCTGTCGGCCCTTGAGAAAAGGCCCGACGTGGAAGCGAGCAAGACCCGGGACATCCTCATCTTCAATCA
GCAGGACGACCGGCGCGAGGCGCTCGAAATCGCCCAGCAGCTGCGGCGCCGGGGATACACCACCGCCCGCGACATCATAC
GCAGAAATTTTGACGATTCGCTTGACTACGCGCGCCGCATGAACATCCTTCACATGATGGTGGTCGGCGGCGATCAGTGC
GGGCCGGACGAGGTCTACCTCGTTCGTGTTGCAGACGGACAGGGACAACGGATAAAGAAGGCAGAGGTGTTCAGCGAACG
GTTTTCGCTGGACGCCGGACCTGACAAGGAGTCTTAA

Upstream 100 bases:

>100_bases
GCGGGGATATTATCATTGACATGACCCAGCTCAACTGCTAGGATTTTCGGGTTTTTCGTCCACTTTCCCCTCACGGGCCC
CGACAGACAGGTCAACACAC

Downstream 100 bases:

>100_bases
ATGGCTAACGTGGTTGTGGTTGGTGCCCAGTGGGGCGACGAAGGCAAGGGCAAGGTCGTTGACATCTATACCGAGCATGC
GGACGACGTGGTCCGTTACC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTPITPIEAPLPKGVTDFLPEKADKIGYIEGKIRKVFELWGFRRIITPLLEFEDVIAAGLGDDLKAKTFRFDDRQSGKLI
AVPSDITPQIARIVATRLRGYPLPHRICYSGRVLRHAELQSGRSREIFQSGVELIGLDSPEADAEMVTMAVEALKGLGFR
DFKIDLGHVGFIRGIMTASGLEVAVRNRLQEAIGKKDVSAVRSILAESPLSDAAKDELAALPRLFGGREVLDEAGRVATN
DTSRRALDNISQVLDLLDIHGVSDHLTIDLGEVRGLDYHTGLTFEGFVTGMGEAVCSGGRYDTLTARYGFPAPATGFTFN
VLALLSALEKRPDVEASKTRDILIFNQQDDRREALEIAQQLRRRGYTTARDIIRRNFDDSLDYARRMNILHMMVVGGDQC
GPDEVYLVRVADGQGQRIKKAEVFSERFSLDAGPDKES

Sequences:

>Translated_438_residues
MTPITPIEAPLPKGVTDFLPEKADKIGYIEGKIRKVFELWGFRRIITPLLEFEDVIAAGLGDDLKAKTFRFDDRQSGKLI
AVPSDITPQIARIVATRLRGYPLPHRICYSGRVLRHAELQSGRSREIFQSGVELIGLDSPEADAEMVTMAVEALKGLGFR
DFKIDLGHVGFIRGIMTASGLEVAVRNRLQEAIGKKDVSAVRSILAESPLSDAAKDELAALPRLFGGREVLDEAGRVATN
DTSRRALDNISQVLDLLDIHGVSDHLTIDLGEVRGLDYHTGLTFEGFVTGMGEAVCSGGRYDTLTARYGFPAPATGFTFN
VLALLSALEKRPDVEASKTRDILIFNQQDDRREALEIAQQLRRRGYTTARDIIRRNFDDSLDYARRMNILHMMVVGGDQC
GPDEVYLVRVADGQGQRIKKAEVFSERFSLDAGPDKES
>Mature_437_residues
TPITPIEAPLPKGVTDFLPEKADKIGYIEGKIRKVFELWGFRRIITPLLEFEDVIAAGLGDDLKAKTFRFDDRQSGKLIA
VPSDITPQIARIVATRLRGYPLPHRICYSGRVLRHAELQSGRSREIFQSGVELIGLDSPEADAEMVTMAVEALKGLGFRD
FKIDLGHVGFIRGIMTASGLEVAVRNRLQEAIGKKDVSAVRSILAESPLSDAAKDELAALPRLFGGREVLDEAGRVATND
TSRRALDNISQVLDLLDIHGVSDHLTIDLGEVRGLDYHTGLTFEGFVTGMGEAVCSGGRYDTLTARYGFPAPATGFTFNV
LALLSALEKRPDVEASKTRDILIFNQQDDRREALEIAQQLRRRGYTTARDIIRRNFDDSLDYARRMNILHMMVVGGDQCG
PDEVYLVRVADGQGQRIKKAEVFSERFSLDAGPDKES

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI15029520, Length=449, Percent_Identity=23.162583518931, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI6996014, Length=420, Percent_Identity=22.6190476190476, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1788861, Length=364, Percent_Identity=25.5494505494505, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71993693, Length=447, Percent_Identity=22.8187919463087, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71993686, Length=447, Percent_Identity=22.8187919463087, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6325290, Length=363, Percent_Identity=25.6198347107438, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24643061, Length=365, Percent_Identity=27.1232876712329, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643059, Length=388, Percent_Identity=25.5154639175258, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_GEOSL (P60837)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954347.1
- ProteinModelPortal:   P60837
- GeneID:   2688239
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3307
- NMPDR:   fig|243231.1.peg.3285
- TIGR:   GSU3307
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12292
- BioCyc:   GSUL243231:GSU_3307-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 48418; Mature: 48287

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPITPIEAPLPKGVTDFLPEKADKIGYIEGKIRKVFELWGFRRIITPLLEFEDVIAAGL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDDLKAKTFRFDDRQSGKLIAVPSDITPQIARIVATRLRGYPLPHRICYSGRVLRHAELQ
CCCCCHHEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHH
SGRSREIFQSGVELIGLDSPEADAEMVTMAVEALKGLGFRDFKIDLGHVGFIRGIMTASG
CCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
LEVAVRNRLQEAIGKKDVSAVRSILAESPLSDAAKDELAALPRLFGGREVLDEAGRVATN
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
DTSRRALDNISQVLDLLDIHGVSDHLTIDLGEVRGLDYHTGLTFEGFVTGMGEAVCSGGR
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
YDTLTARYGFPAPATGFTFNVLALLSALEKRPDVEASKTRDILIFNQQDDRREALEIAQQ
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
LRRRGYTTARDIIRRNFDDSLDYARRMNILHMMVVGGDQCGPDEVYLVRVADGQGQRIKK
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCCHHHH
AEVFSERFSLDAGPDKES
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPITPIEAPLPKGVTDFLPEKADKIGYIEGKIRKVFELWGFRRIITPLLEFEDVIAAGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDDLKAKTFRFDDRQSGKLIAVPSDITPQIARIVATRLRGYPLPHRICYSGRVLRHAELQ
CCCCCHHEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHH
SGRSREIFQSGVELIGLDSPEADAEMVTMAVEALKGLGFRDFKIDLGHVGFIRGIMTASG
CCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
LEVAVRNRLQEAIGKKDVSAVRSILAESPLSDAAKDELAALPRLFGGREVLDEAGRVATN
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
DTSRRALDNISQVLDLLDIHGVSDHLTIDLGEVRGLDYHTGLTFEGFVTGMGEAVCSGGR
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
YDTLTARYGFPAPATGFTFNVLALLSALEKRPDVEASKTRDILIFNQQDDRREALEIAQQ
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
LRRRGYTTARDIIRRNFDDSLDYARRMNILHMMVVGGDQCGPDEVYLVRVADGQGQRIKK
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEECCCCCHHHH
AEVFSERFSLDAGPDKES
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA