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The map label for this gene is hemA

Identifier: 39998373

GI number: 39998373

Start: 3602688

End: 3603992

Strand: Direct

Name: hemA

Synonym: GSU3284

Alternate gene names: 39998373

Gene position: 3602688-3603992 (Clockwise)

Preceding gene: 39998372

Following gene: 39998374

Centisome position: 94.46

GC content: 60.38

Gene sequence:

>1305_bases
ATGAACATTGTTGTGGTGGGGCTCTCGCACAAGACAGCCTCCGTGGAAATTCGCGAGAAAATCGCTTTTGCCCCGACCCA
GATGGAAAAGCCCCTGCGCATGCTCATCGCCATTGACGACATCGCCGAGGCGGTGATCGTCTCCACCTGTAACCGGGTCG
AGCTCTACGCGTCGACCCGAGACGTGGCCGGCGGCATGGCGCGGCTCAAGCGCTTCCTGGGTGACTATCACGGTGTCCCC
GTCGAGGTTCTTGAGCCCCACCTGTACAGTCATCACGGCGAGGCGGCCATTCGTCACGTGTTCCGGGTAGCGGCAAGCCT
TGACTCCATGGTCGTGGGCGAACCCCAGATTCTCGGCCAGATCAAGACCGCCTACGGTTACGCCGCGGAGTTCAGGACTT
CGGGCATCATCCTCAACCGCTTCCTGCACAAGGCATTTTCCGTGGCCAAACGGGTCCGTACCGAGACGAAGATTGCTTCG
TCGGCCGTTTCGGTCTCATTTGCCGCCGTGGAGCTGGCCCGCAAGATATTCGGCGACCTTTCCGACAAGACCGTCATGCT
CATCGGTGCCGGTGAAATGTGCGAACTGGCGGCGAAACACTTCCTGAACAACGGTGCGCGCGGCGTCATGGTCACCAACC
GGACCTACGAGCGGGCCGAACGGCTCGCCGAAGAGTTCGAGGGAAAAGCGATCCACTTCGAGGATCTCTTCGACCAGCTC
CACAAGGCGGACATCGTCCTCTCCTCCACGGGCGCCACCCACTACATCATCCGGCCCAAGGACATCGACGAGGTGATTCG
CCGCCGTAAGATGAAGCCCATGTTTTTCATAGACATCGCCGTTCCCCGGGATATCGACCCCAAGGTGAACGACGTGGAGA
ACGTCTATCTCTACGACATGGACGATCTCCAGAATGTGGTCGCCTCCAACCTCCAGCAACGGGCCGAGGAAGCCAAAAAG
GCGGAAGGCATTATTGAGGAGGAGATCGGCCAGTTCTACAAATGGATATCAAGCCTTGAGGTCACGCCGACCATCGTGGC
CCTGCGCTCCAAATTCGAGGATGTCCGTCGGGCCGAACTGGAAAAGACCCTCTCGGCCTGGAAAGACCTGCCCCCCGACG
GGGCTAAGCGGCTGGAGGCACTCACTGCCGCCATCGTCAACAAGCTCCTCCACCCTCCCACCGCCACTCTCAAGCGAACC
GGCCAGGGGGGGCGAACCGATCTTTATGTGGACGCCCTGCGCACCCTCTTCGATCTCCAGACGGAGCTTCCGGAGCCCGA
AGGAAGCCTGGAGCTTGAGGAGTGA

Upstream 100 bases:

>100_bases
CGCCATCATCGGTTTTTTCGTGCTCCTCTTTACCTTTCTCGGCGTGAACCTTTTCATGTCGGGGCTGCACAGCTACACGT
GAGAAAAGAGGCATACTGAT

Downstream 100 bases:

>100_bases
CCCGCTGCTGCATGTATGTTCTACCCCATTAACGAGACAGTAAGGAGACCGGAAACCTATGGCACTTAACCGCCTCAGAA
TCGGAACCCGGGCGAGCCAG

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNIVVVGLSHKTASVEIREKIAFAPTQMEKPLRMLIAIDDIAEAVIVSTCNRVELYASTRDVAGGMARLKRFLGDYHGVP
VEVLEPHLYSHHGEAAIRHVFRVAASLDSMVVGEPQILGQIKTAYGYAAEFRTSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVSFAAVELARKIFGDLSDKTVMLIGAGEMCELAAKHFLNNGARGVMVTNRTYERAERLAEEFEGKAIHFEDLFDQL
HKADIVLSSTGATHYIIRPKDIDEVIRRRKMKPMFFIDIAVPRDIDPKVNDVENVYLYDMDDLQNVVASNLQQRAEEAKK
AEGIIEEEIGQFYKWISSLEVTPTIVALRSKFEDVRRAELEKTLSAWKDLPPDGAKRLEALTAAIVNKLLHPPTATLKRT
GQGGRTDLYVDALRTLFDLQTELPEPEGSLELEE

Sequences:

>Translated_434_residues
MNIVVVGLSHKTASVEIREKIAFAPTQMEKPLRMLIAIDDIAEAVIVSTCNRVELYASTRDVAGGMARLKRFLGDYHGVP
VEVLEPHLYSHHGEAAIRHVFRVAASLDSMVVGEPQILGQIKTAYGYAAEFRTSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVSFAAVELARKIFGDLSDKTVMLIGAGEMCELAAKHFLNNGARGVMVTNRTYERAERLAEEFEGKAIHFEDLFDQL
HKADIVLSSTGATHYIIRPKDIDEVIRRRKMKPMFFIDIAVPRDIDPKVNDVENVYLYDMDDLQNVVASNLQQRAEEAKK
AEGIIEEEIGQFYKWISSLEVTPTIVALRSKFEDVRRAELEKTLSAWKDLPPDGAKRLEALTAAIVNKLLHPPTATLKRT
GQGGRTDLYVDALRTLFDLQTELPEPEGSLELEE
>Mature_434_residues
MNIVVVGLSHKTASVEIREKIAFAPTQMEKPLRMLIAIDDIAEAVIVSTCNRVELYASTRDVAGGMARLKRFLGDYHGVP
VEVLEPHLYSHHGEAAIRHVFRVAASLDSMVVGEPQILGQIKTAYGYAAEFRTSGIILNRFLHKAFSVAKRVRTETKIAS
SAVSVSFAAVELARKIFGDLSDKTVMLIGAGEMCELAAKHFLNNGARGVMVTNRTYERAERLAEEFEGKAIHFEDLFDQL
HKADIVLSSTGATHYIIRPKDIDEVIRRRKMKPMFFIDIAVPRDIDPKVNDVENVYLYDMDDLQNVVASNLQQRAEEAKK
AEGIIEEEIGQFYKWISSLEVTPTIVALRSKFEDVRRAELEKTLSAWKDLPPDGAKRLEALTAAIVNKLLHPPTATLKRT
GQGGRTDLYVDALRTLFDLQTELPEPEGSLELEE

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=416, Percent_Identity=40.625, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_GEOSL (Q747I2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954324.1
- ProteinModelPortal:   Q747I2
- SMR:   Q747I2
- GeneID:   2688265
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3284
- NMPDR:   fig|243231.1.peg.3262
- TIGR:   GSU3284
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   GSUL243231:GSU_3284-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 48487; Mature: 48487

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVVVGLSHKTASVEIREKIAFAPTQMEKPLRMLIAIDDIAEAVIVSTCNRVELYASTR
CCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHH
DVAGGMARLKRFLGDYHGVPVEVLEPHLYSHHGEAAIRHVFRVAASLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTAYGYAAEFRTSGIILNRFLHKAFSVAKRVRTETKIASSAVSVSFAAVELARKIFGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDKTVMLIGAGEMCELAAKHFLNNGARGVMVTNRTYERAERLAEEFEGKAIHFEDLFDQL
CCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHH
HKADIVLSSTGATHYIIRPKDIDEVIRRRKMKPMFFIDIAVPRDIDPKVNDVENVYLYDM
HHHCEEEECCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEH
DDLQNVVASNLQQRAEEAKKAEGIIEEEIGQFYKWISSLEVTPTIVALRSKFEDVRRAEL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EKTLSAWKDLPPDGAKRLEALTAAIVNKLLHPPTATLKRTGQGGRTDLYVDALRTLFDLQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHH
TELPEPEGSLELEE
HHCCCCCCCCCCCC
>Mature Secondary Structure
MNIVVVGLSHKTASVEIREKIAFAPTQMEKPLRMLIAIDDIAEAVIVSTCNRVELYASTR
CCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHH
DVAGGMARLKRFLGDYHGVPVEVLEPHLYSHHGEAAIRHVFRVAASLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHCCCCHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTAYGYAAEFRTSGIILNRFLHKAFSVAKRVRTETKIASSAVSVSFAAVELARKIFGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDKTVMLIGAGEMCELAAKHFLNNGARGVMVTNRTYERAERLAEEFEGKAIHFEDLFDQL
CCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHH
HKADIVLSSTGATHYIIRPKDIDEVIRRRKMKPMFFIDIAVPRDIDPKVNDVENVYLYDM
HHHCEEEECCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEH
DDLQNVVASNLQQRAEEAKKAEGIIEEEIGQFYKWISSLEVTPTIVALRSKFEDVRRAEL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EKTLSAWKDLPPDGAKRLEALTAAIVNKLLHPPTATLKRTGQGGRTDLYVDALRTLFDLQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHH
TELPEPEGSLELEE
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA