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The map label for this gene is hemC

Identifier: 39998374

GI number: 39998374

Start: 3604051

End: 3605007

Strand: Direct

Name: hemC

Synonym: GSU3285

Alternate gene names: 39998374

Gene position: 3604051-3605007 (Clockwise)

Preceding gene: 39998373

Following gene: 39998375

Centisome position: 94.49

GC content: 62.9

Gene sequence:

>957_bases
ATGGCACTTAACCGCCTCAGAATCGGAACCCGGGCGAGCCAGCTCGCTCTCTGGCAGGCCAATTGGGTCAAGTCCGAGCT
GGAAAAACGCTACCCCGGCATGGAGGTCGAGCTGGTCAAGATCAAGACCATCGGCGACAAGATCCTTGACGTCCCCCTGG
CCCAGGTGGGTGGCAAAGGACTCTTCGTGAAAGAGATCGAGGAGGCGATGCTGAGGGGCGAAATCGACATCGCCGTGCAC
AGCATGAAGGACGTGCCGACCGAGTTTCCCGAAGGCCTCGGACTGGTCTGCATCACCGAGCGTGAAGACCCTCGTGATGC
TTTCATTTCCAACGGCGTGACCTTCGCCAATCTGCCCCAGGGGGCGAAAATCGGTACATCGGCGCTGCGCCGCCAGGCCC
AGCTCCTCAAGGTCCGTCCCGACCTCGAAATGGTAATAATCCGCGGCAACGTGGAGACGCGTATCCGGAAGCTCACCGAG
GACAAGCTGGACGCCGTCATCCTGGCTGCCGCAGGCCTCAAGCGCCTCGGATTCACCGACGTGGTAACCGAATATCTGCC
CGTTGATCTCTCCCTGCCGGCTATCGGCCAGGGCGCCCTGGGCCTGGAGTGCCGCCTCGACGACCAGGCCGTCCGCGAAA
CCATCGACTTTTTCAACCACCCTGACACTGCACACGCGGTACGGGCCGAGCGGGCACTGCTCTGGCGCTGCGAGGGGGGA
TGCCAGGTTCCCATTGCCGCCCACGGCCAGGTGAGCGGAGACTCCCTGACGCTGACCGGCTTTATCGCCTCCGTGGATGG
CACCCGTTCCGTCAAGGAGAGCATCTCAGGGCCGGTGACCGACTGCGAGAAGCTGGGCATTGCCCTGGCCGAGAAACTCC
TCGCCGACGGCGGGCACGAGATTCTTGCCGAGGTCTACCAGCGGGAAGTGTCCCGGGAGAAGGAAATCCCGGTTTAA

Upstream 100 bases:

>100_bases
GAGCTTCCGGAGCCCGAAGGAAGCCTGGAGCTTGAGGAGTGACCCGCTGCTGCATGTATGTTCTACCCCATTAACGAGAC
AGTAAGGAGACCGGAAACCT

Downstream 100 bases:

>100_bases
TCGTCCATCCGCTTCACCGTCTCCGGCCACAGGGGCATGGAGGAATTCAGAGGAGGCCTGCCGGACCTTCTTCCTCTGAA
ATCCTCTGTGCCCCCTGTGG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MALNRLRIGTRASQLALWQANWVKSELEKRYPGMEVELVKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVH
SMKDVPTEFPEGLGLVCITEREDPRDAFISNGVTFANLPQGAKIGTSALRRQAQLLKVRPDLEMVIIRGNVETRIRKLTE
DKLDAVILAAAGLKRLGFTDVVTEYLPVDLSLPAIGQGALGLECRLDDQAVRETIDFFNHPDTAHAVRAERALLWRCEGG
CQVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLLADGGHEILAEVYQREVSREKEIPV

Sequences:

>Translated_318_residues
MALNRLRIGTRASQLALWQANWVKSELEKRYPGMEVELVKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVH
SMKDVPTEFPEGLGLVCITEREDPRDAFISNGVTFANLPQGAKIGTSALRRQAQLLKVRPDLEMVIIRGNVETRIRKLTE
DKLDAVILAAAGLKRLGFTDVVTEYLPVDLSLPAIGQGALGLECRLDDQAVRETIDFFNHPDTAHAVRAERALLWRCEGG
CQVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLLADGGHEILAEVYQREVSREKEIPV
>Mature_317_residues
ALNRLRIGTRASQLALWQANWVKSELEKRYPGMEVELVKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVHS
MKDVPTEFPEGLGLVCITEREDPRDAFISNGVTFANLPQGAKIGTSALRRQAQLLKVRPDLEMVIIRGNVETRIRKLTED
KLDAVILAAAGLKRLGFTDVVTEYLPVDLSLPAIGQGALGLECRLDDQAVRETIDFFNHPDTAHAVRAERALLWRCEGGC
QVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLLADGGHEILAEVYQREVSREKEIPV

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=331, Percent_Identity=39.8791540785499, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI66933009, Length=331, Percent_Identity=39.8791540785499, Blast_Score=235, Evalue=3e-62,
Organism=Escherichia coli, GI48994974, Length=308, Percent_Identity=55.1948051948052, Blast_Score=326, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6319996, Length=321, Percent_Identity=36.4485981308411, Blast_Score=199, Evalue=3e-52,
Organism=Drosophila melanogaster, GI20130425, Length=287, Percent_Identity=39.0243902439024, Blast_Score=201, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_GEOSL (Q747I1)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954325.1
- ProteinModelPortal:   Q747I1
- SMR:   Q747I1
- GeneID:   2685563
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3285
- NMPDR:   fig|243231.1.peg.3263
- TIGR:   GSU3285
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   GSUL243231:GSU_3285-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34708; Mature: 34576

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNRLRIGTRASQLALWQANWVKSELEKRYPGMEVELVKIKTIGDKILDVPLAQVGGKG
CCCCCEECCCCHHHEEEHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHCCHHHHCCCC
LFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLVCITEREDPRDAFISNGVTFANLPQ
EEHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCCC
GAKIGTSALRRQAQLLKVRPDLEMVIIRGNVETRIRKLTEDKLDAVILAAAGLKRLGFTD
CCCCCHHHHHHHHHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VVTEYLPVDLSLPAIGQGALGLECRLDDQAVRETIDFFNHPDTAHAVRAERALLWRCEGG
HHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCC
CQVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLLADGGHE
CEEEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
ILAEVYQREVSREKEIPV
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALNRLRIGTRASQLALWQANWVKSELEKRYPGMEVELVKIKTIGDKILDVPLAQVGGKG
CCCCEECCCCHHHEEEHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHCCHHHHCCCC
LFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLVCITEREDPRDAFISNGVTFANLPQ
EEHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCCC
GAKIGTSALRRQAQLLKVRPDLEMVIIRGNVETRIRKLTEDKLDAVILAAAGLKRLGFTD
CCCCCHHHHHHHHHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VVTEYLPVDLSLPAIGQGALGLECRLDDQAVRETIDFFNHPDTAHAVRAERALLWRCEGG
HHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCC
CQVPIAAHGQVSGDSLTLTGFIASVDGTRSVKESISGPVTDCEKLGIALAEKLLADGGHE
CEEEEEECCCCCCCCEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
ILAEVYQREVSREKEIPV
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA