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The map label for this gene is zraS [H]

Identifier: 39998342

GI number: 39998342

Start: 3564114

End: 3565757

Strand: Direct

Name: zraS [H]

Synonym: GSU3252

Alternate gene names: 39998342

Gene position: 3564114-3565757 (Clockwise)

Preceding gene: 39998340

Following gene: 39998343

Centisome position: 93.44

GC content: 60.16

Gene sequence:

>1644_bases
GTGGAGTGGAAGTGCTGCTGGAACAGAAACGTTATTGAGCCGGCCGATTGCCCAAACATCGGCGAAGGCGAGGAAATCCT
CTTTTCATCCCTCCAACGCAGAGTGATCGAAAAATGCGTGGATTGCCCGCTGTTCGCCGAAGACCTGAAGGCACTGCGCG
ACTCGGGGCACCCCCTCGCGGCAGTGCTCCCCATTGTGGTGGCTGAGCTCCAGGAACAGCGGAGCCGACTCCAGTCCATG
GTCGGCTTCCTGGACAGCAAGGATCGGGAAATCAAGTTCCTGCACGAAATCAGCCTCGTGCTCCAGACCTCGGTGGACCT
GGATGAAGTGCTGTCGGTGGTCATGACCGCCATCACCGCCGGCAAGGGGTTCGGCATGAACCGGGCCTTTCTGCTGCTGA
CCGACAAGGAGCGCAAGCACCTGCGGGGCTATCTGGGGGTTGGGCCGCGGAACTACGGCGAGGCGTGGTTCATCTGGGAT
GAGATCAGCCGCGACGACTTCACCCTCAAGGAGATGGCGAAGAACTTCTACCAGACCAAATTCACCAGTGAGAAGCAGAA
GTTTCACGACATTCTGGAAAACCTCACCATCCCCTTGTCGGAACATGATCACATCTTGGTCCGTGCGTTGAGGGAGAAGA
AACCGCTCCTGGTCGAAAACGCTTTCCACAATCCAGACTGCGACTACTCCCTGACCCAGACCCTGGGGGTCGACACCTTT
CTCATCACGCCGCTGGTCTCGCGCAACCGGAGAATCGGCGTCATCATTTCCGACAACTGCGTGACCCATAAACCGATTAC
CCCCCAGGATGTCCAGTCCCTGGAGACCTTCGCCTTTCCCGTTGCCTTCGCCATCGAACGGGCATCCCTGTACGAACGGC
TCCAGGAAGAGCTCCTCAAGGTGACGGCGGCCAACGTCAAGCTCAAGGAACAGCAGGAGCTGATCGTCCGGATGGAACGG
ATGGCGCTCGTGGGCAAGATCACGTCGAGCATCGCCCACTCCATCCGCAACCCCCTCATGATCATCGGCGGCTTCGCCCG
CACGCTTCTCAAGGGCACCGATGAGGGTGACCCCCGGCGCGAGTATCTGGAATCCATCGTCCGCGAAGCCCGACAGTTGG
ACGAGGTGCTGGACGAAGTACTCAGCTACTCCGACTCCCTCTATCCCACCCTGGACTCATGGGACGTGAACCAGTTGGTA
AGCACCGGTTGCCGGGAGCTGGACAGCCGCCTCCAGGAGCGTCGCATTGCCTGCCGGCTCCGCCTGGACGCCGAACTCCC
GTCCGCCCGCATCGATTACAAACAGGTCTCCTTCTGCCTCCGCTCAATCCTGACCTCCAGCATGGACCGAATGCCCGACG
GCGGTGAGATTGTCATCGAAACCCGACTGGAAGGCGGGTGGATCGTCGTCGAGGTGCGGGACACGGGACGGCCCGGCTCC
TCGAACGGAATGAGCACATTCTCTTCCTTCGCACCGCCCCAGGAACTGGGAGGTGGCCTCGGCCTGGAAGTCTGCAAGGC
AATCATGGAAAAACATGGCAACAGCCTCATCATCGAAACGCCTCCCGACGGGGGGGCCCGCTACGTGCTCAAACTACCGG
TAAACAGGGAGGAGGAACCCCATGGCACGATTGTTGGTGGTTGA

Upstream 100 bases:

>100_bases
TAAACCGCCGTGATCATTCGGTCATTCGCCTTGAAAATACAGCCCCAACTGGTATAATTGTGCCGCTTCCCACGATAAAA
TCAAGCCCGGGAGATACCCA

Downstream 100 bases:

>100_bases
CGACGAAAACAGCATCCGGCTGCTCTATTCTCAGGAGCTGGCCGAAGAGGGACATGAGGTGGTGACCGCCGCCACGGCAG
CCGAAGCGGTGGACAAGATC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MEWKCCWNRNVIEPADCPNIGEGEEILFSSLQRRVIEKCVDCPLFAEDLKALRDSGHPLAAVLPIVVAELQEQRSRLQSM
VGFLDSKDREIKFLHEISLVLQTSVDLDEVLSVVMTAITAGKGFGMNRAFLLLTDKERKHLRGYLGVGPRNYGEAWFIWD
EISRDDFTLKEMAKNFYQTKFTSEKQKFHDILENLTIPLSEHDHILVRALREKKPLLVENAFHNPDCDYSLTQTLGVDTF
LITPLVSRNRRIGVIISDNCVTHKPITPQDVQSLETFAFPVAFAIERASLYERLQEELLKVTAANVKLKEQQELIVRMER
MALVGKITSSIAHSIRNPLMIIGGFARTLLKGTDEGDPRREYLESIVREARQLDEVLDEVLSYSDSLYPTLDSWDVNQLV
STGCRELDSRLQERRIACRLRLDAELPSARIDYKQVSFCLRSILTSSMDRMPDGGEIVIETRLEGGWIVVEVRDTGRPGS
SNGMSTFSSFAPPQELGGGLGLEVCKAIMEKHGNSLIIETPPDGGARYVLKLPVNREEEPHGTIVGG

Sequences:

>Translated_547_residues
MEWKCCWNRNVIEPADCPNIGEGEEILFSSLQRRVIEKCVDCPLFAEDLKALRDSGHPLAAVLPIVVAELQEQRSRLQSM
VGFLDSKDREIKFLHEISLVLQTSVDLDEVLSVVMTAITAGKGFGMNRAFLLLTDKERKHLRGYLGVGPRNYGEAWFIWD
EISRDDFTLKEMAKNFYQTKFTSEKQKFHDILENLTIPLSEHDHILVRALREKKPLLVENAFHNPDCDYSLTQTLGVDTF
LITPLVSRNRRIGVIISDNCVTHKPITPQDVQSLETFAFPVAFAIERASLYERLQEELLKVTAANVKLKEQQELIVRMER
MALVGKITSSIAHSIRNPLMIIGGFARTLLKGTDEGDPRREYLESIVREARQLDEVLDEVLSYSDSLYPTLDSWDVNQLV
STGCRELDSRLQERRIACRLRLDAELPSARIDYKQVSFCLRSILTSSMDRMPDGGEIVIETRLEGGWIVVEVRDTGRPGS
SNGMSTFSSFAPPQELGGGLGLEVCKAIMEKHGNSLIIETPPDGGARYVLKLPVNREEEPHGTIVGG
>Mature_547_residues
MEWKCCWNRNVIEPADCPNIGEGEEILFSSLQRRVIEKCVDCPLFAEDLKALRDSGHPLAAVLPIVVAELQEQRSRLQSM
VGFLDSKDREIKFLHEISLVLQTSVDLDEVLSVVMTAITAGKGFGMNRAFLLLTDKERKHLRGYLGVGPRNYGEAWFIWD
EISRDDFTLKEMAKNFYQTKFTSEKQKFHDILENLTIPLSEHDHILVRALREKKPLLVENAFHNPDCDYSLTQTLGVDTF
LITPLVSRNRRIGVIISDNCVTHKPITPQDVQSLETFAFPVAFAIERASLYERLQEELLKVTAANVKLKEQQELIVRMER
MALVGKITSSIAHSIRNPLMIIGGFARTLLKGTDEGDPRREYLESIVREARQLDEVLDEVLSYSDSLYPTLDSWDVNQLV
STGCRELDSRLQERRIACRLRLDAELPSARIDYKQVSFCLRSILTSSMDRMPDGGEIVIETRLEGGWIVVEVRDTGRPGS
SNGMSTFSSFAPPQELGGGLGLEVCKAIMEKHGNSLIIETPPDGGARYVLKLPVNREEEPHGTIVGG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=24.4635193133047, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788549, Length=230, Percent_Identity=26.5217391304348, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=331, Percent_Identity=25.0755287009063, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61672; Mature: 61672

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWKCCWNRNVIEPADCPNIGEGEEILFSSLQRRVIEKCVDCPLFAEDLKALRDSGHPLA
CCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHH
AVLPIVVAELQEQRSRLQSMVGFLDSKDREIKFLHEISLVLQTSVDLDEVLSVVMTAITA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GKGFGMNRAFLLLTDKERKHLRGYLGVGPRNYGEAWFIWDEISRDDFTLKEMAKNFYQTK
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
FTSEKQKFHDILENLTIPLSEHDHILVRALREKKPLLVENAFHNPDCDYSLTQTLGVDTF
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCCHHH
LITPLVSRNRRIGVIISDNCVTHKPITPQDVQSLETFAFPVAFAIERASLYERLQEELLK
HHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAANVKLKEQQELIVRMERMALVGKITSSIAHSIRNPLMIIGGFARTLLKGTDEGDPRR
HHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCHHH
EYLESIVREARQLDEVLDEVLSYSDSLYPTLDSWDVNQLVSTGCRELDSRLQERRIACRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
RLDAELPSARIDYKQVSFCLRSILTSSMDRMPDGGEIVIETRLEGGWIVVEVRDTGRPGS
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCCCCC
SNGMSTFSSFAPPQELGGGLGLEVCKAIMEKHGNSLIIETPPDGGARYVLKLPVNREEEP
CCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCC
HGTIVGG
CCCCCCC
>Mature Secondary Structure
MEWKCCWNRNVIEPADCPNIGEGEEILFSSLQRRVIEKCVDCPLFAEDLKALRDSGHPLA
CCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHH
AVLPIVVAELQEQRSRLQSMVGFLDSKDREIKFLHEISLVLQTSVDLDEVLSVVMTAITA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GKGFGMNRAFLLLTDKERKHLRGYLGVGPRNYGEAWFIWDEISRDDFTLKEMAKNFYQTK
CCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
FTSEKQKFHDILENLTIPLSEHDHILVRALREKKPLLVENAFHNPDCDYSLTQTLGVDTF
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCCHHH
LITPLVSRNRRIGVIISDNCVTHKPITPQDVQSLETFAFPVAFAIERASLYERLQEELLK
HHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAANVKLKEQQELIVRMERMALVGKITSSIAHSIRNPLMIIGGFARTLLKGTDEGDPRR
HHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCHHH
EYLESIVREARQLDEVLDEVLSYSDSLYPTLDSWDVNQLVSTGCRELDSRLQERRIACRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
RLDAELPSARIDYKQVSFCLRSILTSSMDRMPDGGEIVIETRLEGGWIVVEVRDTGRPGS
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCCCCC
SNGMSTFSSFAPPQELGGGLGLEVCKAIMEKHGNSLIIETPPDGGARYVLKLPVNREEEP
CCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCC
HGTIVGG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]