Click here to switch to the map view.

The map label for this gene is proB

Identifier: 39998302

GI number: 39998302

Start: 3517870

End: 3518991

Strand: Reverse

Name: proB

Synonym: GSU3212

Alternate gene names: 39998302

Gene position: 3518991-3517870 (Counterclockwise)

Preceding gene: 39998303

Following gene: 39998301

Centisome position: 92.26

GC content: 65.78

Gene sequence:

>1122_bases
ATGCGTAGCGGAATCCTCTCCAAGGTCAAGCGGATCGTCATCAAGATCGGCAGTGGGGTCCTCACCTGTGGGGACAACGG
GCTCAACAAGCCCCTCATGGGCAGCATCGCCGCCCAGGTGGCCGAGCTGCGCGCCAGCGGCCGCCAGGTGATCATCGTCT
CCTCGGGCGCCGTTGCCGCGGGGCGGAAAGAGCTCGGCATCGACGGCAGACCCCGGAGCATTCCCCAGAAGCAGGCGGCG
GCGGCCATCGGCCAGTCGCGCCTCATGCACGCCTACGAGGAGGCCTTCGAGCCCTTCGGCCACAAGGTGGCGCAGATCCT
TCTGACCCGCGACGACCTGGCCCACCGGGGACGGTTTCTCAACGCCCGGGCTACCCTTGATACCCTCCTCTCCTTCGGGG
TGATCCCGATCATCAACGAAAACGACACTGTGGTCTTCGACGAGATCAAGTTCGGCGACAACGACAGCCTCTCCGCCCTG
GTCACCAACCTGGCCGAGGCCAATCTGCTGGTGATCCTGACCGACATCGACGGTTTCTACGAGGCGAATCCCCGTACCAA
CCCCGACGCGCGGCTCATTCCCCTGGTTCGGCAGATCACCCGGGAGATGGAACGGGCTGCCGGCGGCAGCGGTTCCACCG
TCGGCACGGGCGGGATGGTAACCAAGCTGGCTGCGGCCAAGAAGGCCGGCCAGTTCGGGGTGCCGACGCTCATGCTCAAC
GGCAGGAATCCGTCCCTGCTGGCCGAAGCCTTTGCCGGCCGCGAGGTGGGGACCCTGTTCCTCCCCGGCAAGGAGAGCCT
CAACCGGCGCAAGCACTGGATTGCCCATACTCTGAGGCCGTCGGGGAAAATCATCGTGGACGACGGCGCCCGCACGGTCC
TTGCCCGCCAGGGGAAAAGCCTGCTACCGTCGGGGGTGGTCCGGGTCGAGGGGAAGTTCGACCGGGGCGCCTGCGTCCGG
GTCTGCGGCACCGACGGCACTGAGATCGCCCGGGGGCTCGTAGACTATTCCCATGACGAGATCACCCGCATCCTCGGTCA
TCGAAGTGGCGAGATCGAGGCCATCCTCGGCTACAAGTACGGGGACGAGATCATCCACCGGGACAATCTTGTGGTGCTGT
AG

Upstream 100 bases:

>100_bases
TTCCCCTCTCTGCCGCAACCGGCGAGGGGATTTCCCCTTTGCTCGACGAAATCGCCCGCTCCCTCTGGGGCAAGGACGAG
GAGGAGTGGTAGACGGCGCT

Downstream 100 bases:

>100_bases
CCCCCTTTTGTCACAGTTCATCCAAACGTCCCGTAAGGGGGCCGGGGGCTTTCCCGGAGAAAAAACTCCCGCAAAAAGTG
GGGAGCCTGTTCGATGGGAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRSGILSKVKRIVIKIGSGVLTCGDNGLNKPLMGSIAAQVAELRASGRQVIIVSSGAVAAGRKELGIDGRPRSIPQKQAA
AAIGQSRLMHAYEEAFEPFGHKVAQILLTRDDLAHRGRFLNARATLDTLLSFGVIPIINENDTVVFDEIKFGDNDSLSAL
VTNLAEANLLVILTDIDGFYEANPRTNPDARLIPLVRQITREMERAAGGSGSTVGTGGMVTKLAAAKKAGQFGVPTLMLN
GRNPSLLAEAFAGREVGTLFLPGKESLNRRKHWIAHTLRPSGKIIVDDGARTVLARQGKSLLPSGVVRVEGKFDRGACVR
VCGTDGTEIARGLVDYSHDEITRILGHRSGEIEAILGYKYGDEIIHRDNLVVL

Sequences:

>Translated_373_residues
MRSGILSKVKRIVIKIGSGVLTCGDNGLNKPLMGSIAAQVAELRASGRQVIIVSSGAVAAGRKELGIDGRPRSIPQKQAA
AAIGQSRLMHAYEEAFEPFGHKVAQILLTRDDLAHRGRFLNARATLDTLLSFGVIPIINENDTVVFDEIKFGDNDSLSAL
VTNLAEANLLVILTDIDGFYEANPRTNPDARLIPLVRQITREMERAAGGSGSTVGTGGMVTKLAAAKKAGQFGVPTLMLN
GRNPSLLAEAFAGREVGTLFLPGKESLNRRKHWIAHTLRPSGKIIVDDGARTVLARQGKSLLPSGVVRVEGKFDRGACVR
VCGTDGTEIARGLVDYSHDEITRILGHRSGEIEAILGYKYGDEIIHRDNLVVL
>Mature_373_residues
MRSGILSKVKRIVIKIGSGVLTCGDNGLNKPLMGSIAAQVAELRASGRQVIIVSSGAVAAGRKELGIDGRPRSIPQKQAA
AAIGQSRLMHAYEEAFEPFGHKVAQILLTRDDLAHRGRFLNARATLDTLLSFGVIPIINENDTVVFDEIKFGDNDSLSAL
VTNLAEANLLVILTDIDGFYEANPRTNPDARLIPLVRQITREMERAAGGSGSTVGTGGMVTKLAAAKKAGQFGVPTLMLN
GRNPSLLAEAFAGREVGTLFLPGKESLNRRKHWIAHTLRPSGKIIVDDGARTVLARQGKSLLPSGVVRVEGKFDRGACVR
VCGTDGTEIARGLVDYSHDEITRILGHRSGEIEAILGYKYGDEIIHRDNLVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=33.7931034482759, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=34.0277777777778, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=45.9016393442623, Blast_Score=314, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17569967, Length=315, Percent_Identity=36.5079365079365, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17569969, Length=315, Percent_Identity=36.5079365079365, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320506, Length=425, Percent_Identity=38.1176470588235, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=35.6294536817102, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21357643, Length=295, Percent_Identity=35.9322033898305, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_GEOSL (Q747Q3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954253.1
- ProteinModelPortal:   Q747Q3
- GeneID:   2688338
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3212
- NMPDR:   fig|243231.1.peg.3191
- TIGR:   GSU3212
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   GSUL243231:GSU_3212-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39998; Mature: 39998

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSGILSKVKRIVIKIGSGVLTCGDNGLNKPLMGSIAAQVAELRASGRQVIIVSSGAVAA
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GRKELGIDGRPRSIPQKQAAAAIGQSRLMHAYEEAFEPFGHKVAQILLTRDDLAHRGRFL
CCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NARATLDTLLSFGVIPIINENDTVVFDEIKFGDNDSLSALVTNLAEANLLVILTDIDGFY
EHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCE
EANPRTNPDARLIPLVRQITREMERAAGGSGSTVGTGGMVTKLAAAKKAGQFGVPTLMLN
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
GRNPSLLAEAFAGREVGTLFLPGKESLNRRKHWIAHTLRPSGKIIVDDGARTVLARQGKS
CCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCC
LLPSGVVRVEGKFDRGACVRVCGTDGTEIARGLVDYSHDEITRILGHRSGEIEAILGYKY
CCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEECC
GDEIIHRDNLVVL
CCHHEECCCEEEC
>Mature Secondary Structure
MRSGILSKVKRIVIKIGSGVLTCGDNGLNKPLMGSIAAQVAELRASGRQVIIVSSGAVAA
CCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GRKELGIDGRPRSIPQKQAAAAIGQSRLMHAYEEAFEPFGHKVAQILLTRDDLAHRGRFL
CCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
NARATLDTLLSFGVIPIINENDTVVFDEIKFGDNDSLSALVTNLAEANLLVILTDIDGFY
EHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCE
EANPRTNPDARLIPLVRQITREMERAAGGSGSTVGTGGMVTKLAAAKKAGQFGVPTLMLN
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
GRNPSLLAEAFAGREVGTLFLPGKESLNRRKHWIAHTLRPSGKIIVDDGARTVLARQGKS
CCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCC
LLPSGVVRVEGKFDRGACVRVCGTDGTEIARGLVDYSHDEITRILGHRSGEIEAILGYKY
CCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEEECC
GDEIIHRDNLVVL
CCHHEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA