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The map label for this gene is ltaA [H]

Identifier: 39998252

GI number: 39998252

Start: 3468247

End: 3469293

Strand: Direct

Name: ltaA [H]

Synonym: GSU3162

Alternate gene names: 39998252

Gene position: 3468247-3469293 (Clockwise)

Preceding gene: 39998251

Following gene: 39998253

Centisome position: 90.93

GC content: 67.81

Gene sequence:

>1047_bases
ATGAACATCATTGACCTGCGCAGCGACACCGTTACCCGGCCGAGCCCTGCCATGCGCGCCGCCATGGCGGGAGCCGAGGT
AGGGGACGACGTCTATGGCGAAGACCCGACCGTGAACCGGCTGGAGGAACTGGGCGCCGCCACGCTCGGCACGGAAGCGG
CCCTGTTCACCGCCAGCGGTACCCAGGCCAATCTTCTGGCTCTCCTGGCCCACTGCCGGCGTGGTGACGAATACATTGCC
GGCCAGACCGCCCACTGCTATCGCTACGAAGGGGGCGGTGCCGCCGCTTTGGGGGGCATCCAGCCCCAGCCCCTGGAGGT
GGAACCCGACGGGACCCTCGATCTCTCAGCCGTGGCCGCCGCCATCAAGCCGGACGACCTCCACTTCGCCCGCACCCGGC
TCCTCTGCCTGGAAAACACCCATGCCGGGCGGGTTCTTCCCCTGGACTACCTGGCACGCGCCAGACGGCTCTGCAACGAG
CAGAGCCTTGGCCTGCACATGGACGGTGCCCGCATCTTCAATGCCGCCGTAAAGCTCGGCGTACCGGTCCGCGAGATCGC
CGGTCACGTGGATTCGGTGTCGGTCTGCCTCTCCAAGGGGCTCGGCGCCCCGGTGGGGTCGCTGCTCTGCGGGGGAAGGG
AGTTCGTCGCCACGGCCCGCCGGTGGCGCAAGGCGGTCGGAGGAGGCATGCGCCAGGCCGGCATCCTGGCGGCCGCCGGC
ATCCTGGCGCTGACCGAAAACGTTGACCGGCTCGCCGAGGACCATGCCAACGCCCGCCGCTTGGCCGAGGGGTTGGCCGC
CATCCCGGGGCTGGGCCTTGACCCGTCCCAGGTCCAGACCAACATGGTATTCCTGTGCCTCCCTCTCCGGACGGCGGACC
GGCTCGCCTCATTTCTCAACGAACAGGGAATTCTCATCTCCGGCCGGGAAAGTATCCGTCTCGTCACCCACCTTGACGTA
ACCTCTTCGGACGTGGAACGGGTTATCGCGGCATTCGGCGCATTCTTCGCCGGCCACGGCGAGATGCAACCGGGAGCATC
GTCATGA

Upstream 100 bases:

>100_bases
GGTAGGCCGGCGGATATTCTCCGCGAGCCCGGCGCGTGAACTCCCGCCCACGGGCAAATGACCCGACGCACGCACACTAT
CCCCATCCCACGAGGATCCC

Downstream 100 bases:

>100_bases
TGGTCCGCAGTCTCACAGCCATGCTTGTCCTTGCCGCTACCGTGGCGCTCACACCGGCACTGCTCCATTCCGCCCCGGAC
AAGCCCGGCCGGACAGCCGA

Product: L-allo-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNIIDLRSDTVTRPSPAMRAAMAGAEVGDDVYGEDPTVNRLEELGAATLGTEAALFTASGTQANLLALLAHCRRGDEYIA
GQTAHCYRYEGGGAAALGGIQPQPLEVEPDGTLDLSAVAAAIKPDDLHFARTRLLCLENTHAGRVLPLDYLARARRLCNE
QSLGLHMDGARIFNAAVKLGVPVREIAGHVDSVSVCLSKGLGAPVGSLLCGGREFVATARRWRKAVGGGMRQAGILAAAG
ILALTENVDRLAEDHANARRLAEGLAAIPGLGLDPSQVQTNMVFLCLPLRTADRLASFLNEQGILISGRESIRLVTHLDV
TSSDVERVIAAFGAFFAGHGEMQPGASS

Sequences:

>Translated_348_residues
MNIIDLRSDTVTRPSPAMRAAMAGAEVGDDVYGEDPTVNRLEELGAATLGTEAALFTASGTQANLLALLAHCRRGDEYIA
GQTAHCYRYEGGGAAALGGIQPQPLEVEPDGTLDLSAVAAAIKPDDLHFARTRLLCLENTHAGRVLPLDYLARARRLCNE
QSLGLHMDGARIFNAAVKLGVPVREIAGHVDSVSVCLSKGLGAPVGSLLCGGREFVATARRWRKAVGGGMRQAGILAAAG
ILALTENVDRLAEDHANARRLAEGLAAIPGLGLDPSQVQTNMVFLCLPLRTADRLASFLNEQGILISGRESIRLVTHLDV
TSSDVERVIAAFGAFFAGHGEMQPGASS
>Mature_348_residues
MNIIDLRSDTVTRPSPAMRAAMAGAEVGDDVYGEDPTVNRLEELGAATLGTEAALFTASGTQANLLALLAHCRRGDEYIA
GQTAHCYRYEGGGAAALGGIQPQPLEVEPDGTLDLSAVAAAIKPDDLHFARTRLLCLENTHAGRVLPLDYLARARRLCNE
QSLGLHMDGARIFNAAVKLGVPVREIAGHVDSVSVCLSKGLGAPVGSLLCGGREFVATARRWRKAVGGGMRQAGILAAAG
ILALTENVDRLAEDHANARRLAEGLAAIPGLGLDPSQVQTNMVFLCLPLRTADRLASFLNEQGILISGRESIRLVTHLDV
TSSDVERVIAAFGAFFAGHGEMQPGASS

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=335, Percent_Identity=56.4179104477612, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI193206524, Length=344, Percent_Identity=44.4767441860465, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI193206522, Length=344, Percent_Identity=44.4767441860465, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI193206520, Length=344, Percent_Identity=44.4767441860465, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6320789, Length=341, Percent_Identity=39.0029325513196, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI21355439, Length=314, Percent_Identity=47.4522292993631, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36563; Mature: 36563

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDLRSDTVTRPSPAMRAAMAGAEVGDDVYGEDPTVNRLEELGAATLGTEAALFTASG
CCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHCCCCEEEEEECC
TQANLLALLAHCRRGDEYIAGQTAHCYRYEGGGAAALGGIQPQPLEVEPDGTLDLSAVAA
CHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCCCEEECCCCCEEHHHHHH
AIKPDDLHFARTRLLCLENTHAGRVLPLDYLARARRLCNEQSLGLHMDGARIFNAAVKLG
HCCCCCHHHHHHHEEEEECCCCCCEECHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCC
VPVREIAGHVDSVSVCLSKGLGAPVGSLLCGGREFVATARRWRKAVGGGMRQAGILAAAG
CCHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ILALTENVDRLAEDHANARRLAEGLAAIPGLGLDPSQVQTNMVFLCLPLRTADRLASFLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEEEECCCHHHHHHHHHC
EQGILISGRESIRLVTHLDVTSSDVERVIAAFGAFFAGHGEMQPGASS
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNIIDLRSDTVTRPSPAMRAAMAGAEVGDDVYGEDPTVNRLEELGAATLGTEAALFTASG
CCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHCCCCEEEEEECC
TQANLLALLAHCRRGDEYIAGQTAHCYRYEGGGAAALGGIQPQPLEVEPDGTLDLSAVAA
CHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCCCEEECCCCCEEHHHHHH
AIKPDDLHFARTRLLCLENTHAGRVLPLDYLARARRLCNEQSLGLHMDGARIFNAAVKLG
HCCCCCHHHHHHHEEEEECCCCCCEECHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCC
VPVREIAGHVDSVSVCLSKGLGAPVGSLLCGGREFVATARRWRKAVGGGMRQAGILAAAG
CCHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ILALTENVDRLAEDHANARRLAEGLAAIPGLGLDPSQVQTNMVFLCLPLRTADRLASFLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEEEECCCHHHHHHHHHC
EQGILISGRESIRLVTHLDVTSSDVERVIAAFGAFFAGHGEMQPGASS
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]