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The map label for this gene is hemK [H]

Identifier: 39998193

GI number: 39998193

Start: 3406891

End: 3407745

Strand: Reverse

Name: hemK [H]

Synonym: GSU3103

Alternate gene names: 39998193

Gene position: 3407745-3406891 (Counterclockwise)

Preceding gene: 39998194

Following gene: 39998192

Centisome position: 89.35

GC content: 63.63

Gene sequence:

>855_bases
ATGACGGAAAAGCCGGAAATCTGGACCATTCGCAAGGTTCTCGACTGGACCAGGGGCTACCTGGCTGAAAAGGGAGTCGA
GAATGCTCGCCTGGAAACCGAATGGCTCCTGAGCGCCGCCCTGGGGCTTGATCGGGTGGGGCTCTACGTCAATTTCGACA
AACCCCTGAACCCGGAGGAACTGGCCGCCTGCCGCGGACTCGTGGCGCGCCGGGCCAAGCGGGAACCACTTCAGTATATT
CTCGGTACCCAGGAGTTCTGCGGTCTCGACTTCGTCGTGACGCCGTCGGTTCTCATCCCCCGGCATGACACCGAGGTGAT
TGTGGAAGAGGCGCTCCGTCGCGCCCCCCATGCTGCTGCGGTCCTCGATATCGGTGTGGGCAGCGGCTGTATCGCCGTGG
CCTTGGCCAAGCAACTTCCCCACGCCCAGGTAGTGGGAGTGGAGCAGTCTCCCGGCGCCATCGCCCTGGCCCAGCGGAAC
GCGGAACGGCACGGCGCGCGGGTGACCCTCTTCGAGGGTTCCCTGTTCGAACCCTTAGGAGACCAGCGGTTCGACCTGAT
CGTCTCCAATCCCCCTTATATCCCCACCGCCGACCTGGAGGCGCTTCAGCCGGAGGTTCGGGAGTACGAGCCCCGTGCCG
CCCTGGACGGTGGATCCGACGGTCTCGATTTCTATCGGCTTATCGTTCCGGCCGCTCCGGAGTACCTGAATCCCGGCGGC
TGGCTCATGGTCGAACTGGGCATCGGTCAGGCGGAGACGGTGCTCGGCATGTTCTCCCGTACCGGTTTCTGCGATTGTTT
TACCGCTCAAGACCCCAACGGCATAGACCGGGTGGTCGGCGGAAGGATCGGATAG

Upstream 100 bases:

>100_bases
ATCGCCGAGGTGGTGGATGCGCTCCGCACTCATTATCAGATGGAGGCCCTGAAGGCCCAGGCCGAAGCGGCCTGATCCGC
GCGGACAGCGGAGGCGGCAG

Downstream 100 bases:

>100_bases
GCCGGGTTTCACGCGAGACTTGGCCCCGAACACACTATCACGACAGAGGCATTCCATTGGACAAGCTGATCATCAAAGGT
GGAAAGAAACTGATCGGAGA

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTEKPEIWTIRKVLDWTRGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNPEELAACRGLVARRAKREPLQYI
LGTQEFCGLDFVVTPSVLIPRHDTEVIVEEALRRAPHAAAVLDIGVGSGCIAVALAKQLPHAQVVGVEQSPGAIALAQRN
AERHGARVTLFEGSLFEPLGDQRFDLIVSNPPYIPTADLEALQPEVREYEPRAALDGGSDGLDFYRLIVPAAPEYLNPGG
WLMVELGIGQAETVLGMFSRTGFCDCFTAQDPNGIDRVVGGRIG

Sequences:

>Translated_284_residues
MTEKPEIWTIRKVLDWTRGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNPEELAACRGLVARRAKREPLQYI
LGTQEFCGLDFVVTPSVLIPRHDTEVIVEEALRRAPHAAAVLDIGVGSGCIAVALAKQLPHAQVVGVEQSPGAIALAQRN
AERHGARVTLFEGSLFEPLGDQRFDLIVSNPPYIPTADLEALQPEVREYEPRAALDGGSDGLDFYRLIVPAAPEYLNPGG
WLMVELGIGQAETVLGMFSRTGFCDCFTAQDPNGIDRVVGGRIG
>Mature_283_residues
TEKPEIWTIRKVLDWTRGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNPEELAACRGLVARRAKREPLQYIL
GTQEFCGLDFVVTPSVLIPRHDTEVIVEEALRRAPHAAAVLDIGVGSGCIAVALAKQLPHAQVVGVEQSPGAIALAQRNA
ERHGARVTLFEGSLFEPLGDQRFDLIVSNPPYIPTADLEALQPEVREYEPRAALDGGSDGLDFYRLIVPAAPEYLNPGGW
LMVELGIGQAETVLGMFSRTGFCDCFTAQDPNGIDRVVGGRIG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=268, Percent_Identity=35.4477611940299, Blast_Score=135, Evalue=4e-32,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=35.1851851851852, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI87082085, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324266, Length=208, Percent_Identity=29.3269230769231, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24582226, Length=239, Percent_Identity=36.4016736401674, Blast_Score=137, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30972; Mature: 30841

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKPEIWTIRKVLDWTRGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNPEE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
LAACRGLVARRAKREPLQYILGTQEFCGLDFVVTPSVLIPRHDTEVIVEEALRRAPHAAA
HHHHHHHHHHHHHCCHHHHHHCCHHHHCCEEEECCCEEECCCCHHHHHHHHHHHCCCCEE
VLDIGVGSGCIAVALAKQLPHAQVVGVEQSPGAIALAQRNAERHGARVTLFEGSLFEPLG
EEEECCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHCCCEEEEECCCCCCCCC
DQRFDLIVSNPPYIPTADLEALQPEVREYEPRAALDGGSDGLDFYRLIVPAAPEYLNPGG
CCCEEEEECCCCCCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
WLMVELGIGQAETVLGMFSRTGFCDCFTAQDPNGIDRVVGGRIG
EEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCC
>Mature Secondary Structure 
TEKPEIWTIRKVLDWTRGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNPEE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
LAACRGLVARRAKREPLQYILGTQEFCGLDFVVTPSVLIPRHDTEVIVEEALRRAPHAAA
HHHHHHHHHHHHHCCHHHHHHCCHHHHCCEEEECCCEEECCCCHHHHHHHHHHHCCCCEE
VLDIGVGSGCIAVALAKQLPHAQVVGVEQSPGAIALAQRNAERHGARVTLFEGSLFEPLG
EEEECCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCHHHCCCEEEEECCCCCCCCC
DQRFDLIVSNPPYIPTADLEALQPEVREYEPRAALDGGSDGLDFYRLIVPAAPEYLNPGG
CCCEEEEECCCCCCCCCCHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
WLMVELGIGQAETVLGMFSRTGFCDCFTAQDPNGIDRVVGGRIG
EEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]