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The map label for this gene is hisH

Identifier: 39998187

GI number: 39998187

Start: 3401273

End: 3401902

Strand: Reverse

Name: hisH

Synonym: GSU3097

Alternate gene names: 39998187

Gene position: 3401902-3401273 (Counterclockwise)

Preceding gene: 39998188

Following gene: 39998186

Centisome position: 89.19

GC content: 61.11

Gene sequence:

>630_bases
ATGACCAAGATTGCAATTATCGACTACGGCATGGGGAATCTCCGGTCGGTCCAGAAGGGGTTCGAGAAGGTGGGCTTCGA
GGCGGTTGTGACTGCCGACCCCAAGGTGGTGCTGGAGGCGGAAAAGATCGTCCTTCCCGGCGTGGGAGCCTTTCGCGACT
GCATGCGCAACCTGGAGCAGGGGGGGTTCGTGGAGCCGATCCTCAGGGTGATCCGGGAGGGGCGCCCCTTTCTCGGCATC
TGCGTCGGGATGCAGCTCCTCTTGACCGACAGCGTGGAGTTCGGCCTCTACCAGGGGCTGAACGTCATTCCTGGCCACGT
CCTCCGTTTTCCCGAGGGGATGCGCGAAGGAGGCGAGGAACTCAAAGTCCCCCACATGGGGTGGAACCAGCTCTCCATCA
AACGCCGCCCACCGGCCTTTGCCGAGGTGGAGGACGGGGCCAACGTCTATTTCGTCCATTCATACTATGAAATGCCCGAC
GACGAGAGTGTCATCGCCGCCACCTGCACCTACGGCGTCGAGTTCTGCGCCGCCATCTGGAAGGACAACATCGTCGCCAC
CCAGTTCCATCCCGAGAAGTCACAGGCGGTGGGGCTGTCCATACTCAAGAACTTCGGAGAGATGAAGTGA

Upstream 100 bases:

>100_bases
GTCGAGGCGTGCTTCAAGGCGTTTGCCCGGGCCGTGGACCAGGCAACCCAGGTGGACTCCCGGATTCAGGGGGTCATGTC
GACGAAGGGCAAGCTCTGAT

Downstream 100 bases:

>100_bases
TCGTCATACCTGCCATTGACCTGAAAGAAGGAAAGTGCGTCCGCCTCGAACAGGGGCTCATGGAAAAGGATACCGTTTTC
TGTGACAATCCCGCCGACCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTKIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKIVLPGVGAFRDCMRNLEQGGFVEPILRVIREGRPFLGI
CVGMQLLLTDSVEFGLYQGLNVIPGHVLRFPEGMREGGEELKVPHMGWNQLSIKRRPPAFAEVEDGANVYFVHSYYEMPD
DESVIAATCTYGVEFCAAIWKDNIVATQFHPEKSQAVGLSILKNFGEMK

Sequences:

>Translated_209_residues
MTKIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKIVLPGVGAFRDCMRNLEQGGFVEPILRVIREGRPFLGI
CVGMQLLLTDSVEFGLYQGLNVIPGHVLRFPEGMREGGEELKVPHMGWNQLSIKRRPPAFAEVEDGANVYFVHSYYEMPD
DESVIAATCTYGVEFCAAIWKDNIVATQFHPEKSQAVGLSILKNFGEMK
>Mature_208_residues
TKIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKIVLPGVGAFRDCMRNLEQGGFVEPILRVIREGRPFLGIC
VGMQLLLTDSVEFGLYQGLNVIPGHVLRFPEGMREGGEELKVPHMGWNQLSIKRRPPAFAEVEDGANVYFVHSYYEMPDD
ESVIAATCTYGVEFCAAIWKDNIVATQFHPEKSQAVGLSILKNFGEMK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=206, Percent_Identity=41.747572815534, Blast_Score=135, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=36.5740740740741, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_GEOSL (P60599)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954138.1
- ProteinModelPortal:   P60599
- SMR:   P60599
- GeneID:   2687589
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3097
- NMPDR:   fig|243231.1.peg.3076
- TIGR:   GSU3097
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   GSUL243231:GSU_3097-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23185; Mature: 23054

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 190-190 ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKIVLPGVGAFRDCMRNLEQ
CCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECEEEECCCHHHHHHHHHHHC
GGFVEPILRVIREGRPFLGICVGMQLLLTDSVEFGLYQGLNVIPGHVLRFPEGMREGGEE
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHCCHHHHCCCCC
LKVPHMGWNQLSIKRRPPAFAEVEDGANVYFVHSYYEMPDDESVIAATCTYGVEFCAAIW
CCCCCCCCCCEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHH
KDNIVATQFHPEKSQAVGLSILKNFGEMK
CCCEEEEEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKIAIIDYGMGNLRSVQKGFEKVGFEAVVTADPKVVLEAEKIVLPGVGAFRDCMRNLEQ
CEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECEEEECCCHHHHHHHHHHHC
GGFVEPILRVIREGRPFLGICVGMQLLLTDSVEFGLYQGLNVIPGHVLRFPEGMREGGEE
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHCCHHHHCCCCC
LKVPHMGWNQLSIKRRPPAFAEVEDGANVYFVHSYYEMPDDESVIAATCTYGVEFCAAIW
CCCCCCCCCCEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHH
KDNIVATQFHPEKSQAVGLSILKNFGEMK
CCCEEEEEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA