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The map label for this gene is pilB [H]

Identifier: 39997703

GI number: 39997703

Start: 2877043

End: 2878980

Strand: Reverse

Name: pilB [H]

Synonym: GSU2609

Alternate gene names: 39997703

Gene position: 2878980-2877043 (Counterclockwise)

Preceding gene: 39997704

Following gene: 39997702

Centisome position: 75.48

GC content: 63.73

Gene sequence:

>1938_bases
ATGTCCCAGAAAAAGGTTGGCGAGATACTTATCGAGCACCGGCTGATCTCCGAGGATCAGCTCCGCGAGGCCCTGGAACT
GCAGAAGGTCTTCCCCGACCAACCCGTGGGGCAACTGCTCTGCAAGCTCGGGTTCCTGAGCGAGAGTGAGCTGTCCTATA
TCCTGGAGCAGACCGGCAAGCGCCAGAAGCTGGGCGATATCCTCATCAGGGAGCGCCTGGTCGACGAGGAGCGCCTGAAC
CAGGCCCGGGTCGCCGCCAAGCGGGACGGCTCCACGCTGGAGCGTGCCCTCCGCAAGCTGCGTCTCGTCGAAGAGGAGCC
TCTGGCCAAGACCATTGCCACCCAGTACGACCTCTCCTTTGTTCACATCAACACCCTGGAGATCGAGCCGGACCTCGCCC
GCTGCATCAACCCCAACTATGCCCAGCGCCAGCGGATCGTCCCCATCTCCCGCATCGGCAACACCATCACTCTGGCCATG
GCCTATCCCATCAAGCTTCATGAGCTGAAGGAGCTTGAGCAGAGCATCAAGTCGCGGATCATCCCGGTCATTGCCATGGA
GAGCGAGATCATCCAGGCCCAGCAGCGCCTCTACAAGACCGCCGCCAGCGCCGCCCACGCCCTTACCCTCGATGAGGCCG
ACCTGGAGATCGCGCCGGGAAGCATCGTCGACATCCTGAGCTCCGGCGCCGGGGAGGATGAGCCGGACATCGATGACGAG
GTGCGCACGATCACCGAGCGCGACAGTGTCATCGTCAAGCTCGTCAACAAGATCATCTTCGACGCCCACCAGAACCGCGC
CTCGGATATCCATATCGAGCCCTATCCCGGCAAGAACGACGTGATCGTCAGGATGCGGGTCGACGGCAGCTGCAAGGTGT
ACCAGCGCATCCCGTTCAAGTACAAGTACGCCATCCCCTCGCGCCTCAAGATCATGGCCGAGCTGGACATCGCCGAGAAG
CGCAAGCCCCAGGACGGCAAGATCAATTTCAAGAAATTCGGCCCCCTGGACCTGGAACTGCGGATCGCCACCATGCCAAC
GGCCGGTGGACTCGAGGACGTGGTCATCCGGCTCCTGAACACGGGCCAGGCCTATTCATTCGACAGTCTCAGCCTCACCG
ACCGCAATATGCGCATCTTCGGGGAATCCATCACGAAGCCCTACGGCCTTGTCCTGGTGGTGGGCCCCACCGGAAGCGGC
AAGACCACCACCCTCCACGCGGCCATCGCCCGCATCAACCGACCCGAGGTGAAGATCTGGACCGCCGAGGACCCGGTGGA
GATCACCCAGAAGGGGCTCCGGCAGGTTCAGGTCAACCAGCGCATCGGCCTCACCTTTGCCGCGGCACTGCGCTCGTTCC
TGCGGCTCGACCCGGACGTGATCATGGTGGGCGAGATGCGGGACGAAGAGACCGCCTCCATCGCGGTGGAGGCGTCCCTC
ACCGGGCACCTGGTCCTGTCGACCCTGCACACCAACTCGGCCCCGGAGACGGTCACCCGCCTCCTGGAAATGGGACTCGA
CCCCTTCAGCTTCTCCGATTCGCTTCTCTGCGTCGTGGCCCAGCGCCTGGCCCGCCGCCTCTGCGAGGATTGCCGCGAGC
TTTACCGCCCTGACCGCAAGGAGCTCTCCGAGATCATCGAGGAGTACGGCGAGGAGCAGTTCGCCGCCACGGGACTGCTG
GGCAACGAGGTGGTCCTGGCCCGGCCGGTGGGGTGCACCACCTGCAACCAGAGCGGCTACCGGGGGCGCCTCGGCATTCA
CGAGGTGCTCGAAGGCACCGACACCATGAAGAGCCTCGTCAAGAAGAAGTCCGACACCGAGATCATCCGGCGGCAGGCCA
TGGCCGACGGCATGACCACCCTGCGGCAGGACGGCATCCTCAAGGTCTTCCAGGGGCTCACCGACATCCACGAAGTACGC
AAGGTCTGCCTCAAGTAA

Upstream 100 bases:

>100_bases
AATGCGGCATTGAACCCCCTGTTCCGCGATCTGCTCGATTTCAACGGCTACTCGATCGTTTCCTACTGAACGGCGGCCGA
TCCCGAGGTTCTTTGCCCCC

Downstream 100 bases:

>100_bases
CGATGGTGTAAACCATAAAATGTTTGCATTTTAGCCCCCTTTGCTATACAGTACCTCCCTCCGCGGAACTGCCGCACCAT
ATTTCTCCCCCCAGGTGACC

Product: type IV pilus assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSQKKVGEILIEHRLISEDQLREALELQKVFPDQPVGQLLCKLGFLSESELSYILEQTGKRQKLGDILIRERLVDEERLN
QARVAAKRDGSTLERALRKLRLVEEEPLAKTIATQYDLSFVHINTLEIEPDLARCINPNYAQRQRIVPISRIGNTITLAM
AYPIKLHELKELEQSIKSRIIPVIAMESEIIQAQQRLYKTAASAAHALTLDEADLEIAPGSIVDILSSGAGEDEPDIDDE
VRTITERDSVIVKLVNKIIFDAHQNRASDIHIEPYPGKNDVIVRMRVDGSCKVYQRIPFKYKYAIPSRLKIMAELDIAEK
RKPQDGKINFKKFGPLDLELRIATMPTAGGLEDVVIRLLNTGQAYSFDSLSLTDRNMRIFGESITKPYGLVLVVGPTGSG
KTTTLHAAIARINRPEVKIWTAEDPVEITQKGLRQVQVNQRIGLTFAAALRSFLRLDPDVIMVGEMRDEETASIAVEASL
TGHLVLSTLHTNSAPETVTRLLEMGLDPFSFSDSLLCVVAQRLARRLCEDCRELYRPDRKELSEIIEEYGEEQFAATGLL
GNEVVLARPVGCTTCNQSGYRGRLGIHEVLEGTDTMKSLVKKKSDTEIIRRQAMADGMTTLRQDGILKVFQGLTDIHEVR
KVCLK

Sequences:

>Translated_645_residues
MSQKKVGEILIEHRLISEDQLREALELQKVFPDQPVGQLLCKLGFLSESELSYILEQTGKRQKLGDILIRERLVDEERLN
QARVAAKRDGSTLERALRKLRLVEEEPLAKTIATQYDLSFVHINTLEIEPDLARCINPNYAQRQRIVPISRIGNTITLAM
AYPIKLHELKELEQSIKSRIIPVIAMESEIIQAQQRLYKTAASAAHALTLDEADLEIAPGSIVDILSSGAGEDEPDIDDE
VRTITERDSVIVKLVNKIIFDAHQNRASDIHIEPYPGKNDVIVRMRVDGSCKVYQRIPFKYKYAIPSRLKIMAELDIAEK
RKPQDGKINFKKFGPLDLELRIATMPTAGGLEDVVIRLLNTGQAYSFDSLSLTDRNMRIFGESITKPYGLVLVVGPTGSG
KTTTLHAAIARINRPEVKIWTAEDPVEITQKGLRQVQVNQRIGLTFAAALRSFLRLDPDVIMVGEMRDEETASIAVEASL
TGHLVLSTLHTNSAPETVTRLLEMGLDPFSFSDSLLCVVAQRLARRLCEDCRELYRPDRKELSEIIEEYGEEQFAATGLL
GNEVVLARPVGCTTCNQSGYRGRLGIHEVLEGTDTMKSLVKKKSDTEIIRRQAMADGMTTLRQDGILKVFQGLTDIHEVR
KVCLK
>Mature_644_residues
SQKKVGEILIEHRLISEDQLREALELQKVFPDQPVGQLLCKLGFLSESELSYILEQTGKRQKLGDILIRERLVDEERLNQ
ARVAAKRDGSTLERALRKLRLVEEEPLAKTIATQYDLSFVHINTLEIEPDLARCINPNYAQRQRIVPISRIGNTITLAMA
YPIKLHELKELEQSIKSRIIPVIAMESEIIQAQQRLYKTAASAAHALTLDEADLEIAPGSIVDILSSGAGEDEPDIDDEV
RTITERDSVIVKLVNKIIFDAHQNRASDIHIEPYPGKNDVIVRMRVDGSCKVYQRIPFKYKYAIPSRLKIMAELDIAEKR
KPQDGKINFKKFGPLDLELRIATMPTAGGLEDVVIRLLNTGQAYSFDSLSLTDRNMRIFGESITKPYGLVLVVGPTGSGK
TTTLHAAIARINRPEVKIWTAEDPVEITQKGLRQVQVNQRIGLTFAAALRSFLRLDPDVIMVGEMRDEETASIAVEASLT
GHLVLSTLHTNSAPETVTRLLEMGLDPFSFSDSLLCVVAQRLARRLCEDCRELYRPDRKELSEIIEEYGEEQFAATGLLG
NEVVLARPVGCTTCNQSGYRGRLGIHEVLEGTDTMKSLVKKKSDTEIIRRQAMADGMTTLRQDGILKVFQGLTDIHEVRK
VCLK

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=428, Percent_Identity=41.588785046729, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1786296, Length=400, Percent_Identity=41, Blast_Score=285, Evalue=6e-78,
Organism=Escherichia coli, GI87082188, Length=155, Percent_Identity=43.8709677419355, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 72309; Mature: 72177

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKKVGEILIEHRLISEDQLREALELQKVFPDQPVGQLLCKLGFLSESELSYILEQTGK
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
RQKLGDILIRERLVDEERLNQARVAAKRDGSTLERALRKLRLVEEEPLAKTIATQYDLSF
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCEEE
VHINTLEIEPDLARCINPNYAQRQRIVPISRIGNTITLAMAYPIKLHELKELEQSIKSRI
EEEEEEEECHHHHHHCCCCHHHHCCCCCHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCC
IPVIAMESEIIQAQQRLYKTAASAAHALTLDEADLEIAPGSIVDILSSGAGEDEPDIDDE
CEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHCCCCCCCCCCCHH
VRTITERDSVIVKLVNKIIFDAHQNRASDIHIEPYPGKNDVIVRMRVDGSCKVYQRIPFK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEEECCCCEEEECCCEE
YKYAIPSRLKIMAELDIAEKRKPQDGKINFKKFGPLDLELRIATMPTAGGLEDVVIRLLN
EEECCCHHHHHHHHCCHHHHCCCCCCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHC
TGQAYSFDSLSLTDRNMRIFGESITKPYGLVLVVGPTGSGKTTTLHAAIARINRPEVKIW
CCCCCCCCCCEECCCCHHEECHHCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEE
TAEDPVEITQKGLRQVQVNQRIGLTFAAALRSFLRLDPDVIMVGEMRDEETASIAVEASL
ECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECC
TGHLVLSTLHTNSAPETVTRLLEMGLDPFSFSDSLLCVVAQRLARRLCEDCRELYRPDRK
CCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELSEIIEEYGEEQFAATGLLGNEVVLARPVGCTTCNQSGYRGRLGIHEVLEGTDTMKSLV
HHHHHHHHHCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHH
KKKSDTEIIRRQAMADGMTTLRQDGILKVFQGLTDIHEVRKVCLK
HCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQKKVGEILIEHRLISEDQLREALELQKVFPDQPVGQLLCKLGFLSESELSYILEQTGK
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
RQKLGDILIRERLVDEERLNQARVAAKRDGSTLERALRKLRLVEEEPLAKTIATQYDLSF
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCEEE
VHINTLEIEPDLARCINPNYAQRQRIVPISRIGNTITLAMAYPIKLHELKELEQSIKSRI
EEEEEEEECHHHHHHCCCCHHHHCCCCCHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCC
IPVIAMESEIIQAQQRLYKTAASAAHALTLDEADLEIAPGSIVDILSSGAGEDEPDIDDE
CEEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHCCCCCCCCCCCHH
VRTITERDSVIVKLVNKIIFDAHQNRASDIHIEPYPGKNDVIVRMRVDGSCKVYQRIPFK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEEECCCCEEEECCCEE
YKYAIPSRLKIMAELDIAEKRKPQDGKINFKKFGPLDLELRIATMPTAGGLEDVVIRLLN
EEECCCHHHHHHHHCCHHHHCCCCCCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHC
TGQAYSFDSLSLTDRNMRIFGESITKPYGLVLVVGPTGSGKTTTLHAAIARINRPEVKIW
CCCCCCCCCCEECCCCHHEECHHCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEE
TAEDPVEITQKGLRQVQVNQRIGLTFAAALRSFLRLDPDVIMVGEMRDEETASIAVEASL
ECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECC
TGHLVLSTLHTNSAPETVTRLLEMGLDPFSFSDSLLCVVAQRLARRLCEDCRELYRPDRK
CCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ELSEIIEEYGEEQFAATGLLGNEVVLARPVGCTTCNQSGYRGRLGIHEVLEGTDTMKSLV
HHHHHHHHHCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHH
KKKSDTEIIRRQAMADGMTTLRQDGILKVFQGLTDIHEVRKVCLK
HCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]