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The map label for this gene is icaB [H]

Identifier: 39997638

GI number: 39997638

Start: 2806022

End: 2806798

Strand: Reverse

Name: icaB [H]

Synonym: GSU2543

Alternate gene names: 39997638

Gene position: 2806798-2806022 (Counterclockwise)

Preceding gene: 39997639

Following gene: 39997637

Centisome position: 73.59

GC content: 61.52

Gene sequence:

>777_bases
ATGTTCCTGGCGTCCTTCTCGTCCGCCGCCCGGGCCGTGCCCCGGCCGGTTTCGGCCGCGGATTTCTCGGTTCCCATCCT
CCTTTACCACCGCTTCGGCCCCACCGTTGCCGACGGCATGACCATCAAAACATCGGTCTTCGAGGAACACCTGAAGTATC
TGCGGGACAACGGCTACCGGGTGATTCCCCTGCGGCAGGTGGTGGACTTCTATCTGAAAAAGGGACCGGCGCCGCCCCCC
AAGTCGGTGGTGATCGTGGAGGACGACGCCCATAAGTCGGTCTATACCGACATGCTGCCGTTGGTGAAGAAGTACCGGGT
GCCGGTGACGGTCTTCATCTACCCGTCGGCCGTCTCCAACGCGAAGTATGCCATGACTTGGGACCAGCTCCGGGAGCTGA
AAAAAACGGGGCTCTTCGATTTCCAGTCCCACACCTACTGGCATCCCAACTTCAAGAAGGAGCGGAAGAAACTCCCCCCG
GCGGAGTTTGACCGGCTGGTGGAGTCGCAGCTGAAAAAATCGAGGGAAAAGATCGAGAAGGAACTCGGGGTGACGGTGGA
CATGCTCGCCTGGCCCTTCGGCATTTATGACGACGACCTGATCCGCCGGGCCGGGACGCTGGGGTACCGGGCCACCTTCA
CCATCGACCGTCGCCACGTGACGCCGGCCGAGAGCGTCATGAAGCTGCCCCGTTATCTCATGATCAACGCCGACCAGGGG
AAGGTCTTCGCCCAGATCCTGGCCGGGAGCGGACCCCGGAGAAATGTCGTCTACTGA

Upstream 100 bases:

>100_bases
CCGTCACTACCCCTGTGGCGGTCTTCGGGCCGTAATTCCCGTAGTTATTTCCTTAGTGGAGGTTGTAGCGCAAATGCTGT
TCCGCCTGATTGTCGCGGTT

Downstream 100 bases:

>100_bases
TCCGCCCAAAAAGCATCCCTGCCCCGACTGCCGGTTCTGCCAGTGGTGCGGCGATGACCGCTGTTCCCTCTGCCTGCGGG
GGTGCAAGAAAGGCAAAAAG

Product: polysaccharide deacetylase domain-containing protein

Products: NA

Alternate protein names: PNAG N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase; Biofilm polysaccharide intercellular adhesin deacetylase; Biofilm PIA deacetylase; Intercellular adhesion protein B [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MFLASFSSAARAVPRPVSAADFSVPILLYHRFGPTVADGMTIKTSVFEEHLKYLRDNGYRVIPLRQVVDFYLKKGPAPPP
KSVVIVEDDAHKSVYTDMLPLVKKYRVPVTVFIYPSAVSNAKYAMTWDQLRELKKTGLFDFQSHTYWHPNFKKERKKLPP
AEFDRLVESQLKKSREKIEKELGVTVDMLAWPFGIYDDDLIRRAGTLGYRATFTIDRRHVTPAESVMKLPRYLMINADQG
KVFAQILAGSGPRRNVVY

Sequences:

>Translated_258_residues
MFLASFSSAARAVPRPVSAADFSVPILLYHRFGPTVADGMTIKTSVFEEHLKYLRDNGYRVIPLRQVVDFYLKKGPAPPP
KSVVIVEDDAHKSVYTDMLPLVKKYRVPVTVFIYPSAVSNAKYAMTWDQLRELKKTGLFDFQSHTYWHPNFKKERKKLPP
AEFDRLVESQLKKSREKIEKELGVTVDMLAWPFGIYDDDLIRRAGTLGYRATFTIDRRHVTPAESVMKLPRYLMINADQG
KVFAQILAGSGPRRNVVY
>Mature_258_residues
MFLASFSSAARAVPRPVSAADFSVPILLYHRFGPTVADGMTIKTSVFEEHLKYLRDNGYRVIPLRQVVDFYLKKGPAPPP
KSVVIVEDDAHKSVYTDMLPLVKKYRVPVTVFIYPSAVSNAKYAMTWDQLRELKKTGLFDFQSHTYWHPNFKKERKKLPP
AEFDRLVESQLKKSREKIEKELGVTVDMLAWPFGIYDDDLIRRAGTLGYRATFTIDRRHVTPAESVMKLPRYLMINADQG
KVFAQILAGSGPRRNVVY

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. N-deacetylation is crucial for attachment of the polysaccharide to the bacterial cell surface; it leads to the introd

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Secreted, cell wall. Note=Attached to the cell surface [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=253, Percent_Identity=24.5059288537549, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786322, Length=248, Percent_Identity=24.1935483870968, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 29545; Mature: 29545

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLASFSSAARAVPRPVSAADFSVPILLYHRFGPTVADGMTIKTSVFEEHLKYLRDNGYR
CEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCHHCCEEEHHHHHHHHHHHHHHCCEE
VIPLRQVVDFYLKKGPAPPPKSVVIVEDDAHKSVYTDMLPLVKKYRVPVTVFIYPSAVSN
EEEHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AKYAMTWDQLRELKKTGLFDFQSHTYWHPNFKKERKKLPPAEFDRLVESQLKKSREKIEK
CEEEECHHHHHHHHHCCCCCCCCCCEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELGVTVDMLAWPFGIYDDDLIRRAGTLGYRATFTIDRRHVTPAESVMKLPRYLMINADQG
HHCCEEEHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCC
KVFAQILAGSGPRRNVVY
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFLASFSSAARAVPRPVSAADFSVPILLYHRFGPTVADGMTIKTSVFEEHLKYLRDNGYR
CEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCHHCCEEEHHHHHHHHHHHHHHCCEE
VIPLRQVVDFYLKKGPAPPPKSVVIVEDDAHKSVYTDMLPLVKKYRVPVTVFIYPSAVSN
EEEHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AKYAMTWDQLRELKKTGLFDFQSHTYWHPNFKKERKKLPPAEFDRLVESQLKKSREKIEK
CEEEECHHHHHHHHHCCCCCCCCCCEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELGVTVDMLAWPFGIYDDDLIRRAGTLGYRATFTIDRRHVTPAESVMKLPRYLMINADQG
HHCCEEEHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCC
KVFAQILAGSGPRRNVVY
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA