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The map label for this gene is puuR [H]

Identifier: 39997635

GI number: 39997635

Start: 2804108

End: 2804653

Strand: Reverse

Name: puuR [H]

Synonym: GSU2540

Alternate gene names: 39997635

Gene position: 2804653-2804108 (Counterclockwise)

Preceding gene: 39997637

Following gene: 39997634

Centisome position: 73.53

GC content: 60.62

Gene sequence:

>546_bases
GTGAAGATCGGCGAGCGGTTGAAGCGGCTCAGGATGATCAACTCCCTCACCCAGGAGGAATTGGCCAATCGGGCCGACCT
GACCAAGGGATACATCTCCCAGCTTGAGAACGACGCCACCTCTCCGTCCATCGCCACTCTGAAGGACATCCTCGACGTGT
TCGGCGTCAGCATGCAGGAGTTCTTCAGCGATCCAATCGGCGAGGACGTGGTCTACGGCAAGGATGCCCGGGTTCAGCCC
TCGGCCGACGATGCGGCCGTGATGGTGGAACTCCTCGTCCCCGGCGCCCAGAACCGGGAGATGGACCCGGCCCTGGTGAC
CCTGGCCCCGGGCGAGGAGATGGACGAGCAGAACTTCCACGAGGGAGAGGAGTTCGGCTTCGTCCTTCTGGGCCGAATCC
AGCTCCGGCTGGACGACAAGGTCTACACGCTGAAAAAGGACGAATGCTTCTACTTCACCTCGGACAAGCGCCATACGGTG
AAAAATATCGGCAAGGGGCCGGCCAAGATATTGTGGGTCGTGACCCCGCCGACATTTGATTACTGA

Upstream 100 bases:

>100_bases
AGTTTAGAAATGCTAAACCGGCTGCTTGACAGAAAGTTTAGAAATGCTAAACAGCGGGTGTAAACAAAGAGTTTAGTATT
TCTGAACCAGGAGAAACAAC

Downstream 100 bases:

>100_bases
TAACGCAATCGAAGGAGATCAACCAATGAGCAAGGTACTGATAATCGGAGCCGGCGGCGTCGGCCAGGTCGTTGCCCACA
AGTGCGCCCAGCGCAGGGAC

Product: Cro/CI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKIGERLKRLRMINSLTQEELANRADLTKGYISQLENDATSPSIATLKDILDVFGVSMQEFFSDPIGEDVVYGKDARVQP
SADDAAVMVELLVPGAQNREMDPALVTLAPGEEMDEQNFHEGEEFGFVLLGRIQLRLDDKVYTLKKDECFYFTSDKRHTV
KNIGKGPAKILWVVTPPTFDY

Sequences:

>Translated_181_residues
MKIGERLKRLRMINSLTQEELANRADLTKGYISQLENDATSPSIATLKDILDVFGVSMQEFFSDPIGEDVVYGKDARVQP
SADDAAVMVELLVPGAQNREMDPALVTLAPGEEMDEQNFHEGEEFGFVLLGRIQLRLDDKVYTLKKDECFYFTSDKRHTV
KNIGKGPAKILWVVTPPTFDY
>Mature_181_residues
MKIGERLKRLRMINSLTQEELANRADLTKGYISQLENDATSPSIATLKDILDVFGVSMQEFFSDPIGEDVVYGKDARVQP
SADDAAVMVELLVPGAQNREMDPALVTLAPGEEMDEQNFHEGEEFGFVLLGRIQLRLDDKVYTLKKDECFYFTSDKRHTV
KNIGKGPAKILWVVTPPTFDY

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=179, Percent_Identity=33.5195530726257, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20375; Mature: 20375

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGERLKRLRMINSLTQEELANRADLTKGYISQLENDATSPSIATLKDILDVFGVSMQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHH
FFSDPIGEDVVYGKDARVQPSADDAAVMVELLVPGAQNREMDPALVTLAPGEEMDEQNFH
HHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
EGEEFGFVLLGRIQLRLDDKVYTLKKDECFYFTSDKRHTVKNIGKGPAKILWVVTPPTFD
CCCCCCEEEEEEEEEEECCEEEEEECCCEEEEECCCCHHHHHCCCCCEEEEEEECCCCCC
Y
C
>Mature Secondary Structure
MKIGERLKRLRMINSLTQEELANRADLTKGYISQLENDATSPSIATLKDILDVFGVSMQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHH
FFSDPIGEDVVYGKDARVQPSADDAAVMVELLVPGAQNREMDPALVTLAPGEEMDEQNFH
HHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
EGEEFGFVLLGRIQLRLDDKVYTLKKDECFYFTSDKRHTVKNIGKGPAKILWVVTPPTFD
CCCCCCEEEEEEEEEEECCEEEEEECCCEEEEECCCCHHHHHCCCCCEEEEEEECCCCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]