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The map label for this gene is leuC1 [H]

Identifier: 39997540

GI number: 39997540

Start: 2680532

End: 2682469

Strand: Reverse

Name: leuC1 [H]

Synonym: GSU2445

Alternate gene names: 39997540

Gene position: 2682469-2680532 (Counterclockwise)

Preceding gene: 39997541

Following gene: 39997539

Centisome position: 70.33

GC content: 66.46

Gene sequence:

>1938_bases
ATGGGCAAGAACCTCGCAACCAAAATCCTCGAAGCGCACCTCGTGAAGGGCGAACTGACGCCGGGCACCGAGATCGCCCT
GAAGATCGACCATGCGCTCCTCCAGGACGCCACCGGCACCATGGCCATGCTGGAGTTCATCGCCATGGGGGTGGACCGGG
TGAAGGTGGAGCTGGCGGCCCAGTACATCGACCACAACCTGCTCCAGACCGACAACCGCAACGCCGACGACCACGTCTTC
CTCATGACCGCGGCCCAACGGTTCGGCGTCCATCTGTCCAAGCCGGGGAACGGGGTCTCCCACCAGGTCCACCTGGAGCG
CTTCGGCGTGCCGGGCCGGACCATGCTGGGAGCCGACTCCCACACCACCTCCGCCGCCGGGCTCTCCATGATCGCCATTG
GCGCCGGCGGACTCGACGTCTCCCTGGCCATGGCGGGGCACCCGTTCCACCTCCCCTGCCCGAAGATCTGGGGAGTGAAG
CTCACGGGACAGCTCCAGCCCTGGGTTTCGGCCAAGGACGTGATCCTGGAGATGCTCCGGCGCCACACCGTGAAGGGGGG
CGTGGGCAAGATCATCGAGTACTACGGCCCCGGCGTGGCGACCCTCTCGGCAACGGACCGCGCCATCATCGGCAACATGG
GGGCGGAGCTGGGAGCCACCACCTCCCTCTTCCCCTCGGACCACCGGACCCGCGAGTTCCTGGAGGCCCAGGGGCGCGGC
GAGGTATGGCAGGAGCTTGCCGCCGACCCGGACGCCACCTACGACGAGTACGAGGAAATCGATCTCGCCACGGTGGAGCC
CCTCATCGCCTGCCCCTCGTCTCCGGACAACGTGGTGCGGGTGGCCGACATCGAAGGGCTCAAGGTGGATCAGGTGCTCG
TGGGGAGTTCCGCCAACTCCGCCTTCCGGGACCTGATGACCGTCTGCCGCATCCTGGACGGGCGCCGCGTGGCCTCCCAC
GTCTCCTTCAACGTGAATCCGGGCAGCCGCCAGGTGTTGGAGAACGTGGCCGATCAAGGCGGAATCATGATGCTCCTCCT
GGCCGGGGCCCAGATCCACCAGCCCGGCTGCCTCGGCTGCATTGGCATGGGGCAGGCGCCGGGCACCGACCAGGTAAGTC
TGCGCACCTTCCCCCGCAACTTTCCCGGCCGCAGCGGAACGAAGAATGACAAGGTCTATCTCTGCTCCCCCGAAACGGCA
GCCGCCGCCGGAATCTTCGGCGTGGTCACCGATCCCCGGAAACTCGGCGATCTCATGGCATGGCCCAACGTCCGGAACCC
GGAGAAGTACGTGCTGGACGACTCCAGCATCATCTTCCCCCTGCCGCCCGAGGAGGCGAAGCAGGTGGAGATCGTCACCG
GCCCCAACATCGTCCCGTTTCCCGACTTTGACCCGCTGCCGGAGACCCTGGAGGCCGAGGTCATCTTCATGGTGGGGGAC
AACATCTCCACTGACACCATCATGCCCGCCGGCAACAAGGTGCTTCCCTACCGGAGCAACGTCCCGGCCATCAGCCAGTT
CGTGTTCGAGCAACTGGACCCCGACTTCCACAAGCGGGCCAGGGAGAAGGGGACCGGCGTGGTCATCGGCGGCGAGAACT
ACGGCCAGGGATCGAGCCGTGAGCACGCGGCCCTGGCTCCCCGCTACCTGGGCATCCGGGCCAAGATCGTCAAAAGCTTT
GCGCGGATCCACAAGGCCAATCTGGTCAACTTCGGCATCCTGCCGCTCACGTTCAAGAATCCCGCCGACTATGACCTCCT
GAAGCAGGGGGACCGGCTGGAGTTCCCCGACGTGCGGCGGCTCGTGGCCTCCGGAGCCGTGGAGATCCCGGTGCGGGTGG
GCGGGAAGGAAATCGTCACCCTGCTGGACGTCTCGGACCGGCAGCGGCAGGAACTGCTGGCCGGCGGGACGTTGAACTAC
GTGAAACGGAGCGCCTGA

Upstream 100 bases:

>100_bases
CCATGACCTTCCACGCCCACCCGACCCTCGCCGAGGCCATGAAGGAAGCGGCCCTGGACGTGGAGAAACGGGCGATACAT
GCGTAACTAAGGAGATCCCC

Downstream 100 bases:

>100_bases
CCATGGCCGGACGCGGATATGCCAGTCGCCTGTCGATTCTGTCCGCGTCCGCGCTGATTCTGGCGCTCGTTGTCATCCTC
GCAGCCACCGGAGGCTCCCG

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MGKNLATKILEAHLVKGELTPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAAQYIDHNLLQTDNRNADDHVF
LMTAAQRFGVHLSKPGNGVSHQVHLERFGVPGRTMLGADSHTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVK
LTGQLQPWVSAKDVILEMLRRHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSLFPSDHRTREFLEAQGRG
EVWQELAADPDATYDEYEEIDLATVEPLIACPSSPDNVVRVADIEGLKVDQVLVGSSANSAFRDLMTVCRILDGRRVASH
VSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGCIGMGQAPGTDQVSLRTFPRNFPGRSGTKNDKVYLCSPETA
AAAGIFGVVTDPRKLGDLMAWPNVRNPEKYVLDDSSIIFPLPPEEAKQVEIVTGPNIVPFPDFDPLPETLEAEVIFMVGD
NISTDTIMPAGNKVLPYRSNVPAISQFVFEQLDPDFHKRAREKGTGVVIGGENYGQGSSREHAALAPRYLGIRAKIVKSF
ARIHKANLVNFGILPLTFKNPADYDLLKQGDRLEFPDVRRLVASGAVEIPVRVGGKEIVTLLDVSDRQRQELLAGGTLNY
VKRSA

Sequences:

>Translated_645_residues
MGKNLATKILEAHLVKGELTPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAAQYIDHNLLQTDNRNADDHVF
LMTAAQRFGVHLSKPGNGVSHQVHLERFGVPGRTMLGADSHTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVK
LTGQLQPWVSAKDVILEMLRRHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSLFPSDHRTREFLEAQGRG
EVWQELAADPDATYDEYEEIDLATVEPLIACPSSPDNVVRVADIEGLKVDQVLVGSSANSAFRDLMTVCRILDGRRVASH
VSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGCIGMGQAPGTDQVSLRTFPRNFPGRSGTKNDKVYLCSPETA
AAAGIFGVVTDPRKLGDLMAWPNVRNPEKYVLDDSSIIFPLPPEEAKQVEIVTGPNIVPFPDFDPLPETLEAEVIFMVGD
NISTDTIMPAGNKVLPYRSNVPAISQFVFEQLDPDFHKRAREKGTGVVIGGENYGQGSSREHAALAPRYLGIRAKIVKSF
ARIHKANLVNFGILPLTFKNPADYDLLKQGDRLEFPDVRRLVASGAVEIPVRVGGKEIVTLLDVSDRQRQELLAGGTLNY
VKRSA
>Mature_644_residues
GKNLATKILEAHLVKGELTPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAAQYIDHNLLQTDNRNADDHVFL
MTAAQRFGVHLSKPGNGVSHQVHLERFGVPGRTMLGADSHTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVKL
TGQLQPWVSAKDVILEMLRRHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSLFPSDHRTREFLEAQGRGE
VWQELAADPDATYDEYEEIDLATVEPLIACPSSPDNVVRVADIEGLKVDQVLVGSSANSAFRDLMTVCRILDGRRVASHV
SFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGCIGMGQAPGTDQVSLRTFPRNFPGRSGTKNDKVYLCSPETAA
AAGIFGVVTDPRKLGDLMAWPNVRNPEKYVLDDSSIIFPLPPEEAKQVEIVTGPNIVPFPDFDPLPETLEAEVIFMVGDN
ISTDTIMPAGNKVLPYRSNVPAISQFVFEQLDPDFHKRAREKGTGVVIGGENYGQGSSREHAALAPRYLGIRAKIVKSFA
RIHKANLVNFGILPLTFKNPADYDLLKQGDRLEFPDVRRLVASGAVEIPVRVGGKEIVTLLDVSDRQRQELLAGGTLNYV
KRSA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=717, Percent_Identity=33.7517433751743, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI8659555, Length=817, Percent_Identity=26.0709914320685, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI41352693, Length=744, Percent_Identity=24.7311827956989, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI87081781, Length=678, Percent_Identity=26.6961651917404, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1787531, Length=748, Percent_Identity=27.0053475935829, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=27.8723404255319, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI2367097, Length=432, Percent_Identity=26.3888888888889, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=720, Percent_Identity=34.5833333333333, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI32564738, Length=683, Percent_Identity=34.9926793557833, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25149342, Length=562, Percent_Identity=34.6975088967972, Blast_Score=246, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17568399, Length=765, Percent_Identity=25.4901960784314, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323335, Length=715, Percent_Identity=35.1048951048951, Blast_Score=338, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6322261, Length=734, Percent_Identity=33.6512261580381, Blast_Score=327, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6320440, Length=620, Percent_Identity=27.0967741935484, Blast_Score=163, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321429, Length=750, Percent_Identity=24.6666666666667, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28571643, Length=718, Percent_Identity=32.8690807799443, Blast_Score=318, Evalue=9e-87,
Organism=Drosophila melanogaster, GI281365315, Length=718, Percent_Identity=33.4261838440111, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17864292, Length=718, Percent_Identity=33.4261838440111, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI161076999, Length=685, Percent_Identity=33.7226277372263, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24645686, Length=757, Percent_Identity=27.0805812417437, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17137564, Length=774, Percent_Identity=25.1937984496124, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69646; Mature: 69514

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNLATKILEAHLVKGELTPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAA
CCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHH
QYIDHNLLQTDNRNADDHVFLMTAAQRFGVHLSKPGNGVSHQVHLERFGVPGRTMLGADS
HHHHHHHHCCCCCCCCCEEEEEEEHHHHCEEECCCCCCCCEEEEEECCCCCCCEEECCCC
HTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVKLTGQLQPWVSAKDVILEMLR
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEECEEEECCCCCCCCHHHHHHHHHH
RHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSLFPSDHRTREFLEAQGRG
HHHCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCH
EVWQELAADPDATYDEYEEIDLATVEPLIACPSSPDNVVRVADIEGLKVDQVLVGSSANS
HHHHHHHCCCCCCCHHHHHCCHHCCCHHEECCCCCCCEEEEEECCCCEEEEEEECCCCCH
AFRDLMTVCRILDGRRVASHVSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGC
HHHHHHHHHHHHCCCEEEHEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEE
IGMGQAPGTDQVSLRTFPRNFPGRSGTKNDKVYLCSPETAAAAGIFGVVTDPRKLGDLMA
EECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHCEEEEECCHHHHHHHHC
WPNVRNPEKYVLDDSSIIFPLPPEEAKQVEIVTGPNIVPFPDFDPLPETLEAEVIFMVGD
CCCCCCCCEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
NISTDTIMPAGNKVLPYRSNVPAISQFVFEQLDPDFHKRAREKGTGVVIGGENYGQGSSR
CCCCCEECCCCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCC
EHAALAPRYLGIRAKIVKSFARIHKANLVNFGILPLTFKNPADYDLLKQGDRLEFPDVRR
CCHHHCCHHHHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCCHHHHCCCCCCCCHHHHH
LVASGAVEIPVRVGGKEIVTLLDVSDRQRQELLAGGTLNYVKRSA
HHHCCCEEEEEEECCCEEEEEECCCCHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure 
GKNLATKILEAHLVKGELTPGTEIALKIDHALLQDATGTMAMLEFIAMGVDRVKVELAA
CCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHH
QYIDHNLLQTDNRNADDHVFLMTAAQRFGVHLSKPGNGVSHQVHLERFGVPGRTMLGADS
HHHHHHHHCCCCCCCCCEEEEEEEHHHHCEEECCCCCCCCEEEEEECCCCCCCEEECCCC
HTTSAAGLSMIAIGAGGLDVSLAMAGHPFHLPCPKIWGVKLTGQLQPWVSAKDVILEMLR
CCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEECEEEECCCCCCCCHHHHHHHHHH
RHTVKGGVGKIIEYYGPGVATLSATDRAIIGNMGAELGATTSLFPSDHRTREFLEAQGRG
HHHCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCH
EVWQELAADPDATYDEYEEIDLATVEPLIACPSSPDNVVRVADIEGLKVDQVLVGSSANS
HHHHHHHCCCCCCCHHHHHCCHHCCCHHEECCCCCCCEEEEEECCCCEEEEEEECCCCCH
AFRDLMTVCRILDGRRVASHVSFNVNPGSRQVLENVADQGGIMMLLLAGAQIHQPGCLGC
HHHHHHHHHHHHCCCEEEHEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEE
IGMGQAPGTDQVSLRTFPRNFPGRSGTKNDKVYLCSPETAAAAGIFGVVTDPRKLGDLMA
EECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHCEEEEECCHHHHHHHHC
WPNVRNPEKYVLDDSSIIFPLPPEEAKQVEIVTGPNIVPFPDFDPLPETLEAEVIFMVGD
CCCCCCCCEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
NISTDTIMPAGNKVLPYRSNVPAISQFVFEQLDPDFHKRAREKGTGVVIGGENYGQGSSR
CCCCCEECCCCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCC
EHAALAPRYLGIRAKIVKSFARIHKANLVNFGILPLTFKNPADYDLLKQGDRLEFPDVRR
CCHHHCCHHHHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCCHHHHCCCCCCCCHHHHH
LVASGAVEIPVRVGGKEIVTLLDVSDRQRQELLAGGTLNYVKRSA
HHHCCCEEEEEEECCCEEEEEECCCCHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]