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The map label for this gene is lpdA-1 [H]

Identifier: 39997541

GI number: 39997541

Start: 2682484

End: 2683902

Strand: Reverse

Name: lpdA-1 [H]

Synonym: GSU2446

Alternate gene names: 39997541

Gene position: 2683902-2682484 (Counterclockwise)

Preceding gene: 39997542

Following gene: 39997540

Centisome position: 70.37

GC content: 68.71

Gene sequence:

>1419_bases
ATGGCTGAGCAAACCTTCGACCTTATCGTCATCGGCGCCGGACCCGGCGGCTACGTGGCCGCCATCAGGGCTGCCCAGCT
TGGCATGACCGTGGCGGTGGCGGAACAGCGCGAAACCCTCGGCGGGGTCTGCCTCAACGAGGGGTGCATCCCCAGCAAGG
CGCTCCTGGACTCATCGGAGTTCTTTGCCCAGGCCCGGGACGGCTTTGCCGGCCACGGCATCCTGATTGACCCTCCGCGG
CTCGACCTGGCGCGGATGATGGCCCGCAAGGACGATGTGGTGAAAAAGCTCACCGACGGCATCGCCTACCTCTTCAAGAA
GAACCGGATAACCTGGCTGAAGGGGACGGCTCGCCTGGCGGGCAGAAACGGCGATCTCCTGCGGGTGGAGGTGGGCGGCA
ACGGGACGGCGCCCGCGCACCTGCTGGAGGCCGGCAAGGTGCTCCTTGCCACCGGCAGCGAGGCGGTGCCGGTACCGGGC
CTCGCCTTTGACGGGGAGACCGTGGTCTCCGCCCGGGAAGCCCTGGCCTTCGACCGGGTGCCGGAGCATCTGCTGGTGGT
GGGGGCCGGCTACATCGGGCTGGAGCTGGGGTCGGTCTGGCGCCGGCTCGGCAGCCAGGTGACCGTGGTGGAGGTGCTGG
CCAAGCCGCTCCCCGCCACCGACGGCCAGGTGGCCGACGCTCTGGTGCGCTCCCTGAAGAAGCAGGGGATCTCCTTCCGC
ATGGAGACACGGGTGACCGGCATCGAGAAGCGGGAGGGCACGGCCGTGGTCACGGTGGAATCGTCCGCCGGCGGGCGCGA
CGCCATCGCCTGCGACCGGGTCCTGGTGGCCGCCGGCCGCCGCCCCGTCATGGCCGGGCTCGGCCTCGAACCCTTCAATC
TCGCCATGGAGGGGGGGCGCATCCGGGTGGACGACAACTACCTGACCTCGGCCCCGGGGATCTACGCCATCGGCGACCTG
ATCCACGGACCCATGCTCGCCCACAAGGCCATGGCCGAGGGGGAAGTCTTCGCCGAGCGCCTGACCGGCGAGGCGTCGGT
CGTGGACTACGAATACATCCCTGGTATCGTGTATACGTGGCCCGAGGCGGCCGGCGTCGGCCTGACCGAAGAGCAGCTCA
AGGAACAGGGGATTCCCTATGCCGCGGGCCGGTTCAATTTCATGGCCAACGGTCGGGCACGCTGCATGGGGGAAACCGAA
GGATTCGTGAAGATCCTGGCCAAGCCCGACACGGGCCGGGTCCTGGGCATCCATGTGGTCGGCCCCCGCGCCTCGGACCT
CATTGCCGAGGCGGTCACCGTCATGACCTACGGTGGCAGCGCCGCCGACATCGCCATGACCTTCCACGCCCACCCGACCC
TCGCCGAGGCCATGAAGGAAGCGGCCCTGGACGTGGAGAAACGGGCGATACATGCGTAA

Upstream 100 bases:

>100_bases
ATGGTTGAAACAATAACAGGTTTTCCTTTTTGACCTCTGCGACCTCTGTGGGTAACGGCTTTTTTCAGGATAACTCTACT
TCTCTCCGGGAGGATTTCCC

Downstream 100 bases:

>100_bases
CTAAGGAGATCCCCATGGGCAAGAACCTCGCAACCAAAATCCTCGAAGCGCACCTCGTGAAGGGCGAACTGACGCCGGGC
ACCGAGATCGCCCTGAAGAT

Product: 2-oxoglutarate dehydrogenase complex, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAEQTFDLIVIGAGPGGYVAAIRAAQLGMTVAVAEQRETLGGVCLNEGCIPSKALLDSSEFFAQARDGFAGHGILIDPPR
LDLARMMARKDDVVKKLTDGIAYLFKKNRITWLKGTARLAGRNGDLLRVEVGGNGTAPAHLLEAGKVLLATGSEAVPVPG
LAFDGETVVSAREALAFDRVPEHLLVVGAGYIGLELGSVWRRLGSQVTVVEVLAKPLPATDGQVADALVRSLKKQGISFR
METRVTGIEKREGTAVVTVESSAGGRDAIACDRVLVAAGRRPVMAGLGLEPFNLAMEGGRIRVDDNYLTSAPGIYAIGDL
IHGPMLAHKAMAEGEVFAERLTGEASVVDYEYIPGIVYTWPEAAGVGLTEEQLKEQGIPYAAGRFNFMANGRARCMGETE
GFVKILAKPDTGRVLGIHVVGPRASDLIAEAVTVMTYGGSAADIAMTFHAHPTLAEAMKEAALDVEKRAIHA

Sequences:

>Translated_472_residues
MAEQTFDLIVIGAGPGGYVAAIRAAQLGMTVAVAEQRETLGGVCLNEGCIPSKALLDSSEFFAQARDGFAGHGILIDPPR
LDLARMMARKDDVVKKLTDGIAYLFKKNRITWLKGTARLAGRNGDLLRVEVGGNGTAPAHLLEAGKVLLATGSEAVPVPG
LAFDGETVVSAREALAFDRVPEHLLVVGAGYIGLELGSVWRRLGSQVTVVEVLAKPLPATDGQVADALVRSLKKQGISFR
METRVTGIEKREGTAVVTVESSAGGRDAIACDRVLVAAGRRPVMAGLGLEPFNLAMEGGRIRVDDNYLTSAPGIYAIGDL
IHGPMLAHKAMAEGEVFAERLTGEASVVDYEYIPGIVYTWPEAAGVGLTEEQLKEQGIPYAAGRFNFMANGRARCMGETE
GFVKILAKPDTGRVLGIHVVGPRASDLIAEAVTVMTYGGSAADIAMTFHAHPTLAEAMKEAALDVEKRAIHA
>Mature_471_residues
AEQTFDLIVIGAGPGGYVAAIRAAQLGMTVAVAEQRETLGGVCLNEGCIPSKALLDSSEFFAQARDGFAGHGILIDPPRL
DLARMMARKDDVVKKLTDGIAYLFKKNRITWLKGTARLAGRNGDLLRVEVGGNGTAPAHLLEAGKVLLATGSEAVPVPGL
AFDGETVVSAREALAFDRVPEHLLVVGAGYIGLELGSVWRRLGSQVTVVEVLAKPLPATDGQVADALVRSLKKQGISFRM
ETRVTGIEKREGTAVVTVESSAGGRDAIACDRVLVAAGRRPVMAGLGLEPFNLAMEGGRIRVDDNYLTSAPGIYAIGDLI
HGPMLAHKAMAEGEVFAERLTGEASVVDYEYIPGIVYTWPEAAGVGLTEEQLKEQGIPYAAGRFNFMANGRARCMGETEG
FVKILAKPDTGRVLGIHVVGPRASDLIAEAVTVMTYGGSAADIAMTFHAHPTLAEAMKEAALDVEKRAIHA

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=47.0842332613391, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=27.6231263383298, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=27.7542372881356, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI148277065, Length=474, Percent_Identity=26.7932489451477, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI33519430, Length=474, Percent_Identity=26.7932489451477, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI33519428, Length=474, Percent_Identity=26.7932489451477, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI33519426, Length=474, Percent_Identity=26.7932489451477, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI148277071, Length=474, Percent_Identity=26.7932489451477, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291045266, Length=463, Percent_Identity=25.9179265658747, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI291045268, Length=454, Percent_Identity=24.8898678414097, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=38.646288209607, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=30.9012875536481, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=28.4796573875803, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=441, Percent_Identity=26.3038548752834, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=46.6522678185745, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=25.7142857142857, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=27.3706896551724, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=464, Percent_Identity=27.3706896551724, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=500, Percent_Identity=23.8, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=44.6764091858038, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=31.4893617021277, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6325166, Length=472, Percent_Identity=25.4237288135593, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=46.8409586056645, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=28.0082987551867, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24640553, Length=482, Percent_Identity=28.0082987551867, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24640551, Length=482, Percent_Identity=28.0082987551867, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=25.8726899383984, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49837; Mature: 49706

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQTFDLIVIGAGPGGYVAAIRAAQLGMTVAVAEQRETLGGVCLNEGCIPSKALLDSSE
CCCCEEEEEEEECCCCCHHHHHHHHHHCEEEEEEHHHHHHCCEEECCCCCCHHHHHCCHH
FFAQARDGFAGHGILIDPPRLDLARMMARKDDVVKKLTDGIAYLFKKNRITWLKGTARLA
HHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEC
GRNGDLLRVEVGGNGTAPAHLLEAGKVLLATGSEAVPVPGLAFDGETVVSAREALAFDRV
CCCCCEEEEEECCCCCCHHHHHHCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHCC
PEHLLVVGAGYIGLELGSVWRRLGSQVTVVEVLAKPLPATDGQVADALVRSLKKQGISFR
CCEEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEE
METRVTGIEKREGTAVVTVESSAGGRDAIACDRVLVAAGRRPVMAGLGLEPFNLAMEGGR
EEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHEEEECCE
IRVDDNYLTSAPGIYAIGDLIHGPMLAHKAMAEGEVFAERLTGEASVVDYEYIPGIVYTW
EEECCCEECCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCEEEEC
PEAAGVGLTEEQLKEQGIPYAAGRFNFMANGRARCMGETEGFVKILAKPDTGRVLGIHVV
CCCCCCCCCHHHHHHCCCCEECCCEEEEECCCEEEECCCCCEEEEEECCCCCCEEEEEEE
GPRASDLIAEAVTVMTYGGSAADIAMTFHAHPTLAEAMKEAALDVEKRAIHA
CCCHHHHHHHHHHEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEQTFDLIVIGAGPGGYVAAIRAAQLGMTVAVAEQRETLGGVCLNEGCIPSKALLDSSE
CCCEEEEEEEECCCCCHHHHHHHHHHCEEEEEEHHHHHHCCEEECCCCCCHHHHHCCHH
FFAQARDGFAGHGILIDPPRLDLARMMARKDDVVKKLTDGIAYLFKKNRITWLKGTARLA
HHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEC
GRNGDLLRVEVGGNGTAPAHLLEAGKVLLATGSEAVPVPGLAFDGETVVSAREALAFDRV
CCCCCEEEEEECCCCCCHHHHHHCCCEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHCC
PEHLLVVGAGYIGLELGSVWRRLGSQVTVVEVLAKPLPATDGQVADALVRSLKKQGISFR
CCEEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEE
METRVTGIEKREGTAVVTVESSAGGRDAIACDRVLVAAGRRPVMAGLGLEPFNLAMEGGR
EEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHEEEECCE
IRVDDNYLTSAPGIYAIGDLIHGPMLAHKAMAEGEVFAERLTGEASVVDYEYIPGIVYTW
EEECCCEECCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCEEEEC
PEAAGVGLTEEQLKEQGIPYAAGRFNFMANGRARCMGETEGFVKILAKPDTGRVLGIHVV
CCCCCCCCCHHHHHHCCCCEECCCEEEEECCCEEEECCCCCEEEEEECCCCCCEEEEEEE
GPRASDLIAEAVTVMTYGGSAADIAMTFHAHPTLAEAMKEAALDVEKRAIHA
CCCHHHHHHHHHHEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]