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The map label for this gene is rfbD [H]

Identifier: 39997461

GI number: 39997461

Start: 2591704

End: 2592546

Strand: Reverse

Name: rfbD [H]

Synonym: GSU2365

Alternate gene names: 39997461

Gene position: 2592546-2591704 (Counterclockwise)

Preceding gene: 39997462

Following gene: 39997460

Centisome position: 67.97

GC content: 64.06

Gene sequence:

>843_bases
ATGATCCTGGTGGTCGGTGCCAAGGGAATGCTCGGCCGCGACCTCATGCGGGTGCTGCCGGGGGACGTGCGGGGGGTCGA
TATCGAAGAGATCGACATTACCTCGCCGGAATCGGTACGGCGGGTCATTCTGACCCTGAAGCCGCGGGTGGTGGTGAACT
GTGCCGCCTACACCGATGTGGACGGCTGCGAGACCAACGCGGACCTTGCCATGGCGGTGAACGGCGAGGGGGTGGGGCAC
CTGGCCGCCGTGACCCGCGAGATCGGGGCGCTGCTCGTTCAGATGAGCACCGATTACGTGTTCGACGGCGTCAAGGAGAG
CCCGTTTCTGGAGGATGATCCGCCCAATCCCCTGAGCGTGTACGGCAGATCGAAGCTTATGGGCGAGGAACAGGCCCGTG
AAACCCCCGATCACCTCATCGTCCGGACCCAGTGGCTCTATGGGCTCGGCGGCAAGAACTTCGTGGAAACCATGCTGCGC
CTGTCGACGGAGCGGAGCGAAATCGCCGTGGTGGACGACCAGATCGGCTCCCCCACCTGGACAGTGGACCTTGCCCTGGC
CATCAGCGAACTGATAGAGAACAACTGCCGCGGCACCTATCATGCGGCCAACAGGGGGATCTGTTCCTGGTTCGATTTCG
CCCGCGCCATTTTTGCCGAAGCAGGAGTGGAGATGACCGTCCGGCCCCAGACCACCGCCCAACTGGGCCGGCCGGCCCCG
CGTCCCCTCTATTCGGCTCTCTGCTGCGACAAGTTGACCCGGGACACGGGGCTCGAACTGGAAGGCTGGCGCGAGGCACT
TGCCACCTATTTGGAAAAGCGGCGCGAAGCCGGATTGTCCTGA

Upstream 100 bases:

>100_bases
CCGAAACCGTGGACTGGTACCTGGCCAACCGCGCCTGGGTGGATGAAGTGACGTCCGGCTCATACCGGGATTACTACGAA
ACACAGTACGGAGGCGGGCA

Downstream 100 bases:

>100_bases
CGGAGGAACACGACGATGGAACTGGAGCGCGGCGAACGTCCTTGGGGCACATACACCGTTCTCGAGGAGAACAAGAACTA
CAAGATCAAGCGGATCGAGG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MILVVGAKGMLGRDLMRVLPGDVRGVDIEEIDITSPESVRRVILTLKPRVVVNCAAYTDVDGCETNADLAMAVNGEGVGH
LAAVTREIGALLVQMSTDYVFDGVKESPFLEDDPPNPLSVYGRSKLMGEEQARETPDHLIVRTQWLYGLGGKNFVETMLR
LSTERSEIAVVDDQIGSPTWTVDLALAISELIENNCRGTYHAANRGICSWFDFARAIFAEAGVEMTVRPQTTAQLGRPAP
RPLYSALCCDKLTRDTGLELEGWREALATYLEKRREAGLS

Sequences:

>Translated_280_residues
MILVVGAKGMLGRDLMRVLPGDVRGVDIEEIDITSPESVRRVILTLKPRVVVNCAAYTDVDGCETNADLAMAVNGEGVGH
LAAVTREIGALLVQMSTDYVFDGVKESPFLEDDPPNPLSVYGRSKLMGEEQARETPDHLIVRTQWLYGLGGKNFVETMLR
LSTERSEIAVVDDQIGSPTWTVDLALAISELIENNCRGTYHAANRGICSWFDFARAIFAEAGVEMTVRPQTTAQLGRPAP
RPLYSALCCDKLTRDTGLELEGWREALATYLEKRREAGLS
>Mature_280_residues
MILVVGAKGMLGRDLMRVLPGDVRGVDIEEIDITSPESVRRVILTLKPRVVVNCAAYTDVDGCETNADLAMAVNGEGVGH
LAAVTREIGALLVQMSTDYVFDGVKESPFLEDDPPNPLSVYGRSKLMGEEQARETPDHLIVRTQWLYGLGGKNFVETMLR
LSTERSEIAVVDDQIGSPTWTVDLALAISELIENNCRGTYHAANRGICSWFDFARAIFAEAGVEMTVRPQTTAQLGRPAP
RPLYSALCCDKLTRDTGLELEGWREALATYLEKRREAGLS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=273, Percent_Identity=30.03663003663, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI11034825, Length=273, Percent_Identity=30.03663003663, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788352, Length=281, Percent_Identity=35.9430604982206, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 30730; Mature: 30730

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVVGAKGMLGRDLMRVLPGDVRGVDIEEIDITSPESVRRVILTLKPRVVVNCAAYTDV
CEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHEECCCEEEEEEEECCC
DGCETNADLAMAVNGEGVGHLAAVTREIGALLVQMSTDYVFDGVKESPFLEDDPPNPLSV
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHH
YGRSKLMGEEQARETPDHLIVRTQWLYGLGGKNFVETMLRLSTERSEIAVVDDQIGSPTW
CCHHHHCCCHHHHCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEE
TVDLALAISELIENNCRGTYHAANRGICSWFDFARAIFAEAGVEMTVRPQTTAQLGRPAP
EHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCC
RPLYSALCCDKLTRDTGLELEGWREALATYLEKRREAGLS
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILVVGAKGMLGRDLMRVLPGDVRGVDIEEIDITSPESVRRVILTLKPRVVVNCAAYTDV
CEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHEECCCEEEEEEEECCC
DGCETNADLAMAVNGEGVGHLAAVTREIGALLVQMSTDYVFDGVKESPFLEDDPPNPLSV
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHH
YGRSKLMGEEQARETPDHLIVRTQWLYGLGGKNFVETMLRLSTERSEIAVVDDQIGSPTW
CCHHHHCCCHHHHCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEE
TVDLALAISELIENNCRGTYHAANRGICSWFDFARAIFAEAGVEMTVRPQTTAQLGRPAP
EHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCC
RPLYSALCCDKLTRDTGLELEGWREALATYLEKRREAGLS
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]