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The map label for this gene is rfbB [H]

Identifier: 39997462

GI number: 39997462

Start: 2592543

End: 2593619

Strand: Reverse

Name: rfbB [H]

Synonym: GSU2366

Alternate gene names: 39997462

Gene position: 2593619-2592543 (Counterclockwise)

Preceding gene: 308513319

Following gene: 39997461

Centisome position: 68.0

GC content: 62.3

Gene sequence:

>1077_bases
ATGGGTGACACCTTTTCCCCTTCCGCCGTGCTCGTGACCGGCGGCGCCGGCTTCATCGGCTCCAATTTCATTAATCACTT
CCTGCCGGCGAACCCCGGTTGCCGGGTGATCAATCTGGATATCCTCACCTATGCCGGCAATCTGCGCAATTTGACAGCCG
TGGAGCAGAACCCGGCCTACCGCTTCGTTAAGGGCGACATCGGCGATGCCGACCTCGTCCGGCGCATCCTGGCCGAAGAG
CGGATCGACGCGGTTGTCCACTTTGCCGCCGAATCCCACGTGGACCGCTCCATCCTGGGACCGGAAATTTTCGTGAGAAC
CAACGTCCTCGGCACCCAGGTGCTCCTGGAGGAAAGCCGCCGGCACTGGGAATCGGGCGCGATCGAGCGCTTCCGCTTCC
TGCATGTTTCGACCGACGAGGTGTACGGGACACTGGGGGAAACGGGCTACTTCACCGAGGAGACCCCCCTGGCCCCCAAC
TCCCCCTACTCGGCGAGCAAGGCCGGTTCCGACCTGCTGGTGCGGGCCTACAACGAGACGTTCGGCCTGCCGGTCCTGAC
CACCCGCTGCTCCAACAACTACGGTCCCTTCCAGTTTCCGGAGAAACTGATCCCGCTCATGATCCACAATATCGTGGCCG
GAAAGCCCCTGCCGGTGTACGGCGACGGCCGCAACGTGCGCGACTGGCTCCATGTGAAGGATCATTCCACGGCCATCGAA
ACGGTGCTCAAGGGTGGCAAGCCGGGCGAGGTCTTCAACGTGGGCGGCAACAACGAGTGGTTCAACATCGACATCGTTCA
CCTGCTCTGCGATCTCCTGGATGAACGGCTCGGCAGACCCGGCGGGGAAAGCCGCGGCCTGATCACCTTTGTCAAGGATC
GTCTCGGCCATGATCGCCGCTACGCCATCAGCGCTGCCAAAATCAAGCGGGAGCTGGGATGGGAGCCCTCGTACACCTTC
GAGCGCGGCATTGCCGAAACCGTGGACTGGTACCTGGCCAACCGCGCCTGGGTGGATGAAGTGACGTCCGGCTCATACCG
GGATTACTACGAAACACAGTACGGAGGCGGGCAATGA

Upstream 100 bases:

>100_bases
CGCGACCGGCCCGATACCAACGAATTCTTTGTTACCTTTGCCCTGGCCGGTATCGGTTCAGTGGGCAAGATACGGGCATT
CTAATTCTCAGGAGGTTGTC

Downstream 100 bases:

>100_bases
TCCTGGTGGTCGGTGCCAAGGGAATGCTCGGCCGCGACCTCATGCGGGTGCTGCCGGGGGACGTGCGGGGGGTCGATATC
GAAGAGATCGACATTACCTC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MGDTFSPSAVLVTGGAGFIGSNFINHFLPANPGCRVINLDILTYAGNLRNLTAVEQNPAYRFVKGDIGDADLVRRILAEE
RIDAVVHFAAESHVDRSILGPEIFVRTNVLGTQVLLEESRRHWESGAIERFRFLHVSTDEVYGTLGETGYFTEETPLAPN
SPYSASKAGSDLLVRAYNETFGLPVLTTRCSNNYGPFQFPEKLIPLMIHNIVAGKPLPVYGDGRNVRDWLHVKDHSTAIE
TVLKGGKPGEVFNVGGNNEWFNIDIVHLLCDLLDERLGRPGGESRGLITFVKDRLGHDRRYAISAAKIKRELGWEPSYTF
ERGIAETVDWYLANRAWVDEVTSGSYRDYYETQYGGGQ

Sequences:

>Translated_358_residues
MGDTFSPSAVLVTGGAGFIGSNFINHFLPANPGCRVINLDILTYAGNLRNLTAVEQNPAYRFVKGDIGDADLVRRILAEE
RIDAVVHFAAESHVDRSILGPEIFVRTNVLGTQVLLEESRRHWESGAIERFRFLHVSTDEVYGTLGETGYFTEETPLAPN
SPYSASKAGSDLLVRAYNETFGLPVLTTRCSNNYGPFQFPEKLIPLMIHNIVAGKPLPVYGDGRNVRDWLHVKDHSTAIE
TVLKGGKPGEVFNVGGNNEWFNIDIVHLLCDLLDERLGRPGGESRGLITFVKDRLGHDRRYAISAAKIKRELGWEPSYTF
ERGIAETVDWYLANRAWVDEVTSGSYRDYYETQYGGGQ
>Mature_357_residues
GDTFSPSAVLVTGGAGFIGSNFINHFLPANPGCRVINLDILTYAGNLRNLTAVEQNPAYRFVKGDIGDADLVRRILAEER
IDAVVHFAAESHVDRSILGPEIFVRTNVLGTQVLLEESRRHWESGAIERFRFLHVSTDEVYGTLGETGYFTEETPLAPNS
PYSASKAGSDLLVRAYNETFGLPVLTTRCSNNYGPFQFPEKLIPLMIHNIVAGKPLPVYGDGRNVRDWLHVKDHSTAIET
VLKGGKPGEVFNVGGNNEWFNIDIVHLLCDLLDERLGRPGGESRGLITFVKDRLGHDRRYAISAAKIKRELGWEPSYTFE
RGIAETVDWYLANRAWVDEVTSGSYRDYYETQYGGGQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=39.2638036809816, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI42516563, Length=328, Percent_Identity=24.0853658536585, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=54.2936288088643, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=54.4927536231884, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=27.5568181818182, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=29.3785310734463, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=330, Percent_Identity=36.0606060606061, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=34.9544072948328, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17539532, Length=342, Percent_Identity=24.5614035087719, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=340, Percent_Identity=24.1176470588235, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=280, Percent_Identity=25.3571428571429, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=28.099173553719, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=27.2479564032698, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21356223, Length=350, Percent_Identity=23.4285714285714, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39857; Mature: 39726

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTFSPSAVLVTGGAGFIGSNFINHFLPANPGCRVINLDILTYAGNLRNLTAVEQNPAY
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEECCCCCE
RFVKGDIGDADLVRRILAEERIDAVVHFAAESHVDRSILGPEIFVRTNVLGTQVLLEESR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHH
RHWESGAIERFRFLHVSTDEVYGTLGETGYFTEETPLAPNSPYSASKAGSDLLVRAYNET
HHHHCCCCEEEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
FGLPVLTTRCSNNYGPFQFPEKLIPLMIHNIVAGKPLPVYGDGRNVRDWLHVKDHSTAIE
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECCCHHHHH
TVLKGGKPGEVFNVGGNNEWFNIDIVHLLCDLLDERLGRPGGESRGLITFVKDRLGHDRR
HHHCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHH
YAISAAKIKRELGWEPSYTFERGIAETVDWYLANRAWVDEVTSGSYRDYYETQYGGGQ
EEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
GDTFSPSAVLVTGGAGFIGSNFINHFLPANPGCRVINLDILTYAGNLRNLTAVEQNPAY
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEECCCCCE
RFVKGDIGDADLVRRILAEERIDAVVHFAAESHVDRSILGPEIFVRTNVLGTQVLLEESR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCHHHHHHHHHHH
RHWESGAIERFRFLHVSTDEVYGTLGETGYFTEETPLAPNSPYSASKAGSDLLVRAYNET
HHHHCCCCEEEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
FGLPVLTTRCSNNYGPFQFPEKLIPLMIHNIVAGKPLPVYGDGRNVRDWLHVKDHSTAIE
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECCCHHHHH
TVLKGGKPGEVFNVGGNNEWFNIDIVHLLCDLLDERLGRPGGESRGLITFVKDRLGHDRR
HHHCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHH
YAISAAKIKRELGWEPSYTFERGIAETVDWYLANRAWVDEVTSGSYRDYYETQYGGGQ
EEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]