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The map label for this gene is mrpA [H]

Identifier: 39997440

GI number: 39997440

Start: 2561188

End: 2563482

Strand: Reverse

Name: mrpA [H]

Synonym: GSU2344

Alternate gene names: 39997440

Gene position: 2563482-2561188 (Counterclockwise)

Preceding gene: 39997441

Following gene: 39997439

Centisome position: 67.21

GC content: 67.36

Gene sequence:

>2295_bases
ATGATTATTGCCGCGTTATCAGGATTTGGAGCCGCCCTGGCCGCTCCTCTGCTCCACCGTTGGCTCCCGCGGTGGGCCGG
GTGGCTGTGCGCCCTGGTGCCCCTGTCGCTGTTCAGCTTTTTCGGCGCACAGGTCGGGGCGGTGGCCTCCGGCAGAACGA
TCCGGGCCGCCTACGAGTGGATTCCGTCCCTGGGGGTCGACCTTTCCTTCTCCCTCGACGGCCTGAGCCTCCTGTTCGTC
CTGATCATCAGCGGCATCGGCGTGCTCGTGGTGGTCTACGCCTCTTCGTACCTCCGCGGCCATCACCACCTGGGGCGTTT
CTACGGCCTGATCCTCGCGTTCATGGCCGCCATGATCAGCACGGTCCTGGCCGGCGATCTGATTACCCTTTTTGTCTTCT
GGGAGCTGACCGGTCTCTGCTCCTACCTCCTGATCGGCTTCGACCACGGCCGGATGGCGGCGCGCAAGGCGGCGCTCCAG
GCATTGCTGGTGACCGGCGCCGGCGGGCTGGCGCTTCTGGCCGGGTTCATCCTGCTGGGGCAGGCGGCGGGAAGCACGGA
CCTGGCCGAGATCTTCCAGAGGGGCGAACTGGTGCGGGGCCATGCACTCTATTTGCCGATCCTTTTCCTGGTTCTGGCCG
GGGCATTTACCAAGTCGGCCCAATTCCCCTTCCACTTCTGGCTCCCGGGCGCCATGGAGGCCCCGACGCCGGTCAGCGCC
TATCTCCATTCGGCCACCATGGTGAAATTGGGGATTTATCTCCTGGCACGCCTCTCCCCGGTCCTGGGCGGGACCGATGC
CTGGTTCTGGTCGGTGACCCTGGCCGGCGGGGTTACCATGGTGGCGGGCGGGGCCCTGGCCCTGCTCCGGAGCGATCTGA
AGCAGATTCTCGCCTATTCCACGGTGAGCGCCCTGGGGATTCTGACGCTGCTGCTGGGGCTCCATACGCCGGCCGCCGCA
ACGGCCGCCATGGTCTTTCTCCTGGTCCACGCCCTTTACAAGAGTGCCCTGTTCCTGGCGGCCGGAGCTGTGGATCACGG
TGCCGGAACCCGCGACCTGGGGCGGCTCGGCGGATTGGCGCGGGTCATGCCCTGGGTCGCCGCCGGATCGGGTCTCGCTG
CCCTCTCCATGGCCGGGGTGCCGCCGCTGGTGGGGTTCATCGCCAAGGAACTCCTCTACGAGGCCAAGCTCCAGGCACCC
CTGGCGGCAGGATTCGTGACTGTTGCCGGCTTCATCGGCAATGCCTTGACCGTGGCGGTTGCGGTCCTGGTGGGGTATCT
CCCCTTTTTCGGGCGCGGGACCGATCCGGATCTGCACCACCACCGGGTGGAGCCGCTCCTCTGGTTCCCCCCCCTGGTGC
CGGCCCTGCTGGGTTTTGTGATCGGCATCTTCCCGGGCCTGGCGGGACGATGGTTGCTGGAGCCGGCAGTGGCCATGGTG
CGGGGGGCATCGGTGCCGGTGGAGCTGGCCCTCTGGCATGGGGTGACTCCAGTCCTCCTTCTTGGAGCGGCCACGGTGGC
CACCGGGGTCGGCATCGCCCTTGTCCGGGAAACGGTGCTTCGTCGCCTTCCCCTCGGTGACGGCGCGGGATGGGGCCCCG
AACGGGGCTACGGGCTCCTCCTGAAGGGAATGCTCGCCCTGGCCGAACGGCAGACCCGCATGCTTCAGAACGGCCGGCTC
CGCTATTACCTGATGACGGTGGTGGGGACCGCGGCGTTCCTCATGGCCGTGGCCATTTTCCGGAGTGGGGAGTTCCCGCG
CCAGCTTCCCCGACCGGACGGCGGGCTCCACGAATGGTTCGCCGCCGGCGTCATCATGGCAGGAGCACTGACGGCGGCGA
CGACCACCTCTCGGCTGGCGGCGGTGGCCGGGCTCGGCGCGGTGGGGTACGGGGTGGCCCTGGTCTATATCCTTTTCGGC
GCCCCGGACCTGGCCATGACCCAGTTCTGTATCGAGACCCTCTCCATCGTCCTGTTCGTTCTGGTCCTCTACCGGCTGCC
GCGCTTCTCGCTGCTCTCGCGGCCGGCGGGAAGGGTGCGGGATATCCTGGTGGCCTGCTCCATCGGCGGCTTCATGACGG
TGCTGGTCCTGTTGGCGGTATCCCAGACCCTGGTCCCCCACATCAGCTCCTATTTTCTGGCGCACAGCCTGCCGGCGGGG
CATGGCCGCAACGTGGTCAACGTGATCCTGGTGGATTTCCGGGCACTGGATACCCTGGGGGAGATCACAGTCCTGGCCGT
TGCGGCCCTGGGGGTCTACGGCCTCATGAAGGCCCGGAACAGGGAGGAGGGCTAG

Upstream 100 bases:

>100_bases
TCATCCGTGCCGGGTGTCATAATCCGGTCGTTTCCTTGCAGCCGCCGGCTCCACCGTTAGAATGGAGTAAACGCACGTGT
CGCTGCCGACGGGTTTATCT

Downstream 100 bases:

>100_bases
GCCATGCAGTCACTCTTTCTGGCAACAGCGGTCCGGCTTCTCCTGCCGCTGCTGCTCCTCTTCTCGCTCTTTCTTCTCCT
GCGCGGGCACAATGAGCCGG

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MIIAALSGFGAALAAPLLHRWLPRWAGWLCALVPLSLFSFFGAQVGAVASGRTIRAAYEWIPSLGVDLSFSLDGLSLLFV
LIISGIGVLVVVYASSYLRGHHHLGRFYGLILAFMAAMISTVLAGDLITLFVFWELTGLCSYLLIGFDHGRMAARKAALQ
ALLVTGAGGLALLAGFILLGQAAGSTDLAEIFQRGELVRGHALYLPILFLVLAGAFTKSAQFPFHFWLPGAMEAPTPVSA
YLHSATMVKLGIYLLARLSPVLGGTDAWFWSVTLAGGVTMVAGGALALLRSDLKQILAYSTVSALGILTLLLGLHTPAAA
TAAMVFLLVHALYKSALFLAAGAVDHGAGTRDLGRLGGLARVMPWVAAGSGLAALSMAGVPPLVGFIAKELLYEAKLQAP
LAAGFVTVAGFIGNALTVAVAVLVGYLPFFGRGTDPDLHHHRVEPLLWFPPLVPALLGFVIGIFPGLAGRWLLEPAVAMV
RGASVPVELALWHGVTPVLLLGAATVATGVGIALVRETVLRRLPLGDGAGWGPERGYGLLLKGMLALAERQTRMLQNGRL
RYYLMTVVGTAAFLMAVAIFRSGEFPRQLPRPDGGLHEWFAAGVIMAGALTAATTTSRLAAVAGLGAVGYGVALVYILFG
APDLAMTQFCIETLSIVLFVLVLYRLPRFSLLSRPAGRVRDILVACSIGGFMTVLVLLAVSQTLVPHISSYFLAHSLPAG
HGRNVVNVILVDFRALDTLGEITVLAVAALGVYGLMKARNREEG

Sequences:

>Translated_764_residues
MIIAALSGFGAALAAPLLHRWLPRWAGWLCALVPLSLFSFFGAQVGAVASGRTIRAAYEWIPSLGVDLSFSLDGLSLLFV
LIISGIGVLVVVYASSYLRGHHHLGRFYGLILAFMAAMISTVLAGDLITLFVFWELTGLCSYLLIGFDHGRMAARKAALQ
ALLVTGAGGLALLAGFILLGQAAGSTDLAEIFQRGELVRGHALYLPILFLVLAGAFTKSAQFPFHFWLPGAMEAPTPVSA
YLHSATMVKLGIYLLARLSPVLGGTDAWFWSVTLAGGVTMVAGGALALLRSDLKQILAYSTVSALGILTLLLGLHTPAAA
TAAMVFLLVHALYKSALFLAAGAVDHGAGTRDLGRLGGLARVMPWVAAGSGLAALSMAGVPPLVGFIAKELLYEAKLQAP
LAAGFVTVAGFIGNALTVAVAVLVGYLPFFGRGTDPDLHHHRVEPLLWFPPLVPALLGFVIGIFPGLAGRWLLEPAVAMV
RGASVPVELALWHGVTPVLLLGAATVATGVGIALVRETVLRRLPLGDGAGWGPERGYGLLLKGMLALAERQTRMLQNGRL
RYYLMTVVGTAAFLMAVAIFRSGEFPRQLPRPDGGLHEWFAAGVIMAGALTAATTTSRLAAVAGLGAVGYGVALVYILFG
APDLAMTQFCIETLSIVLFVLVLYRLPRFSLLSRPAGRVRDILVACSIGGFMTVLVLLAVSQTLVPHISSYFLAHSLPAG
HGRNVVNVILVDFRALDTLGEITVLAVAALGVYGLMKARNREEG
>Mature_764_residues
MIIAALSGFGAALAAPLLHRWLPRWAGWLCALVPLSLFSFFGAQVGAVASGRTIRAAYEWIPSLGVDLSFSLDGLSLLFV
LIISGIGVLVVVYASSYLRGHHHLGRFYGLILAFMAAMISTVLAGDLITLFVFWELTGLCSYLLIGFDHGRMAARKAALQ
ALLVTGAGGLALLAGFILLGQAAGSTDLAEIFQRGELVRGHALYLPILFLVLAGAFTKSAQFPFHFWLPGAMEAPTPVSA
YLHSATMVKLGIYLLARLSPVLGGTDAWFWSVTLAGGVTMVAGGALALLRSDLKQILAYSTVSALGILTLLLGLHTPAAA
TAAMVFLLVHALYKSALFLAAGAVDHGAGTRDLGRLGGLARVMPWVAAGSGLAALSMAGVPPLVGFIAKELLYEAKLQAP
LAAGFVTVAGFIGNALTVAVAVLVGYLPFFGRGTDPDLHHHRVEPLLWFPPLVPALLGFVIGIFPGLAGRWLLEPAVAMV
RGASVPVELALWHGVTPVLLLGAATVATGVGIALVRETVLRRLPLGDGAGWGPERGYGLLLKGMLALAERQTRMLQNGRL
RYYLMTVVGTAAFLMAVAIFRSGEFPRQLPRPDGGLHEWFAAGVIMAGALTAATTTSRLAAVAGLGAVGYGVALVYILFG
APDLAMTQFCIETLSIVLFVLVLYRLPRFSLLSRPAGRVRDILVACSIGGFMTVLVLLAVSQTLVPHISSYFLAHSLPAG
HGRNVVNVILVDFRALDTLGEITVLAVAALGVYGLMKARNREEG

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=422, Percent_Identity=30.5687203791469, Blast_Score=177, Evalue=5e-44,
Organism=Homo sapiens, GI251831116, Length=291, Percent_Identity=28.1786941580756, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788614, Length=433, Percent_Identity=33.256351039261, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788829, Length=340, Percent_Identity=37.0588235294118, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788831, Length=346, Percent_Identity=31.2138728323699, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788827, Length=349, Percent_Identity=30.3724928366762, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1788613, Length=350, Percent_Identity=28, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI2367154, Length=433, Percent_Identity=29.3302540415704, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI145693160, Length=425, Percent_Identity=25.8823529411765, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 80385; Mature: 80385

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIAALSGFGAALAAPLLHRWLPRWAGWLCALVPLSLFSFFGAQVGAVASGRTIRAAYEW
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IPSLGVDLSFSLDGLSLLFVLIISGIGVLVVVYASSYLRGHHHLGRFYGLILAFMAAMIS
HHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLAGDLITLFVFWELTGLCSYLLIGFDHGRMAARKAALQALLVTGAGGLALLAGFILLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QAAGSTDLAEIFQRGELVRGHALYLPILFLVLAGAFTKSAQFPFHFWLPGAMEAPTPVSA
CCCCCHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHH
YLHSATMVKLGIYLLARLSPVLGGTDAWFWSVTLAGGVTMVAGGALALLRSDLKQILAYS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TVSALGILTLLLGLHTPAAATAAMVFLLVHALYKSALFLAAGAVDHGAGTRDLGRLGGLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHH
RVMPWVAAGSGLAALSMAGVPPLVGFIAKELLYEAKLQAPLAAGFVTVAGFIGNALTVAV
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AVLVGYLPFFGRGTDPDLHHHRVEPLLWFPPLVPALLGFVIGIFPGLAGRWLLEPAVAMV
HHHHHHHHHCCCCCCCCCHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RGASVPVELALWHGVTPVLLLGAATVATGVGIALVRETVLRRLPLGDGAGWGPERGYGLL
CCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LKGMLALAERQTRMLQNGRLRYYLMTVVGTAAFLMAVAIFRSGEFPRQLPRPDGGLHEWF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AAGVIMAGALTAATTTSRLAAVAGLGAVGYGVALVYILFGAPDLAMTQFCIETLSIVLFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LVLYRLPRFSLLSRPAGRVRDILVACSIGGFMTVLVLLAVSQTLVPHISSYFLAHSLPAG
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HGRNVVNVILVDFRALDTLGEITVLAVAALGVYGLMKARNREEG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIIAALSGFGAALAAPLLHRWLPRWAGWLCALVPLSLFSFFGAQVGAVASGRTIRAAYEW
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IPSLGVDLSFSLDGLSLLFVLIISGIGVLVVVYASSYLRGHHHLGRFYGLILAFMAAMIS
HHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLAGDLITLFVFWELTGLCSYLLIGFDHGRMAARKAALQALLVTGAGGLALLAGFILLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QAAGSTDLAEIFQRGELVRGHALYLPILFLVLAGAFTKSAQFPFHFWLPGAMEAPTPVSA
CCCCCHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHH
YLHSATMVKLGIYLLARLSPVLGGTDAWFWSVTLAGGVTMVAGGALALLRSDLKQILAYS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TVSALGILTLLLGLHTPAAATAAMVFLLVHALYKSALFLAAGAVDHGAGTRDLGRLGGLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHH
RVMPWVAAGSGLAALSMAGVPPLVGFIAKELLYEAKLQAPLAAGFVTVAGFIGNALTVAV
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AVLVGYLPFFGRGTDPDLHHHRVEPLLWFPPLVPALLGFVIGIFPGLAGRWLLEPAVAMV
HHHHHHHHHCCCCCCCCCHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RGASVPVELALWHGVTPVLLLGAATVATGVGIALVRETVLRRLPLGDGAGWGPERGYGLL
CCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LKGMLALAERQTRMLQNGRLRYYLMTVVGTAAFLMAVAIFRSGEFPRQLPRPDGGLHEWF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AAGVIMAGALTAATTTSRLAAVAGLGAVGYGVALVYILFGAPDLAMTQFCIETLSIVLFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LVLYRLPRFSLLSRPAGRVRDILVACSIGGFMTVLVLLAVSQTLVPHISSYFLAHSLPAG
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HGRNVVNVILVDFRALDTLGEITVLAVAALGVYGLMKARNREEG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]