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The map label for this gene is lysS [H]

Identifier: 39997369

GI number: 39997369

Start: 2487384

End: 2488859

Strand: Reverse

Name: lysS [H]

Synonym: GSU2271

Alternate gene names: 39997369

Gene position: 2488859-2487384 (Counterclockwise)

Preceding gene: 39997370

Following gene: 39997368

Centisome position: 65.25

GC content: 60.5

Gene sequence:

>1476_bases
ATGGAAGAGTTGAGTGAGCTGTTGCTCCAGAGGCGGCGCAAGGTCGACGCCCTCTGGGAAGCGGGGATCAATCCCTACCC
GAATGATTTCAAACCTCTCCATACGTCTGCCGATTTCAGGGAGGCCTATGGCACCGTGGATACCATTGAGGATGATCCCC
GCAGCTTTACCGTCGCGGGCCGTATCATCGCGCGCCGCTCCTTCGGCAAGGCTGCGTTTATCCAGATCCAGGACCGTAAG
GGACGGATGCAGGTCTACGTACGCAAGGATACGGTGGGCGACGAGGCGTTCGAGGCGTTCGAGACTTTCGATATCGGCGA
CATCGTCGGTGTCGGCGGCACCCCGTTCCGGACCAAGACCGGCGAGCTCACTCTCAATGCCGCGTTCATCCGTCTCCTGA
CCAAGTCGCTCCAGCCGCTTCCCGAGAAGTTCCACGGGCTCACGGACGTTGAGACGCGCTATCGCCAGCGCTACGTGGAC
CTCATCGTGAACCCCGAGGTGCGCGAGGTGTTCGTCAAGCGGTCCCGCATCGTCAATCTCATCCGCGAATTCATGGTGCG
CAAGGATTTTCTCGAAGTGGAAACGCCCATGATGCAGCCGATCCCCGGCGGTGCCACGGCGCGCCCCTTCGTGACCCACC
ACAATGCCCTCGATATGCAGCTTTTTCTTCGGATCGCACCGGAGCTGTACCTGAAGCGGCTCGTGGTCGGCGGCTTCGAG
CGGGTCTTCGAGATCAACCGTAACTTCCGCAACGAGGGGATTTCAGTGCGCCACAACCCCGAGTTCACCATGATGGAGTT
CTATCAGGCCTACGCCACCTTTGAAGACCTGATGGATTTCACGGAGGAACTCCTCTGTCATGTTGCGCAGGAAGTGCTGG
GCACCCTCGATTTCACCTACCAGGAGATGCCGATCAGCTTCCAGCGCCCCTGGAAGCGGCTCGCCGTTGTCGAGGCGATT
CTGGAATACGGCGACATCGACGCCAAGTCCCTGGCGGACCGGGATCTGGCCTATGCCTACGCCCAGCGGCTGGGGCTCGA
CCTGCCCGCCGACGTGGGGTACGGCAAGCTCATTACCGAGATTTTCGAGGAAGTGGCGGAAACAAAACTGATCCAGCCGA
CGTTCATTACCGCCTATCCCACCGAAGTGTCACCCCTTTCCCGCAAGAGCGACAAGGATCCGGAGATCGTGGACCGGTTC
GAGCTGTTCATTGCCGGTCGCGAGATGGCCAACGCCTTCTCCGAGTTGAACGACCCGGTGGACCAGAAGGAGCGCTTCCT
CGCCCAGGTGGCGGCCAAGGCCAAGGGCGACGACGAGGCCCACTACATGGACGAGGATTACATCCGCGCGCTTGAGTTCG
GCCTTCCCCCCACGGCGGGGGAGGGGATCGGCATCGACCGGCTCGTGATGCTGCTGACCGATTCACCTTCGATTCGCGAC
GTCATTCTCTTTCCGCAACTACGCAAGGAAAAATAA

Upstream 100 bases:

>100_bases
TCGCACCCCGTTTTTCCTTGACTTGGCATGCTCCTTTGGCTAAGTTGACGGTTTGTTAAACGGGCCGGAAAGCGGCTACC
AGAAAGGGACTGTACGAGCA

Downstream 100 bases:

>100_bases
GAAAACCGGGTCCAGGGGCCGGAGAACGGCAGGCGGATTTCTCCCGGTCCCCGATTTCCGGTTCCGGATGCACCCATGCC
CTACGAGTTATTTATCGGTC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEELSELLLQRRRKVDALWEAGINPYPNDFKPLHTSADFREAYGTVDTIEDDPRSFTVAGRIIARRSFGKAAFIQIQDRK
GRMQVYVRKDTVGDEAFEAFETFDIGDIVGVGGTPFRTKTGELTLNAAFIRLLTKSLQPLPEKFHGLTDVETRYRQRYVD
LIVNPEVREVFVKRSRIVNLIREFMVRKDFLEVETPMMQPIPGGATARPFVTHHNALDMQLFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVAQEVLGTLDFTYQEMPISFQRPWKRLAVVEAI
LEYGDIDAKSLADRDLAYAYAQRLGLDLPADVGYGKLITEIFEEVAETKLIQPTFITAYPTEVSPLSRKSDKDPEIVDRF
ELFIAGREMANAFSELNDPVDQKERFLAQVAAKAKGDDEAHYMDEDYIRALEFGLPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEK

Sequences:

>Translated_491_residues
MEELSELLLQRRRKVDALWEAGINPYPNDFKPLHTSADFREAYGTVDTIEDDPRSFTVAGRIIARRSFGKAAFIQIQDRK
GRMQVYVRKDTVGDEAFEAFETFDIGDIVGVGGTPFRTKTGELTLNAAFIRLLTKSLQPLPEKFHGLTDVETRYRQRYVD
LIVNPEVREVFVKRSRIVNLIREFMVRKDFLEVETPMMQPIPGGATARPFVTHHNALDMQLFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVAQEVLGTLDFTYQEMPISFQRPWKRLAVVEAI
LEYGDIDAKSLADRDLAYAYAQRLGLDLPADVGYGKLITEIFEEVAETKLIQPTFITAYPTEVSPLSRKSDKDPEIVDRF
ELFIAGREMANAFSELNDPVDQKERFLAQVAAKAKGDDEAHYMDEDYIRALEFGLPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEK
>Mature_491_residues
MEELSELLLQRRRKVDALWEAGINPYPNDFKPLHTSADFREAYGTVDTIEDDPRSFTVAGRIIARRSFGKAAFIQIQDRK
GRMQVYVRKDTVGDEAFEAFETFDIGDIVGVGGTPFRTKTGELTLNAAFIRLLTKSLQPLPEKFHGLTDVETRYRQRYVD
LIVNPEVREVFVKRSRIVNLIREFMVRKDFLEVETPMMQPIPGGATARPFVTHHNALDMQLFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVAQEVLGTLDFTYQEMPISFQRPWKRLAVVEAI
LEYGDIDAKSLADRDLAYAYAQRLGLDLPADVGYGKLITEIFEEVAETKLIQPTFITAYPTEVSPLSRKSDKDPEIVDRF
ELFIAGREMANAFSELNDPVDQKERFLAQVAAKAKGDDEAHYMDEDYIRALEFGLPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=504, Percent_Identity=41.8650793650794, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=41.8650793650794, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI45439306, Length=478, Percent_Identity=23.6401673640167, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=57.4036511156187, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1789256, Length=493, Percent_Identity=57.2008113590264, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI87082379, Length=323, Percent_Identity=33.4365325077399, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=27.6679841897233, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=502, Percent_Identity=42.0318725099602, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71994340, Length=497, Percent_Identity=42.2535211267606, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535927, Length=502, Percent_Identity=42.0318725099602, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551876, Length=514, Percent_Identity=23.3463035019455, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=212, Percent_Identity=26.8867924528302, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=518, Percent_Identity=41.1196911196911, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=520, Percent_Identity=30, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323011, Length=356, Percent_Identity=25.8426966292135, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=456, Percent_Identity=24.780701754386, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640851, Length=489, Percent_Identity=42.7402862985685, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640849, Length=487, Percent_Identity=42.9158110882957, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24584738, Length=266, Percent_Identity=26.6917293233083, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56311; Mature: 56311

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELSELLLQRRRKVDALWEAGINPYPNDFKPLHTSADFREAYGTVDTIEDDPRSFTVAG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEHHH
RIIARRSFGKAAFIQIQDRKGRMQVYVRKDTVGDEAFEAFETFDIGDIVGVGGTPFRTKT
HHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEECCCCCCCCCCC
GELTLNAAFIRLLTKSLQPLPEKFHGLTDVETRYRQRYVDLIVNPEVREVFVKRSRIVNL
CCEEHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IREFMVRKDFLEVETPMMQPIPGGATARPFVTHHNALDMQLFLRIAPELYLKRLVVGGFE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVAQEVLGTLDFTY
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QEMPISFQRPWKRLAVVEAILEYGDIDAKSLADRDLAYAYAQRLGLDLPADVGYGKLITE
HHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFEEVAETKLIQPTFITAYPTEVSPLSRKSDKDPEIVDRFELFIAGREMANAFSELNDPV
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
DQKERFLAQVAAKAKGDDEAHYMDEDYIRALEFGLPPTAGEGIGIDRLVMLLTDSPSIRD
HHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPQLRKEK
EEECCCCCCCC
>Mature Secondary Structure
MEELSELLLQRRRKVDALWEAGINPYPNDFKPLHTSADFREAYGTVDTIEDDPRSFTVAG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEHHH
RIIARRSFGKAAFIQIQDRKGRMQVYVRKDTVGDEAFEAFETFDIGDIVGVGGTPFRTKT
HHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEECCCCCCCCCCC
GELTLNAAFIRLLTKSLQPLPEKFHGLTDVETRYRQRYVDLIVNPEVREVFVKRSRIVNL
CCEEHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IREFMVRKDFLEVETPMMQPIPGGATARPFVTHHNALDMQLFLRIAPELYLKRLVVGGFE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVAQEVLGTLDFTY
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QEMPISFQRPWKRLAVVEAILEYGDIDAKSLADRDLAYAYAQRLGLDLPADVGYGKLITE
HHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFEEVAETKLIQPTFITAYPTEVSPLSRKSDKDPEIVDRFELFIAGREMANAFSELNDPV
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
DQKERFLAQVAAKAKGDDEAHYMDEDYIRALEFGLPPTAGEGIGIDRLVMLLTDSPSIRD
HHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPQLRKEK
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA