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The map label for this gene is frvX [C]

Identifier: 39997294

GI number: 39997294

Start: 2408911

End: 2409975

Strand: Reverse

Name: frvX [C]

Synonym: GSU2196

Alternate gene names: 39997294

Gene position: 2409975-2408911 (Counterclockwise)

Preceding gene: 39997295

Following gene: 39997293

Centisome position: 63.19

GC content: 63.57

Gene sequence:

>1065_bases
ATGCGTCCGGAATCTCTGGAATTTTTCAAAAAACTTGTGGAGGCCCCCAGCCCTTCCGGCTACGAGCAGCCGGCCCAACG
GGTGTTTCGCGACTACATAGCCCCCTTCGCGGAGGTGAAGACCGATGTCATGGGCAACGTGATCGGTCTCATTCGCGGCG
GGGGGGAGAACCTGCCGCGGGTGATGGTGGTCGGCCACTCGGATGAGATAGGCTTTCAGGTCAAGTACATCGACGACAAC
GGCTTCATCTATTTTGCGCCGATCGGCGGGGTGGACGCCCACCTGACGCCGGGGAAGCGGGTTCGCATCCACACGGCCAA
GGGCGAGGTGGCAGGCGTCATCGGCAAGAAGCCGATCCATTTGATGGACGAGGAAGACCGGCGGAAGGTGGTTCGCCTCG
ACGCCCAGTACATCGACATAGGCGCTACCGACAAGAAGGAGGCCCTGGAGTACGTGCGTGTGGGTGACCCGGTCACCTTT
GCCGCCGGCTTCGAACGCCTGCTGGGCGATCGGGTTGCCTGCCGGGCGTTCGACGACAAGGCCGGCAGCTTCGTGGTGGC
CGAGGTGCTCCGGGCCGTGGCCGAATCGGGGGTGAAACTGCCGGTTGATCTGTACGGCGTCTCCTCGGTACAGGAAGAGA
TCGGGCTGCGCGGCGGCGCAACCAGCGCCTACAGCGTCAATCCCCACGTGGGTATCTGCGTCGAGGTGGACTTCGCCACT
GATCAGCCCGACGTGGAGAAGAAGCACAACGGCGAGGTCAAGCTCGGCAAGGGGCCGATCCTGCCCCGTGGGGCCAATAT
CAATCCGCCTCTCTTTGATCTGCTTTCCTCAACGGCGGAGCGTGAGGAGATCGCTGTCCAGTATACGGGTATCCCCCGGG
CTTCCGGTACCGATGCCAACGTCATGCAGATCTCCCGCGGCGGCGTCGCCACCGCCCTGGTCAAGCTGCCGCTCCGCTAC
ATGCATACGCCGGTGGAGGTGATTTCCCTCTCCGATCTTGATCAGGCGGTCAGGCTGATCGTGGCGGCCTTGCCGGGGAT
GGCGGGCGACTTCACTCCCCGCTGA

Upstream 100 bases:

>100_bases
TCGATCCCGCCGATCTCGGCAAGTATCCCATGTGACATCAGGGCAGCGGCCGGCAGGCCGTGGCCCGTGCCCCTGACCCG
CTATCGTGGAGGATTCTTTC

Downstream 100 bases:

>100_bases
CCGCGCACCATAGACCGGGGCCGGGGAACGTATACTCTCCGGCCCCGGTTTTTCCCCTTGACTCATCCTTGTCCCTCCAC
TATTCTTTTCGGTTAATACT

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRPESLEFFKKLVEAPSPSGYEQPAQRVFRDYIAPFAEVKTDVMGNVIGLIRGGGENLPRVMVVGHSDEIGFQVKYIDDN
GFIYFAPIGGVDAHLTPGKRVRIHTAKGEVAGVIGKKPIHLMDEEDRRKVVRLDAQYIDIGATDKKEALEYVRVGDPVTF
AAGFERLLGDRVACRAFDDKAGSFVVAEVLRAVAESGVKLPVDLYGVSSVQEEIGLRGGATSAYSVNPHVGICVEVDFAT
DQPDVEKKHNGEVKLGKGPILPRGANINPPLFDLLSSTAEREEIAVQYTGIPRASGTDANVMQISRGGVATALVKLPLRY
MHTPVEVISLSDLDQAVRLIVAALPGMAGDFTPR

Sequences:

>Translated_354_residues
MRPESLEFFKKLVEAPSPSGYEQPAQRVFRDYIAPFAEVKTDVMGNVIGLIRGGGENLPRVMVVGHSDEIGFQVKYIDDN
GFIYFAPIGGVDAHLTPGKRVRIHTAKGEVAGVIGKKPIHLMDEEDRRKVVRLDAQYIDIGATDKKEALEYVRVGDPVTF
AAGFERLLGDRVACRAFDDKAGSFVVAEVLRAVAESGVKLPVDLYGVSSVQEEIGLRGGATSAYSVNPHVGICVEVDFAT
DQPDVEKKHNGEVKLGKGPILPRGANINPPLFDLLSSTAEREEIAVQYTGIPRASGTDANVMQISRGGVATALVKLPLRY
MHTPVEVISLSDLDQAVRLIVAALPGMAGDFTPR
>Mature_354_residues
MRPESLEFFKKLVEAPSPSGYEQPAQRVFRDYIAPFAEVKTDVMGNVIGLIRGGGENLPRVMVVGHSDEIGFQVKYIDDN
GFIYFAPIGGVDAHLTPGKRVRIHTAKGEVAGVIGKKPIHLMDEEDRRKVVRLDAQYIDIGATDKKEALEYVRVGDPVTF
AAGFERLLGDRVACRAFDDKAGSFVVAEVLRAVAESGVKLPVDLYGVSSVQEEIGLRGGATSAYSVNPHVGICVEVDFAT
DQPDVEKKHNGEVKLGKGPILPRGANINPPLFDLLSSTAEREEIAVQYTGIPRASGTDANVMQISRGGVATALVKLPLRY
MHTPVEVISLSDLDQAVRLIVAALPGMAGDFTPR

Specific function: Hydrolyzes di-, tri- and tetrapeptides with a lysine as the N-terminal amino acid and with Gly, Lys, Ala, Phe or Glu in the second position [H]

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=352, Percent_Identity=31.8181818181818, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082417, Length=357, Percent_Identity=31.9327731092437, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788727, Length=347, Percent_Identity=30.2593659942363, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: =3.4.11.15 [H]

Molecular weight: Translated: 38248; Mature: 38248

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPESLEFFKKLVEAPSPSGYEQPAQRVFRDYIAPFAEVKTDVMGNVIGLIRGGGENLPR
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCE
VMVVGHSDEIGFQVKYIDDNGFIYFAPIGGVDAHLTPGKRVRIHTAKGEVAGVIGKKPIH
EEEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCEEEEEECCCCEEEECCCCCEE
LMDEEDRRKVVRLDAQYIDIGATDKKEALEYVRVGDPVTFAAGFERLLGDRVACRAFDDK
ECCCHHHHHEEEECCEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCHHEEEEECCC
AGSFVVAEVLRAVAESGVKLPVDLYGVSSVQEEIGLRGGATSAYSVNPHVGICVEVDFAT
CCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCCCCCCEEECCCCEEEEEEEECCC
DQPDVEKKHNGEVKLGKGPILPRGANINPPLFDLLSSTAEREEIAVQYTGIPRASGTDAN
CCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
VMQISRGGVATALVKLPLRYMHTPVEVISLSDLDQAVRLIVAALPGMAGDFTPR
EEEEECCCHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRPESLEFFKKLVEAPSPSGYEQPAQRVFRDYIAPFAEVKTDVMGNVIGLIRGGGENLPR
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCE
VMVVGHSDEIGFQVKYIDDNGFIYFAPIGGVDAHLTPGKRVRIHTAKGEVAGVIGKKPIH
EEEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCEEEEEECCCCEEEECCCCCEE
LMDEEDRRKVVRLDAQYIDIGATDKKEALEYVRVGDPVTFAAGFERLLGDRVACRAFDDK
ECCCHHHHHEEEECCEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCHHEEEEECCC
AGSFVVAEVLRAVAESGVKLPVDLYGVSSVQEEIGLRGGATSAYSVNPHVGICVEVDFAT
CCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCCCCCCEEECCCCEEEEEEEECCC
DQPDVEKKHNGEVKLGKGPILPRGANINPPLFDLLSSTAEREEIAVQYTGIPRASGTDAN
CCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
VMQISRGGVATALVKLPLRYMHTPVEVISLSDLDQAVRLIVAALPGMAGDFTPR
EEEEECCCHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA