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The map label for this gene is mrdA [H]

Identifier: 39997177

GI number: 39997177

Start: 2285312

End: 2287231

Strand: Reverse

Name: mrdA [H]

Synonym: GSU2079

Alternate gene names: 39997177

Gene position: 2287231-2285312 (Counterclockwise)

Preceding gene: 39997178

Following gene: 39997176

Centisome position: 59.97

GC content: 60.99

Gene sequence:

>1920_bases
ATGAACGGTCAGCGCTTCATACGCGATACGCAGGAGCCGAAGCGACAGCTGGTCGTGCTGCTTATCGCGGTGGGGGTGTT
TTTCTTCATCCTCGTCACGCGGCTCTGGTACCTCCAGATTATCAAGGCTGAGGATTTTCAGAGCCTTTCCGAAAACAACC
GGCTGCGCCTCGTGCCCGTCGCGGCGTCCCGCGGGACCATATTCGACCGTAACGGAGCGGTCCTGGTGAGCAACCGGCCT
TCCTTCAGCGTTGCCGTCATTCCCCAGGAAGTCCGCGATCGCGATTGGCTGGTCGATCAACTGGTTACCCTCCTTGGTGC
CGACAGGGATGAACTCGTCAAAAAGTGGGAGAAAGGGAAGGGACGTGCCCGCTACTATCCCATCGTTCTCGCATCGGGGA
TCACCCGTGACCAACTGGAGCTTCTGGAAGAGAAACGGCTCTGGCTCCCCGGCATCGAGGTCGAGATGAAGCCGGTACGT
CAGTATGAGAACGGTACACTGGCGGCTCACCTGCTGGGCTACATCGGTGAAATCTCGGACGAAGAGCTGAAAAACCCGGC
CTTTGTCGGCTACAATTCAGGCGATTACCTGGGCAAGAGCGGGATTGAGCGCAGCTGGGAAGAGGTGCTCCACGGCCACG
ACGGTGGACGGCAGATCGAGGTAGACGCCCGGGGGCGGATCCTCAGGACAATTGCCGAGACGCCCCCCACCATGGGGTCG
AGCCTGGTCCTGACCATCGACGCGCAACTTCAGAAGCGGGCGGAGCAGGCTTTTGGCGACCAGGCGGGAGCTGCGGTGGT
CATGGAAGTGAACACCGGCGAAATTCTGGCATTCGTCAGCAATCCCGAATTCGATCCGGCTCTGTTCGCCGGCAGGATGC
CCCCCGAAATCTGGAAACAATACCTGGAAGACAAGCGCCATCCCCTGGAGAACAAGGCCTTGAAGGGGCAGTATCCGCCG
GGATCGACCTACAAGATCATCACCGCTCTGGCAGGGCTTGAAGAGGGGATCATCGACGACCACACTACGGTAACCTGCAA
GGGTTCTTATGCGCTAGGGAATACCACGTTTCGCTGCTGGGACAAGAAGGGGCACGGAGCGGTCGATCTGAGGCGAGCCC
TTCGCGAGTCGTGCGACGTCTACTTCTATGTGCTCGGCGAGAAGCTCGGCATTGACCGGCTTTCCGCCTATGCCCAAAAG
TTTGCCCTCGGTGCGCCCATGGGGGTCGGCCTGGACAATGAAAAGGGTGGGCTCATCCCCACCTCCGCCTGGAAGCTTCA
GCGGCACAAAACCAGGTGGTTCCAGGGAGATACGCTGAGTGCGGCCATCGGCCAGGGCTACGTCCTCATGACCCCCATCC
AGCTGGCATCAATGACCGCTGCCATTGCCACGGACGGTATCGTGTACCGGCCCCGGCTGGTAAAGCGGATCATTGACCGC
GACGGCAAGGTCCTCAGGGAGTTTCAGCCGGAGATCATCACCCGGGCCGATATTTCGCCGCGCAGTTTCAAGTCGGTCAA
GGAAGGACTGTGGGCTGTCGTGAACGAGTCGGGTGGTACCGGCGGCATGTCACGGATTCCTGATGTCCGGGTCGCCGGCA
AGACAGGGACTTCTCAGGTCGTCAAGCTGAGAGATACGCGCGGGTTTATCCCCTATCAGTATCGCGATCACGCCCTGTTC
GTGGCCTACGCCCCCTATGAGCACCCCGAAGTTGCTGTTGCGGTGGTGATCGAACACGGCGAGCACGGAGGCTCGGCGGC
GGCACCCATTGCCGGCAGCATTCTGCGCGCCTACTTCGAAGGGAAGGGGGTCATCCGCCGACCGGTCAAAGCCGGGAAGG
GAACTGCCGCCGGGGACGACGGCTCCGGGGAAAACGCGACATCCGACGAAGGGGCGCAGCCCCGCGAGGATCAGGAATAG

Upstream 100 bases:

>100_bases
CTTCGCTGCTCGAAGGCCTGGTTCCCCAGAGCCTCGTGAATGCCCTTGTCGCCTCCATCGTCTTCACCTTCACCCCCCTT
GCACGGTTGGGGGACATTCG

Downstream 100 bases:

>100_bases
ACAATGATCGACCGACGACTCATCACCAACTTCGACTGGATGCTGCTGGGACTCGTGCTGCTTATCTGTGCGGTAGGAGT
TGTGAATATCTACAGCGCCT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MNGQRFIRDTQEPKRQLVVLLIAVGVFFFILVTRLWYLQIIKAEDFQSLSENNRLRLVPVAASRGTIFDRNGAVLVSNRP
SFSVAVIPQEVRDRDWLVDQLVTLLGADRDELVKKWEKGKGRARYYPIVLASGITRDQLELLEEKRLWLPGIEVEMKPVR
QYENGTLAAHLLGYIGEISDEELKNPAFVGYNSGDYLGKSGIERSWEEVLHGHDGGRQIEVDARGRILRTIAETPPTMGS
SLVLTIDAQLQKRAEQAFGDQAGAAVVMEVNTGEILAFVSNPEFDPALFAGRMPPEIWKQYLEDKRHPLENKALKGQYPP
GSTYKIITALAGLEEGIIDDHTTVTCKGSYALGNTTFRCWDKKGHGAVDLRRALRESCDVYFYVLGEKLGIDRLSAYAQK
FALGAPMGVGLDNEKGGLIPTSAWKLQRHKTRWFQGDTLSAAIGQGYVLMTPIQLASMTAAIATDGIVYRPRLVKRIIDR
DGKVLREFQPEIITRADISPRSFKSVKEGLWAVVNESGGTGGMSRIPDVRVAGKTGTSQVVKLRDTRGFIPYQYRDHALF
VAYAPYEHPEVAVAVVIEHGEHGGSAAAPIAGSILRAYFEGKGVIRRPVKAGKGTAAGDDGSGENATSDEGAQPREDQE

Sequences:

>Translated_639_residues
MNGQRFIRDTQEPKRQLVVLLIAVGVFFFILVTRLWYLQIIKAEDFQSLSENNRLRLVPVAASRGTIFDRNGAVLVSNRP
SFSVAVIPQEVRDRDWLVDQLVTLLGADRDELVKKWEKGKGRARYYPIVLASGITRDQLELLEEKRLWLPGIEVEMKPVR
QYENGTLAAHLLGYIGEISDEELKNPAFVGYNSGDYLGKSGIERSWEEVLHGHDGGRQIEVDARGRILRTIAETPPTMGS
SLVLTIDAQLQKRAEQAFGDQAGAAVVMEVNTGEILAFVSNPEFDPALFAGRMPPEIWKQYLEDKRHPLENKALKGQYPP
GSTYKIITALAGLEEGIIDDHTTVTCKGSYALGNTTFRCWDKKGHGAVDLRRALRESCDVYFYVLGEKLGIDRLSAYAQK
FALGAPMGVGLDNEKGGLIPTSAWKLQRHKTRWFQGDTLSAAIGQGYVLMTPIQLASMTAAIATDGIVYRPRLVKRIIDR
DGKVLREFQPEIITRADISPRSFKSVKEGLWAVVNESGGTGGMSRIPDVRVAGKTGTSQVVKLRDTRGFIPYQYRDHALF
VAYAPYEHPEVAVAVVIEHGEHGGSAAAPIAGSILRAYFEGKGVIRRPVKAGKGTAAGDDGSGENATSDEGAQPREDQE
>Mature_639_residues
MNGQRFIRDTQEPKRQLVVLLIAVGVFFFILVTRLWYLQIIKAEDFQSLSENNRLRLVPVAASRGTIFDRNGAVLVSNRP
SFSVAVIPQEVRDRDWLVDQLVTLLGADRDELVKKWEKGKGRARYYPIVLASGITRDQLELLEEKRLWLPGIEVEMKPVR
QYENGTLAAHLLGYIGEISDEELKNPAFVGYNSGDYLGKSGIERSWEEVLHGHDGGRQIEVDARGRILRTIAETPPTMGS
SLVLTIDAQLQKRAEQAFGDQAGAAVVMEVNTGEILAFVSNPEFDPALFAGRMPPEIWKQYLEDKRHPLENKALKGQYPP
GSTYKIITALAGLEEGIIDDHTTVTCKGSYALGNTTFRCWDKKGHGAVDLRRALRESCDVYFYVLGEKLGIDRLSAYAQK
FALGAPMGVGLDNEKGGLIPTSAWKLQRHKTRWFQGDTLSAAIGQGYVLMTPIQLASMTAAIATDGIVYRPRLVKRIIDR
DGKVLREFQPEIITRADISPRSFKSVKEGLWAVVNESGGTGGMSRIPDVRVAGKTGTSQVVKLRDTRGFIPYQYRDHALF
VAYAPYEHPEVAVAVVIEHGEHGGSAAAPIAGSILRAYFEGKGVIRRPVKAGKGTAAGDDGSGENATSDEGAQPREDQE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=595, Percent_Identity=35.2941176470588, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1786272, Length=614, Percent_Identity=27.1986970684039, Blast_Score=165, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70532; Mature: 70532

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGQRFIRDTQEPKRQLVVLLIAVGVFFFILVTRLWYLQIIKAEDFQSLSENNRLRLVPV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
AASRGTIFDRNGAVLVSNRPSFSVAVIPQEVRDRDWLVDQLVTLLGADRDELVKKWEKGK
ECCCCEEEECCCEEEEECCCCEEEEECCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
GRARYYPIVLASGITRDQLELLEEKRLWLPGIEVEMKPVRQYENGTLAAHLLGYIGEISD
CCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHCCCH
EELKNPAFVGYNSGDYLGKSGIERSWEEVLHGHDGGRQIEVDARGRILRTIAETPPTMGS
HHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCC
SLVLTIDAQLQKRAEQAFGDQAGAAVVMEVNTGEILAFVSNPEFDPALFAGRMPPEIWKQ
EEEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHCCCCCHHHHHH
YLEDKRHPLENKALKGQYPPGSTYKIITALAGLEEGIIDDHTTVTCKGSYALGNTTFRCW
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCEEEEE
DKKGHGAVDLRRALRESCDVYFYVLGEKLGIDRLSAYAQKFALGAPMGVGLDNEKGGLIP
CCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TSAWKLQRHKTRWFQGDTLSAAIGQGYVLMTPIQLASMTAAIATDGIVYRPRLVKRIIDR
CHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHCCCEEECHHHHHHHHCC
DGKVLREFQPEIITRADISPRSFKSVKEGLWAVVNESGGTGGMSRIPDVRVAGKTGTSQV
CCHHHHHCCHHHEEECCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCCCCCE
VKLRDTRGFIPYQYRDHALFVAYAPYEHPEVAVAVVIEHGEHGGSAAAPIAGSILRAYFE
EEEECCCCCCCEEECCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHC
GKGVIRRPVKAGKGTAAGDDGSGENATSDEGAQPREDQE
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGQRFIRDTQEPKRQLVVLLIAVGVFFFILVTRLWYLQIIKAEDFQSLSENNRLRLVPV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
AASRGTIFDRNGAVLVSNRPSFSVAVIPQEVRDRDWLVDQLVTLLGADRDELVKKWEKGK
ECCCCEEEECCCEEEEECCCCEEEEECCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
GRARYYPIVLASGITRDQLELLEEKRLWLPGIEVEMKPVRQYENGTLAAHLLGYIGEISD
CCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHCCCH
EELKNPAFVGYNSGDYLGKSGIERSWEEVLHGHDGGRQIEVDARGRILRTIAETPPTMGS
HHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCC
SLVLTIDAQLQKRAEQAFGDQAGAAVVMEVNTGEILAFVSNPEFDPALFAGRMPPEIWKQ
EEEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCHHHHCCCCCHHHHHH
YLEDKRHPLENKALKGQYPPGSTYKIITALAGLEEGIIDDHTTVTCKGSYALGNTTFRCW
HHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCEEEEE
DKKGHGAVDLRRALRESCDVYFYVLGEKLGIDRLSAYAQKFALGAPMGVGLDNEKGGLIP
CCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TSAWKLQRHKTRWFQGDTLSAAIGQGYVLMTPIQLASMTAAIATDGIVYRPRLVKRIIDR
CHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHCCCEEECHHHHHHHHCC
DGKVLREFQPEIITRADISPRSFKSVKEGLWAVVNESGGTGGMSRIPDVRVAGKTGTSQV
CCHHHHHCCHHHEEECCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCCCCCE
VKLRDTRGFIPYQYRDHALFVAYAPYEHPEVAVAVVIEHGEHGGSAAAPIAGSILRAYFE
EEEECCCCCCCEEECCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHC
GKGVIRRPVKAGKGTAAGDDGSGENATSDEGAQPREDQE
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]