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The map label for this gene is icaA [H]

Identifier: 39997075

GI number: 39997075

Start: 2167831

End: 2168973

Strand: Reverse

Name: icaA [H]

Synonym: GSU1977

Alternate gene names: 39997075

Gene position: 2168973-2167831 (Counterclockwise)

Preceding gene: 39997076

Following gene: 39997074

Centisome position: 56.87

GC content: 62.9

Gene sequence:

>1143_bases
ATGATTGAACTGGTGCTGCTCATATCGCTCTTCATGGTGTTCTACGTGTATGGAGGATACCCGGTGCTGGCCGCAGCGCT
CGGTCTGGTCGCGGGGCGTCCGGTTCGCAAGGGCTCCATCGAGCCCATGGTTACCATCGTCATTGCCGCATACAACGAGG
AAGACGTCATCGGTGCCACCATTGAGAACAAGCTGGCACTGGACTATCCCCAGGGCCGCCTCGAAATCATCGTGGTTTCC
GATGGCTCTACCGACCGTACCGACGAGATTGTCGGCATGTATGCCGTGCGGAACGTCAGGCTGATCCGCCAGGAGCCCCG
CGCCGGCAAAACAAGCGGCCTCAACCTGGCAATCCCCCAGGCGCGTGGCGAGATCATTGTCTTTTCCGATGCCAATTCGC
TCTATGCTCCTTCGGCCCTGCGCCACCTGGTGGCGAACTTTGCCGACGAGGAGGTGGGCTACGTGACCGGCCGGATGATG
TATGCCAATCCCGACGGCACCACCATCGGCGAGGGGTGCAGCACCTACATGCGCTATGAGAACTATTTACGCGTCATCGA
GAGCCGCATCGGATCGGTGGTCGGGGTGGACGGCGGGGTCGACGCCATGCGCAAGCGGCTCTACCGCCCCATGAATGCCG
ACCAGCTCCCGGATTTTGTCCAGCCGCTCAAGGTCGTCGAGCAGGGGTACCGTGTCGTGTACGAGCCCGAAGCGCTCCTG
TGGGAGCCGTCTCTCAAGGAGGCCGGCGACGAGTACCGGATGCGGGTCCGCGTTTCCTTGCGGGCTTTCTGGGCCCTGTT
CGACATGAGAAAGCTCCTGGCCCCGTGGCACGATCCCCTGTTCGCCTGGCAGCTCTGGTCGCACAAGGCCCTGCGTTACC
TCTGCTTCCTGTTCCTGGTGGCCGCTTATGTCTCCAACCTGGCCCTGCTGGGCAAGGGAACCGGGTACGTGGCCCTGTTT
CTCCTCCAGAGCGCCGGCTATCTCGCGGCGGTGACCATGCCCTACCTGGAGCGGACCGGCAAGGGGTGCCGTCTCTGCAC
CCTGGCCCGCTACTTTTTCCTGCTGAACCTTGCCGCCGCCCACGCCTTCGGCAAGTTCCTCAGGGGGGAGAAACAGGTCA
TCTGGACGCCGCGGAAGGGGTAG

Upstream 100 bases:

>100_bases
CAACCGCCCGTCCCCTGGAGCGTATCACGCATTTCGTCGAAGCGTTTTATCCCTCCTTCCACCGCTACGCGGCTGGGGCC
GGTCCGTTGGAGACGAGATA

Downstream 100 bases:

>100_bases
CATGAAGGTTTTGCACGTCATCGACAGCGGTGGCCTGTACGGAGCCGAAGTCATGCTCCTCAATCTCGCTGCCGAGCAGG
CCGCCATGGGGCTTGAACCG

Product: glycosyl transferase, group 2 family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIELVLLISLFMVFYVYGGYPVLAAALGLVAGRPVRKGSIEPMVTIVIAAYNEEDVIGATIENKLALDYPQGRLEIIVVS
DGSTDRTDEIVGMYAVRNVRLIRQEPRAGKTSGLNLAIPQARGEIIVFSDANSLYAPSALRHLVANFADEEVGYVTGRMM
YANPDGTTIGEGCSTYMRYENYLRVIESRIGSVVGVDGGVDAMRKRLYRPMNADQLPDFVQPLKVVEQGYRVVYEPEALL
WEPSLKEAGDEYRMRVRVSLRAFWALFDMRKLLAPWHDPLFAWQLWSHKALRYLCFLFLVAAYVSNLALLGKGTGYVALF
LLQSAGYLAAVTMPYLERTGKGCRLCTLARYFFLLNLAAAHAFGKFLRGEKQVIWTPRKG

Sequences:

>Translated_380_residues
MIELVLLISLFMVFYVYGGYPVLAAALGLVAGRPVRKGSIEPMVTIVIAAYNEEDVIGATIENKLALDYPQGRLEIIVVS
DGSTDRTDEIVGMYAVRNVRLIRQEPRAGKTSGLNLAIPQARGEIIVFSDANSLYAPSALRHLVANFADEEVGYVTGRMM
YANPDGTTIGEGCSTYMRYENYLRVIESRIGSVVGVDGGVDAMRKRLYRPMNADQLPDFVQPLKVVEQGYRVVYEPEALL
WEPSLKEAGDEYRMRVRVSLRAFWALFDMRKLLAPWHDPLFAWQLWSHKALRYLCFLFLVAAYVSNLALLGKGTGYVALF
LLQSAGYLAAVTMPYLERTGKGCRLCTLARYFFLLNLAAAHAFGKFLRGEKQVIWTPRKG
>Mature_380_residues
MIELVLLISLFMVFYVYGGYPVLAAALGLVAGRPVRKGSIEPMVTIVIAAYNEEDVIGATIENKLALDYPQGRLEIIVVS
DGSTDRTDEIVGMYAVRNVRLIRQEPRAGKTSGLNLAIPQARGEIIVFSDANSLYAPSALRHLVANFADEEVGYVTGRMM
YANPDGTTIGEGCSTYMRYENYLRVIESRIGSVVGVDGGVDAMRKRLYRPMNADQLPDFVQPLKVVEQGYRVVYEPEALL
WEPSLKEAGDEYRMRVRVSLRAFWALFDMRKLLAPWHDPLFAWQLWSHKALRYLCFLFLVAAYVSNLALLGKGTGYVALF
LLQSAGYLAAVTMPYLERTGKGCRLCTLARYFFLLNLAAAHAFGKFLRGEKQVIWTPRKG

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=358, Percent_Identity=24.5810055865922, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 42522; Mature: 42522

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELVLLISLFMVFYVYGGYPVLAAALGLVAGRPVRKGSIEPMVTIVIAAYNEEDVIGAT
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEE
IENKLALDYPQGRLEIIVVSDGSTDRTDEIVGMYAVRNVRLIRQEPRAGKTSGLNLAIPQ
CCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
ARGEIIVFSDANSLYAPSALRHLVANFADEEVGYVTGRMMYANPDGTTIGEGCSTYMRYE
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
NYLRVIESRIGSVVGVDGGVDAMRKRLYRPMNADQLPDFVQPLKVVEQGYRVVYEPEALL
HHHHHHHHHHCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCEE
WEPSLKEAGDEYRMRVRVSLRAFWALFDMRKLLAPWHDPLFAWQLWSHKALRYLCFLFLV
ECCCHHHCCCHHEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAYVSNLALLGKGTGYVALFLLQSAGYLAAVTMPYLERTGKGCRLCTLARYFFLLNLAAA
HHHHHCEEEECCCCCHHHHHHHHCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HAFGKFLRGEKQVIWTPRKG
HHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure
MIELVLLISLFMVFYVYGGYPVLAAALGLVAGRPVRKGSIEPMVTIVIAAYNEEDVIGAT
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCEEEE
IENKLALDYPQGRLEIIVVSDGSTDRTDEIVGMYAVRNVRLIRQEPRAGKTSGLNLAIPQ
CCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
ARGEIIVFSDANSLYAPSALRHLVANFADEEVGYVTGRMMYANPDGTTIGEGCSTYMRYE
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHH
NYLRVIESRIGSVVGVDGGVDAMRKRLYRPMNADQLPDFVQPLKVVEQGYRVVYEPEALL
HHHHHHHHHHCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCEE
WEPSLKEAGDEYRMRVRVSLRAFWALFDMRKLLAPWHDPLFAWQLWSHKALRYLCFLFLV
ECCCHHHCCCHHEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAYVSNLALLGKGTGYVALFLLQSAGYLAAVTMPYLERTGKGCRLCTLARYFFLLNLAAA
HHHHHCEEEECCCCCHHHHHHHHCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HAFGKFLRGEKQVIWTPRKG
HHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]