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The map label for this gene is strE [H]

Identifier: 39997073

GI number: 39997073

Start: 2165691

End: 2166701

Strand: Reverse

Name: strE [H]

Synonym: GSU1975

Alternate gene names: 39997073

Gene position: 2166701-2165691 (Counterclockwise)

Preceding gene: 39997074

Following gene: 39997072

Centisome position: 56.81

GC content: 59.15

Gene sequence:

>1011_bases
ATGGATCTCAAAGGCAAAAAGATACTTGTAACCGGCGCCGATGGCTTCATCGGTTCACATCTCACCGAAGCACTCCTGAT
GCGGGGGTACGATACGCGGGCATTCGTGTACTACAACTCGTTCAACTCCTGGGGATGGCTTGACCACCTGGACCCGGAAC
TGCTGAAATCGCTGGATGTCTTTGCCGGCGACATCCGCGATCCCCACGGGGTCAGGGAGGCCATGAAGGGGTGCGACGTG
GTCCTCCACCTGGCGGCGCTCATCGCAATCCCCTATTCGTATCACTCACCGGATACCTACGTGGACACCAACGTCAAGGG
AACCCTCAACGTGGTCCAGGCGGCCCGGGAGTTGGGGGTAGCCAAGGTCGTCCATACGTCCACCAGCGAAGTGTACGGCA
CTGCCCGATTCGTCCCCATCACCGAGGAACACCCGCTCCAGGGTCAATCACCGTACTCCGCGTCCAAGATCGGTGCAGAC
CAGATCGCCATGTCCTTCTACAGTTCGTTCGACACACCCGTCGCCATTATCCGTCCCTTCAATACCTATGGACCGAGACA
GTCGGCGCGGGCATTCATCCCAACCGTCATCACCCAGATCGCCTCCGGCGCCCGTACCCTCAGATTGGGAGCCCTGCATC
CGACCCGCGACCTGAATTACGTGGCCGACACCGTCGCGGGCTTCATCGCGGTGGCTGAGAGCGAGAAGTCGGTGGGGGAA
GTGATAAACATCGGCAGCAACTTCGAGATTTCCATGGGTGAGACCGCCCGAATGATAGCCGATGTCATGGGCGCCGATGT
GGAGATCGTGACTGATGCGGAACGTCTCAGGCCGGACAAGAGCGAGGTCGAACGGCTTTGGGCCGACACGTCCAAGGCAA
AAAGGCTCCTGGATCATGGGCAGAACTATGGCGGGAAGGACGGATTGCGCAGGGGGCTCGTCGAGACGGTCGAGTGGTTC
GTCCGACCGGAGAATCTCAAAGCTTACAAGGCCAATATCTACAACATCTGA

Upstream 100 bases:

>100_bases
ACACTTGGTCGAAACACGCTACGCAGCCGGCGCGATGGCCATCAAATACAGCGAAATCTATGACGGTGTTCATCCCGCCA
TACATCGAAAGTGAGCATTA

Downstream 100 bases:

>100_bases
AAGACCGCGAAAGGGACCCGATGATTCCGCTGTCAGTGCCAACCCTTAAAGGGAACGAATGGAAATACGTCAAGGAATGC
CTGGATACGGGATGGGTATC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDLKGKKILVTGADGFIGSHLTEALLMRGYDTRAFVYYNSFNSWGWLDHLDPELLKSLDVFAGDIRDPHGVREAMKGCDV
VLHLAALIAIPYSYHSPDTYVDTNVKGTLNVVQAARELGVAKVVHTSTSEVYGTARFVPITEEHPLQGQSPYSASKIGAD
QIAMSFYSSFDTPVAIIRPFNTYGPRQSARAFIPTVITQIASGARTLRLGALHPTRDLNYVADTVAGFIAVAESEKSVGE
VINIGSNFEISMGETARMIADVMGADVEIVTDAERLRPDKSEVERLWADTSKAKRLLDHGQNYGGKDGLRRGLVETVEWF
VRPENLKAYKANIYNI

Sequences:

>Translated_336_residues
MDLKGKKILVTGADGFIGSHLTEALLMRGYDTRAFVYYNSFNSWGWLDHLDPELLKSLDVFAGDIRDPHGVREAMKGCDV
VLHLAALIAIPYSYHSPDTYVDTNVKGTLNVVQAARELGVAKVVHTSTSEVYGTARFVPITEEHPLQGQSPYSASKIGAD
QIAMSFYSSFDTPVAIIRPFNTYGPRQSARAFIPTVITQIASGARTLRLGALHPTRDLNYVADTVAGFIAVAESEKSVGE
VINIGSNFEISMGETARMIADVMGADVEIVTDAERLRPDKSEVERLWADTSKAKRLLDHGQNYGGKDGLRRGLVETVEWF
VRPENLKAYKANIYNI
>Mature_336_residues
MDLKGKKILVTGADGFIGSHLTEALLMRGYDTRAFVYYNSFNSWGWLDHLDPELLKSLDVFAGDIRDPHGVREAMKGCDV
VLHLAALIAIPYSYHSPDTYVDTNVKGTLNVVQAARELGVAKVVHTSTSEVYGTARFVPITEEHPLQGQSPYSASKIGAD
QIAMSFYSSFDTPVAIIRPFNTYGPRQSARAFIPTVITQIASGARTLRLGALHPTRDLNYVADTVAGFIAVAESEKSVGE
VINIGSNFEISMGETARMIADVMGADVEIVTDAERLRPDKSEVERLWADTSKAKRLLDHGQNYGGKDGLRRGLVETVEWF
VRPENLKAYKANIYNI

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=348, Percent_Identity=30.7471264367816, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI42516563, Length=302, Percent_Identity=29.1390728476821, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI56237023, Length=325, Percent_Identity=26.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI56118217, Length=325, Percent_Identity=26.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI189083684, Length=325, Percent_Identity=26.7692307692308, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4504031, Length=320, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI193211614, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI8393516, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786974, Length=317, Percent_Identity=29.3375394321767, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=28.6931818181818, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=29.4797687861272, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788366, Length=333, Percent_Identity=28.2282282282282, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788365, Length=349, Percent_Identity=22.6361031518625, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=28.0120481927711, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=26.7477203647416, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=325, Percent_Identity=26.7692307692308, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=326, Percent_Identity=27.3006134969325, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=327, Percent_Identity=23.5474006116208, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=327, Percent_Identity=23.5474006116208, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=327, Percent_Identity=23.5474006116208, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=327, Percent_Identity=23.5474006116208, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=327, Percent_Identity=23.5474006116208, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71987463, Length=197, Percent_Identity=32.48730964467, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=267, Percent_Identity=25.4681647940075, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=325, Percent_Identity=23.6923076923077, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=325, Percent_Identity=28, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=29.0519877675841, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=27.2980501392758, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24158427, Length=328, Percent_Identity=25.3048780487805, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36958; Mature: 36958

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKGKKILVTGADGFIGSHLTEALLMRGYDTRAFVYYNSFNSWGWLDHLDPELLKSLDV
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
FAGDIRDPHGVREAMKGCDVVLHLAALIAIPYSYHSPDTYVDTNVKGTLNVVQAARELGV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHCCH
AKVVHTSTSEVYGTARFVPITEEHPLQGQSPYSASKIGADQIAMSFYSSFDTPVAIIRPF
HHEEECCCHHHCCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEECC
NTYGPRQSARAFIPTVITQIASGARTLRLGALHPTRDLNYVADTVAGFIAVAESEKSVGE
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
VINIGSNFEISMGETARMIADVMGADVEIVTDAERLRPDKSEVERLWADTSKAKRLLDHG
HEECCCCEEEECCHHHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHCHHHHHHHHHCC
QNYGGKDGLRRGLVETVEWFVRPENLKAYKANIYNI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MDLKGKKILVTGADGFIGSHLTEALLMRGYDTRAFVYYNSFNSWGWLDHLDPELLKSLDV
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
FAGDIRDPHGVREAMKGCDVVLHLAALIAIPYSYHSPDTYVDTNVKGTLNVVQAARELGV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHCCH
AKVVHTSTSEVYGTARFVPITEEHPLQGQSPYSASKIGADQIAMSFYSSFDTPVAIIRPF
HHEEECCCHHHCCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEECC
NTYGPRQSARAFIPTVITQIASGARTLRLGALHPTRDLNYVADTVAGFIAVAESEKSVGE
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
VINIGSNFEISMGETARMIADVMGADVEIVTDAERLRPDKSEVERLWADTSKAKRLLDHG
HEECCCCEEEECCHHHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHCHHHHHHHHHCC
QNYGGKDGLRRGLVETVEWFVRPENLKAYKANIYNI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661369 [H]